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It is important to manage water resources of the upper Yellow River basin for the new Silk Road economic belt. In recent decades, under the combined human activities and influence of climate, the hydrologic regime of the upper Yellow River basin shows remarkable variations that have caused many issues. So potential human indicated influence has been drawing increasing attention from hydrologists and local governments. The aim of this study is to determine the changes in the hydrological characteristic parameters and mean annual runoff series of the upper Yellow River basin. This paper took the representative Lanzhou Station in the upstream Yellow River as an example, used the TFPW-MK mutation test and rank sum test to analyze the location of the variation points of hydrological series. By contrasting two different analysis results between natural and measured river runoff series, the impacts of human activities on the long-term hydrological regime were investigated. The variation range of hydrological ecological indexes before and after variation were analyzed by the method of indicators of hydrologic alteration (IHA). The results show: a) the hydrological series of Lanzhou Station is a significant decreasing trend can be observed in the natural stream flow series in 1985 with a high degree of hydrological variability, while human activities play an important role; b) various ecological indicators have changed in different degrees that have caused the deterioration of ecological conditions around 1985; and c) continuous decreasing stream flow in the upper Yellow River basin will trigger serious shortages of fresh water in the future, which may challenge the sustainability and safety of water resource development in the river basin, and should be paid great attention before 2020. Variation analysis and diagnosis of eco-hydrological indexes in the upper reaches of the Yellow River can provide a basis for the development, utilization, and protection of water resources in this area.
Day length is an important environmental factor affecting the growth and development of maize (Zea mays), a short day (SD) plant grown in different latitudes. Leaf has been recognized as the light perceiving and signal producing organ. Under long day (LD) conditions, photoperiod- sensitive induction phase in maize begins at the fourth fully expanded leaf stage. However, the changes of maize leaf proteome in response to LD are largely unknown. To reveal maize proteome response to LD, proteins extracted from newly expanded fifth, sixth and seventh leaves from maize inbred line 496-10 (photoperiod sensitive) and Huangzao4 (HZ4, photoperiod insensitive) under LD treatments were compared via gel-based proteomic approach. As a result, eleven differentially expressed proteins were identified between 496-10 and HZ4 by mass spectrometry. This difference in protein accumulation was highly reproducible during the fifth to seventh leaf stages and most obvious at the seventh leaf stage. The identified proteins are mainly involved in circadian clock or iron metabolism, light harvesting and photosynthesis, nucleic acid metabolism and carbon fixation or energy metabolism. This study provides new insight into the influences of LD treatment on SD plants, such as maize, at proteome level.
The aim in this study was to assess relationships between men exposure to lead and semen-quality parameters in an environmental pollution site. We recruited 95 men through the pollution area and two controls. We measured semen quality using computer-aided semen quality analysis, and lead levels in seminal plasma using graphite gurnace atomic absorption spectroscopy. The results showed that the semen quality of men from the pollution area was lower than those of other control districts. The mean of seminal plasma lead value in the pollution area were higher than reference values for controls. Seminal plasma lead values displayed a significant negative correlation with norm morph sperm rates. Male reproductive health may be threatened by environmental pollution, and it may be influenced by local population diathesis.
Background: Anti-angiogenesis therapy that targets VEGF is one of the important treatment strategies in advanced ovarian cancer. However, depending on the pharmaceutical agent, treatment can have undesirable side effects. SEMA4D has recently gained interest for its role in promoting angiogenesis. Here, we try to further understand the mechanism by which SEMA4D promotes angiogenesis in ovarian cancer. Methods: Correlation and western blot assaya were used to detect the relationship between VEGF and SEMA4D in clinical tissues and cells. Vasculogenic mimicry and transwell migration analyses were used to detect the roles of VEGF, SEMA4D and plexin-B1 on vasculogenic mimicry and migration. Vascular density and SEMA4D expression was determined using immunofluorescence staining in clinical tissues of EOC. Western blot was used to detect the expressions of CD31, MMP2 and VE-cadherin. We also analyzed the relationship between VEGF-SEMA4D and malignant tumor prognosis. Results: We found that knockdown of VEGF could suppress SEMA4D expression and that the expressions of VEGF and SEMA4D have a positive correlation in EOC cancer tissues. Vasculogenic mimicry and transwell migration analyses showed that SEMA4D and VEGF have a synergistic effect on the promotion of angiogenesis in A2780 and HUVEC cells. Soluble SEMA4D (sSEMA4D) could promote VM and migration in A2780 and HUVEC cells via the SEMA4D/plexin-B1 pathway, but the effect was not noted in stably transfected shR-plexin-B1 cells. In clinical tissues of EOC, the vascular density and SEMA4D/plexin-B1 expression were higher. When VEGF, SEMA4D and plexin-B1 was knocked down, the expression of CD31, MMP2 and VE-cadherin, which are the markers and initiators of angiogenesis and the epithelial–mesenchymal transition (EMT) process were reduced. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. Conclusions: VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer. Targeting VEGF and the SEMA4D signaling pathway could be important for the therapy for EOC.
