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Investigations were carried out in 1996-1998 on experimental plots sown with different oat cultivars and lines. Symptoms of the fusarium panicle blight developed on 0.5-15% of oat plants. Results of this studies showed that fusarium blight of oat panicles is widespraed in Lublin region. Fusarium avenaceum and F. poae proved to be the cause of the disease. Also F. culmorum, F. crookwellense, F. equiseti, F. oxysporum, F. sporotrichioides were isolated from infected panicles.
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63%
Investigations on Fusarium head blight of rye were carried out in the years 2005-2007 on 10 production fields in the Lublin region. The percentage of heads showing the fusariosis symptoms in the years 2005-2007 ranged from 0 to 7%. Mycological analysis of kernels and chaff obtained from heads with Fusarium blight (scab) symptoms showed that Fusarium avenaceum, Fusarium culmorum and Fusarium sporotrichioides were the largest threat to heads of this cereal. The species of Fusarium poae and Fusarium crookwellense were also isolated from infected rye heads. The dominance of particular species in infecting rye heads was determined by weather conditions.
Investigations were carried out in 1996-1998 in the experimental fields in Zamość region. The following ten lines of oats were tested: CHD 894, CHD 1095, CHD 1236, CHD 1607, CHD 1653, CHD 1692, STH 2293, STH 2393, STH 2494, STH 2694. The seedlings which showed symptoms of either root and sheath nectosis, or rolling stems ranged form 9 - 36 ad 9 - 70 % of all seedlings tested, respectively. Results of mycological analysis of seedlings showed that F. avenaceum and F. culmorum were most frequently represented on infected parts (43% ad 42% of all Flusarium spp. isolates respectively), and also they were the most frequently obtained trom stems wit h necrotic stripes (24 % and 46 % of all Fusarium spp., respectively). Apart from F. avenaceum and F. culmorum there was some F. crookwellense found every year on lower internodes.
The present study was carried out in the period 2006- 2008 based on an experiment established in 2005. The study evaluated the effect of conservation and plough tillage as well as of four catch crops on the level of infection by fungal pathogens of the stem base and roots of the spring wheat cultivar ‘Zebra’ grown in monoculture. The species composition of fungi colonizing the stem base and roots of spring wheat was determined. The split-plot design of the experiment set up on rendzina soil included plough tillage and conservation tillage with autumn and spring disking of catch crops. The experiment used four methods for regeneration of the spring wheat monoculture stand using the following: undersown red clover and Westerwolds ryegrass crops as well as lacy phacelia and white mustard stubble crops. Plots without catch crops were the control treatment. Red clover and Westerwolds ryegrass catch crops as well as lacy phacelia and white mustard stubble crops had a significant effect on the decrease in the stem base and root infection index of spring wheat compared to the control without catch crops. The disease indices in the tillage treatments under evaluation did not differ significantly from one another. The stem base and roots of spring wheat were most frequently infected by fungi of the genus Fusarium, with F. culmorum being the dominant pathogen of cereals. Compared to conservation tillage, in plough tillage the pathogenic fungus Bipolaris sorokiniana was not found to occur on the stem base and roots. The Westerwolds ryegrass catch crop promoted the occurrence of F. culmorum, both on the stem base and roots of spring wheat.
Zinnia elegans is an annual plant recommended for cultivation for cut flowers as well as flowerbeds. This ornamental plant can be infected by: viruses, bacteria, phytoplasms and by a number of fungi species. Presented studies were conducted in 2006 in three localities of the Lublin region: in the vicinity of Zamość, near Krasnystaw and near Opole Lubelskie. Those studies included three cultivars of Zinnia elegans Jacq.: Golden Dawn, Lawa, Scarlet Flame as well as the mixed material of the cultivars belonging to the dahlia group. Six weeks after the sowing, the seedling’s healthiness was assessed. The proportion of seedlings with disease symptoms ranged from 7% to 44%. The main cause of root infection at the seedling stage proved to be the species of F. equiseti and S. sclerotiorum. Considerable amounts of B. cinerea and F. culmorum and A. alternata were also detected from diseased seedlings. Studies on the susceptibility of analyzed cultivars and the mixed material of elegans zinnia to infection by B. cinerea and F. avenaceum F. culmorum and F. equiseti were conducted in a growth chamber. Results obtained from this experiment confirmed considerable harmfulness of the species B. cinera and F. equiseti towards the seedlings of elegans zinnia, which can be reduced introducing less susceptible varieties to the cultivation. However, none of the studied varieties of elegans zinnia, only its mixed material, showed such properties.
