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Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

100%

Fratrikova M., Bauer M., Jopcik M., Libantova J.

Acta Scientiarum Polonorum. Hortorum Cultus

2018

tom 17

nr 1

Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.

Expression pattern of Arabidopsis thaliana pollen- and embryo-specific promoter in transgenic tobacco plants

100%

Jopcik M., Matusikova I., Moravcikova J., Libantova J.

Acta Biologica Cracoviensia. Series Botanica

2014

tom 56

nr 1

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Scientiarum Polonorum. Hortorum Cultus

2 Jopcik M.

2 Libantova J.

1 Bauer M.

1 Fratrikova M.

1 Matusikova I.

1 Moravcikova J.

1 2018

1 2014

Wyniki wyszukiwania

Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

Expression pattern of Arabidopsis thaliana pollen- and embryo-specific promoter in transgenic tobacco plants