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The aim of the study was to analyse the annual changes in the concentration of free triiodothyronine, progesterone and testosterone in the serum of prepubertal red deer males and females. Blood was collected monthly from 8 stags and 8 hinds for a period of one year, beginning in November. Hormone concentrations were determined using an immunofluorescence method (TR-FIA). Monitoring of the duration of the prepubertal period, based on the progesterone concentrations in females and testosterone concentrations in the males, suggested that the prepubertal period ended in September in both sexes. The experiment revealed the annual rhythm in free triiodothyronine (FT3) secretion, with significantly higher concentrations in the winter-spring period in comparison with summer-autumn in both, males (P < 0.01) and females (P < 0.05). The results showed that the season considerably influenced the intensity of FT3 secretion in prepubertal red deer and that the secretion dynamics in both sexes were similar, however, the concentrations in the males reached higher levels than in the females in each season and across the whole experiment
The aim of this study was to determine circannual changes in the serum concentrations of thyroxine, calcitonin and parathormone in mature and immature red deer females. Blood samples from 8 hinds were collected monthly for 26 months. Secretions of thyroxine and calcitonin showed circannual rhythms with significantly higher levels in the immature hinds compared to the mature animals (p<0.05). For thyroxine, the concentration was higher in the winter/spring period than in summer/autumn (p<0.05), while for calcitonin the concentration profile was the opposite (p<0.05). The concentration of parathormone was significantly higher in summer/autumn that in the other months of the experiment (p<0.01). These results may indicate that the hormones investigated may be involved in the regulation of seasonal reproductive activity and in processes contributing to entering puberty in red deer females.
The aim of this study was to determine serum selenium concentrations in Polish Konik horses residing in the Odra Delta Nature Park (Poland) and to evaluate the activity of glutathione peroxidase and Se content in testes of this horse breed. In over 95% of cases, serum Se concentration was below the optimal range, and none of the horses examined was deficient in this trace element. The lack of Se deficiency in the animals examined suggests however, that the Polish Konik horses have a natural ability to the optimal use of nutrients available in their life area. Testicular content of Se and GSHPx activity in the colts was higher than those found in stallions, and a positive relationship between these antioxidants was demonstrated. The differences in Se contents and GSHPx activities in testes between colts and stallions suggest that selenoenzymes play important roles during the puberty of male horses.
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