Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 28

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Zastosowanie biotechnologii w ochronie roślin przed nicieniami

100%
Obrepalska-Steplowska A.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 3
Plant parasitic nematodes feed and develop on many economically important crop species causing high losses amounting to many billions dollars every year throughout the world. This negative balance make them the second most damaging group of pathogen/pests after fungi. Additionally, some of them are viral vectors that is contributing to their higher extent of harm caused. Nevertheless, plant nematology is underrepresented in terms of staff in comparison to other fields focusing on plant pests and pathogens. Development in biotechnology however, caused also the development in plant nematology providing tools for characterization or identification of nematode species. New modern diagnostics approaches are continuously described on the basis of genome analysis. Also in Plant Protection Institute – National Research Institute several studies are conducted that concentrate on populations variability of economically important nematode species, diagnostics, and plant-nematode interactions. Another aspect of interest involving biotechnologists is characterization of host reaction to plant-parasitic nematode infection and description of genes participating in production of resistance against those pests. The use and generation of resistant cultivars is a powerful tool in reducing the spread of some quarantine nematodes. Since nematodes are very difficult to destroy, their reduction is a major challenge for plant protection. The use of biotechnology along with other alternative approaches may help to achieve this goal.
2

Rola satRNA w patogenezie szczepow wirusa karlowatosci orzecha ziemnego [PSV] wystepujacych w Polsce

100%
Obrepalska-Steplowska A.
Rozprawy Naukowe Instytutu Ochrony Roślin
|
2010
|
nr 22
1-110
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Analysis of the interaction between tomato torrado virus proteins using the yeast two-hybrid system

63%
Budziszewska M., Obrepalska-Steplowska A.
Journal of Plant Protection Research
|
2013
|
tom 53
|
nr 4
Ten years ago for the first time the new picorna-like virus species - Tomato torrado virus (ToTV) - was found and described on tomato plants. The isolates of this pathogen were reported in Europe, America, and Oceania including Australia. Because of its unique biological and molecular features, ToTV was classified to the new genus Torradovirus, in the Secoviridae family. In Poland, three isolates: Wal’03, Kra, and Ros ToTV were identified on greenhouse tomato cultivars. At present, the biology and the genome structure of this virus are characterised. But there is no data extending beyond the bioinformatics analyses about the function of viral proteins, polyproteins, and non-coding sequences, as well as possible interactions between viral, host and vector factors that may be important for the infection process, encapsidation, transport in plants, and transmission. In this study, we have undertaken a search for the possible protein-protein interaction of ToTV encoded proteins using the yeast two-hybrid (Y2H) system. The viral genome fragments covering full sequences for nine known proteins of ToTV were amplified using specific primers with characteristic recombination sites. This process enabled the construction of basic entry clones for each protein that further facilitated manipulations with prepared constructs using Gateway technology. Two-hybrid assays were performed in the yeast strain and tested interactions of ToTV proteins were analysed in several combinations using auxotrophy markers. Our analyses did not reveal the presence of interactions between ToTV domains. Surprisingly, no interactions were found in the case of various CP subunits as well as between CP subunits and 3A protein, that in some virus families are known to play a role in viral life cycle. This role includes virion assembly or cell-to-cell transport. The lack of interactions may be a result of the limitation of this experimental system, or suggest that these proteins may interact indirectly, or require the presence of genomic RNAs or some host factors.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Multiplex RT-PCR Reaction for Simultaneous Detection of Tomato Torrado Virus and Pepino Mosaic Virus Co-Infecting Solanum Lycopersicum

63%
Wieczorek P., Obrepalska-Steplowska A.
Journal of Plant Protection Research
|
2013
|
tom 53
|
nr 3
The tomato (Solanum lycopersicum L.) is cultivated all over the world and is a vegetable of significant economic importance. However, an increased production of the vegetable is directly connected with an elevated occurrence of pathogens limiting the production efficiency of the vegetable. Both, Tomato torrado virus and Pepino mosaic virus have been found to be serious disease factors. When not controlled, these viruses can significantly decrease tomato cultivation. In this article, we report a multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocol for simultaneous detection of both, Tomato torrado virus (ToTV) and Pepino mosaic virus (PepMV) in virus infected plants. The assay was designed to specifically amplify the conserved regions of genomic ribonucleic acid (RNA) of both viruses. Moreover, the glycerandehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control of amplification to exclude false-negative assay results. High-resolution melt analysis of generated RT-PCR products was additionally performed to increase sensitivity and double-check the specificity of the reaction without the need of subsequent complementary deoxyribonucleic acid (cDNA) sequencing.
5

