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1

Molecular detection and comparison of Gaeumannomyces graminis var. tritici isolates originating from wheat and rye

100%
Irzykowska L.
Journal of Plant Protection Research
|
2007
|
tom 47
|
nr 3
299-308
Gaeumannomyces graminis is an etiologic agent of take-all, economically important disease of cereals worldwide. A polymerase chain reaction with variety-specific primers was successfully used for detection of G. graminis var. tritici in plant tissue. Obtained results showed that this diagnostic method is a very sensitive and useful tool for detection of the pathogen even before disease symptoms arise. DNA polymorphism revealed by RAPD-PCR with three arbitrary primers was suitable for assessing genetic variation among Ggt isolates originating from wheat and rye.
2
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Markery molekularne w diagnostyce chorob podstawy zdzbla i korzeni zboz

100%
Irzykowska L.
Postępy Nauk Rolniczych
|
2006
|
tom 53
|
nr 6
31-40
3

Molecular identification of mating type genes in asexually reproducing fusarium oxysporum and F. culmorum

63%
Irzykowska L., Kosiada
Journal of Plant Protection Research
|
2011
|
tom 51
|
nr 4
Sexually (homothallic and heterothallic) and asexually reproducing species belong to the Fusarium genus. So far, there is no known sexual stage of the F. oxysporum Schlechtend.: Fr. and F. culmorum (W.G. Smith) Sacc. Knowing the reproduction mode is important for the design of successful control strategies, since they are different for clonally and sexually reproducing organisms. In examined sets of asexual F. oxysporum and F. culmorum isolates, the DNA sequences of mating type genes (idiomorphs MAT-1 and MAT-2) were identified. MAT-1 sequence was detected for 33 and 40% of F. oxysporum and F. culmorum isolates, respectively. For the remaining isolates a sequence specific for MAT-2 was amplified.
4

The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil

63%
Werner M., Irzykowska L.
Phytopathologia Polonica
|
2007
|
tom 46
5

Genetic polymorphism of Fusarium culmorum isolates originating from roots and stem bases of barley

63%
Irzykowska L., Baturo A.
Journal of Plant Protection Research
|
2008
|
tom 48
|
nr 3
303-312
Fusarium culmorum is an etiologic agent of barley foot rot. The identification and variability evaluation of F. culmorum isolates, originating from roots and stem bases of spring barley, was carried out using molecular methods. Species-specific SCAR primers were successfully applied to identify F. culmorum isolates from northern and south-eastern Poland. To determine DNA polymorphism on intraspecies level RAPD technique was used. Twenty three RAPD markers revealed DNA polymorphism suitable to assess genetic variation among isolates examined. Cluster analysis of RAPD data identified a few groups of isolates. In some cases grouping of isolates was correlated with their geographic origin.
6
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Occurrence of pathogenic and endophytic fungi and their influence on quality of medicinal plants applied in management of neurological diseases and mental disorders

63%
Wielgusz K., Irzykowska L.
Herba Polonica
|
2017
|
tom 63
|
nr 4
Due to increasing demand of medicinal plants (MPs), quality and safety more attention to the plant health should be paid. Among herb pathogens, especially fungi cause serious diseases in these plants decreasing yield and quality of herbal raw material. Some species, i.e. Fusarium sp., Alternaria sp., Penicillium sp. are known as mycotoxin producers. Paradoxically, self-treatment with herbal raw material can expose the patient to mycotoxin activity. In tissues of some MPs species, asymptomatically endophytic fungi residue. It is known that they are able to influence a biosynthesis of secondary metabolites in their host plant or produce biologically active compounds. Until recently these microorganisms have been neglected as a component of MPs, the reason why there have unexplored bioactivity and biodiversity. The paper presents an overview of herbal plants that are used in the treatment of nervous system diseases. Pathogenic fungi that infect these plants are described. It focused mainly on species producing harmful mycotoxins. The publication presents a list of these mycotoxins and a brief description of their effects on human health. The second part of this article provides information on the occurrence of endophytic fungi in herbal plants and their effects on human health. Coexistence of fungi and medicinal plants is not fully understood but can be crucial to ensure health and safety of patients with neurological diseases and mental disorders.
7

