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Phoma strasseri is one of the major pathogen of peppermint (Mentha piperita). An important criterion in the classification of Phoma spp. is conidiogenesis, which has never been studied in P. strasseri before. The analysis using a FEI Tecnai Spirit G2 electron microscope revealed enteroblastic phialidic ontogeny as a type of conidiogenesis present in this species. Conidia are formed on small conidiogenous cells, called phialides. The tip of the conidiogenous cell is surrounded by a characteristic collarette. The surface of the conidium wall is covered with a mucilaginous sheath, which may enable conidia to attach to the plant surface.
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New Phoma species on Leonurus cardiaca

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Two species of Phoma obtained from motherwort Leonurus cardiacaL., during mycological analyses attendant upon three-years study connected healthiness of the plants. Isolates of Phoma capitulum were obtained from roots, whereas Phoma septicidalis from roots and leaves. Description in vitro, photos of morphological structures and distribution of Phoma spp. are given. This is the first report of P. capitulum and P. septicidalis on motherwort in Poland.
Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had infected the stems and rhizomes of peppermint. Of all inoculation methods, direct placement of colonized agar plugs on damaged epidermis and soaking stems and rhizomes in conidial suspension were the most e#ective. !e behavior of the conidia deposited on the stems and rhizomes was investigated at different time intervals after inoculation: 6, 16, 24, 36 and 48 h. Conidia produced an appressorium directly at the end of a short germ tube. Appressoria were formed over the cuticle, but never over stomata. Direct penetration to host tissue through the cuticle was observed. The spore and hyphae were covered with a mucilaginous sheath.
Fungi belonging to Phoma spp. commonly occur on herbaceous plants, including herbal ones. In the years 2004–2006 isolates belonging to genus Phoma which were differentiated in morphological respects were obtained from herbs belonging to family Lamiaceae. Basing on constant morphological feature observed in vitro in the cultures developing on standard media, isolates of Phoma spp. were included within the proper section, and next the species were identified. P. exiqua var. exiqua was isolated from all parts of the examined plants. P. nepeticola cultures were obtained from the leaves and stems of motherwort, whereas isolates of P. nemophilae were from the roots. Apart from the above mentioned species, P. capitulum, P. labilis, P. pereupyrena, P. subglomerata and P. septicidalis were also isolated.
Phoma strasseri was isolated first time from peppermint (Mentha piperita L.) in 2004. These species had not been found in Poland earlier. Biotic interactions between P. strasseri and 16 species of fungi colonizing the phyllosphere of stems and rhizomes of peppermint were determined using the biotic series method and maltose agar MA. The effect of particular fungi species on P. strasseri was expressed as and individual, general and summary biotic effect. Fungi from genera Trichoderma were found out to be the most effective and positive antagonists whereas those of Alternaria alternata, Botrytis cinerea and Rhizoctonia solani – despite the high values of IBE – were considered negative antagonists. Studies on the effect of thermal conditions pointed out that the thermal optimum for the growth of the fungus colonies ranged from 16℃ to 28℃, while that for the formation of the infectious material from 24℃ to 28℃. Basing on the ability of P. strasseri to develop in a wide range of temperatures, it was included within the group of eurythermic organisms.
Pathogenicity and ultrastructural investigation of the ribwort leaves inoculated with Phyllosticta plantaginis conidia was undertaken using scanning electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. plantaginis isolates had infected leaves of ribwort. Of all inoculation methods, direct placement of colonized agar plugs on damaged epidermis and soaking leaves in conidial suspension were the most effective. The behavior of the conidia deposited on the leaves was investigated at different time intervals after inoculation: 7, 18, 25, 48 and 72 h. An appressorium appeared directly at the end of a short germ tube grown from conidia. Appressoria were formed over the cuticle in some distance from the stomata. Penetration through the stomata was observed.
Acta Agrobotanica
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2002
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tom 55
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nr 1
401-410
The present study deals with effects of the air temperature, and the type of medium on the growth and form morfological structures of six Seimatosporium hypericinum isolates tested. St Jonh's Wort extract agar, St Jonh's Wort plant agar and PDA, oatmeal agar has been recognized as most useful for growth and sporulation of S. hypericinum. Mineral agar, appeared the least useful for growth and form acervuli and conidia. S. hypericinum can develop in a wide range of temperature, but the optimum one for the growth and sporulation of the fungus vary between 20°C and 28°C.
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Studies on the diversity of fungi colonizing and damaging selected plant parts were conducted in 2007–2009. They were performed on productive plantations of savory (Satureja hortensis L.) grouped in south-eastern Poland. Fungi were isolated from superficially disinfected roots, stems and leaves by means of a mineral medium. Fungi from Fusarium spp., Phoma spp. and Rhizoctonia solani were obtained from the roots and stem bases showing necrosis and tissue narrowing. Botrytis cinerea was obtained from the stems with the symptoms of necrosis, breaking and tissue disintegration.
Studies on diversity of fungi colonizing and damaging selected plant parts of ribwort (Plantago lanceolata L.) were carried out in 2009–2011 on plantations grouped in south-eastern Poland. Fungi were isolated from superficially disinfected roots, collar roots and leaves by means of a mineral medium. Fungi from Fusarium spp., Boeremia exigua var. exigua comb. nov. and Rhizoctonia solani were obtained from the roots and collar roots showing necrosis and tissue decay. Phyllosticta plantaginis was commonly obtained from the leaves with symptoms of small, regular, necrotic spots. This species had not been found in Poland earlier.
