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1

Odzwierzęce szczepy Staphylococcus aureus oporne na metycylinę (LA-MRSA) - obecny stan wiedzy

100%
Kizerwetter-Swida M., Plawinska-Czarnak J.
Medycyna Weterynaryjna
|
2017
|
tom 73
|
nr 02
A methicillin-resistant Staphylococcus aureus (MRSA) referred to as livestock-associated (LA-MRSA) has recently emerged in farm animals, particularly in pigs. Strains of this MRSA variant from Europe and North America mostly belong to clonal complex (CC) 398. Generally LA-MRSA cause asymptomatic colonization among pigs, but also in veal calves, broiler chickens, turkeys, and horses. People in contact with livestock animals are at high risk of asymptomatic colonization or infection with these bacteria. In previous years, the impact of LA-MRSA on human health was considered small. However, LA-MRSA has become more prevalent among people without direct livestock contact, especially in areas with a high density of pig production. As a result of horizontal gene transfer S. aureus CC398 strains are constantly evolving. The adaptive power of S. aureus to new hosts and acquisition of resistance to antibiotics may cause the emergence of new, more virulent clones. LA-MRSA has evolved from human-adapted methicillin-susceptible S. aureus CC398, which was proved by comparative genome analysis. The adaptation to livestock was associated with several genetic changes. The most worrying aspect of MRSA CC398 seems to be its ability to spread to humans. For this reason, continuous surveillance of further genetic changes is recommended.
2

Salivaricin B gene - its localization and RFLP analysis in two potentially probiotic Lactobacillus salivarius strains

100%
Kizerwetter-Swida M., Binek M.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The study has provided data on two Lactobacillus salivarius strains of poultry origin. The strains were investigated for the presence of genes encoding a bacteriocin: salivaricin B, and their localisation in chromosome or plasmid DNA. Specific primers were used to amplify a 224 bp fragment of salivaricin B gene (salB). RFLP analysis of PCR products revealed two DNA fragments of the predicted sizes upon digestion with Ndel and Xapl. Analysis with SspI allowed obtaining variability in two fragments in comparison to computer analysis of both strains, suggesting their divergency. It may be assumed that the amplified DNA fragments of salB gene share great, but not complete similarity to the previously described sequence of salB gene. Two examined strains showed different plasmid profiles; however bands of similar sizes were seen in both profiles. Genes responsible for salivaricin production are located on chromosomal DNA. Properties of these strains, in particular the presence of genes encoding bacteriocin production, imply that they may be used as potential probiotics for poultry.
3
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Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens

100%
Kizerwetter-Swida M., Binek M.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 1
This study was performed in order to isolate lactobacilli from chicken droppings and to select strains with the most promising probiotic properties. Lactobacillus strains were isolated from a flock of healthy laying hens. The first selection criterion was the ability to inhibit the growth of Salmonella Enteritidis. Then the tolerance to low pH and bile salt, the ability to coaggregate with pathogenic bacteria and hydrogen peroxide production were evaluated. Four isolates showing the best antagonistic activity against Salmonella Enetritidis were selected for further research. All isolates tested tolerated low pH and bile salt, likewise all produced hydrogen peroxide. They efficiently coaggregated with C. perfringens and relatively less with E. coli. Isolate 03’04 displayed above-average results in all criteria, thus it is considered as a potential probiotic for chickens, and will be further evaluated for health promoting effect in animals. The results presented in this study confirm the strain specific probiotic properties and prove the probiotic potential of isolate 03’04. Strong antagonistic properties against C. perfringens exhibited by certain Lactobacillus strains indicate the possibility to use them as a component of probiotic supplement in necrotic enteritis of poultry.
4

Adhesion properties of Lactobacillus strain of poultry origin and characterisation of its antibacterial product

100%
Kizerwetter-Swida M., Binek M.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 4
5

Gronkowce izolowane od zwierząt jako źródło genów kodujących wielolekooporność na antybiotyki o krytycznym znaczeniu dla zdrowia publicznego