Satellite hardware has reached a level of development that enables imaging satellites to realize applications in the area of meteorology and environmental monitoring. As the requirements in terms of feasibility and the actual profit achieved by satellite applications increase, we need to comprehensively consider the actual status, constraints, unpredictable information, and complicated requirements. The management of this complex information and the allocation of satellite resources to realize image acquisition have become essential for enhancing the efficiency of satellite instrumentation. In view of this, we designed a satellite auto mission planning system, which includes two sub-systems: the imaging satellite itself and the ground base, and these systems would then collaborate to process complicated missions: the satellite mainly focuses on mission planning and functions according to actual parameters, whereas the ground base provides auxiliary information, management, and control. Based on the requirements analysis, we have devised the application scenarios, main module, and key techniques. Comparison of the simulation results of the system, confirmed the feasibility and optimization efficiency of the system framework, which also stimulates new thinking for the method of monitoring environment and design of mission planning systems
An electrolysis process method for free-form blade surface finishing is proposed for a free-form surface impeller, and a stepwise method is used to process the inter-blade channel of the overall impeller. The forming cathode is then used to finish the blade to meet the blade processing requirements. In the design, the forming cathode structure was improved by using motion simulation software, and the flow field simulation software was used to simulate and analyze the cathode flow channel. The cathode shape and the electrolyte flow rate between the electrodes meet the processing requirements. In the process of processing experiments, the motion path of the cathode was analyzed and optimized. The effect of the feed direction on the uneven distribution of the blade machining gap was reduced through optimization, and high-frequency pulse power processing was used to reduce the machining gap and improve the machining accuracy of the blade. The experimental results show that the process scheme is feasible and the precision of the processed impeller free-form surface is significantly improved. The material is a monolithic turbine disk of high-temperature alloys, and its large twisted blade processing has always been a problem in the manufacturing industry
Negative energy balance (NEB) is a common pathological cause of ketosis. As the major organs of lipid metabolism, the liver and fat tissue take part in regulating lipid oxidative capacity and energy demands, which is also a key metabolic pathway that regulates NEB development during the perinatal period. Fibroblast Growth Factor 21 (FGF21) is a novel metabolic regulator involved in the control of fatty acid oxidation and lipid metabolism during a prolonged negative energy balance. Our study determined a correlation between serum FGF21 and β-hydroxybutyric acid (BHBA) levels in dairy cows with ketosis. We used sixty cows with low milk yield, abnormal glucose metabolism, and ketosis. Serum FGF21 and BHBA levels were measured using commercial kits. Serum FGF21 increased with increasing BHBA levels up to 1.6 mmol/L. At BHBA levels > 1.6 mmol/L, FGF21 decreased. Serum FGF21 levels were positively associated with BHBA levels, particularly in dairy cows with subclinical ketosis (r = 0.647, P < 0.01). At BHBA levels between 1.2 mmol/L and 1.6 mmol/L, FGF21 was more closely correlated with BHBA than with other metabolic parameters. At BHBA levels > 1.6 mmol/L, the association between FGF21 and BHBA was not significant. In conclusion, our results show that FGF21 was closely related with SK in cows. FGF21 may be a promising regulator in the prevention of subclinical ketosis.
Greenhouse gas (GHG) emissions from municipal solid waste (MSW) management significantly contribute to high global warming potential (GWP). However, most studies have failed to facilitate identifying MSW management schemes capable of comprehensively meeting the goals from decision-makers at different hierarchical levels under uncertainties. This study develops an inexact bi-level linear programming (IBLP) model for collaborative control of GHG emissions and waste management in Beijing: MGU-MCL. The MGU-MCL model implies a leader-follower decision process, with the environmental sector providing the upper-level objective and the local authority dominating the lower-level objective. Then, an interactive fuzzy possiblistic approach is introduced to represent the satisfactory degrees of different decision-making levels. Results show that the MGU-MCL model decisions would reduce GHG emissions by about 9%, but increase management costs by 4% compared with the decisions from conventional models; the contribution of the landfill facilities to GHG emissions would be predominant, especially methane emissions; while the composting and incineration facilities would account for a large proportion of management cost. Further comparative analysis among the bi-level and single-level models indicates that the bi-level model could provide coordinated schemes under an integrated consideration of economic efficiency and environmental impact.
