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1

Screening of probiotic goat milk tablets using plackett - burman design

100%
Chen H., Zhanag J., Shu G.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2014
|
tom 13
|
nr 4
Background. Probiotics defined as additional microorganisms were added to goat milk powder, which not only improves the intestinal flora balance but also promotes human and animal health. The objectives of this study were to improve and guarantee high probiotics viable count and accordance with consumer’s acceptance. Material and methods. The reading selected the number of colony between 30 and 300, then calculated the viable count per gram of goat milk tablet (cfu/g). The items of sensory evaluation included: appearance, flavour, colour, texture and taste. The score test was composed of 5 trained assessors, scored combination of different formulations (fuli marks of 100 points) and recorded the results. Results. Analysis of the results showed that sucrose, inulin and mannitol were selected as the main effective parameters on both viable count and sensory evaluation. Furthermore optimization of the formulation of probiotic goat milk tablets was to maximise the probiotics viable count to achieve 9.5·108 cfu/g and its scores of sensory evaluation to get 94 points. Discussion. Future probiotics products will be combined with a variety of probiotics, which can display their respective advantages and characteristics. Thus the products will not only be in accordance with the requirements of human health and trend of social development, but also will quickly become a favorite among consumers.
2

Application of the Plackett-Burman design to determine the main factors affecting the anti-oxidative activity of goat's milk casein hydrolyzed by Alcalase and papain

88%
Shu G., Mei S., Zhang Q., Xin N., Chen H.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2018
|
tom 17
|
nr 3
3

Effect of incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder on ace inhibitory activity in fermented milk by L. Plantarum LP69

88%
Shu G., Yang H., Chen H., Zhang Q., Tian Y.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2015
|
tom 14
|
nr 2
Background. Angiotensin I converting enzyme (ACE) plays an important physiological role in regulating hypertension. Lactic acid bacteria are known to produce ACE inhibitory peptides which can lower hypertension during fermentation. Methods. The effect of incubation time (0~36 h), inoculum size (3,4, 5, 6 and 7%, v/v), temperature (25, 30, 35, 40 and 45°C), sterilization time (5, 10, 15, 20 and 25 min), concentration of goat milk powder (8, 10, 12, 14 and 16%, w/v) and whey powder (0.5, 0.6, 0.7, 0.8 and 0.9%, w/v) on ACE inhibitory peptides fermented from goat milk by Lactobacillus plantarum LP69 was investigated using single factor experiment. Results. The optimal incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder in fermented milk by L. plantarum LP69 was 14 h, 3.0%, 35°C, 20 min, 14% and 0.70% for ACE inhibitory activity and 22 h, 3.0%, 40°C, 25 min, 16% and 0.60% for viable cell counts, respectively. Conclusion. The incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder had a significant influence on ACE inhibitory activity in fermented milk by Lactobacillus plantarum LP69, the results are beneficial for further screening of main factors by using fractional factorial designs.
4

Fermentation optimization of goat milk wit Lactobacillus acidophilu and Bifidobacterium bifidum by Box-Behnken design

88%
Shu G., Bao C., Chen H., Wang C., Yang H.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2016
|
tom 15
|
nr 2
5

Effects of sugar alcohol and proteins on the survival of Lactobacillus bulgaricus LB6 during freeze drying

88%
Chen H., Chen S., Chen H., Wu Y., Shu G.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2015
|
tom 14
|
nr 2
Background. Lactobacillus bulgaricus LB6 is a bacterium which was selected in the commercial yoghurt with high angiotensin converting enzyme (ACE) inhibitory activity. Preparation of concentrated starter cultures via freeze drying is of practical importance to dairy and food industries. Material and methods. We optimized the optimal sugar alcohol and proteins for Lactobacillus bulgaricus LB6 during the process of freeze drying using a Plackett-Burman design. In our initial tests survival rate and the number of viable cells were associated with the type of ly oprotectant used and so our optimization protocol focused on increasing survival rate. Substances that had previously had a protective effect during freeze drying were investigated, for example: mannitol, sorbitol, xylitol, meso-erythritol, lactitol, whey protein isolate 90, bovine serum albumin, and whey protein concentrate 80 and soy protein isolate 70. Results. We found that the optimum sugar alcohol and proteins for survival of Lactobacillus bulgaricus LB6 were whey protein concentrate (p = 0.0040 for survival rate), xylitol (p = 0.0067 for survival rate) and sorbitol (p = 0.0073 for survival rate), they showed positive effect (whey protein concentrate and sorbitol) or negative effect (xylitol). Discussion. The effectiveness of three chosen sugar alcohols and protein implied that they could be used as lyoprotectant for Lactobacillus bulgaricus LB6 in the further research, the optimal composition of sugar alcohol and protein for the lyoprotectant use must be established.
6

Optimization of the medium for Lactobacillus acidophilus by Plackett-Burman and steepest ascent experiment