100%
Kizerwetter-Swida M., Plawinska-Czarnak J.
Medycyna Weterynaryjna
|
2017
|
tom 73
|
nr 10
Antimicrobial resistance (AMR) is a global public health issue. Multidrug resistance (MDR) genes that confer resistance to antimicrobials from different classes are of particular importance in the spread of AMR. Moreover, some of these MDR genes are involved in resistance to critically important antimicrobial agents used in human and veterinary medicine. Staphylococci isolated from animals and humans harbor a wide range of resistance genes, including MDR genes. Location of MDR genes on mobile genetic elements facilitate the exchange of these genes between staphylococci of animal and human origin. The emergence of resistant Staphylococcus spp. is probably linked to therapeutic or prophylactic antimicrobial use through not only direct selection of the corresponding resistance, but also indirect selections via cross-resistance and co-resistance. Judicious use of antibiotics and the knowledge of the genetics of MRD genes and other resistance genes is indispensable to counteract further dissemination of staphylococcal MDR genes.
6

Protective effect of potentially probiotic Lactobacillus strain on infection with pathogenic bacteria in chickens

100%
Kizerwetter-Swida M., Binek M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
The probiotic potential of a Lactobacillus salivarius 3d strain isolated from chicken faeces was assessed in one day old chickens. Lactobacillus salivarius 3d was administered per os at a concentration of 10⁸ cfu in 100 μl of PBS. The chickens were then challenged with pathogenic bacteria: Salmonella Enteritidis, Campylobacter jejuni and Clostridium perfringens. Samples of caecal contents and livers were collected after 1, 2, 3, 7 and 14 days after infection. Lactobacilli and pathogenic bacterial cell counts were determined in the samples. This study showed that L. salivarius 3d reduced the number of Salmonella Enteritidis and Clostridium perfringens in the group of chickens treated with Lactobacillus. Therefore it may be concluded that L. salivarius 3d may be used as a potential probiotic for chickens.
7

Zatrucie jadem kielbasianym - problem wciaz aktualny

100%
Kizerwetter-Swida M., Binek M.
Postępy Mikrobiologii
|
2010
|
tom 49
|
nr 2
75-85
8

Bacterial microflora of the chicken embryos and newly hatched chicken

100%
Kizerwetter-Swida M., Binek M.
Journal of Animal and Feed Sciences
|
2008
|
tom 17
|
nr 2
9

Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria

100%
Kizerwetter-Swida M., Binek M.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 4
Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not always satisfactory. ITS-PCR together with protein profile were found to be helpful in strain identification. Lactobacilli were tested for the inhibitory activity against selected strains of poultry enteropathogenic bacteria (Salmonella Enteritidis, Escherichia coli and Clostridium perfringens). Examined supernatants from Lactobacillus broth cultures demonstrated major antimicrobial activity against C. perfringens. Lower antimicrobial activity were observed against E. coli and Salmonella Enteritidis. The strongest inhibition effect were obtained using supernatant of Lactobacillus acidophilus strain 3D. Results received from this study confirmed that identification of Lactobacillus spp. is often tedious. Some isolates, which are in vitro antagonistic against enteropathogenic bacteria may be considered as potential candidates for poultry probiotics, especially in controlling necrotic enteritis caused by C. perfringens.
10

Meticylinooporne szczepy Staphylococcus intermedius wystepujace u psow jako potencjalny rezerwuar genu mecA

81%
Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.
Postępy Mikrobiologii
|
2009
|
tom 48
|
nr 4
235-242
11

Wkład mikrobiologii weterynaryjnej w budowę idei wspólnego zdrowia

81%
Binek M., Kizerwetter-Swida M., Chrobak-Chmiel D.
Postępy Mikrobiologii
|
2018
|
tom 57
|
nr 2
12
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The first description of gastric Helicobacter in free-ranging wild boar [Sus scrofa] from Poland

81%
Fabisiak M., Sapierzynski R., Salamaszynska-Guz A., Kizerwetter-Swida M.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
171-174
Specimens of gastric mucosa of 17 free-ranging wild boars (Sus scrofa) shot in the Central Poland during 2007/2008 hunting season were investigated for the presence of Helicobacter species. Histopathology, Helicobacter genus-specific 16S rRNA PCR, and DNA sequence analysis were employed. In PCR analysis the presence of Helicobacter's DNA was detected in one stomach. Obtained sequence analysis showed its relatedness to Helicobacter heilmannii type 2. In histopathology of the PCR-positive sample the presence of tightly coiled spiral bacteria was detected on the surface of the antral mucosa, in gastric pits and lumen of the upper parts of antral glands. Potential pathologic significance of the presence of Helicobacter in the stomach of free-ranging wild boars was obscured by the parasitic invasion-caused gastritis, and remains unknown.
13
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Problemy weterynaryjnej diagnostyki mikrobiologicznej dotyczącej zakażeń gronkowcowych

