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1

Effect of different workload and hydrocortisone in vitro on platelet aggregation in athletic horse

100%
Casella S., Giannetto C., Giudice E., Piccione G.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 3
2

Circadian activity rhythm in sheep and goats housed in stable conditions

100%
Piccione G., Giannetto C., Casella S., Caola G.
Folia Biologica
|
2008
|
tom 56
|
nr 3-4
133-137
3

Serum homocysteine and oxidative stress evaluation during exercise in horse

88%
Fazio F., Casella S., Giannetto C., Caola G., Piccione G.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 2
169-174
The aim of the present work was to evaluate the influence of exercise on serum homocysteine and oxidative stress in Thoroughbreds during official races. For our study, ten Thoroughbred mares, 5 years old and clinically healthy, were used. The horses were trained to take part in an official 2100 meter race. Reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. Blood samples, collected from each horse at rest, immediately after the race, and 30 and 180 min after the end of the race, were collected by jugular venipuncture using vacutainer tubes with no additive for the assessment of dROMs, Oxy-adsorbent and SHp by means of a UV spectrophotometer, while serum total Hey values were determined by high performance liquid chromatography with fluorimetric detection and isocratic elution. Statistical analysis, one way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P<0.05 was considered statistically significant) for all parameters studied. Moreover, a coefficient of linear correlation (r) was computed for values of dROMs and sHcy for all sampling times but a significant linear regression (r = 0.94) was found only after the race. The variations of sHcy, dROMs, Oxy-adsorbent and SHp during the experimental period considered suggest the important role of oxidative status in the athletic horse. The systematic analysis of oxidative stress and its influence on homocysteine levels contribute to the clinical evaluation and assessment of the athletic performance of the horse.
4

Nictemeral profile of platelet aggregation and clotting parameters in horses during training

88%
Casella S., Giannetto C., Fazio F., Assenza A., Piccione G.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 4
The daily rhythm of platelet aggregation and clotting parameters during training were investigated in six Thoroughbred horses. Blood samples were collected every 4 h for 48 h. Platelet aggregation was measured with an aggregometer and subsequently according to the trends recorded, the percentage of aggregation was calculated. Prothrombin time (PT), activated partial thromboplastin time, thrombin time, and fibrinogen concentration were assessed by means of a coagulometer. A one-way repeated- measures analysis of variance was used to determine significant differences (P<0.05) and the single cosinor method was applied. On each single day, a significant effect of time on the percentage of aggregation and PT was observed. A cosinor analysis identified the nictemeral rhythm of percentage of aggregation and PT that showed a nocturnal acrophases on both days 1 and 2. We can assert that physical exercise could be an event regulating the daily rhythms of platelet aggregation and clotting parameters in the horse.
5
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Leukocyte modifications during the first month after foaling in mares and their newborn foals

76%
Piccione G., Rizzo M., Arfuso F., Giannetto C., Di Pietro S., Bazzano M., Quartuccio M.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 3
During early post-partum period both neonatal foals and peripartum mares are most susceptible to diseases. The aim of this study was to establish physiologic modifications of leukogram during the first month after foaling in mares and their newborn foals. To this end blood samples were collected from nine mares and nine foals (T0-T10), every three days from the 1st day until the 30th day after foaling. Samples were analysed for white blood cell (WBC) count and differential leucocyte counts. Two-way repeated measure analysis of variance (ANOVA) showed, in postpartum mares WBC showed significant higher values at T0 (9.02±0.76) in respect to other time points, and at T2 (8.08±0.53) and T3 (7.92±0.59) compared to T1 (6.98±0.43), whereas in foals lower WBC values at T0 (6.11±0.49) compared to other experimental periods except T1 (6.90±0.94), and at T1 compared to T8 (7.95±0.61) and T10 (7.90±0.36) were observed. The differential leucocyte counts showed significant modifications in the percentage of neutrophils (π<0.001) and lymphocytes (p<0.001) both in postpartum mares and in foals during the experimental period. Furthermore ANOVA showed significant differences between postpartum mares and foals (P<0.01) in all studied parameters, and between postpartum mares and control mares in WBC and neutrophils values. The obtained results provide suitable information about the influence of foaling on leukogram of periparturient mares and reveal WBC dynamics in newborn foals during the first month post-partum.
6
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Different training schedules influence platelet aggregation in show jumping horses

