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Gene expression of RNAP II, JBP1 and JBP2 in Leishmania major exposed to antimonials, amphotericin B and paromomycin

100%

Ajamein V., Eslami G., Ahmadian S., Khamesipour A., Elloumi M.

Annals of Parasitology

2018

tom 64

nr 3

Cutaneous leishmaniosis (CL) is treated with pentavalent antimony (SbV) as a first-line drug, while amphotericin B and paromomycin are potential alternatives in antimonial- resistant isolates. However, the mechanisms of drug resistance remain unclear. The present study analyses the gene expression of RNA polymerase II (RNAP II) and J-binding protein 1 (JBP1), and J-binding protein 2 (JBP2) in Leishmania major after exposure to drugs in vitro. L. major (MRHO/IR/75/ER) promastigotes were exposed to various concentrations of glucantime, paromomycin and amphotericin B for 72 hours. The RNA was then extracted and used for cDNA synthesis. The expressions of JBP1, JBP2 and RNAP II were analysed using SYBR Green real-time PCR. No change in JBP2 or RNAP II expression was associated with amphotericin B, but JBP1 expression decreased with increasing drug concentration. Paromomycin had no effect on JBP2 expression, but a 13.5-fold increase in JBP1 was observed at 100 μg/ml, and a decrease in RNAP II expression at 25 and 50 μg/ml. Exposure to glucantime resulted in 1.4-fold lower JBP1 expression at 5 μg/ml, and 333.33- to 500-fold lower RNAP II at concentrations of 5 to 15 μg/ml. As Base J synthesis requires both JBP1 and JBP2, RNAP II (encoding RNA polymerase II) could reduce expression. However, RNAP II was not expressed in all groups, indicating that the genes associated with drug resistance may be regulated in other ways.

Evaluation of immune response against leishmaniasis in resistance C57 BL-6 mice immunized with liposomes containing autoclaved Leishmania major with BCG

100%

Sohrabi Y., Jaafari M. R., Mohammadi A. M., Eskandari S. E., Khamesipour A.

Cellular and Molecular Biology Letters

2005

tom 10

nr Suppl.

A survey on the adjuvant role of naloxone alone or combined with alum in vaccination against fasciolosis in BALB/c mice

72%

Azizi H., Mirzaeei H., Bagheri A., Bazi A., Khamesipour A., Yaghoobi H., Mirzapour A., Khatami M., Elikaee S.

Acta Parasitologica

2019

tom 64

nr 2

Ograniczanie wyników

1 Acta Parasitologica

1 Annals of Parasitology

1 Cellular and Molecular Biology Letters

3 Khamesipour A.

1 Ahmadian S.

1 Ajamein V.

1 Azizi H.

1 Bagheri A.

1 Bazi A.

1 Elikaee S.

1 Elloumi M.

1 Eskandari S. E.

1 Eslami G.

1 Jaafari M. R.

1 Khatami M.

1 Mirzaeei H.

1 Mirzapour A.

1 Mohammadi A. M.

1 Sohrabi Y.

1 Yaghoobi H.

1 2019

1 2018

1 2005

Wyniki wyszukiwania

Gene expression of RNAP II, JBP1 and JBP2 in Leishmania major exposed to antimonials, amphotericin B and paromomycin

Evaluation of immune response against leishmaniasis in resistance C57 BL-6 mice immunized with liposomes containing autoclaved Leishmania major with BCG

A survey on the adjuvant role of naloxone alone or combined with alum in vaccination against fasciolosis in BALB/c mice