81%
Kizerwetter-Swida M., Chrobak-Chmiel D., Rzewuska M.
Życie Weterynaryjne
|
2018
|
tom 93
|
nr 06
14

Helicobacter sp. microorganisms do not alter proliferative activity of gastric epithelial cells in naturally infected swine

81%
Sapierzynski R., Fabisiak M., Kizerwetter-Swida M., Dolka I.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
In the presented study, an evaluation of influence of different Helicobacter species and gastritis on intensity of cellular proliferation in pyloric glands of pigs' stomach was performed. Samples of gastric antral mucosa obtained from 38 slaughtered pigs with known Helicobacter sp. and gastric inflammation statuses were stained with haematoxylin-eosin and immunohistochemically, for Ki67 antigen expression. Proliferative activity of epithelial cells was assessed by determination of: a ratio of proliferative zone length to gastric crypts length, an average percentage of cells showing Ki67 expression in proliferative zones of antral glands, and value of mitotic index in glands' proliferative zones. None of the comparisons revealed statistically significant differences between animal groups with or without gastric inflammation, as well as between groups with or without Helicobacter colonisation. Additionally, no statistically significant differences were found between the group of animals that were infected with Candidatus Helicobacter suis, and that with the stomach colonised by different species of Helicobacter microorganisms.
15

Kretki z rodzaju Brachyspira jako czynnik etiologiczny spirochetozy jelitowej ptakow

81%
Kizerwetter-Swida M., Rzewuska M., Binek M.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 08
883-886
Spirochetes Brachyspira spp. are found in large intestines of humans and animals. The following pathogenic species of Brachyspira are involved in avian intestinal spirochaetosis (AIS): B. alvinipulli, B. pilosicoli, B. intermedia and B. hyodysenteriae. There are also unclassified spirochetes isolated from birds, which are known as B. pulli. AIS may cause significant economical losses in poultry production. AIS seems to be a fairly widespread problem in laying hens and captive rheas. Chronical infections with a mild course of the disease are difficult to diagnose and only limited data concerning the prevalence, controlling and prevention of AIS are available.
16

Staphylococcus pseudintermedius – trudno rozpoznawalny patogen

81%
Kizerwetter-Swida M., Chrobak-Chmiel D., Rzewuska M., Binek M.
Postępy Mikrobiologii
|
2015
|
tom 54
|
nr 2
17
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Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

81%
Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius- specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.
18

Nasilenie i charakterystyka zapalnego nacieku komorkowego w blonie sluzowej zoladka swin przy zakazeniu Helicobacter sp.

81%
Sapierzynski R., Fabisiak M., Kizerwetter-Swida M.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 09
1102-1105
The aim of the study was to estimate the occurrence, density, and characteristics of inflammatory infiltrations in swine with Helicobacter sp., and particularly Candidatus Helicobacter suis (CHS) infections. Gastric samples from 29 pigs were obtained from slaughterhouses. Helicobacer genus-specific and CHS-specific primers sets were used to determine the Helicobacter status in all animals, and density and characteristics of inflammatory infiltration were estimated during microscope examinations. Helicobacter infections were observed in all the examined cases, and gastritis was noted in most cases. Mononuclear cells (lymphocytes and plasma cells) mostly dominated in the inflammatory infiltrations, and neutrophils were observed more seldom. It was noted that pigs which had Helicobacter infections in this part of the gastric mucosa, also had more intensive inflammatory infiltrations than animals without infection. No differences in the density of inflammatory infiltrations in the body and pyloric antrum of the stomach were noted.
19
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Staphylococcus pseudintermedius - nowy, ale dobrze znany patogen

81%
Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.
Życie Weterynaryjne
|
2013
|
tom 88
|
nr 08
20

Preliminary data on Helicobacter sp. and candidatus Helicobacter suis infection rate in porcine gastric mucosa

81%
Fabisiak M., Sapierzynski R., Kizerwetter-Swida M.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 4
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