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Nine species of the genus Calypogeia Raddi are currently known from Europe: C. azurea, C. integristipula, C. neesiana,C. suecica, C. muelleriana, C. sphagnicola, C. fissa, C. arguta, and C. azorica. Recently, another species, morphologically resembling C. muelleriana but genetically distinct from it, was detected using isozyme markers. In the present study, relationships between the newly detected species (C. sp. nov.) and typical C. muelleriana were analyzed using the DNA sequencesdata of three regions from the chloroplast genome: introns of trnG and trnL genes and intergenic spacer trnH-psbA. Calypogeia sp. nov. differs from C. muelleriana s. str. (typical form) in all examined chloroplast regions. It differs as well from C. azurea, which was used as a reference species. The number of fixed nucleotide differences between C. muelleriana s. str. and C. sp. nov. is almost the same as between C. muelleriana s. str. and C. azurea. The results of the present study suggest a closer affinity of C. sp. nov. to C. azurea than to C. muelleriana s. str. in Europe, C. muelleriana s. str. was noted in Poland, Germany, Holland, United Kingdom and Azores. Samples determined as C. sp. nov., besides Poland, were so far detected also in North America.
The main aim of this study was to describe the variation between the populations of the dwarf mountain pine Pinus mugo Turra based on the morphological and anatomical traits of their needles, and to investigate the relationship between the observed variation and environmental conditions (altitude and substrate).Two-year-old needles were collected from 180 individuals of six populations of P. mugo growing in the Tatra Mts. Two populations were classified as dense, located at 1360–1450 m altitude, and the remaining four formed loose clusters and were situated at 1500–1650 m altitude. Four of the populations are growing on granite and two on a limestone substrate. The natural variation of 10 morpho-anatomical and 3 synthetic needle traits was measured. In addition to descriptive statistics, the analyses of variance (ANOVA) with a Tukey test and principal component analysis were computed. We also estimated Pearson correlation coefficients for the examined needle traits and altitude as well as substrate. Our results indicate that the P. mugo populations differ significantly with regard to the investigated traits for which the Trzydniowiański Wierch population was the most distinct. The observed pattern of variability is largely caused by differences in stomatal traits and these features are positive correlated with altitude. Additionally, populations growing on granite have larger values for most of the examined traits compared to populations growing on limestone.
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