In Poland, cultivars belonging to three species of Tagetes are grown: T. erecta, T. patula, T. tenuifolia. Presented studies were conducted in 2015 in two localities of the Lublin region. Those studies included cultivars of Tagetes spp.: T. erecta (cv. ‘Alaska’, ‘Hawaii’, ‘Mann Im Mond’), T. patula (cv. ‘Bolero’ and ‘Carmen’) and T. tenuifolia (cv. ‘Lemon Gem’ and ‘Lulu’). Six weeks after the sowing and in flowering stage of analyzed marigold cultivars, the plants healthiness was assessed. The mean values of the disease index ranged from 16.25 to 29.0. The main cause of root infection at the seedling stage proved to be the species of Fusarium and Rhizoctonia solani. Proportion of plants with disease symptoms in flowering stage ranged from 1.25 to 5.5%. Regardless of the place of cultivation, species: A. alternata, F. culmorum and F. equiseti were the most often isolated from diseased plants in the flowering stage. Studies on the susceptibility of analyzed cultivars of marigold to infection by Fusarium culmorum, F. equiseti and F. sporotrichioides were conducted in a growth chamber. Significantly, the lowest value of the disease index of cultivar ‘Bolero’ (T. patula) allows to accept that cultivar as the least susceptible to infestation by Fusarium spp. under conditions of controlled temperature and humidity.
Field experiments were performed during three years (1999, 2000 and 2001) to study fusariosis on oat panicles. The percentage of panicles with fusariosis symptoms ranged from 0,5 to 6%. Fusarium panicle blight was caused mainly by Fusarium avenaceum and F. poae. The experiment with panicles artifical infection of 12 genotypes of oat was performed in 2001. The panicles of oat were inoculated with F. poae strain nr 37, which caused a reduction in yield by 37%. In the infected kernels the following toxins were detected: nivalenol (from 0.06 to 2.18 mg·kg⁻¹), deoxynivalenol (from 0.02 to 0.47mg/kg⁻¹), T-2 toxin (from 0.02 to 0.71 mg·kg⁻¹), HT-2 toxin (from 0.03 to 0.59 mg·kg⁻¹), scirpentriol (from 0.06 to 1.98 mg·kg⁻¹).
The present study was carried out in the years 2010–2012 in the fields of the Strzelce Plant Breeding Company Ltd., belonging to the Plant Breeding and Acclimatization Institute in Radzików, and it included 39 oat genotypes. At the six-week seedling stage, the percentage of plants with root and leaf sheath necrosis symptoms was evaluated. In 2010 the percentage of seedlings with disease symptoms ranged from 6.5% to 25%, in 2011 it ranged from 17% to 34.5%, whereas in 2012 from 10% to 25%. In 2010 the disease index ranged from 1.4 to 5.7, in 2011 from 4.5 to 8.8, while in 2012 it was between 2.0 and 5.4. Mycological analysis showed that large numbers of Fusarium spp. colonies were obtained both from the roots and leaf sheaths. Isolates of these fungi accounted for 63.48% of the total fungi isolated from seedlings. Seedlings grown under the conditions of central Poland were damaged by the species F. culmorum, F. avenaceum, and F. solani. The investigation of the susceptibility of 15 oat genotypes to infection with two Fusarium graminearum strains – Tz 56 and Tk 235 – was carried out under growth chamber conditions at a temperature of 22–23oC and relative air humidity of 85%. The F. graminearum strain Tz 56 proved to be the most pathogenic to seedlings of the breeding lines STH 0.9403 and POB 1316/08, for which the disease index was 80.5 and 75.5, respectively. The lowest pathogenicity of the a.m. strain was recorded in the case of the genotype DC 1832/05, for which the disease index was 26.5. The F. graminearum strain Tk 235 proved to be the most pathogenic to the genotypes STH 0.9403 and STH 0.9423, for which the disease index was 70.5 and 70.0, respectively, whereas this strain was least pathogenic to the breeding line DC 2112/05, in the case of which the disease index was 25.5.
