Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 6

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The Biolasol® liquid is an innovative solution used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity. Substances in the liquid prevent cellular oedema and help to maintain a proper water/mineral as well as acid/base balance in the intracellular environment. They also minimize free-radical injuries and ensure the integrity of the cellular membrane structure. The Biolasol® liquid has been shown to be much more efficient than the HTK liquid in the preservation of kidneys. The Biolasol® liquid containing 0.5 mM of vitamin C has been modified by adding ions of Se(IV), Zn(II), and their effect on the stability of the solution was examined. An accelerated aging test was applied to test the liquid stability. The test, based on the laws of chemical kinetics, was conducted at four temperatures at a 10°C step, that is: 50°C±0.05, 60°C±0.05, 70°C±0.05 and 80°C±0.05. The relative humidity equalled 75% of RH and the duartion of the test was 40 days. In order to determine the stability of the tested solutions, the Arrhenius Dependence equation was used, applied to the effect of temperature on the glucose decomposition reaction rate: lnk=lnA-(Ea/RT). The results indicate that the addition of zinc decreases the stability of the liquid by 30.5%, while the addition of selenium prolongs the stability by 8.21%. This is explained by the synergism of action of vitamin C and Se4+ antioxidant in the tested liquid. Zinc ions present in the solution increase the glucose decomposition reaction rate.
Arterial ketone index (AKBR) which is the ratio of acetoacetic acid to 3-hydroxybutyric acid in the arterial blood, is believed to reflect the mitochondrial reduction potential of hepatocytes and general energy state of the liver. In the presented paper we challenged this hypothesis by analysing the correlation between AKBR and the results of typical liver blood tests (AspAT, AlAT, LDH, CRP) and biotransforming potential of the liver (cytochromes P450, b5 and their corresponding NADPH and NADH reductases) in the model of ischemia-reperfusion injury of rat liver. The results were compared with histochemical analysis of distribution and activity of SDH, LDH and G-6-Pase, the key marker enzymes of the liver. We have shown that, except in the case of acute phase protein (CRP), a decrease in AKBR correlated well with the increase of the level of indicator enzymes in serum. Histochemical analysis also confirmed that AKBR correlates with the degree of damage to hepatocytes during early stage of reperfusion after 60 min of liver ischemia. In the Spearman test, AKBR was significantly correlated with the changes in cytochrome P450 content and its NADPH reductase activity which indicates a high sensitivity of this test. We conclude that the decrease of AKBR value reflects the impairment of basic energy pathways and detoxicative capability of the liver.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.