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1

Role of IL-1beta in production of TNF-alpha and IL-6 by peripheral mononuclear cells following subarachnoid hemorrhage in rats

100%
Larysz-Brysz M., Jedrzejowska-Szypulka H., Olakowska E., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. This leads to numerous pathologies, including increased synthesis of proinflammatory cytokines, like interleukin-1β (IL-1β). Through broken blood-brain barrier, IL-1β may stimulate peripheral leukocytes. These peripheral mononuclear cells (PMC) may be an additional source of other cytokines and migrating to the brain they can enhance or reduce the pathologies resulting from SAH. We examined the effect of neutralization of IL-1β on secretion of TNF-α and IL-6 by PMCs in adult rats following SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies. Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, blood samples were collected from the extraorbital plexus and centrifuged to separate leukocyte subpopulations. Isolated PMCs (monocytes and lymphocytes) were cultured for 24 hrs and TNF-α and IL-6 concentrations in the supernatants were assessed with ELISA. SAH led to the increase of production of both TNF-α and IL-6 by PMCs. Neutralization of IL-1β activity significantly reduced the concentration of both cytokines 90 min as well as 24 hrs after SAH. The results indicate an important role of IL-1β in the activation of peripheral mononuclear cells in the course of subarachnoid hemorrhage.
2

Peripheral nerve extracts change their neuroprotective activity upon CNS neurites after NGF blockade

100%
Larysz-Brysz M., Lewin-Kowalik J., Gorka D., Wolwender K.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
3

Role of bcl-2 in the optic nerve regeneration induced by peripheral nerve grawts

100%
Wolwender K., Lewin-Kowalik J., Larysz-Brysz M., Fus Z.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
4

Prevention of epidural fibrosis by a chitosan and/or alginate gel in a rat laminectomy model

88%
Marcol W., Wlaszczuk P., Palen P., Larysz-Brysz M., Leiwin-Kowalik j.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Epidural fibrosis is a frequent complication of lumbar disc surgery, however its influence on incidence of failed back surgeries remains controversial. A bilateral laminectomy (L2–L4) with associated disc injury was performed in 32 adult male Wistar rats. In three experimental groups (n=8 each), spinal cord on laminectomy site was covered with thin layer of gel: alginate, chitosan, or mixture of both. Control group (n=8) was left without treatment. After 4 or 8 weeks, rats (n=4 for each group) were sacrificed and spinal lumbar segments with surrounding muscles were removed and prepared for histologic analysis. Transverse and longitudinal sections were subjected to Masson-Goldner Trichrome staining and examined for severity of epidural fibrosis. Epidural scarring of variable density was found in all laminectomy sites. All experimental groups showed less epidural fibrosis, dural adhesion, fibroblast density, foreign body reaction, and nerve root retraction as compared to the control group. However, no significant differences were found between experimental groups. We suggest that both chitosan and alginate gels form effective barriers for collagen penetration preventing spinal cord from epidural fibrosis following disc injury.
5

IL-1betta stimulates cytokine secretion by peripheral leukocytes following subarachnoid hemorrhage in rats.

88%
Jedrzejowska-Szypulka H., Woszczycka-Korczynska L., Olakowska E., Larysz-Brysz M., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Much evidence indicates that pathologies observed after subarachnoid hemorrhage (SAH), including vasospasm and neurological defects, are the effects of immuno-infl ammatory processes accompanying the hemorrhage. Following SAH, CSF concentration of cytokines increase, what may change the cerebral perfusion and destroy brain–blood barrier. These cytokines are produced by activated leukocytes as well as hypoxic cells in the brain. The aim of this study was to examine the role of IL-1β in stimulation of peripheral leukocytes to increase secretion of cytokines after SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies (SAH’ groups). Control group consisted of sham-operated rats. Ninety minutes or 24 h following surgery, blood samples were collected and subpopulations of mononuclear leukocytes were isolated and cultured for 24 h. Then, the concentration of TNF-α, ET-1 and IL-6 in supernatants was measured by means of ELISA. SAH resulted in signifi cant increase in TNF-α and ET-1, especially after 24 h, and no change in IL-6 concentration. Inhibition of IL1-β decreased the secretion of TNF-α and ET-1 and led to increase in IL-6. We found that peripheral leukocytes are an additional source of cytokines following SAH, and their activation depends on IL1-β produced after hemorrhage.
6

Positive effects of microcrystalic chitosan application after laminectomy in rats

