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The possibilities of using antagonistic fungi and bacteria in the limitation of grapevine stems infection by Phomopsis viticola Sacc. were studied. Trichoderma koningii Oud., T. viride Persoon ex S.F., T. harzianum Rifai, Gliocladium catenulatum Gilman and Abbott, G. fimbriatum Gilman and Abbott, Bacillus sp., Pseudomonas fluorescens and five unidentified isolates of bacteria i.e.: 22a, 35, 40, 45, 66 were estimated. It was appeared what Trichoderma spp. were the most effective in protection of grapevine stems against the infection by P. viticola. After these antagonistic fungi were used on protected grapevine canes not numerous necrosis were observed and few cultures of pathogen were reisolated from them. Moreover, Trichoderma spp. survived on the grapevine stems during the period of experiment. The abilities of other microorganisms tested to protect grapevine cuttings against P. viticola infection and to exist on the stems were less than Trichoderma spp.
Out of 282 isolates of epiphytic bacteria population originating from grapevine leaves 15% inhibited growth of Phomopsis viticola on potato-dextrose agar. Protective activity of 16 isolates, representing different morphological groups was tested on one-year old of grapevine stems fragments. Majority of bacterial isolates which inhibited the fungus growth in-vitro provided no protection of stems fragments against P. viticola. The efficiency of protection activity of bacterial isolates tested decreased with time. The most effective isolates of bacteria in protection of grapevine stems were identified as Bacillus sp. and Pseudomonas fluorescens.
The purpose of the study, carried out in the years 2001–2003 was to determine which fungal species inhabited decaying grapevine cuttings during callusing and soon after planting them into pots. The plant material was collected from 5 commercial plantations and 8 cultivars, which were most frequently cultivated. From each plantation and cultivar 20 cuttings with symptoms of the growth inhibition or decay were randomly sampled during the callusing period i.e. March/April (term I) and 2–3 months after planting the cuttings into pots i.e. June/July (term II). The results showed that from affected grapevine cuttings Phomopsis viticola, Botrytis cinerea, Alternaria alternata and Fusarium spp. were isolated most frequently. Moreover, it was found that after planting young cuttings into the pots, numerous isolates of soil borne pathogenes were obtained, among others Cylindrocarpon spp., Phytophthora sp., Rhizoctonia solani, Fusarium spp.
The purpose of this study, conducted in the years 2000 - 2002, was to identify fungi species colonizing apparently healthy canes and to investigate whether canes storage modify the quantitative and qualitative composition of these fungi. The plant material was collected from 5 commercial plantations growing in various regions of Poland, taking into consideration 8 cultivars which were the most frequently cultivated. From each plantation and cultivar 20 apparently healthy canes were randomly sampled in two terms: before storage - November/December (term I) and 3-4 months after storage - February/March (term II). The results showed that from asymptomatic canes 2746 isolates of fungi belonging to 23 species were obtained, but the majority of them origined from canes analysed after storage. It was found that P. viticola is able to live latently within grapevine tissue in Polish conditions because isolates of this fungus from visually healty canes the all studied plantations and terms were obtained. Among the other fungi species inhabiting grapevine canes Alternaria alternata and Fusarium spp. dominated. Moreover, both in term I and term II Botrytis cinerea, Phoma spp., Epicoccum purpurascens and Cladosporium cladosporioides were frequently isolated, whereas fungi from the genus Acremonium only in the term I. Each time isolates of Trichoderma spp. and Gliocladium spp. were also obtained. Inhabitation of grapevine canes by various fungi species shown in the present experiment indicate the danger of pathogens spread with propagation material on the new plantations.
The effect of nine fungicides and chitosan on the viability of Phomopsis viticola Sacc. spores occurring abundantly in pycnidia on carnation leaves, was tested in the laboratory conditions. The chemicals selected represent different mode of action on the pathogen and they were recognized as very effective in limiting P. viticola development within many viticultural regions. Fungicides were tested at six various concentrations of active ingredients, i.e. 10, 50, 100, 200, 500 mg. a.i./cm3 and at the concentrations that were recommended by the manufacturers. It was found that chlorothalonil, flusilazol and thiophanate – methyl caused the decay of all P. viticola spores in each studied concentration. After using mancozeb, cyprodinil, azoxystrobin and chitosan a strong inhibition of spore viability were also observed, especially at the concentrations of 100 mg of a.i. on cm3 and above. The other fungicides seem to be less effective compared with the above-metioned ones. Basing on the present studies and abundant information from literature, some of the most effective chemicals which should be tested on grapevine plantations in Poland were indicated.
Energy balance and body mass changes were studied in non-reproducing adult eastern hedgehogs Erinaceus concolor (Martin, 1838) during an 8-day feeding trial. The amount of energy consumed by the hedgehogs that maintained constant body mass averaged 0.74 kJ x g_1 x day-1. The energy equivalent of body mass loss amounted to 15.12 kJ x g-1, and that of body mass gain was 25.18 kJ energy consumed x g~ . If the energy contents of body mass gained and lost were the same, biomass production efficiency was 72.2%.
Celem niniejszej pracy było określenie wpływu żywienia dietą wysokotłuszczowej na wskaźniki ogólnowzrostowe i tkankowe poziomy Ca i Mg u szczura. Doświadczenie przeprowadzono na 16 szczurach Wistar, które żywiono przez 8 tygodni dietą kontrolną i wysokotluszczową (25% w/w). Po tym okresie zwierzęta uśpiono i pobrano narządy wewnętrzne do badań biochemicznych. Zawartość Ca i Mg w tkankach oznaczono metodą AAS, po uprzedniej mineralizacji próbek na mokro. Stwierdzono, że żywienie szczurów dietą wysokotłuszczową spowodowało istotnie większy przyrost masy ciała i efektywności żywienia, a zarazem spadek poziomu Mg w wątrobie, ale nie wpływało na tkankowe poziomy Ca.
In recent years, it has been reported the increasing harmfulness of Diaporthe/Phomopsis spp. in the orchards and nurseries worldwide. They cause nonspecific symptoms which in combination with the morphological similarity of the cultures and difficult sporulation creates problems with their correct identification using classical methods. This implies the need to integrate advanced molecular techniques to identify the strain on the species level. The aim of this research was to determine the identity and genetically characterize forty isolates of Diaporthe/Phomopsis obtained in 2010–2012 from the shoots of selected fruit trees grown in the south-eastern Poland. Using the RAPD-PCR technique the genetic similarity within studied population has been demonstrated irrespectively of the origin and type of the host plant from which the isolates derived. Based on the comparative analysis of the non-coding sequence of the ITS regions (ITS1, 5.8S rDNA, ITS2) with the sequences available in NCBI database the studied Diaporthe isolates were identified as Diaporthe eres species complex not described previously on fruit trees under Polish conditions.
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