76%
Giannetto C., Arfuso F., Fazio F., Giudice E., Di Pietro S., Bruschetta D., Piccione G.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
Depending on the intensity, duration and type of physical exercise, equine metabolism has to adapt to nervous, cardiovascular, endocrine and respiratory system requirements. In horses, exercise and training are known to have considerable effects on the mechanisms of hemostatic system involving platelet activity. The aim of the present study was to evaluate the effect of different training schedules on platelet aggregation in 15 Italian Saddle jumping horses. Animals were divided into three equal groups: Group A was subjected to a high intensity-training program; group B to a light training program, group C included sedentary horses. From each animal, blood samples were collected by jugular venipuncture at rest on the 1st, 3rd and 5th days, and afterwards, once a week, for a total of 5 weeks data recording, in order to assess the maximum degree of platelet aggregation and the initial velocity of aggregation (slope) platelet aggregation. Two-way analysis of variance (ANOVA) showed a significant effect of the different training schedules on studied parameters. The results revealed a different degree of platelet aggregation and a different initial velocity of platelet aggregation that changes during the different training schedules in horses that could represent a different protective endothelial mechanism. These findings could have an important role for a clearer knowledge of the physiological reference values of platelet aggregation and for a better interpretation of these variations during the training.
7

Selected biochemical serum parameters in ewes during pregnancy, post-parturition, lactation and dry period

76%
Piccione G., Caola G., Giannetto C., Grasso F., Calanni Runzo S., Zumbo A., Pennisi P.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 4
Assessed were changes in selected biochemical parameters taking place during different stages of pregnancy, post-partum, lactation and dry period in ewes. The observations were conducted on 10 clinically healthy Comisana ewes kept on one farm. Blood samples were withdrawn once before the morning feeding during dioestrus phase before pregnancy, and then on week 4, 11 and 18 of pregnancy, week 2 post-partum, week 5, 15 and 25 of lactation and finally once in a dry period (week 32). Blood serum total protein as well as serum albumin increased significantly during pregnancy compared to dioestrus phase. Compared to dioestrus, a significant increase during late gestation and a significant decrease during dry period was shown in blood urea. Total blood lipids showed a significant increase during pregnancy, post-partum and early lactation compared to dioestrus, while total cholesterol and triglycerides showed the opposite trend. The study showed marked changes in certain biochemical parameters of sheep blood serum during late pregnancy, post-partum, lactation and dry period.
8

Adrenocorticotrophic hormone and cortisol levels during late pregnancy and post-foaling period in mares

76%
Piccione G., Arfuso F., Abbate F., Giannetto C., Panzera M., Rizzo M., Fazio F.
Animal Science Papers and Reports
|
2017
|
tom 35
|
nr 2
The aim of this study was to evaluate the trend of adrenocorticotrophic hormone (ACTH) and cortisol levels in mares during peripartum period. Twelve pregnant mares (Group A) were weekly monitored from the last 6 weeks before foaling (6BF-1BF) until the first 3 weeks after foaling (1AF-3AF). Twelve non-pregnant non-lactating mares constituted the control (Group B). Jugular blood samples were analyzed for plasma ACTH and serum cortisol concentration. ACTH showed higher values (P<0.05) at 1BF compared to the postpartum data points (1AF, 2AF and 3AF) in Group A. Cortisol levels were higher (P<0.05) at 1BF and 2BF compared to the 3AF in Group A. A significant positive correlation between ACTH and cortisol values was found in mares from Group A throughout the peripartum period (Pearson’s r=0.40; P=0.0028). The Dunnet’s test showed lower ACTH values in Group A at postpartum data points than control, and higher cortisol levels in Group A throughout prepartum times and at 1AF than control (P<0.0001). The decrease of ACTH and cortisol levels found during the early postpartum period could indicate a reduced HPA response to physical and/or psychological stress during this physiological phase. This could help the mare to protect against stress-associated inhibition of lactation, relieve psychological stress, and enhance her immune function. Further studies involving the evaluation of prolactin and sex steroid hormones values are needed to fully understand the dynamic hormonal changes occurring in pregnant and lactating mares in order to permit clinicians to make appropriate interpretation of the results.
9

Influence of omega-3 in standardbred horse: haematological parameters

64%
Monteverde V., Congiu F., Vazzana I., Dara S., Guttadauro S., Di Pietro S., Giannetto C., Piccione G.
Annals of Animal Science
|
2016
|
tom 16
|
nr 1
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