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After the intramuscular administration of Engemycin L.A. inj. ad us. vet. (0.8 ml. 10kg⁻¹) to seven ewes for four consecutive days, the milk samples were collected at every 12 h of the experiment, from 0 to 166 h. The milk samples were analysed by the high performance liquid chromatography (HPLC) method. Twelve hours after the beginning of the experiment, all milk samples were positive for the presence of oxytetracycline (OTC) residues. The maximum mean concentrations were reached 12 h after the final administration (3.67 μg.g⁻¹). The OTC residues were not detected in any milk sample 84 h after the final administration.
The aim of the studies was the evaluation of relationship between the tetracycline residue levels in milk of cows treated intrauterinary with different doses of tetracycline and clinical form of endometritis. Six cows were treated by intrauterine route with pessaries containing tetracycline hydrochloride. The cows in group 1 (n = 3) were affected with acute endometritis, and the cows in group 2 (n = 3) by chronic endometritis. The animals were treated with Tetramykoin supp. uter. ad us. vet. (Léčiva, Praha, Czech Republic) at a dosage of 3g/cow (group 1), and 2g/cow (group 2). Milk samples were collected before treatment and at 12-h intervals for 144 h. The samples were analysed by the high-performance liquid chromatography method with solid phase extraction. The mean concentrations of tetracycline in milk 12 h after treatment were 227.7 μg.1⁻¹ in both groups. The tetracycline residues reached their maximum mean concentrations 24 h after the treatment (276.4 μg.1⁻¹, and 369.6 μg.1⁻¹, for group 1 and 2 resp.). Residues of 13.7 μg.1⁻¹ were detected in group 2 at 108 h. At 120 h the mean concentrations were 33.1μ g.1⁻¹ for group 1 and zero for group 2. No residues were detected after 144 h.
Blood serum concentrations and excretion of oxytetracycline (OTC) into milk after the i.m. administration of long-acting preparations in adult ewes were studied. Four ewes were treated with L.A. Engemycin and four - with L.A. Tetravet. OTC was administered to animals in a single dose of 20 mg/kg of live weight. OTC concentrations in blood serum were determined 1, 6 and 24 hours and then 2 to 8 days after the treatment. The milk samples were collected from the morning and evening milking up to 166 hours. OTC was determined by the HPLC method with solid phase extraction. Significantly higher serum concentrations of OTC (p < 0.05) were recorded after Tetravet administration from the 24th hour to the 5th day. OTC was detected in blood serum up to the 5th day after Engemycin administration, and up to the 6th day after Tetravet administration. The OTC residues in milk reached their maximum mean concentrations after 24 hours (2.525, and 2.186 mg/I, respectively). Then the mean OTC concentrations rapidly decreased and the residues were below the maximum residual limit (0.1 mg/l) 96 hours after the administration. OTC residues were detected in the first group up to 144 hours (0.021 mg/l), and up to 168 hours (0.031 mg/I) in the second group. The results indicated on the influence of the vehicle (polyvinylpyrrolidone in Engemycin and dimethylacetamide in Tetravet) on the serum kinetics and time of excretion of OTC into milk of treated ewes.
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