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Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods

100%

Wojcik-Fatla A., Stojek N. M., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2012

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tom 19

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nr 2

The aim of the present study was: – to compare methods for concentration and isolation of Legionella DNA from water; – to examine the efficacy of various modifications of PCR test (PCR, semi-nested PCR, and real-time PCR) for the detection of known numbers of Legionella pneumophila in water samples artificially contaminated with the strain of this bacterium and in randomly selected samples of environmental water, in parallel with examination by culture. It was found that filtration is much more effective than centrifugation for the concentration of DNA in water samples, and that the Qiamp DNA Mini-Kit is the most efficient for isolation of Legionella DNA from water. The semi-nested PCR and real-time PCR proved to be the most sensitive methods for detection of Legionella DNA in water samples. Both PCR modifications showed a high correlation with recovery of Legionella by culture (p<0.01), while no correlation occurred between the results of one-stage PCR and culture (p>0.1).

2

Hantavirus RNA was not detected in Dermacentor reticulatus ticks

100%

Wojcik-Fatla A., Zajac V., Knap J. P., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2013

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tom 20

|

nr 3

A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.

3

Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland

100%

Cisak E., Zajac V., Wojcik-Fatla A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2012

|

tom 19

|

nr 3

4

Wystepowanie kretkow Borrelia burgdorferi i ich genogatunkow w kleszczach Ixodes ricinus zebranych z terenow rekreacyjnych Lubelszczyzny

100%

Cisak E., Chmielewska-Badora J., Wojcik-Fatla A.

Wiadomości Parazytologiczne. Suplement

|

2007

|

tom 53

139

5

The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR

100%

Sroka J., Szymanska J., Wojcik-Fatla A.

Annals of Agricultural and Environmental Medicine

|

2009

|

tom 16

|

nr 2

313-319

6

Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases

100%

Cisak E., Wojcik-Fatla A., Zajac V., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2012

|

tom 19

|

nr 4

7

Ocena wspolwystepowania Toxaplasma gondii i Borrelia burgdorferi w kleszczach Ixodes ricinus w oparciu o badania PCR

100%

Wojcik-Fatla A., Sroka J., Zajac V., Cisiak E.

Wiadomości Parazytologiczne

|

2010

|

tom 56

|

nr 3

207

8

Hantavirus RNA not detected in Ixodes ricinus ticks

100%

Wojcik-Fatla A., Zajac V., Knap J. P., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2011

|

tom 18

|

nr 2

9

Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources

100%

Stojek N. M., Wojcik-Fatla A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2012

|

tom 19

|

nr 2

A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.

10

Babesia microti w kleszczach Ixodes ricinus na terenie wojewodztwa lubelskiego

100%

Wojcik-Fatla A., Sroka J., Zwolinski J.

Wiadomości Parazytologiczne. Suplement

|

2007

|

tom 53

162

11

Badania w kierunku anaplazmozy u lesnikow z regionu Lubelszczyzny z uwzglednieniem badan srodowiskowych

100%

Chmielewska-Badora J., Zwolinski J., Cisak E., Wojcik-Fatla A.

Wiadomości Parazytologiczne. Suplement

|

2007

|

tom 53

137

12

Knowledge, protection behaviours and seroprevalence of Lyme borreliosis in inhabitants of Lublin Province, eastern Poland - evaluation of a prophylaxis programme

100%

Zajac V., Sroka J., Wojcik-Fatla A.

Annals of Agricultural and Environmental Medicine

|

2023

|

tom 30

|

nr 3

13

Occurrence of Toxoplasma gondii in water from wells located on farms

100%

Sroka J., Wojcik-Fatla A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2006