88%
Larysz-Brysz M., Marcol W., Slusarczyk W., Malinowska-Kolodziej I., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
7

Content and composition of proteins obtained from short-time-predegenerated distal stumps of rat sciatic nerves

88%
Gorka D., Larysz-Brysz M., Lewin-Kowalik J., Krause M., Barski J. J.
Applied Biology Communications. Lublin Scientific Center
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1994
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tom 04
|
nr 3-4
8

The effects of peripheral nerve estracts versus BDNF on retinal ganglion cells survival following injury

76%
Golka B., Swiech-Sabuda E., Larysz-Brysz M., Gorka D., Golka D., Marcol W., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
9

Does restruction of jugular venous outflow induce multiple sclerosis-like signs in rat brains?p.S66-S67

76%
Jedrzejowska-Szypulka H., Kotulska K., Kapustka B., Laczynski M., Larysz-Brysz M., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Hypothesis that multiple sclerosis (MS) may be caused by chronic cerebrospinal venous insufficiency (CCVI) has gained public interest from both patients and physicians. However, there’s still lack of evidence for it. We have investigated presence of neuronal demyelination and degeneration, similar to these found in MS, in rat model of CCVI created by occlusion of jugular veins (JVs). METHODS: Twenty-five young female Wistar C rats were used. Complete ligation of both JVs (BO group), left JV (UO), or partial ligation (stenosis resulting in ~70% reduction of blood flow) of both JVs were performed. Blood flow in JVs was measured with Laser Doppler Flow Assessment. Neurological assessment using Neurologic Deficit Scale (NDS), 5-point EAE staging protocol, and gait analysis with CatWalk was performed. After 12 weeks, MRI for detecting demyelinating plaques as well as signs of bloodbrain barrier (BBB) disruption was performed. Histologic analysis of brain specimens was focused on markers of inflammation and demyelination. RESULTS: No neurologic deficits were found in all experimental animals. Both NDS, EAE and gait analysis did not differ from normal. MRI T2- and T1- weighted imaging as well as FLAIR sequence did not reveal any abnormalities in the brains of experimental rats. Histological analysis did not show any signs of inflammation or demyelination. CONCLUSIONS: Twelve-week CCVI in rats, both complete and partial, did not induce any changes resembling pathologies observed in MS. Therefore, linking CCVI with origin of MS remains controversial.
10

The new tool for precise transection of peripheral nerves and soft tissues-blade with graded bioactive surface

76%
Lewin-Kowalik J., Marcol W., Miodonski J., Rajchel B., Pyza E., Wlaszczuk A., Larysz-Brysz M.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Current methods of peripheral nerve cutting are highly unsatisfactory. Imperfect cutting plane directly influences the effect of nerve anastomosis. For precise rejoining of cut nerve stumps directly, or to bridge large gap with autologous nerve graft, the surfaces of nerve stumps must be even and perfectly matching. Actual methods still do not provide such undisturbed pathway for regenerating fibers. In this study, we examined the in situ morphological properties of peripheral nerves cut with an innovative device. METHODS: Rat and rabbit sciatic nerves were cut with the carbonmetal coated blade with bioactive surface mounted on the highspeed dental drill. Transected nerves were subjected to immediate analysis of the plane surface. It was performed using the scanning electron microscope, and standard H-E as well as Masson-trichrome stainings of the serial transverse sections. Analysis was performed by two experienced certified histopathologists. RESULTS: The procedure provided a section area devoid of unevenness or shreds of epineurium or perineurium. Moreover, individual axons seemed to be cut smoothly. The scanning electron microscope images showed the transected axons arranged paralelly to the main axis as well as other undisturbed components of the nerve (i.e. blood vessels and Schwann cells). Also, there was no thermal damage of the cutting plane. CONCLUSION: This technique holds a promise for the development of a minimally invasive alternative approach that utilizes already available technology and equipment, with cutting plane allowing for perfect matching of nerve stumps subjected for rejoining.
11

Promotion of the optic nerve regeneration and survival of retinal ganglion cells by the extracts from peripheral nerves when compared to BDNF

76%
Swiech-Sabuda E., Galka B., Lewin-Kowalik J., Larysz-Brysz M., Gorka D., Marcol W.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
12

Purified extracts from predegenerated peripheral nerves enhance the regrowth of optic nerve fibres and survival of retinal ganglion cells

76%
Swiech-Sabuda E., Golka B., Lewin-Kowalik J., Larysz-Brysz M., Gorka D., Krause M.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
13