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Brain endocasts are rare in the fossil record because they are only preserved under exceptional conditions. An equid brain endocast from the early Pleistocene of Wanrong County, Shanxi Province, China, is reported in this paper. Measuring approximately 140 × 95.2 × 83 mm, the new specimen represents a relatively advanced adult horse brain. Comparisons indicate that it is more derived than those of Hyracotherium and Mesohippus in having an expanded neocortex, and more than those of Pliohippus and Hipparion in having an enlarged network of branching sulci; in most characters involving these sulci, the Shanxi brain conforms to the extant species Equus caballus. The sulcus diagonalis of the Equus brain appears to have evolved conservatively during the early Pleistocene, whereas the sulcus suprasylvius seems to have evolved rapidly. The specimen demonstrates that the development of a high degree of complexity predates the enlargement of the brain in the horse, which increased in length, breadth, and especially height during the late Cenozoic.
Drought and ultraviolet-B (UV-B) radiation are the most adverse factors for plant growth and productivity. The aim of this study was to compare the effects and interaction of drought and UV-B radiation in the seedlings of maize and soybean. The relative leaf water content was the main factor resulting in reduced growth in response to drought. The anthocyanin and phenol levels were increased under UV-B radiation. Drought and UV-B radiation caused great membrane damage, as assessed by lipid peroxidation and osmolyte leakage. Catalase, peroxidase, superoxide dismutase and hydrogen peroxide were observed under both stress conditions. Proline increased primarily in drought-stressed seedlings and may be the drought-induced factor with a protective role in response to UV-B. The total dry matter of both plant species was decreased due to the chlorophyll content and photosynthetic rate decreased under single and combined stress conditions. UV-B radiation had more adverse effects on growth of maize and soybean seedlings than drought by the physiological and biochemical parameters measured.
A dynamic habitat and population model has been developed and applied to the tailwater below Glen Canyon Dam to estimate the dam operation’s effects on rainbow trout (Oncorhynchus mykiss) habitat and population dynamics. Water depth, flow temperature, flow velocity, and substrate types were used as the suitability parameters and to evaluate these indicators. Historic hydraulic data from 1991 to 2009 were analyzed to determine the minimum, mean, and maximum flow rates that were used to represent the habitat suitability index (HSI) and overall suitability index (OSI) of the rainbow trout fry, adult, and spawning life stages, respectively. Fish abundance and the simulation results were also compared with observed fish numbers. Results indicated that under the historic dam operation, the habitat suitability level in the Colorado River was not suitable for fry and spawning rainbow trout, but very suitable for adult rainbow trout. It is indicated that high HSI levels do not mean high fish abundance. It can also be seen that overall rainbow trout abundance decreased during the period 1991 to 2009.
A modified method of injection into the cerebellomedullary (CM) cistern of mice was developed based on fixation of the mouse with a special mask under inhalation anesthesia, and exposure of the sagittal suture of the cranium and midline of the nape to allow us to visualize injection point directly. The accuracy of the modified method was evaluated using the temporal and spatial intracranial distribution of dye by intracisternal injection of methylene blue aqueous solution. A high concentration of dye was found in the CM cistern, the ventral cisterns, and intracranial proximal portion of the main cranial nerves at 1 hour after injection. The color of the dye in the CM cistern and the ventral cisterns was lighter, and the dye had reached the intracranial distal portion of the main cranial nerves at 6 hours after injection. The dye was completely eliminated by cerebrospinal fluid (CSF) circulation at 24 hours after injection. No severe brain injury was found in any of the 20 mice. Intracisternal injection was successful in all 14 mice sacrificed 1 hour or 6 hours after injection according to the confirmation of dye distribution. The effects of central administration of endothelin-1 (ET1) were evaluated on cerebral blood supply, constriction of cerebral arteries, and change of respiration in mice. Three doses of ET1 were studied: 2 ^g (0.8 nmol), 4 ^g (1.6 nmol), and 6 ^g (2.4 nmol). Cerebral blood flow (CBF) was monitored for 60 minutes following injection using a laser Doppler probe. Intracisternal ET1 injection induced dose-dependent reduction of CBF, constriction of cerebral arteries, and respiratory depression in mice. This modified method of injection into the CM cistern under direct visualization provides accurate and reproducible injection into the CSF, and can be used to investigate the effects of various chemical substances on the central nervous system in mice.
