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Indoor air composition inside the cabins of five new vehicles was examined. Air pollution was assessed on the basis of determination of volatile organic compound (VOC) concentrations, which were emitted from interior materials. Air samples were collected by active method onto a Carbograph 1TD. VOCs were analyzed using the TD-GC-FID/MS method. Presented results include concentrations of identified VOCs and three main group of compounds (aliphatic, aromatic, and cycloalkanes), as well as 18 target compounds and 10 main hydrocarbons, presented in vehicle interiors. It can be stated that interior air composition depended on materials used to finish the interior.
The aim of this study was to identify the potential vaccine antigens in Corynebacterium diphtheriae strains by in silico analysis of the amino acid variation in the 67–72p surface protein that is involved in the colonization and induction of epithelial cell apoptosis in the early stages of infection. The analysis of pili structural proteins involved in bacterial adherence to host cells and related to various types of infections was also performed.A polymerase chain reaction (PCR) was carried out to amplify the genes encoding the 67–72p protein and three pili structural proteins (SpaC, SpaI, SapD) and the products obtained were sequenced. The nucleotide sequences of the particular genes were translated into amino acid sequences, which were then matched among all the tested strains using bioinformatics tools. In the last step, the affinity of the tested proteins to major histocompatibility complex (MHC) classes I and II, and linear B-cell epitopes was analyzed.The variations in the nucleotide sequence of the 67–72p protein and pili structural proteins among C. diphtheriae strains isolated from various infections were noted. A transposition of the insertion sequence within the gene encoding the SpaC pili structural proteins was also detected. In addition, the bioinformatics analyses enabled the identification of epitopes for B-cells and T-cells in the conserved regions of the proteins, thus, demonstrating that these proteins could be used as antigens in the potential vaccine development.The results identified the most conserved regions in all tested proteins that are exposed on the surface of C. diphtheriae cells.
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