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Responses in growth, physiology and nitrogen nutrition of dragon spruce (Picea asperata) seedlings of different ages to enhanced ultraviolet-B

100%

Yao X., Liu Q.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 3

In the southeast of the Qinghai–Tibetan Plateau of China, dragon spruce (Picea asperata) is a key species and widely used in reforestation processes in the area. The paper mainly studied the effects of ultraviolet-B (UV-B) on growth, physiology and nitrogen nutrition of 3- and 6-year-old dragon spruce seedlings. The experimental design included ambient UV-B (control) and enhanced UV-B (+UV-B, a 30% increase). Enhanced UV-B significantly decreased growth, needle and root nitrogen concentration, needle nitrate reductase activity and increased UV-B absorbing compounds and malondialdehyde (MDA) content of two old dragon spruce seedlings. Glutamine synthetase activity was not affected by enhanced UV-B in two old dragon spruce seedlings. On the other hand, different old seedlings also exhibited different physiological responses to enhanced UV-B radiation. Chlorophyll content, carotenoids content and soluble protein content in 3-year-old seedlings significantly reduced by enhanced UV-B, but those in 6-year-old seedlings were not affected by enhanced UV-B. Proline content of 6-year-old seedlings were increased by enhanced UV-B. Compared with the 3-year-old seedlings, the 6-year-old seedlings showed lower reduction of growth and MDA content, and accumulated more proline and UV-B absorbing compounds for protecting seedlings under enhanced UV-B. The results implicated that 3-year-old seedlings were more sensitivity to enhanced UV-B than 6-year-old seedlings.

2

Photosynthetic and physiological responses of Swida hemsleyi [C.K. Schneid. et Wangerin] Sojak [Cornaceae] subjected to enhanced UV-B radiation and to nitrogen supply

100%

Yao X., Liu Q.

Polish Journal of Ecology

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2009

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tom 57

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nr 3

483-494

Atmospheric ozone remains depleted which in turn leads to the increase of UV-B radiation reaching the surface of the earth and in the same time more and more nitrogen will be imported into the terrestrial ecosystems through nitrogen deposition. These two factors will operate simultaneously. The photosynthetic and physiological responses of deciduous broad leaved species Swida hemsleyi occurring commonly at 1350–3700 m a.s.l. subjected to enhanced UV-B and to nitrogen supply were studied. The experimental design included two levels of UV-B treatments (ambient UV-B, 11.02 KJ m⁻² day⁻¹ and enhanced UV-B, 14.33 KJ m⁻² day⁻¹) and two nitrogen levels (without supplemental nitrogen supply and with supplemental nitrogen supply). An experiment was conducted in open semi-field condition in Maoxian Ecological Station of Chinese Academy of Sciences, Sichuan province, China at 1820 m a.s.l. Enhanced UV-B caused a marked decline in growth parameters, net photosynthetic rate, stomatal conductance to water vapour, chlorophyll pigments, whereas it induced an increase in rate of reactive oxygen species (ROS) production and ROS accumulation and malondialdehyde (MDA) content. Enhanced UV-B also induced an increase in leaf thickness and antioxidant compounds content, such as carotenoids and proline content. On the other hand, nitrogen supply caused an increase in some growth parameters, chlorophyll pigments and antioxidant compounds, and reduced ROS accumulation. However, nitrogen supply did not affect MDA content under enhanced UV-B, though it increased antioxidant compounds content and reduced the rate of ROS production and ROS accumulation. These results implied that enhanced UV-B brought harmful effects on Swida hemsleyi seedlings and supplemental nitrogen supply could alleviate the adverse effects of UV-B radiation on plants to some extent.

3

The effects of enhanced ultraviolet-B and nitrogen supply on growth, photosynthesis and nutrient status of Abies faxoniana seedlings

100%

Yao X., Liu Q.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 3

Abies faxoniana is a key species in reforestation processes in the southeast of the Qinghai-Tibetan Plateau of China. The changes in growth, photosynthesis and nutrient status of A. faxoniana seedlings exposed to enhanced ultraviolet-B (UV-B), nitrogen supply and their combination were investigated. The experimental design included two levels of UV-B treatments (ambient UV-B, 11.02 KJ m⁻² day⁻¹; enhanced UV-B, 14.33 KJ m⁻² day⁻¹) and two nitrogen levels (0; 20 g N m⁻²). The results indicated that: (1) enhanced UV-B significantly caused a marked decline in growth parameters, net photosynthetic rate (Pn), photosynthetic pigments and Fv/Fm, (2) supplemental nitrogen supply increased the accumulation of total biomass, Pn, photosynthetic pigments and Fv/Fm under ambient UV-B, whereas supplemental nitrogen supply reduced Pn, and not affect biomass under enhanced UV-B, (3) enhanced UV-B or nitrogen supply changed the concentration of nutrient elements of various organs.

