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Effects of Se deficiency on serum histamine concentration and the expression of histamine H2 receptor in the jejunum of chickens

100%

Wang G., Wang H., Wang H.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

The present study was designed to investigate the influence of Se deficiency on serum histamine concentration and the expression of histamine receptor in the jejunum of chickens. Forty neonatal chickens were randomly divided into two groups. Experimental chickens were fed a low-Se diet (0.034 mg/kg), whereas chickens in the control group were fed a diet with a Se level of 0.229 mg/kg. Ten chickens were sacrificed on days 30, 45, 60 and 75. Blood and jejunum samples were collected. Histamine concentration in the jejunum was measured by ELISA, the jejunal mast cell (MC) ultrastructure was studied by transmission electron microscopy, and the expression level of histamine H2 receptor (H2R) mRNA in the jejunum was examined using real-time PCR. Results: The jejunal histamine concentration in chickens fed the low-Se diet was significantly higher than that in the control group (P<0.01). Se deficiency induced degranulation of MC in the jejunum of chickens in the low-Se diet group; their cytoplasm was filled with fused granules and vacuoles. The expression level of jejunal H2R mRNA in chickens fed the low-Se diet was also significantly higher than that in the control group (P<0.01). The results obtained suggest that Se deficiency stimulates MC degranulation and release of histamine, binding H2R promotes both regulation of digestion and cell proliferation while protects the jejunum from injury induced by Se deficiency.

2

Sensitivity analysis of the effects of climate change on streamflow using climate elasticity in the luan river basin, China

89%

Wang H., He K.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 2

Streamflow depends directly on climate conditions (e.g., precipitation and potential evapotranspiration, or Ep), which affect water balance at the basin scale. The present study was conducted to investigate the impact of climate change on streamflow in the Luan River basin, China. To assess the impact of climatic variation on streamflow, the temporal trends of streamflow were explored using the Mann-Kendall method and the sensitivities of streamflow to precipitation, potential evapotranspiration, and the aridity index were evaluated with the modified method of climate elasticity. The results showed that the average coefficients of sensitivity of streamflow to precipitation and potential evapotranspiration were 3.23 and -2.23, respectively, indicating that a 10% increase in precipitation or potential evapotranspiration would lead to a 32.3% increase or a 22.3% decrease in streamflow, respectively. Additionally, the average coefficient of sensitivity of streamflow to the aridity index was -2.53, which indicated that streamflow would decrease by 25.3% with a 10% increase in the aridity index. The average coefficients of the sensitivity of streamflow to climatic variation appeared to have an inverse relationship with the runoff coefficient, showing that the lower the streamflow of the basin became, the more sensitive the streamflow would be with respect to climatic variation.

3

Ferret badger Melogale moschata activity, movements, and den site use in Southeastern China

89%

Wang H., Fuller T. K.

Acta Theriologica

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2003

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tom 48

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nr 1

We conducted field studies of ferret badgers Melogale moschata (Gray, 1831) during March-November 1994 and May-July 1996 at Taohong Village in southeastern China. Capture data suggested that they gave birth in May, and telemetry data on 11 individuals indicated that they were nocturnal. Size of resting home ranges (daybed locations only; n> 20 re-locations) of 6 individuals averaged 11 ha (range 1-25 ha) and no sex-specific differences in size were detected. Distances between daily resting sites averaged 101 m, but ferret badgers often (51% of occasions) returned to sites used the previous day. Ferret badgers readily used a variety of shelters as daybeds, including rodent dens (47%), firewood stacks (20%), open fields (17%), and rock piles (5%) around houses.

4

Determition of gentiopicroside contents during somatic embryogenesis in Gentiana straminea Maxim

72%

Yang L., Wang H., He T.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 07

A protocol for regeneration of Gentiana straminea Maxim recently established in our laboratory, somatic embryogenesis was obtained from its leaf derived calli. The gentiopicroside contents of embryogenic calli, globular-, heart-, torpedo-, and cotyledon-shaped embryoids as well as regenerated plantlets were determined by high-performance liquid chromatography. Gentiopicroside was detectable in all materials tested. Embryogenic calli showed the lowest gentiopicroside content. The changes of gentiopicroside contents were not significant (P<0.05) with the development of somatic embryos. The highest gentiopicroside content (30.7 mg g⁻¹ dry weight) was achieved in regenerated plantlets. The contents of gentiopicroside were not significant (P<0.05) differences between control plants and embryogenic calli, different stages of somatic embryos and regenerated plantlets. This protocol could be employed for producing gentiopicroside or other medicinal compounds.

