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The study was carried out from 1983 till 1989 in 21 parks in Warsaw. 1335 nest-boxes were inspected (3758 nest-boxes x years). There were 5 types, all made of wood: - A - dimensions: bottom 13 x 13 cm, height from bottom to lower edge of entry hole 15 or 20 cm, diameter of hole 3.3 cm; - A₁ - with the hole diameter 2.7 cm (other dimensions as for type A); - В - respective dimensions: 15 x 13 cm, 18 cm and 4.7 cm; enlarged В nest-boxes (Bm) -15 x 15 cm, 25 cm and 4.7 cm; - D -19 x 17 cm, 25 cm, 8.5 cm; - semi-open nest-boxes - 10 x 10 cm, 4 cm. The boxes were inspected once a season. During the 7 years of the study 1638 boxes were occupied (44% - in particular years from 32 to 52%). 11 bird species nested in the nest-boxes (Table 3), four of which - Passer domesticus, Passer montanus, Sturnus vulgaris and Parus caeruleus commonly did so. The first three occured in most of the areas investigated and one of them was usually the dominant species in the nest-boxes. Parus caeruleus was quite often to be found nesting in nest- boxes, whereas Partis major did so only infrequently- the opposite of the situation in woodland. The fewest species were recorded in young parks and in those visited by large numbers of people (SZ, MO, ST, SBM, UJ). Some species clearly preferred particular nest-box types (Fig. 2). Exceptionally high was percentage of enlarged В boxes used by Sturnus vulgaris (78%). However, only a small percentage of ordinary В and D nest-boxes were used. Besides bird broods, wasp and hornet nests were sometimes found, usually in types A. 5 squirrels nests were found (in type D boxes), and once, a bat was met in an A₁ nest-box. The study shows that certain nest-boxes were clearly preferred by birds, and that others were avoided (Table 5). Some of the factors that could have affected this behaviour were investigated: the density of trees, the extent of the nest-box's concealment in the tree crown, the direction which the entry hole faced, the box's inclination, cleaning of the boxes and their density. The following relationships were discovered: Passer domesticus preferred a low tree-density (16 and 28% occupation an a two-point scale; sample of 328 broods); Passer montanus avoided sites with a low density of trees (16% and 8% on a two-point scale; sample of 138 broods); Sturnus vulgaris and Passer montanus avoided nest-boxes hidden in tree crowns (14% and 24% respectively on a two-point scale and 4%, 14% and 15% on a three-point scale; 107 and 138 broods), for which Parus caeruleus displayed a preference (43%, 11% and 14%; 75 broods). Parus caeruleus also avoided nest-boxes from which old nests had not been removed (sample of 24 broods, one case of a brood in a box containing an old nest). That species did not nest again (with one exception) at a distance of less than 30 m from its nearest conspecific neighbour. Fewer nest-boxes tilting backwards (24%, N = 37) were occupied than nest-boxes fixed upright (44%, N = 1671) and tilting forwards (60%, N = 10).
The study was carried out in 7 parks in Poznań in 1987-1990, and in 2 parks from 1979 till 1990. P. Kozłowski's results from Warsaw with large nest-boxes for ducks and owls are included. The study had a threefold objective: - to assess the use made by birds of traditional types of boxes (A, B, D and semi-open P) in an urban environment; - to test new types of nest-boxes, hitherto untried in Poland; - to provide a basis for a comparison with the results of similar studies in Warsaw. In Poznań 2635 nest-boxes x years were inspected (from 145 to 350 annually) -2262 of traditional types (A, B, D and semi-open - type P) and 373 of large types for ducks and owls (Tabs. 1 and 2). The data in Table 3 are taken from 64 inspections of large nest-boxes in Warsaw. Description of 8 types of nest-box were inspected in Poznań: types A and B, built from sandwust-concrete, of sizes: - A - bottom 12 x 12 cm, height from bottom to lower edge of hole 15 cm, diameter of hole 3.3 cm; - В - 13 x 13 cm, 17.5 cm and 4.7 cm respectively; the other types of nest-boxes were made of wood: - D - bottom 15 x 15 cm, height from bottom to lower edge of hole 25 cm, hole diameter 8.5 cm; - P - semi-open, with respective dimensions 10 x 10 cm and 4 cm; - E - 23 x 23 cm, 55 cm, 15 cm; - K - 23 x 23 cm, height 79 cm (no roof); - "Anas" (doghouse) - 60-70 x 40-50 cm, height 40-50 cm. with