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Background: Due to its deep penetration into the dermis, ultraviolet A (UVA) radiation is considered a primary factor in skin photoageing. The aim of this study is to use a qualitative and quantitative analysis to determine the structural parameters of skin photoageing in mice exposed to UVA radiation, with or without the application of a photoprotective cream. Materials and methods: The experiment consisted of the radiation of female BALBc mice in a solarium by UVA rays, up to total dosages of 7800 J/cm² and 12500 J/cm². A total of 78 animals were divided into four experimental and two control groups. All animals were shaved and the animals in two experimental groups were treated with a photoprotective cream half an hour before exposure. The samples of the treated skin were stained with haematoxylin-eosin and Van-Gieson staining methods. All measurements, except for the presence of dyskeratosis, were taken using ImageJ 150i software. Results: In the study, the signs of skin photoageing were more evident in untreated groups of animals. Dyskeratosis was more frequent in both of the untreated groups of animals (p = 0.004 and p = 0.003). The lowest values of epidermal thickness (13.8 ± 2.6 μm and 12.7 ± 2.3 μm) were present in both of the untreated groups of animals (p < 0.001 and p < 0.001). The highest values of stratum corneum thickness (34.3 ± 8.5 μm) were observed in the untreated, shorter radiated group of animals (p < 0.001) which was irradiated for the shortest period of time. Beside the control groups, the highest length of dermo-epidermal junction was recorded in the group of treated, longer radiated animals (1467.6 ± 94.6 μm; p = 0.373). The lowest values of dermal thickness (115.9 ± 10.5 μm and 134.8 ± 21.8 μm) and volumetric density of the collagen fibres (31.92 ± 3.19% and 29.40 ± 4.54%) were present in both untreated groups of animals (p < 0.001, p < 0.001, p = 0.035). Conclusions: Skin photoageing was most pronounced in the groups of animals irradiated without the application of photoprotective cream. (Folia Morphol 2020; 79, 3: 548–556)
Background: The most reliable data about arterial variations, which are very important in surgery and radiology, can be obtained from a large series of patients. Materials and methods: We examined angiographic and multislice computerised tomography (MSCT) images in a group of 1,265 patients and in 1 dissected specimen. Results: While in 946 (74.72%) of the patients a normal vascular pattern (type I) was noticed, in the remaining 320 (25.28%) patients variations of the branches of the aortic arch were found, which were classified into types II through VIII and a few subtypes. Type II (2.84%) comprised a common origin of the left common carotid and subclavian arteries. Type III (15.56%) was related to an origin of the left subclavian artery from the brachiocephalic trunk. Type IV (0.55%) included the aortic origin of both common carotid and subclavian arteries, with the right subclavian artery having a retroesophageal course. Type V (0.24%) included the same 4 supra-aortic branches, which, however, arose from a double or a right- -sided aortic arch. Type VI (3.63%) comprised the aortic origin of the left vertebral artery, type VII (0.24%) the same origin of the right vertebral artery, and type VIII (2.22%) the aortic origin of the thyroidea ima artery. A corresponding embryological background and clinical implications of the described aberrant vessels were presented. Conclusions: In more than one quarter of the cases, the branching pattern of the examined arteries did not follow the classical pattern. Detailed knowledge of aortic branch variations is of great significance in anatomy, embryology, and clinical medicine, especially in radiology and thoracic surgery. (Folia Morphol 2013; 72, 2:113–122)
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