|

tom 13

|

nr 1

Among various species of parasitic protozoans which may contaminate drinking water, Toxoplasma gondii is of a special importance due to the high incidence of infections with this parasite noted in animals and humans. The objective of this study was to determine the frequency of occurrence of T. gondii in drinking water on farms in the area of the Lublin province (eastern Poland) with respect to health risk among the inhabitants, and to assess the role of water in the transmission of Toxoplasma infections in the rural environment. Studies were conducted on 87 farms located in the Lublin province, 14 of which were classified as possessing a good hygienic state, and 73 as possessing a poor hygienic state. A total number of 114 drinking water samples were taken, 80 samples from shallow household wells with a windlass, 16 from deep wells with a pump, and 18 from the water supply system. In microscopic and PCR examinations of 114 water samples, T. gondii was found in 15 (13.2%) and 31 (27.2%) of samples, respectively. The presence of T. gondii DNA detected by PCR test was found significantly more frequently in water samples from the shallow windlassoperated wells than in those from deep wells (p<0.05) and water supply system (p<0.01). Water samples collected from shallow wells located on farms of poor hygienic state contained significantly more frequently DNA of T. gondii than samples from shallow wells located on farms of good hygienic state (43.1% vs. 13.3%, p<0.05). In 26.3% of water samples, oocysts of other protozoans were found belonging to Isospora, Eimeria, and Cryptosporidium. Serologic examinations for the presence of anti-Toxoplasma antibodies conducted among 99 inhabitants of the farms where household wells were used showed 64.6% of seropositive results in IgG class antibodies and 1.0% in IgM class antibodies. Clinical cases of toxoplasmosis were also noted. In the total population examined, a positive correlation was observed between the consumption of unboiled well water and the presence of antibodies against T. gondii (p<0.05), this correlation being especially strong on farms of poor hygienic state enclosing shallow wells (p<0.001). In conclusion, the recorded presence of T. gondii in well water provides an evidence of the potential risk of waterborne infection for humans and animals. Therefore, it seems necessary to implement prophylactic actions on the endangered farms.

14

Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland

88%

Buczek A., Rudek A., Bartosik K., Szymanska J., Wojcik-Fatla A.

Annals of Agricultural and Environmental Medicine

|

2009

|

tom 16

|

nr 2

257-261

15

Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion

88%

Wojcik-Fatla A., Szymanska J., Wdowiak L., Buczek A., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2009

|

tom 16

|

nr 1

151-158

16

Missions of the Helicopter Emergency Medical Service in rural and urban areas in Poland - a comparative retrospective analysis

88%

Rzonca P., Galazkowski R., Wojcik-Fatla A., Panasiuk L., Gotlib J.

Annals of Agricultural and Environmental Medicine

|

2019

|

tom 26

|

nr 2

17

Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland

88%

Cisak E., Wojcik-Fatla A., Zajac V., Sroka J., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

|

2012

|

tom 19

|

nr 3

18

Biological agents as occupational hazards - selected issues

88%

Dutkiewicz J., Cisak E., Sroka J., Wojcik-Fatla A., Zajac V.

Annals of Agricultural and Environmental Medicine

|

2011

|

tom 18

|

nr 2

19

Fungal diversity of root vegetables and soil rhizosphere collected from organic and conventional farms in Eastern Poland

88%

Klapec T., Cholewa G., Cholewa A., Dutkiewicz J., Wojcik-Fatla A.

Annals of Agricultural and Environmental Medicine

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2018

|

tom 25

|

nr 2

20

Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces

88%

Sroka J., Karamon J., Dutkiewicz J., Wojcik-Fatla A., Cencek T.

Annals of Agricultural and Environmental Medicine

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2018

|

tom 25

|

nr 4

Ograniczanie wyników

Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods

Hantavirus RNA was not detected in Dermacentor reticulatus ticks

Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland

Wystepowanie kretkow Borrelia burgdorferi i ich genogatunkow w kleszczach Ixodes ricinus zebranych z terenow rekreacyjnych Lubelszczyzny

The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR

Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases

Ocena wspolwystepowania Toxaplasma gondii i Borrelia burgdorferi w kleszczach Ixodes ricinus w oparciu o badania PCR

Hantavirus RNA not detected in Ixodes ricinus ticks

Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources

Babesia microti w kleszczach Ixodes ricinus na terenie wojewodztwa lubelskiego

Badania w kierunku anaplazmozy u lesnikow z regionu Lubelszczyzny z uwzglednieniem badan srodowiskowych

Knowledge, protection behaviours and seroprevalence of Lyme borreliosis in inhabitants of Lublin Province, eastern Poland - evaluation of a prophylaxis programme

Occurrence of Toxoplasma gondii in water from wells located on farms

Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland

Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion

Missions of the Helicopter Emergency Medical Service in rural and urban areas in Poland - a comparative retrospective analysis

Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland

Biological agents as occupational hazards - selected issues

Fungal diversity of root vegetables and soil rhizosphere collected from organic and conventional farms in Eastern Poland

Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces