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Increase in vasopressin concentration and cardiodepressant activity in the blood dialysates after NMDA and hypertonic saline administration

100%

Goraca A.

Journal of Physiology and Pharmacology

|

1998

|

tom 49

|

nr 4

2

Negative chronotropic effect of endothelin-1 [ET-1] in the cardiac pacemaker tissue

100%

Goraca A.

Journal of Physiology and Pharmacology

|

2003

|

tom 54

|

nr 1

The aim of this study was to asses the direct effect of ET-1 on spontaneous discharge rate of the pacemaker tissue in the presence of isoproterenol. The experiments were performed on pacemaker tissue of the isolated right auricle of the right heart atrium of a two-day-old rat. The spontaneous discharge rate of the pacemaker tissue was recorded on the ECG apparatus and analyzed by the computer. ET-1 alone did not significantly affect the discharge rate of the pacemaker tissue. Isoproterenol rapidly increased the discharge rate of the pacemaker tissue. ET-1 had negative chronotropic effect in the presence of isoproterenol. JKC – 301, a blocker of ETA receptors, significantly reduced the negative chronotropic effect of ET-1 in the presence of isoproterenol, whereas IRL – 1038, a blocker of ETB receptors, did not significantly affect the negative chronotropic effect of ET-1 in the presence of isoproterenol. In conlusion, the negative chronotropic effect of ET-1 in the presence of beta-adrenergic stimulation the pacemaker tissue of the right auricle of the right heart atrium of the two-day-old rat is mediated by ETA receptors.

3

Prophylaxis with alpha-lipoic acid against lipopolysaccharide-induced brain injury in rats

63%

Goraca A., Aslanowicz-Antkowiak K.

Archivum Immunologiae et Therapiae Experimentalis

|

2009

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tom 57

|

nr 2

141-146

4

The lack of influence of some neuropeptides present in the posterior pituitary lobe on the frequency of spontaneous contraction of the isolated heart auricle

63%

Goraca A., Traczyk W. Z.

Acta Physiologica Polonica

|

1990

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tom 41

|

nr 7

Gorąca A., Traczyk W. Z.: The lack of influence of some neuropeptides present in the posterior pituitary lobe on the frequncy of spontaneous contraction of the sisolated heart auricle. Acta Physiol. Pol. 1940, 4 (6): Investigations have shown the presence of a cardiodepressant factor in the fluid incubating the posterior pituitary lobe “in situ”, which decreased contraction frequency of the isolated heart auricle (Acta Physiol. Pol., 1984, 35: 460-468). The influence on the spontaneuos contraction frequency of the isolated heart auricle of the following synthetic neuropeptides was determined: substance P, leu-enkephalin, met-enke- phalin, angiotensin II, arg-vasopressin, oxytocin, delta sleep-inducing peptide and atrial natriuretic factor. It was found that the investigated neuropeptides had no effect on the contraction frequency of the isolated auricle of the heart right atrium of two-day-old rat in a concentration from 2.1 × 10⁻⁷ to 1 × 10⁻³ mol/1 in the bathing medium and it was concluded that their biological properties differ from the cardiodepressant factor.

5

Influence of osmotic stimulation on vasopressin (AVP) and cardiodepressant factor (CDF) release into the blood

63%

Goraca A., Traczyk W. Z.

Acta Neurobiologiae Experimentalis

|

1997

|

tom 57

|

nr 5

6

Increase of cardiodepressant activity in medium incubating the posterior pituitary lobe in situ during vagal nerve stimulation in rat

63%

Goraca A., Traczyk W. Z.

Journal of Physiology and Pharmacology

|

1991

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tom 42

|

nr 3

Previous studies have indicated that there is a cardiodepressant factor in the medium incubating the posterior pituitary lobe in situ. The cardiodepressant activity of the medium incubating the posterior pituitary lobe before and during stimulation of the vagus nerves was tested on isolated auricles of the right heart atrium of a two-day-old rat. It was found that the medium incubating the posterior pituitary lobe collected before stimulation decreased the contraction rate of the auricle by 34%, while that collected during the intermittent stimulation of the central ends of the cut vagus nerves caused a decrease of the auricle contractions frequency by 52%. The addition of cholinergic, serotoninergic, histaminergic receptor blockers or prostaglandin synthetase into Ringer-Lock’s solution bathing the auricle has no effect on the changes of the contraction rate caused by the incubation medium.

7

Appearance of a cardiodepressant factor in the medium incubating in situ the posterior pituitary lobe of the rat II. Diffrentiation from other active compounds

63%

Goraca A., Traczyk W.