Studies on diversity of fungi colonizing and damaging different plant parts of oregano (Origanum vulgare L.) were carried out in 2012–2014 on production plantations grouped in south-eastern Poland. Fungi were isolated from superficially disinfected roots, stems and leaves using mineral medium. Fungi from Fusarium spp., Boeremia exigua var. exigua and Rhizoctonia solani were obtained from the roots and stems showing necrosis and tissue disintegration. Alternaria alternata and Stemphylium botryosum were isolated from the leaves with symptoms of irregular, necrotic spots. Colletotrichum fuscum was commonly obtained from the leaves showing symptoms necrotic, concentrically zoned spots with lighter center and the slightly raised edge. This species had not been found in Poland earlier.
In 2004–2006 there were studies conducted on fungi colonizing and harming different parts of plants on plantations of sage (Salvia officinalis L.) grouped in south-eastern Poland. Fungi were isolated from roots, the stem base and leaves. The surface was disinfected by means of a mineral medium. Fungi from genera Fusarium, Rhizoctonia solani, Phoma exigua var. exigua were isolated from roots and the lower parts of stems with the symptoms of necrosis and tissue disintegration. The species Phomopsis sclarea, which had not been observed earlier in Poland, was obtained from the stems with the symptoms of necrosis, peeling off and bark breaking. Altenaria alternata was commonly isolated from the leaves with necrotic symptoms.
Biological activity of chitosan in the form of a microcrystalline gel and of a hydrogel, as well as the activity of grapefruit extract were evaluated in vitro by means of measuring the growth inhibition and formation of morphological structures of four isolates of Seimatospo- rium hypericinum, obtained from leaves and stems of St. John's wort (Hypericum perforatum). In addition, the effect of acybenzolar S-methyl was tested in climatic chamber conditions, when plants inoculated by S. hypericinum were protected. The studies carried out in vitro showed that both chitosan and grapefruit extract inhibited growth of pathogen colonies significantly. The studies confirmed the protective effect of chitosan, grapefruit extract and acybenzolar S-methyl in the case of St. John's wort plants inoculated by S. hypericinum. Chitosan and grapefruit extract proved to be the most effective when used preventively.
The object of the research work was an isolate of S. hypericinum D 1224, as well as ten fungicides belonging to different chemical groups. The studies on the toxic effect were conducted in vitro by means of poisoning the media with the use of 1, 10 and 100 μg a.i./cm3 of the tested preparations. The obtained results showed that the compounds of thiophanatemethyl and triadimephon caused complete inhibition of the growth of S. hypericinum, when the dose of 10 and 100 μg a.i./cm3 was used, while in the case of mancozeb the effective dose was 100μg a.i./cm3. These compounds, as well as trifloxystrobin, can be therefore regarded as a prospective means of limiting growth and development of S. hypericinum and protecting St. John’s wort from this pathogen. The effect of the other compounds on S. hypericinum was less toxic.
Studies on biodiversity of fungi colonizing and damaging selected plant parts were conducted in 2004–2006. They were performed on productive plantations of motherwort (Leonurus cardiaca L.) grouped in south-eastern Poland. Fungi were isolated from superficially disinfected roots, stem bases and leaves by means of a mineral medium. Fungi from Fusarium spp., Phoma spp. and Rhizoctonia solani were obtained from the roots showing necrosis and tissue disintegration. Phoma nepeticola was commonly obtained from the leaves and stems with symptoms of small, regular, necrotic spots. This is reported for the first time in Poland.
The paper is concerned with the pathogenicity of four isolates of Seimatosporium hypericinum towards the leaves, stems and seeds of St. John's wort (Hypericum perforatum L.). The conidial spores of the tested fungus isolates growing on a glucose-potato nutrient medium were used in inoculation of the seeds stratified at the temperature of 0°C and disinfected superficially. The infection suspension contained 10" conidia/1 ml of sterile distilled water. The leaves and stems were infected through the injured and non-injured epidermis. The infection material consisted of 3 mm rings of the mycelium from 14-day-old sporulating cultures of the examined isolates of S. hypericinum. Artificially infected and control seeds and leaves were kept in moisture chambers at temperatures of 27°C and 22°C, respectively. Inoculation of the stems of St. John's wort took place in a climatic chamber. Positive results of reisolation of S. hypericinum from the artificially infected leaves and stems showed a possibility of infecting those parts by the tested isolates of the fungus. On the other hand, it was not possible to reisolate the fungus cultures from the artificially infected seeds of St. John's wort.
In 2004–2006 there were studies on fungi colonizing and damaging different plant parts conducted. They were performed on productive plantations of peppermint (Mentha piperita L.) grouped in South-Eastern Poland. Fungi were isolated from superficially disinfected roots, rhizomes, stem bases and leaves by means of a mineral medium. Fungi from Fusarium spp. including F. avenaceum, F. equiseti, F. culmorum, F. oxysporum were obtained in the greatest number from roots showing necrotic symptoms. The Phoma strasseri was commonly isolated from rhizomes and stem bases with symptoms of necrosis and tissue disintegration. Altenaria alternata was isolated from leaves showing symptoms of necrotic, irregular spots.
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