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1

Microstructure and chemical composition of leaf cuticular waxes in two Salix species and their hybrid

100%
Szafranek B., Tomaszewski D., Pokrzywinska K., Golebiowski M.
Acta Biologica Cracoviensia. Series Botanica
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2008
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tom 50
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nr 2
49-54
Leaf epicuticular wax morphology and chemical composition of total cuticular waxes were studied in two Salix species (Salix alba and S.fragilis) and their hybrid (S. x rubens). A smooth wax layer with small, scattered wax structures covered the adaxial leaf surface in all three taxa, and a crustlike wax layer composed of terminally fused wax filaments was present on the abaxial surface. The leaf cuticular waxes, both epicuticular and intracuticular, were obtained by hot extraction in chloroform and then analyzed by gas chromatography and mass spectrometry. The principal components of the waxes were primary alcohols, fatty acids, aldehydes, n-alkanes and wax esters. The qualitative composition of the waxes was quite similar but there were quantitative differences between the taxa. The epicuticular crystalline waxes are composed of very-long-chain aldehyde polymers.
2

Ocena niektorych cech jakosci nasienia buhajkow w centralnych wychowalniach okregu lubelskiego

100%
Stenzel R., Jasinska E., Szafranek B.
Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica
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1996
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tom 14
27-30
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Chemometrics as a tool for plant - inscet ecological studies

100%
Szafranek J., Nawrot J., Szafranek B., Malinski E.
Journal of Plant Protection Research
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2001
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tom 41
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nr 4
The process of plant selection by insects is mediated by repellents and attractants. Several compounds may be involved in this interaction. Thus intraspecific variation of the compounds concentration play an important role in the herbivory. The best tool for the characteristic of this variation is chemometrics. The strategy of the analysis with the use of literature data on terpenes and sesquiterpenes variations in Pinus caribaea needles in relation to Atta laevigata herbivory is exemplified herein. Simple cluster analysis and principal components analysis were used for the data study. Two factors were found to be sufficient to describe total variation in more than 90%. Factor 1 is responsible for repellent properties. From factor loading, the relevant chemical compounds were identified.
4

Separations of epicuticular waxes from potato leaves and bean seeds by high performance liquid chromatography using light-scattering detector

100%
Szafranek B., Synak E., Szafranek J.
Herba Polonica
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2004
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tom 50
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nr 3-4
Most epicuticular waxes require an initial fractionation into lipid classes prior to the further analysis. Using a silica gel column and a light-scattering detector (LSD), we developed an HPLC method to analyse and fractionate epicuticular waxes from Solanum tuberosum leaves and Plmseolus vulgaris seeds. Applying HPLC-LSD with ternary gradient clearly revealed the major peaks in potato waxes, which consist of alkanes in the C25-Cl3 range, sesquiterpene hydrocarbons, wax esters (Cj(i-C54), aldehydes (C24-C32), 2-ketones (C25-C33), 2-alkanols (C23-C!0), fatty acids (Cl6-C32), 1-alkanols (C22-C33), sterols, diols and liydroxyacids. The bean seed waxes contain n-alkanes (C25-C33), squalene, wax esters (C37-C58), fatty acids (C22-C32), 1-alkanols (CH-C35), sterols and triterpene alcohols.
5

Chemometrics in ecological studies

100%
Szafranek B., Malinski E., Szafranek J.
Bulletin of the Polish Academy of Sciences. Biological Sciences
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2002
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tom 50
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nr 1
6

Wplyw frakcji woskow powierzchniowych lisci ziemniaka na zerowanie larw stonki ziemniaczanej

88%
Szafranek B., Waligora D., Synak E., Szafranek J., Nawrot D.
Progress in Plant Protection
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2004
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tom 44
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nr 2
1131-1134
7
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A correlation by chemometric methods of Solanum tuberosum epicuticular wax components and plant infestation with Leptinotarsa decemlineata [Say]

88%
Szafranek B., Synak E., Nawrot J., Malinski E., Szafranek J.
Journal of Plant Protection Research
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2002
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tom 42
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nr 3
8

Woski powierzchniowe ziarna pszenicy jako stymulatory procesu skladania jaj samic wolka zbozowego [Sitophilus granarius L.]

76%
Nawrot J., Polanska A., Szafranek J., Malinski E., Synak E., Szafranek B.
Progress in Plant Protection
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2000
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tom 40
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nr 2
547-557
9

Correlation between bean seed surface lipids and Acanthoscelides obtectus Say development

76%
Nietupski M., Szafranek B., Ciepielewska D., Synak E., Fornal L., Szafranek J.
Journal of Plant Protection Research
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2005
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tom 45
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nr 2
125-133
The influence of bean seed surface lipids on infestation of seeds by Acanthoscelides obtectus Say was investigated. The experiments were performed in dual-choice bioassays on three bean varieties: Blanka, Bor and Longina. The collected data for natural and solvent washed seeds concerned the number of ovipositions, embryo mortality, lack of seed-boring activity, dead larvae inside seeds and developed insects. The results clearly indicated that bean seed surface lipids are involved in all infestation stages, and could be used to distinguish resistant and non-resistant varieties of been. Chemical analyses revealed the following groups of surface lipids: wax esters, long chain primary alcohols, n-alkanes, sterols, fatty acids, squalene, aldehydes, monoacylglycerols, ketones and fatty acid esters. Quantitative composition of surface lipids was analysed using selected chemometric procedures to determine correlation with bioactivity. Cluster analysis of surface lipid composition enabled to distinguish resistant and non-resistant varieties. Fatty acids and monoacylglycerols were found to deter bean weevil infestation, while alkan-1-ols acted as attractants
10

Badanie wplywu woskow powierzchniowych ziemniaka Solanum tuberosum ze szczegolnym uwzglednieniem seskwiterpenow oraz wybranych olejkow eterycznych na zachowanie stonki ziemniaczanej Leptinotarsa decemlineata

64%
Szafranek J., Malinski E., Nawrot J., Szafranek B., Synak E., Dubis E., Golebiowski M., Winiecki Z.
Progress in Plant Protection
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1998
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tom 38
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nr 1
36-43
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