Wyniki wyszukiwania

1

Indukcja biosyntezy glukotropeoliny i aktywności myrozynazy w korzeniach włośnikowych nasturcji

100%

Wielanek M., Urbanek H.

Biotechnologia

|

2000

|

nr 4

2

Glucotropaeolin contents and myrosinase activities in leaves of intact plants, callus and hairy root cultures of Tropaeolum majus L.

100%

Wielanek M., Urbanek H.

Biological Bulletin of Poznań. Supplement

|

1997

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tom 34

3

Effects of the ATH-rich extract on lead absorption and localization in Allium cepa L. roots

81%

Glinska S., Gabara B., Wielanek M., Sklodowska M.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

4

Hairy root cultures of Rehmannia glutinosa and production of iridoid and phenylethanoid glycosides

81%

Piatczak E., Krolicka A., Wielanek M., Wysokinska H.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 6

Hairy root lines through the infection of Agrobacterium rhizogenes strain (A4) were established from shoot tips and leaves of Rehmannia glutinosa Libosch. Ten lines of hairy roots were selected on the basis of biomass increase in half-strength Gamborg medium (½ B5). Transgenic status of the roots was confirmed by polymerase chain reaction using rolB and rolC specific primers. Iridoid glycosides (catalposide, loganin, aucubin and catalpol) and phenylethanoid glycosides (verbascoside and isoverbascoside) identified using HPLC–ESI–MS, and their contents were compared with untransformed root culture and roots of 1-year-old field-grown plants of R. glutinosa by RP-HPLC. The growth and production of secondary metabolites in ten hairy root lines varied considerably as to the media. Woody plant (WP) medium displayed higher growth in terms of fresh (FW) and dry weights (DW) compared to ½ B5 medium. High-yielding hairy root lines produced higher amounts of loganin, catalposide, verbascoside and isoverbascoside in comparison to the untransformed root culture and roots of 1-year-old field-grown plants. The highest amounts of catalposide and loganin in transformed roots were 4.45 mg g⁻¹ DW (RS-2 hairy root line) and 4.66 mg g⁻¹ DW (RS-1 hairy root line), respectively. Aucubin and catalpol were detected in some lines in trace amounts. The highest amounts of verbascoside (16.9 mg g⁻¹ DW) and isoverbascoside (3.46 mg g⁻¹ DW) were achieved in RS-2 root line. The contents of catalposide, verbascoside and isoverbascoside in high-producing lines were several times higher than in untransformed root culture and roots of R. glutinosa plants grown in soil. Loganin and aucubin could not be detected in roots of fieldgrown plants. However, the levels of catalpol were much lower in the in vitro roots.

5

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The influence of selenium on antifungal activity of secondary metabolites of transformed root cultures of Tropaeolum majus, Schkuhria pinnata and Saussurea nepalensis

81%

Bogusz D., Janas J., Wielanek M., Sklodowska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

6

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato [Lycopersicon esculentum Mill.] protoplast yield

81%

Kuzniak E., Wielanek M., Malolepsza U., Urbanek H.

Acta Agrobotanica

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1995

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tom 48

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nr 2

113-120

The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x l0(7) /g FW was obtained from leaves of in vitro grown plants. Low light intensity during donor plants in vitro culture and dark pretreatment were essential for successful protoplast isolation while cold pretreatment was not. Tissue preplasmolysis prior to transfer to enzyme mixture increased 4-fold the number of isolated protoplasts. Glycine and bovine serum albumin in the isolation medium did not significantly influence the protoplast yield.

7

Biotransformation of hydroquinone to arbutin by suspension cultures of Cynara scolymus L.

81%

Wielanek M., Parada D., Bergier K., Sklodowska M.