partition half-way along and an entry hole 25-35 cm in diameter placed towards one and of the longer side; - "Certhia" type imitating a cervice - dimensions 25 x 12 x 3 cm with 3 x 5 cm hole (Fig. 1). During the entire study period in Poznan, 1528 nest-boxes were occupied by birds, i.e. 58% (from 36 to 84% in particular years). Broods of 13 species in nest-boxes were recorded (Tab. 4): Sturnus vulgaris and Passer montanus being the most frequent (684 and 501 occupied nest-boxes respectively). Parus major and Anas platyrhynchos were also numerous (195 and 116 respectively). Only 1 Passer domesticus nest was found in a box in Poznań, which is in stark contrast to the situation in Warsaw, where it is the most numerous species nesting in boxes. Phoenicurus phocnicurus, Ficedula hypoleuca and Erithacus rubecula were also more numerous in Warsaw. These differences were mainly due to the different nature of the parks in which the nest-boxes had been put up in the two cities. Also in contrast to Poznań, Parus caeruleus were more frequent box-nesters than Parus major in Warsaw. A significant factor here was the putting up of A₁ boxes in Warsaw. These have a smaller hole and are therefore preferred by the Parus caeruleus. Percentage occupation in Poznań was highest in A, В and D boxes (71,74 and 72% respectively). Occupation of other types did not exceed 13%, and not a single Certhia sp. took advantage of a „Certhia" nest- box. The large boxes for ducks and owls were rarely occupied. Only the "Anas" (doghouse) boxes and some of the type E nest-boxes located on islands in ponds were quite frequently used by Anas platyrhynchos (113 broods in 4 years).
Magpie in Warsaw is sedentary and ubiquitous species inhabiting all types of urban habitats. This study (1986-1990) estimated the breeding population in the entire area of Warsaw (485km²) at 6-8 p./km², and in winter (on the area of plots 245km²) — at 11-17 ind./km². The frequency of distribution in both seasons was 92-93% in the whole area 92-93%, and 100% in the inner city plots. Density in the inner city (52km² —19 p./km², in winter — 29 ind./km²) was 3 times higher than in the outer urban zone (150km² — 7 p./km², in winter 54km² 11 ind./km²) and 4-5 times higher than in outskirts (283km² — 5 p./km², in winter 139km² — 6 ind./km²). Density of population in winter decreased on outskirts and increased in inner city areas. The highest abundance was in green riverside belt (24 p. and 38 wintering ind./km²), in housing estates (21-25 p., 32 ind./km²) and in city parks (20-23 p., 36-37 ind./km²). The lowest was in peripheral forest parks and agriculture areas (2-4 p., 4-7 ind./km²). Colonization of the inner Warsaw by Magpie began in 1950-ties. Since then its population in this area (52km²) increased from few pairs to 800-1200 pairs. It reached the position of subdominant in the bird community, and its success is probably most dynamic among all species of the avifauna of Warsaw.
Seasonal changes in lipid droplet size and lipid peroxidation in the brown adipose tissue (BAT) of wild bank voles were examined. In addition, a role of photoperiod in these changes was studied; bank voles were held from the birth under long photoperiod (LP) for 12 weeks, and then half of them was transferred to short photoperiod (SP) for 6 weeks and another one remained under LP. In the wild bank voles the absolute BAT weight was seasonally constant, while the significant differences in the lipid droplet size were observed. The smallest lipid droplets (mean, 11 μm2) were seen in winter; they increased by 30 % in spring and reached the highest size (24 μm2) in summer. Lipid peroxidation in the BAT did not differ significantly between the seasons, although high intraseason variation of this process was noted. The laboratory experiment revealed that the size of lipid droplets was determined by photoperiod; SP induced 13-fold decrease, and continuous exposure to LP brought about a further 2.5-fold increase in the size of lipid droplets. Conversely, a significant decrease in lipid peroxidation was seen in LP bank voles in comparison with the SP animals. The data indicate that short photoperiod is responsible for the small size of lipid droplets in the BAT of bank voles during winter, which may be a necessary requirement for high thermogenic capacity of the tissue. Photoperiod appears also to affect lipid peroxidation in the BAT of these animals.