5

Addendum to “Stokes transport in layers in the water column based on long-term wind statistics: assessment using long-term wave statistics”

72%

Myrhaug D., Wang H., Holmedal L. E.

Oceanologia

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2019

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tom 61

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nr 4

6

Stokes transport in layers in the water column based on long-term wind statistics

72%

Myrhaug D., Wang H., Holmedal L. R.

Oceanologia

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2018

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tom 60

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nr 3

7

Genome-wide identification of pear HD-Zip gene family and expression patterns under stress induced by drought, salinity, and pathogen

72%

Wang H., Lin J., Li X. G., Chang Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 09

Homeodomain-leucine zipper (HD-Zip) transcription factor genes are unique in the plant kingdom. Studies have shown that HD-Zip transcription factor has four subfamilies (subfamilies I–IV), and each subfamily exerts similar or diverse functions in the growth and development as well as environmental stress responses of plants. Although a genome-wide analysis of this transcription factor has been performed in some species, systematic identification of sequences and expression patterns under biotic and abiotic stress have not been carried out in pear. In this study, we identified 52 putative HD-Zip genes within the pear genome. Based on the phylogenetic analysis, HD-Zip transcription factors were classified into four subfamilies: 18, 13, 5, and 16 members were identified separately in subfamilies I, II, III, and IV, respectively. Genes in the same subfamily shared the constituent structure and conserved motifs, thereby implying that the HDZip genes that shared similar structures may exert similar functions. Moreover, abundant stress-related and pathogenrelated cis-elements were observed in the promoter region of the pear HD-Zip I and II genes. Transcript abundance level of 20 selected HD-Zip I and II genes were analyzed by quantitative real-time RT-PCR (qRT-PCR) during the infection of Alternaria alternata, and abiotic stress conditions such as drought and salinity treatment. The results confirmed that some genes display stress-inducible and pathogen-inducible expression patterns.

8

Effects of xylazine on thyroid hormones, insulin, and glucagon in dogs

72%

Gao L., Zhang Z., Zhang J., Wang H.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 3

In this study, the effects of xylazine on serum levels of triiodothyronine (T3), tetraiodothyronine (T4), insulin (INS), and glucagon (GN) in dogs were investigated. The dogs before injection were used as control group (0 h). The dogs were injected with xylazine at 3 mg/kg, then blood was collected from the peripheral veins at 0.5, 2, 8, 24, 48, 72, and 120 h after the injection. Serum T3, T4, INS, and GN were measured by ELISA. The results revealed that the T3 level decreased in serum 0.5 h after the injection (P<0.05), while the change in T4 was not significant. The secretion of INS increased 8 h after the injection (P<0.05). The GN level increased 2 h and 8 h after the injection (P<0.05). However, all of these changes returned to the norm after 24 h.

9

Collaborative innovation of low-carbon technology from the triple helix perspective: exploring critical success factors based on DEMATEL-ISM

72%

Cui H., Zhu X., Wang H.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 2

10

Sea spray aerosol flux estimation based on long-term variation of wave statistics

72%

Myrhaug D., Wang H., Holmedal L. E.

Oceanologia

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2015

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tom 57

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nr 3

11

Ecological strategy at cell size level to respond to stressed environments

72%

Wang H., Liu Y., Chen H.

Polish Journal of Ecology

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2016

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tom 64

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nr 2

The cell size variations in woody plants in various environments are not well known, and the underlying mechanism has not been fully analyzed. 2. The current study focused on the intraspecific and interspecific size variations in palisade cells occurring in 76 woody species along an elevation gradient (1800–4500 m a.s.l.) on Gongga Montain (1400–7552 m a.s.l.) in southwest China, which included tropical and subtropical genera of broad-leaved forest flora including Salix hyperba as the species occurring on all elevations. We hypothesized that cell size is regulated by alterations in width-length scaling (L-D ratio) to mediate a surface-to-volume ratio (S/V) most suitable for the prevailing environmental conditions. 3. It was observed that cell size co-varied similarly with environmental conditions at the conspecifics, congenerics, and interspecifics levels investigated. Cell sizes decreased among conspecifics, or increase among congenerics and interspecifics via negative or positive scaling of width-length ratio at the cellular level. However, this dichotomously differing tendency may be ecologically constrained by both a cost-increasing and benefit-diminishing mechanism of cell dimension with increasing elevation at the species level, implying a ‘middle way’ strategy for species to stressed environments. 4. The implications of the results for regional floristic evolution are discussed.

