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E-cadherin is an adhesion molecule playing a crucial role in the maintenance of epithelial tissue architecture. Deterioration of its functions, resulting in the loosening of intercellular linkages, may be conducive to neoplasm progression and metastasis formation. The aim of this study was to provide an immunohistochemical evaluation of E-cadherin expression in 6 sections of a normal canine mammary gland and in 8 benign and 36 malignant epithelial neoplasms, as well as to determine the relationship between the level of E-cadherin expression and the histological type and grade of tumour. In malignant tumours, a decreased level of E-cadherin expression was observed when compared to a normal gland and benign tumours, which depends on the histological type and malignancy grade. Moreover, a progressive decrease in E-cadherin expression was observed in mammary carcinomas with an increasing malignancy grade. This suggests that decreasing E-cadherin expression may contribute to the differentiation of poorly-differentiated neoplastic cells to an invasive phenotype, and may be one of the factors triggering the metastatic cascade.
The aim of the present study was to investigate the occurrence of Anaplasma spp. in group of 50 fallow deer (Dama dama) from free-range farm in eastern Poland and determine what species of Anaplasma could infect these animals based on PCR gene sequencing. The PCR technique revealed the presence of 16S RNA Anaplasma spp. genetic material in the blood of 7 out of 50 examined animals. The sequences of the PCR products obtained showed a 100% homology with each other and 100% homology with GU 183908 sequence of A. phagocytophilum, isolated in our earlier study from a horse with clinical form of anaplasmosis. Here, we report the first molecular evidence of Anaplasma spp. among naturally infected fallow deer in eastern Poland.
The experiment was performed on 36 Wistar rats. On the first day of the experiment iodoacetate was administered to the left posterior knee joint of the 18 rats which composed Group I. The second group of 18 rats received additionally doxycycline (doxy) through the gastric tube in doses comparable with those of doxycycline used in humans. The experiment lasted 21 days. The animals were sacrificed after 7, 14 and 21 days in groups of 6 rats each. In sections stained with Safranin 0 semiquantitative histochemical intensity tests were performed on articular cartilage glycosaminoglycans (GAG) using a four-point scale (0–3). In the first group examined destructive lesions in the articular cartilage and weak reactivity on GAG were noted at all stages of the experiment. The intensity of GAG staining was higher in the second group after 14 and especially after 21 days, which may suggest a protective action of doxy on articular cartilage.
The aim of this article was to describe cases of nasal tumors in dogs in which a rhinoscopy procedure was used as part of the process of disease diagnosis. The study included two dogs, aged 8 and 11 years, showing symptoms of epistaxis. The animals underwent a radiological examination and a rhinoscopy, during which bioptats were taken for histopathological examination. The radiological examination of the head did not reveal lesions characteristic of a neoplastic process. The rhinoscopic examinations showed a large hyperplasia closing the nasal canal in both dogs. The histopathological examination of the two bioptats sampled from the nose area demonstrated clusters of cells characteristic of a neoplastic process. The dogs were euthanized and subjected to a post-mortem examination. The histopathological examination of samples taken from the lesions in the nasal cavity confirmed olfactory neuroblastoma and transitional cell carcinoma in dogs 1 and 2, respectively. Rhinoscopy is a technique complementary to computer tomography, and, if the latter is impossible, it should represent, along with a radiological examination, the basis for a preliminary diagnosis of a neoplastic process, which ought to be confirmed by a cytological or histopathological examination of bioptats obtained from the sites of the lesions.
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