4

Qualities and antioxidant activities of lotus leaf affected by different drying methods

61%

Guo C., Zhang N., Liu C., Xue Y., Chu J., Yao X.

Acta Physiologiae Plantarum

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2020

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tom 42

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nr 02

5

Differential expression of interferon-gamma, IL-4 and IL-10 in peripheral blood mononuclear cells during early pregnancy of the bovine

61%

Yang L., Wang Y., Li S., Zhu M., He K., Yao X., Zhang L.

Reproductive Biology

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2018

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tom 18

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nr 3

6

Brassinosteroids protect photosynthesis and antioxidant system of eggplant seedlings from high-temperature stress

61%

Wu X., Yao X., Chen J., Zhu Z., Zhang H., Zha D.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 02

The effects of 24-epibrassinolide under high temperature in eggplant (Solanum melongena L.) seedlings were studied by investigating the plant growth, chlorophyll content, photosynthesis and antioxidant systems. High temperature significantly inhibited the plant growth and markedly decreased the chlorophyll content, net photosynthetic rate, stomatal conductance and transpiration rate, while it increased intercellular CO₂ concentration. In a similar manner, high temperature also decreased significantly maximum quantum efficiency of PSII, potential photochemical efficiency, the quantum efficiency of PSII, photochemical quenching, the excitation capture efficiency of open centers, and increased non-photochemical quenching. Application of 0.05–0.2 μM EBR remarkably promoted the plant growth and alleviated high-temperature-induced inhibition of photosynthesis. Under high temperature, reactive oxygen species levels and lipid peroxidation were markedly increased, which were remarkably inhibited by application of 0.05–0.2 μM EBR. The activities of antioxidative enzymes such as superoxide dismutase, peroxidase, catalase and ascorbate peroxidase, and contents of ascorbic acid and reduced glutathione were significantly increased during high-temperature treatments, and these increases were more pronounced than those of EBR at 0.05–0.2 μM treatment. The EBR treatment also greatly enhanced contents of proline, soluble sugar and protein under high-temperature stress. Taken together, it can be concluded that 0.05–0.2 μM EBR could alleviate the detrimental effects of high temperatures on plant growth by increasing photosynthetic efficiency and enhancing antioxidant enzyme systems. Addition of 0.1 μM EBR had the best ameliorative effect against high temperature, while the addition of 0.4 μM EBR had no significant effects.

7

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Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein

51%

Wu X., Xiao L., Peng B., Wang Y., Yang Z., Yao X., Hu L., Lin X.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 1

African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.

8

Agonist-induced activation of human FFA1 receptor signals to extracellular signalregulated kinase 1 and 2 through Gq- and Gi-coupled signaling cascades

51%

Qian J., Gu Y., Wu C., Yu F., Chen Y., Zhu J., Yao X., Bei C., Zhu Q.

Cellular and Molecular Biology Letters

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2017

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tom 22

Ograniczanie wyników

Responses in growth, physiology and nitrogen nutrition of dragon spruce (Picea asperata) seedlings of different ages to enhanced ultraviolet-B

Photosynthetic and physiological responses of Swida hemsleyi [C.K. Schneid. et Wangerin] Sojak [Cornaceae] subjected to enhanced UV-B radiation and to nitrogen supply

The effects of enhanced ultraviolet-B and nitrogen supply on growth, photosynthesis and nutrient status of Abies faxoniana seedlings

Qualities and antioxidant activities of lotus leaf affected by different drying methods

Differential expression of interferon-gamma, IL-4 and IL-10 in peripheral blood mononuclear cells during early pregnancy of the bovine

Brassinosteroids protect photosynthesis and antioxidant system of eggplant seedlings from high-temperature stress

Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein

Agonist-induced activation of human FFA1 receptor signals to extracellular signalregulated kinase 1 and 2 through Gq- and Gi-coupled signaling cascades