Cu/Zn-SOD overexpression impairs regeneration and aggravates neuropathic pain after sciatic nerve injury

76%
Lewin-Kowalik J., Kotulska K., London J., LePecheur M., Paly E., Golka B., Larysz-Brysz M.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
14

Activated schwann cells delivered into the subarachnoid space via cisterna magna induce repair of injured rat spinal cord white matter

76%
Lewin-Kowalik J., Marcol W., Jedrzejowska-Szypulka H., Slusarczyk W., Smetek J., Sordyl R., Larysz-Brysz M.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
We investigated the influence of activated Schwann cells on regeneration of the spinal cord in rats. Young adult male Wistar C rats were used (n=36). Focal injury of spinal cord white matter at Th10 level was produced using our original non-laminectomy method by means of high-pressured air stream. Schwann cells obtained from 7-days-predegenerated rats’ sciatic nerves (n=3) were cultured, transfected with GFP and injected into cisterna magna (S group, n=23) three times: immediately after spinal cord injury and 3 as well as 7 days later (300 000 cells/injection). Control animals (n=10) were subjected to the spinal cord injury only. Neurons in brain stem and motor cortex were labeled with FluoroGold (FG) delivered caudally from the injury site and transported via spinal ascending tracts a week before the end of experiment. Functional outcome (BBB scale, Sciatic Functional Index) and morphological features of regeneration were analyzed during 12-week follow-up. The lesions were characterized by means of MRI. Maximal distance of expansion of implanted cells in the spinal cord was measured and the number of FG-positive neurons was counted. Rats treated with Schwann cells presented significant improvement of locomotor performance when compared to the control group. MR images showed no cyst in the spinal cord in S group, while in the control group changes resembling typical post-traumatic syringomyelia were found. The sizes of lesions were also significantly smaller in S group. Distance covered by Schwann cells was 12 mm from the epicenter of injury. Number of brain stem and motor cortex FG-positive neurons in S group was significantly higher than in control group. Obtained data revealed that activated Schwann cells are able to induce the repair of injured spinal cord white matter. The route of cells application via cisterna magna appeared useful for their delivery into the injury area.
15

Matrix metalloproteinases activity in distal stumps of rat sciatic nerves 1-7 days following transection

76%
Pietrucha-Dutczak M., Lewin-Kowalik J., Gorka D., Korczynska I., Matuszek I., Wolwender K., Larysz-Brysz M.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
16

Functional impairment of nerve regeneration in trkBplus/minus mice

76%
Lewin-Kowalik J., Kotulska K., Marcol W., Larysz-Brysz M., Malinowska-Kolodziej L., Golka B.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
17

Peripheral nerve grafts implantation into the optic nerve and their influence on the regrowth of fibres and survival of retinal ganglion cells

76%
Golka B., Lewin-Kowalik J., Larysz-Brysz M., Gorka D., Swiech-Sabuda E., Krause M.
Acta Neurobiologiae Experimentalis
|
1997
|
tom 57
|
nr 5
18

Postmicrosomal protein fractions from short-time-predegenerated rat sciatic nerve

76%
Gorka D., Lewin-Kowalik J., Larysz-Brysz M., Golka B., Swiech-Sabuda E., Malecka-Tendera E.
Acta Neurobiologiae Experimentalis
|
2000
|
tom 60
|
nr 4
The postmicrosomal protein fraction obtained from distal stumps of rat sciatic nerves at 0-6 days following transection were investigated by means of one- and two-dimensional electrophoresis. In all experimental groups, total amount of protein was significantly higher than in the control group. Proteins were resolved into 27 bands after SDS-PAGE. Their molecular weights ranged between 16.2 and 335.4 kDa. Eleven fractions displayed significant quantitative differences. After 2-D-electrophoresis, the pI of the proteins ranged from 4.2 to 7.4. They were resolved to 28 molecular masses from 13.5 kDa to 335.4 kDa. The greatest numbers of fractions (90-109) were observed on the 3rd, 4th, 5th and 6th day after nerve transection. Thus, during first 6 days after transection intensive changes in protein fraction content and composition take place in the distal stump of peripheral nerve. These processes seem to be most prominent on the 4th day after lesion. Results confirm our earlier in vivo findings.
19

Regeneration of sciatic nerves of adults rats induced by extracts from distal stumps of peripheral nerves

64%
Marcol W., Lewin-Kowalik J., Larysz-Brysz M., Swiech-Sabuda E., Gorka D., Golka B., Wolwender K., Pietrucha-Dutczak M.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
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