Wybrane mechanizmy nabywania odpornosci organizmow na srodki ochrony roslin

63%
Nowaczyk K., Obrepalska-Steplowska A.
Postępy Biologii Komórki
|
2006
|
tom 33
|
nr 1
137-158
6

Czynniki chorobotworcze w biologicznym zwalczaniu nicieni-szkodnikow roslin

63%
Obrepalska-Steplowska A., Sosnowska D.
Biotechnologia
|
2008
|
nr 2
115-130
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

51%
Przybylska A., Fiedler Z., Obrepalska-Steplowska A.
Journal of Plant Protection Research
|
2016
|
tom 56
|
nr 1
Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.
8

Diagnostyka molekularna polskich populacji korzeniaka pospolitego (Pratylenchus neglectus)

51%
Kierzek D., Dobosz R., Jeszke A., Obrepalska-Steplowska A.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 4
Root-lesion nematodes, Pratylenchus spp., are economically important, plant-damaging parasitic nematodes occurring in a wide variety of crops. The most common species is Pratylenchus neglectus which is found on cereal crops, especially on wheat. The proper identification of this nematode species is crucial because of significant morphological similarity to another Pratylenchus species. There are some diagnostic methods described in publication. The aim of this work was to test previously described protocol and its usefulness for Polish populations of this pathogen. Moreover, the new specific PCR protocols were described. These methods can be used both, for identification of Polish population of P. neglectus, and on the basis of the amplified rDNA region for the genetic correlation and phylogenetic studies.
9

Analysis of P1 regulatory region of IGF-1 gene in children with growth retardation

51%
Obrepalska-Steplowska A., Kedzia A., Pacholska J., Gozdzicka-Jozefiak A.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
10

Characterization of the rDNA sequence of rose thrips (Thrips major Uzel) and its phylogenetic analysis

51%
Jeszke A., Fiedler Z., Obrepalska-Steplowska A.
Progress in Plant Protection
|
2013
|
tom 53
|
nr 4
Thrips major is a polyphagous insect, which occurs on each continent. In Poland T. major usually attacks crops, ornamental plants and herbs. Although it is very common pest, there are only few fragments of its sequence available. Knowledge about its rDNA (ribosomal deoxyribonucleic acid) sequence is needed to develop new diagnostic methods that could allow for successful differentiation between T. major and other thrips species such as Frankliniella occidentalis and Thrips tabaci, which post higher risk to cultivations in Poland. The aim of this work was the characterization of rDNA sequence of T. major, its comparison with other thrips species’ sequences followed by phylogenetic analysis. In this work new PCR (polymerase chain reaction) primers were designed which enabled rDNA fragment amplification from most of Thripinae subfamily species. PCR reaction was carried out and obtained products of amplification were sequenced, followed by bioinformatic analysis. Then, T. major 18S-ITS1-5,8S-ITS2-28S rDNA region sequences were compared with already known sequences of following thrip species: T. tabaci, T. palmi, Scirtothrips dorsalis, Echinothrips americanus, Frankliniella schulzei, F. occidentalis and F. intonsa and phylogenetic analyses were performed.
11

Wstępne badania nad zdolnością czynników kodowanych przez wirusa nekrozy pomidora (ToTV) do supresji PTGS

51%
Wieczorek P., Budziszewska M., Obrepalska-Steplowska A.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 3
Postranscriptional gene silencing, PTGS, is one of the important plant defense mechanisms induced during viral infection. The process is sequence-specific and results from the occurrence of dsRNA intermediate molecules generated during replication of viruses in the cytoplasm. The presence of dsRNA molecules activates the cascade of reactions in the host’s cells, leading to elimination of virus’ genetic material. In spite of its high efficiency, the PTGS can be inhibited by virus-encoded factors, usually proteins, that enable virus replication progress and spread of the pathogen in the host. In the study we used an experimental model in which stably expressed green fluorescent protein in Nicotiana benthamiana 16c plants, was locally silenced with hairpin RNA construct, whereas virus-encoded PTGS putative suppressors were delivered by infecting the plants with ToTV. We have shown that Tomato Torrado Virus-encoded factors can efficiently suppress PTGS induced in used tobacco plants infected with the ToTV. In contrast, in the leaves of N. benthamiana with induced PTGS we identified lower expression rate of the GFP when not infected with the virus. This observation indicates that ToTV encodes strong PTGS suppressor (or suppressors). Nature and mechanism of which will need to be determined in further research.
12

Subwirusowe czasteczki RNA zwiazane z wirusami roslinnymi typu ss[plus]RNA

51%
Pelczyk M., Obrepalska-Steplowska A., Pospieszny H.
Postępy Biochemii
|
2006
|
tom 52
|
nr 2
212-221
13

Complete nucleotide sequence of a Polish strain of peanut stunt virus [PSV-P] that is related to but not a typical member of subgroup I