Evaluation of the health of seeds and sprouts intended for human consumption

63%
Irzykowska L., Kononowicz M.
Progress in Plant Protection
|
2017
|
tom 57
|
nr 4
8

Interval mapping of QTLs controlling yield-related traits and seed protein content in Pisum sativum

63%
Irzykowska L., Wolko B.
Journal of Applied Genetics
|
2004
|
tom 45
|
nr 3
9
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Use of molecular and conventional techniques to identify and analyze genetic variability of Rhizoctonia spp. isolates

51%
Irzykowska L., Zoltanska E., Bocianowski J.
Acta Agrobotanica
|
2005
|
tom 58
|
nr 2
19-32
In this study the pathogenicity of Rhizoctonia spp. isolates towards wheat seedlings in laboratory and greenhouse conditions was evaluated. In both experiments seven features were examined: plant height, roots weight, the percentage of infected stems and leaf sheaths and also the degree of stem and leaf sheaths infection. Isolates R1, R29, R39 and R59 were the most pathogenic. Percentage of infected stems ranged from 25.3 to 82.5 and roots from 35 to 82.3. The amplification of internal transcribed spacer regions (ITS1 and ITS2) between 18S, 5.8S and 28S rRNA genes and sequence analysis of these regions have been shown to be sufficiently variable to resolve two Rhizoctonia species. Random amplified polymorphic DNA (RAPD) was used to assess genetic variability among isolates. The suitability of RAPD method for isolates differentiation at intraspecific level was shown. Using seven arbitrary primers in polymerase chain reaction (PCR) thirty-three RAPD markers were generated. Clustering analysis from RAPD data resolved two groups of R. cerealis isolates at the 36% similarity level. Moreover, significant associations between molecular markers and pathogenicity of R. cerealis isolates were found.
10

Analiza siedmiu cech ilosciowych pokolenia F4 Pisum sativum L.

51%
Bocianowski J., Irzykowska L., Swiecicki W. K., Wolko B.
Roczniki Akademii Rolniczej w Poznaniu. Rolnictwo
|
2002
|
tom 62
3-14
11

Porownanie wystepowania grzybow na materiale siewnym i na roslinach marchwi pochodzacych z tego materialu

51%
Irzykowska L., Korolewski Z., Weber Z.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 2
109-113
12
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Wykorzystanie zmiennosci markerow izoenzymatycznych do charakterystyki genotypow odmian grochu [Pisum sativum L.]

51%
Irzykowska L., Wolko B., Krajewski P.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
1998
|
tom 463
539-548
Analizę polimorfizmu izoenzymów zastosowano do charakterystyki zmienności genetycznej i identyfikacji odmian hodowlanych grochu. Wybrane linie z banku genów Pisum reprezentowały wszystkie aktualnie zarejestrowane odmiany. Przebadano zmienność 10 systemów enzymatycznych w 21 odmianach grochu biało kwitnącego i 12 barwnie kwitnącego. Polimorfizm wewnątrzodmianowy przynajmniej jednego locus obserwowano w 9 odmianach. Wykryty zakres zmienności izoenzymatycznej 11 loci pozwolił na rozróżnienie wszystkich odmian grochu biało kwitnącego, natomiast identyfikacja wszystkich odmian grochu barwnie kwitnącego wymagała obserwacji polimorfizmu 14 loci. Wyznaczone współczynniki podobieństwa pomiędzy odmianami wykorzystano do dyskusji nad ich pokrewieństwem i zakresem zmienności puli genowej stosowanej w hodowli twórczej. Uzyskane wyniki wzbogaciły charakterystykę genotypów odmian stanowiących część materiałów kolekcyjnych. Tego rodzaju informacje mogą być wykorzystane w pracach hodowlanych do identyfikacji i kontroli czystości odmian grochów podczas produkcji i dystrybucji materiałów nasiennych.
13

Ocena zaleznosci pomiedzy szybkoscia liniowego wzrostu grzybni izolatow Gaeumannomyces graminis var. tritici a ich patogenicznoscia

51%
Irzykowska L., Bocianowski J., Dorozko A.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 2
105-108
14