12

Effects of embedded gravel or gravel mulching in Southern red soil on slope sediment yield and runoff

72%

Wang H., Lu D., Wang Q., Shan C.

Polish Journal of Environmental Studies

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2021

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tom 30

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nr 1

13

A novel alkaline protease from wild edible mushroom Termitomyces albuminosus

72%

Zheng S., Wang H., Zhang G.

Acta Biochimica Polonica

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2011

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tom 58

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nr 2

A protease with a molecular mass of 30 kDa and the N-terminal sequence of GLQTNAPWGLARSS, was isolated from fresh fruiting bodies of the wild edible mushroom Termitomyces albuminosus. The purification protocol included ion exchange chromatography on DEAE-cellulose, Q-Sepharose, SP-Sepharose and FPLC-gel filtration on Superdex 75. The protein was unadsorbed on DEAE-cellulose and Q-Sepharose, but adsorbed on SP-Sepharose. The optimal pH and temperature of the purified enzyme were 10.6 and 60°C, respectively. The enzyme was stable in the presence of 2% (v/v) Tween 80 and 4 M urea. More than 80% of the enzyme activity was retained in 2% (v/v) Triton X 100, 54% in 10 mM EDTA and 31% in 2% (w/v) SDS. The enzyme was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF), but not inhibited by dithiothreitol (DTT), pepstatin or lima bean trypsin inhibitor suggesting that it was a serine protease but not a trypsin-like one. The protease was inhibited by Hg2+, Cu2+, and Fe3+ ions. The Km and Vmax values of the purified enzyme for casein were 8.26 mg∙ml−1 and 0.668 mg∙ml−1∙min−1, respectively.

14

Presence of Shiga toxin-producing Escherichia coli 0157:H7 and the Stx2 gene and antibody response to Shiga toxin 2 in dairy cattle in Chongqing, People Republic of China

72%

Ding H., Wang H., Mao X., Zou Q.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

205-208

This study intended to acquire the data on Escherichia coli O157:H7 isolated from dairy cattle, heifers, and calves, and of Stx2 expressed in STEC in Chongqing. Three hundred cattle rectal faecal samples were collected and E. coli O157:H7 was identified by the isolation of the bacteria, serological tests, and PCR. Moreover, the stx2 gene's genome was examined. Three hundred and twenty six milk samples were tested for the presence of immunoglobulin G antibodies to Stx2. Six E. coli O157:H7 isolates, which did not carry stx2 were obtained from the faecal samples. Forty seven positive Stx2 antibody samples were obtained in the milk samples, whereas the Stx2 antibody was not detected in the farm where the E. coli O157:H7 isolates were obtained. The prevalence of O157:H7 is lower than the prevalence of the Stx2 antibody, and the isolates lack stx2. However, it is possible that the non-O157 STEC transfers the stx2 gene to O157:H7 horizontally, and makes O157:H7 become more virulent.

15

Isolating and identifying organic acids from Portulaca oleracea and determining their anti-cyanobacterial activity

72%

Wang H., Zhang L., Wang Y.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 1

We explored the possibility of using allelochemicals from terrestrial plants to solve the problem of cyanobacterial blooms in eutrophicated shallow lakes. The highly effective inhibitory allelochemicals of purslane (Portulaca oleracea L.) and purslane seeds were extracted and purified through a series of procedures, and the chemical components of the purified extracts were analyzed by gas chromatographymass spectrometry (GC-MS). The extracts contained 12 fatty acids and three phenolic acids, of which nine compounds were found to be inhibitors of harmful cyanobacteria. The inhibitory ratios of the purified extracts of purslane and purslane seeds on M. aeruginosa were 97.4 and 81.6%, respectively, when the cyanobacterial were exposed under 15.0 mg/L of extracts. The purified extracts exhibited significant anticyanobacterial activities, the organic acids that may contribute to the allelopathic effects of the purslane and purslane seeds on M. aeruginosa.