Acta Physiologica Polonica

|

1988

|

tom 39

|

nr 2

Gorąca, A. and Traczyk, W. Z.: Appearance of a cardiodepressant factor in the medium incubating in situ the posterior pituitary lohe of the rat. II. Differentiation from other active compounds. Acta physiol, pol., 1988, 39 (2): 98-111. In the previous article the occurrence of cardiodepressant activity in the fluid incubating in situ the posterior pituitary lobe of the rat was described (Acta Physiol. Pol., 1984, 35, 460-468). In the present study the cardiodepressant fraction of the medium incubating in situ the posterior pituitary lobe was subjected to further purification by means of thin-layer chromatography on Silica gel G. Three fractions were isolated, one of which caused a 69% decrease of beating rate of an isolated auricles of the right atrium in the two-day-old rats. In this fraction the presence of neither lipids nor compounds containing phosphate residue was detected. On the basis of the site of elution of the cardiodepressant fraction from the column filled with Sephadex G-15, it was demonstrated that the molecular weight of the cardiodepressant factor was less than 1500 and more than 832. The obtained results show that the cardiodepressant activity is associated with a low-molecule compound, probably with peptide structure, which is released into the fluid incubating in situ the posterior pituitary lobe.

8

Influence of nuclear factor-kappaB inhibition on endothelin-1 induced lung edema and oxidative stress in rats

63%

Piechota A., Goraca A.

Journal of Physiology and Pharmacology

|

2011

|

tom 62

|

nr 2

The aim of the present study is to determine the effects of the BAY 11-7082, a nuclear factor-kappaB (NF-B) inhibitor, on endothelin-1 (ET-1) induced lung edema, the level of reactive oxygen species (ROS) and tumor necrosis factor alpha (TNF-) in the lungs. Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (12.5 µg/kg b.w., i.v.); Group III: BAY 11-7082 (10 mg/kg b.w., i.v.) administered one hour before saline; Group IV: BAY 11-7082 (10 mg/kg b.w., i.v.) administered 1 hour before ET-1 (12.5 µg/kg b.w., i.v.). Injection of ET-1 alone showed a significant (P<0.001) increase in thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) level as well as a decrease (P<0.01) in GSH level and GSH/GSSG ratio (P<0.02). BAY 11-7082 significantly decreased TBARS (P<0.01) and H2O2 (P<0.05) level as well as improved the redox status (P<0.02) in the lungs. BAY 11-7082 also prevented ET-1 induced lung edema (P<0.05). The concentration of TNF- (P<0.02) and p65 subunit of NF-B signaling compound (P<0.001) was increased in the presence of ET-1, while BAY 11-7082 decreased both TNF- level (P<0.05) and p65 subunit concentration (P<0.01). Our results indicate that BAY 11-7082 plays a protective role in ET-1 induced oxidative lung injury. It successfully prevents lung edema as well as ROS and TNF- overproduction. Our results also highlight the important role of the NF-B pathway in ET-1 induced lung injury and ROS overproduction.

9

Cardiodepressing factor appearing in the medium used for in situ incubation of the posterior pituitary lobe in rats

63%

Goraca A., Jodelis-Marcinkiewicz S.

Acta Physiologica Polonica

|

1979

|

tom 30

|

nr 1

10

Protective effects of early treatment with lipoic acid in LPS- induced lung injury in rats

63%

Goraca A., Jozefowicz-Okonkwo G.

Journal of Physiology and Pharmacology

|

2007

|

tom 58

|

nr 3

A lipopolysaccharide (LPS) stimulates the synthesis and releases several metabolites from phagocytes which can lead to an endotoxic shock characterized by multiple organ injury with the earliest to occur in the lungs. Among LPS-induced metabolites, reactive oxygen species are considered to play a crucial pathogenetic role in the lung damage. In this study, the effect of early administration of an antioxidant, alpha-lipoic acid (LA), on pulmonary lipid peroxidation, lung hydrogen peroxide (H202) concentration, and lung sulfhydryl group content was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg, i.v.). In addition, lung edema was assessed with wet-to-dry lung weight (W/D) ratio. Animals were treated intravenously with normal saline or LA 60 mg/kg or 100 mg/kg 30 min after LPS injection. After a 5 h observation, animals were killed and the lungs were isolated for measurements. Injection of LPS alone resulted in the development of shock and oxidative stress, the latter indicated by a significant increase in the lung thiobarbituric acid reacting substances (TBARS) and H202 concentrations, and a decrease in the lung sulfhydryl group content. The increase in the W/D ratio after the LPS challenge indicated the development of lung edema in response to LPS. Administration of LA after the LPS challenge resulted in an increase in the sulfhydryl group content and a decrease in TBARS and H202 concentration in the lungs as compared with the LPS group. An insignificant decrease in the W/D ratio was observed in rats treated with either dose of LA. These results indicate that the LPS-induced oxidative lung injury in endotoxic rats can be attenuated by early treatment with LA. Administration of LA could be a useful adjunct to conventional approach in the management of septic shock.