Herba Polonica. Suplement

|

2005

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tom 51

|

nr 1

8

Aktywność enzymów glutationozależnych w procesie aklimatyzacji roślin do stresu solnego badania in vitro i in vivo

81%

Naliwajski M. R., Drozdz K., Wielanek M., Sklodowska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

|

tom 524

Tolerancja na stres solny związana jest między innymi ze sprawną obroną antyoksydacyjną. Zmiany aktywności enzymów glutationozależnych (GPX i GST) ogórka traktowanych 30 mM NaCl badano w dwóch układach: in vitro i in vivo aklimatyzowanych i nieaklimatyzowanych do stresu. Proces aklimatyzacji roślin ogórka i zawiesiny komórkowej 3 mM NaCl spowodował istotny (p < 0,05) wzrost aktywności peroksydazy glutationowej o blisko 90-100% w porównaniu z odpowiednimi układami nieaklimatyzowanymi do stresu solnego. Wystąpienie zasolenia (30 mM NaCl) wpłynęło istotnie (p < 0,05) na wzrost aktywności GPX o odpowiednio o 116%, 113%, 48% i 59% po 1, 3, 24 i 72 godzinach od wystąpienia stresu w stosunku do wartości kontrolnych. Aktywność peroksydazy glutationowej w liściach roślin nieaklimatyzowanych wzrosła o 42% (p < 0,05) po 24 godz. i o 99% (p < 0,01) po 72 godz. od zastosowania 30 mM NaCl. W hodowli komórkowej aklimatyzowanej do stresu, aktywność GST wykazywała istotny 20 % (p < 0,05) wzrost w odpowiedzi na zasolenie. Obecność stresu solnego spowodowała wzrost aktywności S-transferazy glutationowej w roślinach nie poddanych aklimatyzacji po 24 godz. o 111% (p < 0,01) oraz odpowiednio o 188% (p < 0,001) i 100% (p < 0,01) po 24 i 72 godz. od wystąpienia czynnika stresowego w liściach roślin aklimatyzowanych. Prezentowane wyniki dowodzą, że w procesie aklimatyzacji w obu badanych układach kluczowe znaczenie wydaje się odgrywać peroksydaza glutationowa.

9

Nickel-induced depression of nitogen assimilation in wheat roots

81%

Gajewska E., Wielanek M., Bergier K., Sklodowska M.

Acta Physiologiae Plantarum

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2009

|

tom 31

|

nr 6

The influence of 50 and 100 µM Ni on the activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) was studied in the wheat roots. Root fresh weight, tissue Ni, nitrate, ammonium, glutamate and protein concentrations were also determined. Exposure to Ni resulted in a marked reduction in fresh weight of the roots accompanied by a rapid accumulation of Ni in these organs. Both nitrate and ammonium contents in the root tissue were considerably enhanced by Ni stress. While protein content was not significantly influenced by Ni application, glutamate concentration was slightly reduced on the first day after treatment with the higher Ni dose. Treatment of the wheat seedlings with 100 µM Ni led to a decrease in NR activity; however, it did not alter the activation state of this enzyme. Decline in NiR activity observed after application of 100 µM Ni was more pronounced than that in NR. The activities of GS and NADH-GOGAT also showed substantial decreases in response to Ni stress with the latter being more susceptible to this metal. Starting from the fourth day, both aminating and deaminating GDH activities in the roots of the seedlings supplemented with Ni were lower in comparison to the control. While the activity of AspAT remained unaltered after Ni application that of AlaAT showed a considerable enhancement. The results indicate that exposure of the wheat seedlings to Ni resulted in a general depression of nitrogen assimilation in the roots. Increase in the glutamate-producing activity of AlaAT may suggest its involvement in supplying the wheat roots with this amino acid under Ni stress.

10

The influence of BTH and arbutin on PAL activity and phenolic compounds content in cucumber leaves infected with Pseudomonas syringae pv. lachrymans

81%

Sklodowska M., Wielanek M., Gajewska E., Kuzniak E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

11

Production of potentially healthy seleno-glucosinolates in hairy root cultures of Barbarea verna (Mill.) Ascherson

81%

Wielanek M., Bergier K., Sklodowska M.

Herba Polonica. Suplement

|

2005

|

tom 51

|

nr 1

12

Regeneracja Oxalis triangularis z korzeni transformowanych i wplyw glutationu na wytworzanie antocyjan

81%

Wielanek M., Urbanek H., Dobras M.