Silicon (Si) can enhance plant defense against biotic and abiotic stresses, but little is known of its possible alleviation of aluminum (Al) stress. In this study, we find out how Si may mediate Al stress based on changes in root morphological parameters, biomass, physiological attributes and concentrations of Al and Si in peanut (Arachis hypogaea L., cv. Zhongkaihua 99). The peanut was raised with (80 mg L⁻¹) or without Si in the growth chamber under 0 and toxic Al (160 mg L⁻¹) levels. Aluminum stress reduced the root dry weight by 52.4 %, shoot dry weight by 33.9 % and root-to-shoot ratio (R/S) by 28.8 %. However, it increased the activities of catalase in leaves and roots by as much as 161.6 and 149.0 %, superoxide dismutase by 141.7 and 147.0 %, and peroxidases by 62.0 and 64.1 %. The Si-treated peanut suffered less from Al stress through improvements in photosynthesis, biomass and R/S. The malondialdehyde, an index of membrane damage decreased significantly by 26.0 and 28.2 % in peanut leaf and root with silicon application under Al toxicity. For the peanut treated with Al, tissue concentration of Al increased by 371.5 % in the root, 20.9 % in the stem and 37.8 % in the leaf, much of the uptake was partitioned to the root. These concentrations decreased by 40.7, 5.3 and 25.6 %, respectively, following Si application.
The prolactin receptor (PRLR) is a receptor for a polypeptide hormone that stimulates mammary gland development and lactation in mammals. Associations between the PRLR genotype and cow and goat milk traits have been reported. To date, the nucleotide polymorphisms within the PRLR gene in equine species have not been investigated. Single-nucleotide polymorphism (SNP) is the most abundant form of genetic variation and a resource for useful genetic traits. In the present study, SNPs within the coding regions of the PRLR gene were detected by polymerase chain reaction-single-strand conformation polymorphism (SSCP) and DNA sequencing on the Yili horse of China. Ten novel SNPs were identified in the PRLR gene: one located within exon 4 (XM_001500104: c.156 C>T); one within exon 5 (c.312 T>C); and eight within exon 10 (c.1062 T>C, c.1083 G>A, c.1226 A>C, c.1233 C>T, c.1279 C>A, c.1749 A>T, c.1764 G>C, and c.1778 T>A). The mutation allele frequencies and effective allele numbers ranged from 0.10 to 0.30 and from 1.2195 to 1.7240, respectively. Four of the identified SNPs are non-synonymous and involve four amino acid substitutions, namely, p.H409P (c.1226 A>C), p.H427N (c.1279 C>A), p.Q588H (c.1764 G>C), and p.I593K (c.1778 T>A), in the cytoplasmic domain of the PRLR, which plays a fundamental role in signal transduction. Theoretically, the four nucleotide substitutions would result in altered spatial structure and functions of the PRLR molecules. Further study is required, however, to determine whether these mutations affect the milk yield of the Yili horse.
The aim of this study was to assess the effect of sodium alginate-immobilized bacteria and ultrasonic assistance on the biodegradation of oestrogens in soil. The studied oestrogens were oestrone (E1), oestradiol (E2), oestriol (E3), 17α-ethinylestradiol (EE2), and bisphenol-A (BPA). A central composite design was developed to determine the optimal conditions of the three variables (ultrasound time, sodium alginate concentration, and amount of sodium alginate beads) for the removal of oestrogens. Moreover, the experiment utilized a quantitative structure-biodegradation relationship (QSBR) to analyze the effect of the estrogenic physicochemical properties on the enhancement of the biological degradation mechanism. The results indicated that the optimal conditions are an ultrasound time of three min, a sodium alginate concentration of 3%, and 4 g of sodium alginate beads. These conditions resulted in removal rates of 100%, 100%, 93%, 96.47, and 51.87% for E1, E2, EE2, BPA, and E3, respectively, after seven days. These rates were 1.7, 1.4, 1.3, 1.2, and 2.1 times the microbial degradation rate of the suspended state, respectively. Based on a Pearson correlation analysis, the oestrogen molecule polar surface area (PSA) and hydrophobicity (represented by logKow) were significantly related to the effect of biodegradation. An analysis of the OSBR model (with the oestrogen biodegradation rates as a dependent variable and PSA and logKow as independent variables) indicated the following: PSA negatively correlated and logKow positively correlated with oestrogen removal, and these effects were synergistic. Therefore, sodium alginate/ultrasound assistance can significantly improve the biodegradation rates of oestrogens in soil, while simultaneously adjusting other environmental conditions would influence and control the biodegradation processes of oestrogens.
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