16

Persulfate oxidation for the remediation of petroleum hydrocarbon-contaminated soils

72%

Wu H., Sun L., Wang H., Wang X.

Polish Journal of Environmental Studies

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2016

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tom 25

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nr 2

The feasibility of total petroleum hydrocarbon-contaminated (TPH) soil remediation was studied using persulfate oxidation. Factors tested included type of activator, persulfate concentration, number of persulfate applications, and reaction time. Probe chemicals were used to study effi ciency of the persulfate oxidation mechanism. The best activation method used Fe2+, which achieved 40.8% TPH degradation at 24 h with an initial TPH concentration of 14,432.5 mg/kg. For alkaline (high pH) and hydrogen peroxide activation treatments, TPH degradation effi ciencies were 35.2% and 21%, respectively. Thermal activation effi ciency was relatively low (15.6%). Kinetic experiments demonstrated that the oxidation reaction was substantially completed within 60 min. A one-time addition of persulfate was superior to multiple applications. The addition of probe compounds produced sulfate radicals, hydroxyl radicals, and reductants. The results indicate that activated persulfate is reasonably effective for remediation of TPH-contaminated soils.

17

Isolation and degradation characteristics of a cold-resistant nitrobenzene-degrading strain from river sediments

72%

Qiu L., Wang H., Guo Y.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 3

18

Advantages of small-seeded species in forests: win-win for temporal and spatial dispersal among seeds in Rhododendron

72%

Wang H., Lai X., Zhang Y., Chen H.

Polish Journal of Ecology

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2019

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tom 67

|

nr 1

There is little consistent evidence for evolutionary associations from seed production to seedling fitness. In this study, 20 species of Rhododendron genus were selected from subtropical forests of southwest China; the life-history functional traits such as the seed size, seed number per twig, germination traits, dispersal and seedling survival were investigated, the ecological relationships among them were tested and the advantages of small-seeded species were discussed. The results show that the quantitative parameters of germination, such as the germination rate, potential, and index are positively correlated with seed number produced per stem, while temporal parameters, such as germination peak timing and duration are positively correlated with seed size. The two results indicate that different Rhododendron species with different seed sizes and relative seed number can deal with varying environments according to spatial dispersal or time dispersal, respectively, and the advantages of smaller seeds in the aspect of sprouting vigour, dispersal ability and seedling survival may lay the foundation for the establishment of small-seeded plants in the forest.

19

Structural diversity closely associated with canopy species diversity and stand age in species-poor montane forests on Loess Plateau of China

72%

Liu N., Wang H., Nan H.

Polish Journal of Ecology

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2017

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tom 65

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nr 2

High species diversity is often accompanied with and supported by a diversified stand structure in species-rich natural forests. However, the relationship between species diversity and stand structural diversity is less examined in species-poor forests. In montane forests on Loess Plateau of north-central China in a semi-arid climate zone, canopy species diversity and vertical structure of 57 broadleaves, conifer and mixed stands, with varying stand ages and site conditions, were randomly sampled. Canopy species diversity was represented by Shannon's index (H'). Stand structural diversity was represented by two indices respectively, i.e. coefficients of variation of diameter measurements at breast height (CVdbh) and Shannon's index of diameter classes (H'dbh). Structural equation models (SEMs) were constructed to explore multiple relationships between stand structural diversity and canopy species diversity, stand age and elevation. Both stand structural diversity indices increased directly with H' and stand age. However, indirect positive effect of stand age via increasing H' was only significant on CV. H'dbh provided positive feedback on H', while effect of stand age was only indirect via increasing structural diversity. Elevation significantly affected coefficients of variation of diameter, which was probably a sampling effect due to narrow distribution of broadleaves-conifer stands in altitudinal range. In conclusion, the results showed that stand structural diversity and canopy species diversity and stand age are closely associated with the species-poor montane forests like these on Loess Plateau of north-central China.

20

Addendum to “Sea spray aerosol flux estimation based on long-term variation of wave statistics”: estimation based on long-term variation of wind statistics

72%

Myrhaug D., Wang H., Holmedal L. E.

Oceanologia

|

2016

|

tom 58

|

nr 2

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