Biotechnologia

|

2004

|

nr 2

260-266

13

Zdolność Phalaris arundinacea L. do fitoremediacji

81%

Majorowicz H., Urbanek H., Wielanek M., Zalewski M.

Biotechnologia

|

2003

|

nr 4

81%

Parada D., Wielanek M., Kuzniak E., Sklodowska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2008

|

tom 524

Recent studies revealed that reactive oxygen species, antioxidants and related redox signals are key elements in the regulation of plant defense responses, including the synthesis of secondary metabolites. In this work the potential of artichoke (Cynara scolymus L.) cell suspensions to produce cynarin was studied in relation to the antioxidant profile of the cultures. There were determined the total antioxidant capacity and concentrations of H₂O₂, ascorbate and total phenolics, as well as activities of ascorbate and guaiacol peroxidases, ascorbate oxidase and catalase at different stages of the culture growth cycle in relation to the production of cynarin. Suspension cultures revealed a growth-linked capacity to produce cynarin as identified by qualitative HPLC analysis. The cynarin accumulation kinetics during the exponential phase of growth correlated with the total antioxidant capacity of water-soluble antioxidants whereas the profiles of total phenols and total antioxidant capacity of water-insoluble antioxidants differed. The lowest ascorbate-related antioxidant capacity coincided with the maximum cynarin accumulation in the early stationary phase. The regulation of growth-related prooxidant/antioxidant balance in the artichoke suspension cultures involved the peroxidase/phenolics/ascorbate system scavenging H₂O₂, further affected by ascorbate- and H₂O₂-metabolizing enzymes. These results may be of interest in biotechnological strategies aimed at optimizing the production of secondary metabolites in plant cultures in vitro.

20

Incorporation of inorganic selenium into seleno-glucosinolates in hairy root cultures of Nasturtium officinale R.Br.

81%

Wielanek M., Bergier K., Andrzejewska R., Sklodowska M.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

Ograniczanie wyników

Indukcja biosyntezy glukotropeoliny i aktywności myrozynazy w korzeniach włośnikowych nasturcji

Glucotropaeolin contents and myrosinase activities in leaves of intact plants, callus and hairy root cultures of Tropaeolum majus L.

Effects of the ATH-rich extract on lead absorption and localization in Allium cepa L. roots

Hairy root cultures of Rehmannia glutinosa and production of iridoid and phenylethanoid glycosides

The influence of selenium on antifungal activity of secondary metabolites of transformed root cultures of Tropaeolum majus, Schkuhria pinnata and Saussurea nepalensis

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato [Lycopersicon esculentum Mill.] protoplast yield

Biotransformation of hydroquinone to arbutin by suspension cultures of Cynara scolymus L.

Aktywność enzymów glutationozależnych w procesie aklimatyzacji roślin do stresu solnego badania in vitro i in vivo

Nickel-induced depression of nitogen assimilation in wheat roots

The influence of BTH and arbutin on PAL activity and phenolic compounds content in cucumber leaves infected with Pseudomonas syringae pv. lachrymans

Production of potentially healthy seleno-glucosinolates in hairy root cultures of Barbarea verna (Mill.) Ascherson

Regeneracja Oxalis triangularis z korzeni transformowanych i wplyw glutationu na wytworzanie antocyjan

Zdolność Phalaris arundinacea L. do fitoremediacji

Micropropagation of Physalis ixocarpa L.

Nickel-induced changes in peroxidase activity and phenol concentration in cucumber leaves

Antioxidative enzymes in cucumber cell suspension cultures acclimatized to salt stress - in vitro study

The potential to produce cynarin in Cynara scolymus suspension cultures in relation to their antioxidant profile

The influence of selenium on antifungal activity of extracts of Codonopsis pilosula hairy roots

The potential of producing cynarin in Cynara scolymus L. suspension cultures in relation to their antioxidant profile

Incorporation of inorganic selenium into seleno-glucosinolates in hairy root cultures of Nasturtium officinale R.Br.