11

Increase in cardiodepressant factor release from the posterior pituitary lobe after angiotensin II infusion into the internal carotid artery

63%

Goraca A., Traczyk W. Z.

Journal of Physiology and Pharmacology

|

1997

|

tom 48

|

nr 2

12

Partial purification and identification of cardiodepressant factor from the posterior pituitary lobe in rats

63%

Goraca A., Walkowiak B.

Journal of Physiology and Pharmacology

|

2000

|

tom 51

|

nr 1

13

Beneficial effect of alpha-lipoic acid on lipopolysaccharide-induced oxidative stress in bronchoalveolar lavage fluid

63%

Goraca A., Skibska B.

Journal of Physiology and Pharmacology

|

2008

|

tom 59

|

nr 2

Lipopolysaccharide (LPS) from gram-negative bacteria is a major factor that contributes to multiple organ failure including lung injury. Among LPS-induced metabolites, reactive oxygen species are considered to play a crucial pathogenic role in the lung damage. In this study, the effect of early administration of an antioxidant, a-lipoic acid (LA), on bronchoalveoar lavage fluid (BALF) lipid peroxidation, hydrogen peroxide (H2O2), sulphydryl group (-SH) concentration and total protein concentration was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg, i.v.). The animals were treated intravenously with normal saline or LA (60 mg/kg or 100 mg/kg i.v.) 30 min after LPS injection. Five hours after LPS or saline administration, the animals were sacrificed and BALF was obtained for measurements. The results showed that the levels of oxidative markers, thiobarbituric acid reactive substances (TBARS) and H2O2 were increased significantly in BALF, whereas they were decreased significantly on treatment with LA. The concentrations of -SH groups were significantly increased and total protein concentration was insignificantly decreased in the LPS/LA group. There was no difference in oxidative stress reduction between 60 mg/kg and 100 mg/kg doses. These results indicate that early administration of lipoic acid provides protective effects against endotoxin-induced oxidative stress in the lung and supports the idea that alpha-lipoic acid is a free radical scavenger and a potent antioxidant.

14

Effect of alpha-lipoic acid on LPS-induced oxidative stress in the heart

51%

Goraca A., Piechota A., Huk-Kolega H.

Journal of Physiology and Pharmacology

|

2009

|

tom 60

|

nr 1

61-68

Lipopolysaccharide (LPS) is a gram-negative bacterial endotoxin and a major factor that contributes to multiple organ failure, including heart injury. Myocardial dysfunction in septic shock depends on the presence of proinflammatory cytokines and reactive oxygen/nitrogen species. In this study, the effect of early administration of an antioxidant, -lipoic acid (LA) on lipid peroxidation, hydrogen peroxide (H2O2), sulphydryl groups (-SH groups) and total protein concentration and the glutathione redox system was evaluated in the heart homogenates obtained from LPS-induced endotoxic shock rats (Escherichia coli 026:B6, 30 mg/kg, i.v.). The animals were treated intravenously with saline or LA (60 mg/kg or 100 mg/kg i.v.) 30 min after LPS injection. Five hours after LPS, LA or saline administration, the animals were sacrificed and their hearts were isolated for measurements. Injection of LPS alone resulted in the development of shock and oxidative stress that was indicated by a significant increase in thiobarbituric acid reactive substances (TBARS) and H2O2 concentrations, a decrease in concentration of -SH groups and reduced glutathione, and by decrease in glutathione redox ratio reduced glutathione (GSH)/oxidized glutathione (GSSG) in the heart. Administration of LA after the LPS challenge resulted in an increase in the sulfhydryl group content and a decrease in TBARS and H202 concentrations in the heart as compared with the LPS group. In addition, the treatment of LA after LPS challenge significantly decreased the level of GSSG, increased the level of GSH in heart homogenates resulting in an increase of the GSH/GSSG ratio compared with the LPS group. There was no difference in oxidative stress reduction between 60 mg/kg and 100 mg/kg doses. These results indicate that early administration of LA is highly effective in dampening endotoxin-induced oxidative stress in the heart and in improving the glutathione redox system. This study supports the idea that -LA is a free radical scavenger and a potent antioxidant.

15

Appearance of a cardiodepepressant factor in the medium incubating in situ the posterior pituitary lobe of the rat

51%

Goraca A., Tranda T., Traczyk W. Z.

Acta Physiologica Polonica

|

1984

|

tom 35

|

nr 5-6

16

The NADPH oxidase family and its inhibitors

51%

Kleniewska P., Piechota A., Skibska B., Goraca A.

Archivum Immunologiae et Therapiae Experimentalis

|

2012

|

tom 60

|

nr 4

17

Effects of extremely low frequency magnetic field on oxidative balance in brain of rats

51%

Ciejka E., Kleniewska P., Skibska B., Goraca A.