The study covered 5 species occupying nest-boxes in urban parks most frequently: Passer montanus, P. domesticus, Sturnus vulgaris, Parus major and P. caeruleus. The main objective of the study was to find out whether nest-boxes in the conditions peculiar to urban parks enabled these species to breed successfully there. The assessment was based on a comparison of these results with those, given in the literature, from farmland and woodland near the two cities. This project is part of a wider programme of studies into the use of nest-boxes in parks in Warsaw and Poznań (Luniak 1992). Methods. Small and medium-sized nest-boxes were used in this study - wooden ones in Warsaw, sawdust-concrete ones in Poznań. Some of the material (Tab. 1) was collected while other studies were being conducted. These required frequent and lengthy nest inspections which exposed the parent birds to considerable stress. The following terms and means of calculating breeding parameters are used: „Fledged pulli" - successfully reared nestlings. Means of „Hatched pulli" and „Fledged pulli" were calculated with respect to the number of broods with cluthes. Brood losses refer to the loss of whole cluthes or all the nestlings in the nest. Productivity (pull./nest/year) was calculated as the sum of fledglings from all broods referred to the number of nests (pairs) with first and delayed clutches. Study areas. In both cities the studies were carried out in parks within the highly urbanized zone. In Warsaw, the bulk of the material on Passer montanus and P. domesticus was collected in 3 parks (Table 1), the remainder in over a dozen others. In Poznań, the study covered 2 parks. In all the parks there was quite a wide variety of predators and species destroying broods - domestic cats, Martes spp., Sciurus vulgaris, Strixaluco, Corvus monedula and Pica pica. With the exception of one area in Poznań (Ogród Dendrologiczny - Tree Garden), visited by large numbers of people. Breeding results. Table 2 compares the breeding parameters of the 5 species studied in the parks with those given in the literature for the farmland and woodland near Warsaw and Poznań. Tables 3-7 give the detailed breeding results for the 4 species in the parks. Conclusions. - The results of breeding in nest-boxes in Warsaw and Poznań parks were within the ranges typical for the species in question. - In none of the 5 species were the breeding results (clutch size, productivity) in the parks distinctly worse than those reported in the literature for nearby non-urban areas. The productivities of Passer montanus and Sturnus vulgaris in Poznań were slightly lower than those outside the city; that of Parus major in Poznań was higher. - No significant differences in breeding results between the two cities were found, and there were no regular differences, as well, between breeding results in wooden boxes (used in Warsaw) and sawdust-concrete ones (used in Poznań). - Breeding results were not visibly affected by direct human interference, the presence of large numbers of people near the nest-boxes, predation by raptors and nest robbers or interspecific competition. But the domination of Sturnus vulgaris and Passer montanus (and probably P. domesticus as well) limited possibilities of occupying boxes by Parus major and P. caeruleus. Site and intrapopulational factors were probably more important as regards limitation of productivity. - The frequent and lenghty nest inspections more often led to the loss of entire clutches/broods.
To control the animal behaviour in a farrowing pen a device consisted of optical switch, electric heater-blower and an air-jet steering the air stream in determined place of farrowing pen, was designed and built. The blower was being switched on at the moment of sow standing and disconnected after the sow lying down. The optimum height of air-jet in farrrowing pens of the Strzeszyn type is 65 cm. Switching on the blower did not cause any negative form of sows’ behaviour and dislodging of the piglets was very effective, what prevented the acccidents of piglets’ crushing. The reliability of optical switch functioning was found satisfactory.
The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be done even in a reaction volume as low as 1 jxl. The recommended volume, 2.5 jil or 5 jil, allows significant savings in the laboratory budget especially for laboratories which use PCR frequently and on a large scale.
Numerous human transcripts contain tandem repeats of trinucleotide motifs, the function of which remains unknown. In this study we used the available gene expression EST data to characterize the abundance of a large group of these transcripts in different tissues and determine the mRNAs which had the highest contribution to the observed levels of transcripts containing different types of the CNG repeats. A more extensive characteristics was performed for transcripts containing the CUG repeats, and those encoding the repeat-binding proteins. The scarcity of double-stranded CUG repeats as well as various proportions of the single-stranded and double-stranded CUG repeat-binding proteins were revealed in the studied transcriptomes. The observed correlated levels of transcripts containing single-stranded CUG repeats and of proteins binding single-stranded CUG repeats may imply that in addition to transcripts which only provide binding sites for these proteins there may be a substantial portion of the transcripts whose metabolism is directly regulated by such proteins. Our results showing a highly variable composition of triplet repeat-containing transcripts and their interacting proteins in different tissues may contribute to a better understanding of the mechanism of RNA-mediated pathogenesis in triplet repeat expansion diseases.
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