76%
Chen H., Niu J., Qin T., Ma Q., Wang L., Shu G.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2015
|
tom 14
|
nr 3
Background. Lactobacillus acidophilus not only improves the intestinal flora balance but also inhabits the growth of undesirable microorganisms in intestine, which is benefit to the health of humans and animals. Plackett-Burman and steepest ascent experiment are the rapid and concise ways of screening the main effective factors. This study is aimed to select the main influence factors and optimize the medium for Lactobacillus acidophilus by Plackett-Burman experiment and steepest ascent experiment. Material and methods. The ideal carbon source was screened among glucose, maltose, lactose and whey powder, and the ideal nitrogen source was screened among casein hydrolysate, peptone, yeast extract powder, fish meal, carbamide, ammonium sulfate and sodium nitrate by single factor experiment. Plackett-Burman and steepest ascent experiment were applied to screen the main effective factors of Lactobacillus acidophilus among peptone, beef extract, yeast extract powder, glucose, K2HP04, C6H1407N2, CH3COONa, MgS04 and Tween-80. Result. The results indicated that glucose (p = 0.01510) as negative factor and K,HP04 ip = 0.02017) as positive effect were the significant growth factors of Lactobacillus acidophilus, CH3COONa (p = 0.09273) as positive effect was an important factor, and the optimized medium was as follows: glucose - 21 g/L, K2HP04 - 3.5 g/L, CH3COONa - 6.5 g/L, peptone - 10 g/L, beef extract - 8 g/L, yeast extract powder - 8 g/L, C6H1407N2 - 2 g/L, MgS04 - 0.2 g/L and Tween-80 - 1 mL/L when the maximum viable count could achieve 2.72* 109 cfii/mL. Discussion. The experimental model is reliable and the experimental results are of good stability. Variance analysis is performed to determine the adequacy and significance of the linear model. Thus, Plackett-Burman and steepest ascent experiment improve the veracity of optimization the medium for Lactobacillus acidophilus compared with the previous research.
7

Effect of complexation conditions on microcapsulation of Lactobacillus acidophilus in xanthan-chitosan polyelectrolyte complex gels

76%
Chen H., Song Y., Liu N., Wan H., Shu G., Liao N.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2015
|
tom 14
|
nr 3
Background. Lactobacillus acidophilus has become increasingly popular because of their beneficial effects on health of their host, and are called proboscis. In order to exert beneficial effects for probiotics, they must be able to tolerate the acidic conditions of the stomach environment and the bile in the small intestine. Micro- encapsulated form has received reasonable attention, since it can protect probiotic organisms against an unfavourable environment, and to allow their release in a viable and metabolically active state in the intestine. The aim of this study was to investigate some factores, such as chitosan solution pH and concentration, xanthan concentration, cell suspension-xanthan ratio, mixed bacteria glue liquid-chitosan ratio, which impacted the process of microencapsulation of L. acidophilus. Material and methods. In this study, L. acidophilus was immobilized with xanthanthitosan gel using extrusion method. The viable counts and encapsulation yield of L. acidophilus encapsulated in different chitosan solution pH (4.5, 5, 5.5 and 6), in different chitosan concentration (0.5%, 0.7%, 0.9% and 1.1%), in different xanthan concentration (0.5%, 0.7%, 0.9% and 1.1%), in different cell suspension-xanthan ratios (1:5, 1:10, 1:15 and 1:20), in different mixed bacteria glue liquid-chitosan ratios (1:3,1:4,1:5 and 1:6), have been investigated by single factor experiment method. Results. The optimum conditions of microencapsulated L. acidophilus have been observed. The optimum chitosan solution pH for L. acidophilus was 5.5; the optimum chitosan concentration was 0.9%; the optimum xanthan concentration was 0.7%; the optimum cell suspension-xanthan ratio was 1:10; the optimum mixed bacteria glue liquid-chitosan ratio was 1:3. Conclusions. These results will be helpful to further optimize the process of L. acidophilus microencapsulation, and provide reference for obtaining higher viable counts and entrapped yield of L. acidophilus microcapsules.
8

Effect of bacteria proportion on the fermentation of goat yoghurt with probiotic culture

76%
Shu G., Wang S., Chen Z., Chen H., Wang C., Ma Y.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2015
|
tom 14
|
nr 4
9

Effects of glycyl-glutamine on the growth performance and lipid deposition in Yue-Huang broilers

64%
Shu G., Gao P., Zhu X. T., Jiang Q. Y., Xu P. W., Zhou G. X., Sun F. W., Fu W. L.
Journal of Animal and Feed Sciences
|
2007
|
tom 16
|
nr 2
232-240
10

Effect of maternal dietary supplementation with phytosterol esters on muscle development of broiler offspring

52%
Wang L., Zuo X., Zhao W., Zhou G., Luo L., Yang K., Shu G., Wang S., Gao P., Zhu X., Jiang Q., Wang L.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 1
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