Journal of Physiology and Pharmacology

|

2011

|

tom 62

|

nr 6

18

Effects of extremely low frequency magnetic field on the parameters of oxidative stress in heart

51%

Goraca A., Ciejka E., Piechota A.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 3

333-338

Increasing production of free radicals in organisms is one of the putative mechanisms by which a extremely low frequency magnetic field (ELF-MF) may affect biological systems. The present study was designated to assess if ELF-MF applied in the magnetotherapy, affects generation of reactive oxygen species (ROS) in heart tissue and antioxidant capacity of plasma according to its working time. The experiments were performed on 3 groups of animals: group I - control; group II - exposed to 40 Hz, 7 mT, 30 min/day for 14 days (this field is commonly applied in magnetotherapy); group III - exposed to 40 Hz, 7 mT, 60 min/day for 14 days. Control rats were housed in a separate room without exposure to ELF-MF. Immediately after the last exposure, blood was taken from the tail vein and hearts were removed under anesthesia. The effect of the exposure to ELF-MF on oxidative stress was assessed on the basis of the measurements of thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), total free sulphydryl groups (-SH groups) and reduced glutathione (GSH) concentrations in heart homogenates. The total antioxidant capacity of plasma was measured using ferric reducing ability method (FRAP). Exposure to ELF-MF (40 Hz, 7 mT, 30 min/day for 2 weeks) did not significantly alter tissue TBARS, H2O2, total free -SH groups, reduced glutathione (GSH) and total antioxidant capacity of plasma. By contrast, ELF-MF with the same frequency and induction but used for 60 min/day for 14 days caused significant increase in TBARS and H2O2 concentration (P<0.01) and decrease in the concentration of GSH (P<0.05) and total free -SH groups in heart homogenates. Moreover, exposure of rats to ELF-MF (40 Hz, 7 mT, 60 min/day for 2 weeks) resulted in the decrease of plasma antioxidant capacity. Our results indicate that effects of ELF-MF on ROS generation in the heart tissue and antioxidant capacity of plasma depend on its working time.

19

The influenceof amino acids, vasopressin and oxytocin on spontaneous contraction of the right auricle of the right atrium of two-day-old rats in vitro

51%

Goraca A., Traczyk W. Z., Konarzewska A.

Acta Physiologica Polonica

|

1984

|

tom 35

|

nr 5-6

20

The increase in the cardiodepressant activity and vasopressin concentration in the sella turcica venous blood during vagal afferents stimulation or after angiotensin II infusion

51%

Goraca A., Orlowska-Majdak M., Traczyk W. Z.

Journal of Physiology and Pharmacology

|

1996

|

tom 47

|

nr 2

Ograniczanie wyników

Increase in vasopressin concentration and cardiodepressant activity in the blood dialysates after NMDA and hypertonic saline administration

Negative chronotropic effect of endothelin-1 [ET-1] in the cardiac pacemaker tissue

Prophylaxis with alpha-lipoic acid against lipopolysaccharide-induced brain injury in rats

The lack of influence of some neuropeptides present in the posterior pituitary lobe on the frequency of spontaneous contraction of the isolated heart auricle

Influence of osmotic stimulation on vasopressin (AVP) and cardiodepressant factor (CDF) release into the blood

Increase of cardiodepressant activity in medium incubating the posterior pituitary lobe in situ during vagal nerve stimulation in rat

Appearance of a cardiodepressant factor in the medium incubating in situ the posterior pituitary lobe of the rat II. Diffrentiation from other active compounds

Influence of nuclear factor-kappaB inhibition on endothelin-1 induced lung edema and oxidative stress in rats

Cardiodepressing factor appearing in the medium used for in situ incubation of the posterior pituitary lobe in rats

Protective effects of early treatment with lipoic acid in LPS- induced lung injury in rats

Increase in cardiodepressant factor release from the posterior pituitary lobe after angiotensin II infusion into the internal carotid artery

Partial purification and identification of cardiodepressant factor from the posterior pituitary lobe in rats

Beneficial effect of alpha-lipoic acid on lipopolysaccharide-induced oxidative stress in bronchoalveolar lavage fluid

Effect of alpha-lipoic acid on LPS-induced oxidative stress in the heart

Appearance of a cardiodepepressant factor in the medium incubating in situ the posterior pituitary lobe of the rat

The NADPH oxidase family and its inhibitors

Effects of extremely low frequency magnetic field on oxidative balance in brain of rats

Effects of extremely low frequency magnetic field on the parameters of oxidative stress in heart

The influenceof amino acids, vasopressin and oxytocin on spontaneous contraction of the right auricle of the right atrium of two-day-old rats in vitro

The increase in the cardiodepressant activity and vasopressin concentration in the sella turcica venous blood during vagal afferents stimulation or after angiotensin II infusion