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Fungi occurred on turfgrasses in lawn maintenance

51%
In recent years there has been an increasing interest in lawns and their appearance. Diseases affecting lawns, of which the causative agents can be various pathogens, reduce their quality. The study was conducted in two urban centers in the region of southeastern Poland – in the years 2006 and 2007 in Lublin and in 2007 in Zamość, both in sunny and in partly shaded places. The species composition of the grass sward and the severity of leaf infection were determined as well as mycological analysis of infected plants was carried out. In the case of the lawns in Lublin, the mean values of the disease indices for the leaves of the investigated turfgrasses were from 53.75 to 83.00 in the spring of 2006, while in spring of 2007 from 6.75 to 29.00. The mean values of the leaf disease indices determined in autumn of 2006 were from 16.00 to 86.25, whereas in autumn of 2007 from 4.50 to 21.25. The mean values of the disease indices for leaves of the turfgrasses studied in Zamość were from 24.50 to 48.25 in the spring of 2007, while in the autumn of that year they ranged from 28.00 to 54.75. Microdochium nivale was a great threat to turfgrasses in lawn culture in spring, whereas the cause of leaf spot of turfgrasses in the autumn were species B. sorokiniana and D. siccans. As far as the lawns in both cities are concerned, species of the genus Fusarium, in particular Fusarium culmorum, had a high percentage in root infection both in the spring and in the autumn.
Investigations were carried out in 2007-2009 on the plots of the Felin Experimental Station belonging to the University of Life Science in Lublin. The studies comprised two cultivation lines of durum wheat (Triticum durum L.): STH 716 and STH 717, as well as the ‘Tonacja’ cultivar of common wheat (T. aestivum ssp. vulgare L.). Two levels of chemical protection were applied in the cultivation: minimal and complex protection. Infection of wheat roots and stem bases was recorded in each growing season at hard dough stage (87 in Tottman’s scale, 1987). After three years of study, the mean disease indexes for the analyzed wheat genotypes in the experimental treatment with minimal protection were 31.13, 30.43 and 38.83 for, respectively, the ‘Tonacja cultivar’ and the cultivation lines of T. durum STH 716 and STH 717. In the experimental combination with complex protection, after three years of study the disease indexes ranged from 25.26 (T. durum STH 716) to 30.83 (T. durum STH 717). The results of mycological analysis of diseased plants showed that Fusarium spp., especially F. culmorum, F. avenaceum as well as Bipolaris sorokiniana and Rhizoctonia solani, caused root rot and necrosis of wheat stem bases. The analyzed chemical protection levels did not significantly influence grain yield of the investigated genotypes of T. aestivum and T. durum.
One of the reasons lowering the esthetic appearance of a lawn are diseases caused by fungi. Studies were conducted on the sowing material of fifteen turfgrasses belonging to five species. Samples of seeds of the studied cultivars were from the harvest of 2007 and 2008. 573 fungi isolates belonging to 28 species and non-sporulating forms were obtained as a result of the mycological analysis during the two years of the experiment. In each year of studies, the greatest number of colonies belonging to Alternaria alternata. Within the group of pathogenic fungi: Fusarium culmorum, F. avenaceum, F. equiseti, F. solani, F. crookwellense, F. graminearum F. sporotrichioides, B. sorokiniana, D. siccans and Rhizoctonia solani were obtained. Studies on susceptibility of the seedlings of 8 cultivars of 4 grass species to infection by D. avenae No. 2, D. siccans No. 8 and B. sorokiniana No. 69 were conducted in a growth chamber. The statistical analysis of disease indexes for plants that grew in the experimental combination with artificial infection of the subsoil with above-mentioned strains as compared to the control indicated significant differences in all studied cultivars. Strain D. siccans No. 8 proved to be the most pathogenic towards cultivar Info of perennial ryegrass (92.20), and B. sorokiniana No. 69 towards wood bluegrass Pinokio cultivar (97.75). In the experimental combination with D. avenae the mean values of disease index ranged from 12.25 (Pinia) to 84.00 (Info).
