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Studies were carried out on 32 one-day-old broiler chickens, which were divided into a control and 3 experimental groups. Chickens in the control groups were fed, for 8 weeks with basic feeds, while chickens in the experimental groups were fed basic feeds enriched with monensin-natrium at the following doses: group I — 100 ppm, group II — 70 ppm and group III — 50 ppm. During the course and at the end of the experiment liver, kidneys, pancreas and heart were removed from both the control and experimental chickens for microscopic examination. First structural changes in the form of a small degree fat — droplet formation in liver cells were observed after 4 weeks of the experiment. 8 weeks after the beginning of the experiment, a significant degree of fat — droplet formation and swelling of Golgi apparatus structure were noted. The changes observed were associated with side — effect of coccidiostatic action on the liver of the experimental broiler chickens.
In this study we investigated effects of bcl-2 antisense oligonucleotides (AS) and BAY K 8644 (B), calcium channel agonist, on human leukaemic cells from non-treatment patients with acute myeloblastic leukaemias. Apoptotic cells were observed in electron and fluorescent microscopes. When we examined the effects of AS + B on cells, level of bcl-2 mRNA decreased more as in cells stimulated by AS only. These observations indicate that AS with B in human leukaemic cells disturb cell viability by inducing apoptosis.
The paper presents the results of morphological examinations of the human cotyledons perfused in vitro and exposed to variable magnetic field (MF) during the 180-minute experiment. The cotyledon biopsies were collected immediately after perfusion and morphologically examined using the electron microscope. The control group C (10 perfusions) was not exposed to MF. In the experimental group E, (10 perfusions), the cotyledons were exposed to the 2mT, 50Hz variable magnetic field while in the experimental group E2 (10 perfusions) the 5mT, 50 Hz was used. In the groups E, and E2, numerous indentations of the sheath (areola) in the syntrophoblast nuclei were found, condensed (thickened) nuclear chromatin right beneath the sheath was substantially reduced and pyknosis was observed in some nuclei. The villi revealed widened vascular - epithelial membrane resulting from the oedema of the endothelial cells. Moreover, an increased number of collagen fibres in the villi and decreased number of active mitochondria were observed in the group E2
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