51%
Obrepalska-Steplowska A., Budziszewska M., Pospieszny H.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 4
731-739
Peanut stunt virus (PSV) is a common legume pathogen present worldwide. It is also infectious for many other plants including peanut and some vegetables. Viruses of this species are classified at present into three subgroups based on their serology and nucleotide homology. Some of them may also carry an additional subviral element — satellite RNA. Analysis of the full genome sequence of a Polish strain — PSV-P — associated with satRNA was performed and showed that it may be classified as a derivative of the subgroup I sharing 83.9–87.9% nucleotide homology with other members of this subgroup. A comparative study of sequenced PSV strains indicates that PSV-P shows the highest identity level with PSV-ER or PSV-J depending on the region used for analysis. Phylogenetic analyses, on the other hand, have revealed that PSV-P is related to representatives of the subgroup I to the same degree, with the exception of the coat protein coding sequence where PSV-P is clustered together with PSV-ER.
14

A characteristic of satRNAs associated with Polish strains of Peanut Stunt Virus

51%
Obrepalska-Steplowska A., Pelczyk M., Pospieszny H.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
15

Analysis of expression of GAPDH gene in Solanum lycopersicum L. infected with Tomato torrado virus (ToTV)

51%
Wieczorek P., Budziszewska M., Obrepalska-Steplowska A.
Progress in Plant Protection
|
2013
|
tom 53
|
nr 2
Real-time PCR (polymerase chain reaction) is a method commonly used for analysis of genes expression. However, accurate interpretation of real-time PCR results needs additional normalization step, where the expression level of analyzed gene is corrected to the actual amount of total RNA taken for the analysis. As a normalizer any gene can be used, provided its expression is stable during the experiment. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is one of candidates that might be used as a reference gene for normalization of real-time PCR. Here we indicated a high identity of GAPDH mRNA sequence isolated from ten varieties of tomato (Solanum lycopersicum). Moreover, we reported here, that expression of the gene was rather unstable in tomato during Tomato torrado virus infection (ToTV).
16

Comparative analysis of 2a polymerase protein sequence of chosen Polish strains of Peanut stunt virus, PSV

51%
Budziszewska M.-A., Pospieszny H., Obrepalska-Steplowska A.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
17

The analysis of the rDNA sequence in populations of western flower thrips (Frankliniella occidentalis Pergande) from Wielkopolska

51%
Jeszke A., Fiedler Z., Obrepalska-Steplowska A.
Progress in Plant Protection
|
2012
|
tom 52
|
nr 3
Western flower thrips (Frankliniella occidentalis Pergande) is an economically important pest in greenhouse crops. Successful control of this pest is very difficult. Larvae of this pest are vectors of Tomato spotted wilt virus (TSWV) and Tobacco streak virus (TSV). The aim of this work was to analyze ribosomal DNA sequences of western flower thrips populations originating from different regions of the Wielkopolska province and to make phylogenetic studies based on those sequences. In this work new Polymerase Chain Reaction (PCR) primers were used which enable rDNA fragment amplification. PCR reaction was carried out and its products were sequenced, followed by bioinformatic analysis. The identity level of analyzed populations from Wielkopolska was 98.2–100% and identity between analyzed populations and reference population from the GeneBank database was about 98.7–99.7%. Phylogenetic studies were also made. The results of this work will be useful to develop new diagnostic methods to distinguish western flower thrips and other thrips species presenting a threat in Poland.
18

Poziom odpornosci polskich populacji slodyszka rzepakowego [Meligethes aeneus F.] na pyretroidy: mechanizmy odpornosci w swietle badan molekularnych

51%
Wegorek P., Obrepalska-Steplowska A., Nowaczyk K., Zamojska J.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 1
383-388
19

Analysis of mtCOII genes of nematodes from genus Globodera and their utility for detection and differentiation

51%
Nowaczyk K., Obrepalska-Steplowska A., Kornobis S., Dobosz R.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
20

Globodera artemisiae [Eroshenko et Kazachenko, 1972] [Nematoda: Heteroderidae] from Poland

51%
Dobosz R., Obrepalska-Steplowska A., Kornobis S.
Journal of Plant Protection Research
|
2006
|
tom 46
|
nr 4
403-407
Globodera artemisiae (Eroshenko et Kazachenko, 1972) was found in Poland in autumn of 2004. The nematodes developed on Artemisia vulgaris L. Morphological and morphometric characteristics of the Polish population correspond to earlier known populations from Far East of Russia, Armenia, China, Germany and Sweden. The traditional identification was confirmed by molecular methods. On the basic of rDNA sequences of G. artemisiae, deposited in GenBank the product of expected size was obtained. Subsequently, the results were confirmed by sequencing analysis.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.