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1

Characterization of olive seed storage proteins

100%

Wang W., de Dios Alche J., Rodriguez-Garcia M. I.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 5

At present little is known about olive seed storage proteins (SSPs). A better understanding of olive SSPs will be important for future biotechnology efforts. In the present study, we first developed a protocol relied on chloroform for preparing protein samples free of lipids from lipid-rich olive seeds. Then, we characterized olive SSPs by SDS-PAGE, N-terminal sequencing and immunoblot. Two smaller subunits (20 and 21.5 kD) of SSPs were purified to homogeneity and used for antibody production or N-terminal sequencing. N-terminal sequencing confirmed that major olive SSPs are 11S globulins. Moreover, the components and size distribution of SSPs are identical among several olive cultivars examined, suggesting that their synthesis is highly conserved in this species. Olive SSPs are soluble in aqueous alcohol, with limited solubility in water and dilute salt. Thus, despite their homology with globulins, olive SSPs are similar in solubility to prolamins and different from globulins in other dicot plants. Finally, the accumulation of olive SSPs during fruit maturation was examined. Our results revealed that the accumulation of SSPs is time-dependent and tissue-specific, and only 105 days after pollination (DAP), did individual components of SSPs synthesize substantially, and accumulate rapidly in large quantities over a short period of time. Our results suggest that a 36 kD protein is the precursor of olive SSPs, and 90–105 DAP seems to be a crucial transition period (from a precursor to mature subunits) for the accumulation of SSPs.

2

Profilin variability in olive (Olea europaea L.) pollen cultivars

100%

Jimenez-Lopez J. C., Morales S., de Dios Alche J., Rodriguez-Garcia M. I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

3

Ole e 10, a 1,3-β-glucanase from olive pollen, co-localizes to callose during pollen tube growth

88%

Suarez C. G., Barral P., de Dios Alche J., Rodriguez R., Rodriguez-Garcia M. I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

4

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Ethylene-dependent effects on generative organ abscission of Lupinus luteus

76%

Frankowski K., Kucko A., Zienkiewicz A., Zienkiewicz K., de Dios Alche J., Kopcewicz J., Wilmowicz E.

Acta Societatis Botanicorum Poloniae

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2017

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tom 86

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nr 1

The abscission of certain organs from the plant is part of the fulfilment of its developmental programs. The separation process occurs in a specialized abscission zone usually formed at the base of detached organ. The changing level of phytohormones, particularly ethylene, is the element responsible for coordinating anatomical and physiological transformation that accompanies organ abscission. The application of ethylene (ET) on Lupinus luteus stimulates flower abortion. However, the treatment with 1-aminocyclopropane-1-carboxylic acid (ACC) – direct ET precursor – does not cause such a strong physiological response. In turn, when applied on the pedicels both ET biosynthesis (2-aminoethoxyvinylglycine; AVG) and action (norbornadiene; NBD) inhibitors reversed the stimulatory effect of ET on generative organ separation. In order to determine ET role in the flower abscission process in L. luteus, we identified the sequences coding for synthase (LlACS) and oxidase (LlACO) of ACC and measured their expression levels. Abscission zone activation is accompanied by a considerable increase both in LlACS and LlACO cDNAs and also ACC content, which is specifically localized in the dividing cells at the base of the flower being detached. Obtained results suggest that ET is a strong stimulator of flower abortion in L. luteus.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Acta Physiologiae Plantarum

1 Acta Societatis Botanicorum Poloniae

4 de Dios Alche J.

3 Rodriguez-Garcia M. I.

1 Barral P.

1 Frankowski K.

1 Jimenez-Lopez J. C.

1 Kopcewicz J.

1 Kucko A.

1 Morales S.

1 Rodriguez R.

1 Suarez C. G.

1 Wang W.

1 Wilmowicz E.

1 Zienkiewicz A.

1 Zienkiewicz K.

1 2017

1 2007

2 2005

Characterization of olive seed storage proteins

Profilin variability in olive (Olea europaea L.) pollen cultivars

Ole e 10, a 1,3-β-glucanase from olive pollen, co-localizes to callose during pollen tube growth

Ethylene-dependent effects on generative organ abscission of Lupinus luteus