Interval mapping of QTLs controlling some morphological traits in pea

51%
Irzykowska L., Wolko B., Swiecicki W. K.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 2A
A linkage map of pea was constructed based on a 104 RIL population derived from the cross combination Wt10245 x Wt11238. The map, which consisted of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of the QTLs controlling the stem length and internode number of pea. In the characterization of a given QTL, we included an identification of its position with reference to the flanking markers, an estimation of the part of variance explained by it, and a determination of gene action. Six QTLs per trait were identified as demonstrating linkage to ten intervals on five linkage groups. As many as seven QTLs influencing the analysed traits were mapped on linkage group II, indicating the important role of this region of the pea genome in plant height control.
15
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AFLP i SSR - systemy markerowe przydatne w hodowli roslin

51%
Wolko B., Irzykowska L., Swiecicki W. K.
Postępy Nauk Rolniczych
|
1999
|
tom 46
|
nr 2
59-71
A number of PCR-based methods can be used to detect the polymorphisms in plants. In this article three molecular techniques were compared: RAPD, AFLP and SSR. The marker generating procedures of two systems, AFLP and SSR, were described in details. Advantages and disadvantages each of them and their usefulness in plant breeding projects were discussed. Finally, hitherto applications of AFLP and SSR marker polymorphism analyses for genetical investigations of plants were reviewed.
16
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Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars

51%
Wolko B., Swiecicki W. K., Kruszka K., Irzykowska L.
Journal of Applied Genetics
|
2000
|
tom 41
|
nr 3
17

Occurrence of Fusarium species in spears of asparagus (Asparagus officinalis)

45%
Weber Z., Karolewski Z., Irzykowska L., Knaflewski M., Kosiada T.
Phytopathologia Polonica
|
2007
|
tom 45
18

Grzyby z rodzaju Trichoderma w uprawie pieczarki i boczniaka

45%
Sobieralski K., Siwulski M., Irzykowska L., Sas-Golak I., Grzebielucha I.
Postępy Nauk Rolniczych
|
2007
|
tom 59
|
nr 4
19
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Evaluation of milk thistle (Silybum marianum (L.) Gaertn.) seed germination in relation to seed health and seedling emergence

45%
Rosinska A., Dorna H., Szopinska D., Irzykowska L., Seidler-Lozykowska K.
Herba Polonica
|
2018
|
tom 64
|
nr 3
Milk thistle (Silybum marianum (L.) Gaertn.) is an important medicinal plant. Achenes of milk thistle contain sylimarin, protecting liver cells against toxic compounds. The aim of the research was to find an optimum method of evaluation of milk thistle seed germination. Ten seed samples were tested. The seeds were germinated: on top of blotter paper, on top of blotter paper after seed disinfection, between pleated blotter paper, in rolled blotter paper and in sand. Germination at the first and final counts, the percentages of abnormal seedlings and dead seeds were determined. The correlation coefficients between seed germination, evaluated with various methods, and seedling emergence were calculated. Moreover, fungi associated with seeds and diseased seedlings were identified. The lowest percentage of normal seedlings was observed after germination on the top of blotter. Highly significant positive correlations were noted between seedling emergence and seed germination at the final count evaluated in rolled paper, between pleated paper and in sand. The fungi from genera:Alternaria, Fusarium, Penicillium, Trichoderma, Ulocladium and Verticillium were frequently identified on seeds and seedlings. Infestation with fungi significantly affected milk thistle seed germination and plant emergence. Germination in rolled blotter paper may be recommended for evaluation of milk thistle seed germination, as the most practical and significantly correlated with seedling emergence.
20

Occurrence of Fusarium fungi and mycotoxins in marketable Asparagus spears

39%
Waskiewicz A., Irzykowska L., Bocianowski J., Karolewski Z., Kostecki M., Weber Z., Golinski P.
Polish Journal of Environmental Studies
|
2010
|
tom 19
|
nr 1
219-225
Fusarium oxysporum and/or F. proliferatum were isolated from all asparagus spears with brown spots (which indicate an infection) and from almost all spears without spots. The presence of Fusarium spp. And their toxins in the basal parts of asparagus spears was analyzed. Fumonisin B₁ (FB₁) and moniliformin (MON) were found in spears with brown spots and those without disease symptoms. FB₁ was determined in the concentration range 0.16-152.68 ng g⁻¹ (mean 7.52), while moniliformin was detected in the range 15.30-585.00 ng g⁻¹ (mean 121.00). Only in 10% analyzed spears were metabolites not detected.
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