Investigations were carried out in 1996 - 2000 in the Experimental Station of Cultivars Evaluation in Uhnin. Disease symptoms were recorded twice - in the seedling stage (18 in the Tottman's scale) and in milk ripe stage (77 in the Tottman's scale) of oat. The seedlings with root and sheath necrosis or plants with diseased stem base were obtained every year. The percentage of diseased seedlings ranged from 6.0 to 39.5, and percentage of older plants with necrotic stripes on lower intemodes ranged from 11.5 to 50.0. Results of mycological analysis of diseased plants showed that Fusarium spp., especially F. avenaceum and F. culmorum were isolated the most frequently, F. avenaceum was obtained in the seasons with different weather conditions. This indicates great tolerance of this fungus to temperature and humidity. In the seasons with high temperature F. culmorum was predominant. This species also can be tolerant to changing weather conditions. Warm and wet weather was favourable for oat infection by Bipolaris sorokiniana. Rhizoctonia solani was isolated from plants in milk ripe stage in each vegetation seasons, and from seedlings in years 1998 and 1999.
Field observations of oat panicles carried out in the fields of Danko Plant Breeding Company in the period 2006–2007 and in the fields of Strzelce Plant Breeding Company in 2008 showed the occurrence of panicles with Fusarium head blight symptoms in each growing season. In 2006 the percentage of such panicles ranged from 0.25 to 1.5%, in 2007 from 2.0 to 9.0%, whereas in 2008 from 0.5 to 8.0%. The species Fusarium poae was the main causal agent of Fusarium head blight. A study on inoculation of panicles of 12 genotypes of oats with Fusarium poae strain no. 35, which was conducted in 2008 in experimental fields near the city of Zamość, determined the number of kernels per panicle, grain yield from 40 panicles (4×10 panicles), and 1000-kernels weight (TKW) after the harvest of the crop at full grain maturity. Compared to the control, the lowest reduction in the number of kernels per panicle was found in the case of the cultivar ‘Krezus’ (88.69% of the control), while the highest one in ‘Szakal’ (22.46% of the control). As a result of inoculation of panicles with F. poae, the breeding line STH 8107 was characterized by the lowest decrease in kernels yield (69.76% of the control), whereas the highest decrease was found in the breeding line CHD 1430/02 (14.26% of the control). Compared to the control, the lowest reduction in TKW was observed in the breeding line STH 8107 (96.46% of the control), whereas the highest one in the breeding line CHD 1430/02 (45.06% of the control). The presence of secondary metabolites of F. poae and group A trichothecene compounds: HT-2 toxins (from 0 to 0.013 mg × kg-1), diacetoxyscirpenol (DAS) (from 0 to 0.002 mg × kg-1), T-2 tetraol (from 0.001 to 0.014 mg x g-1), and scirpentriol (from 0.008 to 0.074 mg × kg-1), was found in infected oat kernels. Group B trichothecenes: nivalenol (from 0 to 0.157 mg × g-1), deoxynivalenol (DON) (from 0 to 0.127 mg × kg-1) as well as its acetylated derivatives: 3-AcDON (from 0 to 0.059 mg × kg-1) and 15-Ac DON (from 0 to 0.288 mg × kg-1), were also present in oat kernels obtained from panicles artificially infected with Fusarium poae.
Serum fatty acid profile was studied in 178 adults, including 116 vegetarians on various diets (lactovegetarian, lactoovovegetarian, ovovegetarian and semivegetarian) and 62 on normal diet. All subjects were divided into two age groups, so that the mean ages in the groups of vegetarians and non-vegetarians were similar. It was found that in the vegetarian groups the values of PUFA and EFA (essential fatty acids) in serum were higher than in control non-vegetarians, and the differences were particularly evident in the groups aged over 26 years. Higher levels of PUFA and EFA in serum of vegetarians were connected mainly with higher levels of linoleic acid and arachidonic acid. The effect of vegetarian diets on serum fatty acids in human may be regarded as favourable.
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