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Surface molecularly imprinted CdTe nanoparticles with molecular recognitive activity were prepared by reverse microemulsion polymerization with parathion as template molecules, 3-aminopropyltriethoxysilane and tetramethoxysilane as the polymerization precursors, and cross-linking. The determination method for parathion was developed on the basis of the fluorescence quenching of quantum dots caused by parathion due to the binding of molecularly imprinted cavities to parathion. The synthesized material had a distinguished selectivity and high binding affinity to parathion compared with chlopyrifos, diazinon, and pyrimithate. Under optimal conditions, the relative fl uorescence intensity of polymers decreased with increases of the concentration of parathion in the range 0.05-1000 μmol/L. The decreasing tendency of fluorescence intensity with increasing parathion concentration abides by the logistical growth curve with a detected limit of 0.218 μmol/L. This method was used to detect for parathion in water samples, for which recoveries ranging from 97.72% to 100.59% were obtained.
Foliar dust passively adsorbs anthropogenic heavy metals (HM) present in the atmosphere and thus reduces the total suspended particle (TSP) level. Urban plants have been shown to reduce the atmospheric level of ambient particulate matter (PM) via foliar dust adsorption. We studied heavy metal concentrations in the foliar dust of three typical tree species in five functional areas of Nanjing city. The highest levels of Cd (19.89±4.56 mg/kg), Pb (167.33±16.61 mg/kg) and Cr (197.42±13.96 mg/kg) were found in the Traffic Area (TA), whereas the highest levels of Cu (309.27±25.79 mg/kg) and Zn (1036.88±52.77 mg/kg) were found in the Industrial Area (IA). Significant differences were found between tree species. The amount of PM per unit leaf area generally decreased in this order: Cedrus deodara>Pittosporum tobira>Cinnamomum camphora. The highest mass percentages of large, coarse and fine PM were captured by C. camphora, P. tobira and C. deodara, respectively. A scanning electron microscope (SEM) was used to investigate the surfaces of the leaves, as well as the density and size of the stomata of each species. Our results suggest that an oily and coarse leaf surface is the most important factor facilitating PM accumulation, but large high-density stomata also enhance PM adsorption and thus favor HM accumulation in foliar dust. This study shows that the HM concentrations in foliar dust can act as an indicator of air pollution.
Aiming at the source of underwater acoustic emission, in order to identify the enemy emission sonar source accurately. Using the digital watermarking technology and combining with the good time-frequency characteristics of fractional Fourier transform (FRFT),this paper proposes a sonar watermarking method based on fractional Fourier transform. The digital watermark embedding in the fractional Fourier transform domain and combined with the coefficient properties of the sonar signal in the fractional Fourier transform to select the appropriate watermark position. Using the different characteristics of the signals before and after embedding, an adaptive threshold was set for the watermark detection to realize the discrimination of sonar signals. The simulation results show the feasibility and has better resolution and large watermark capacity of this method, while the robustness of the watermark is better, and the detection precision is further improved
The foliar surface of plants can capture atmospheric pollutants. Foliage dust is especially useful for passive adsorption of anthropogenic polycyclic aromatic hydrocarbons (PAHs) present in total suspended particles (TSPs). The objective of this study was to compare the dust-retaining capability of typical trees along an urbanization gradient in Nanjing, China. We also studied the concentrations of 16 PAHs in the foliage dust of four typical tree species. We concluded that the dust-retaining capability of the four typical tree species generally decreased in the order: Firmiana simplex > Symplocos sumuntia > Photinia serrylata > Osmanthus fragrans. The highest amounts of dust per unit leaf area were captured by F. simplex, and the mean values were 84.57, 63.11, and 56.29 μg·cm-2 in urban, suburban, and rural areas, respectively. PAH concentrations in foliage dust in urban areas were significantly higher than those in suburban and rural areas. Our results suggested that grooves surrounding the stomata and the distribution of tomentum over the leaf surface were the most important factors affecting the accumulation of dust, by facilitating the capture of fine dust particles, which tend to have higher PAH concentrations than larger particles. Scanning electron microscopy (SEM) of the leaf surface of F. simplex revealed that it was covered by tomentum, with grooves surrounding the stomata, and identified this species as a potential biomonitor for atmospheric pollution. From this study, it is evident that PAH concentration of foliage dust can act as indicator of air pollution.
Through the analysis of the residual activity of peroxidase (POD), chromatic aberration, shear intensity and shear power, the effects of different thermal treatment times at 100°C on the POD, surface colour and texture of Typha latifolia L. were evaluated. The results showed that the activity of POD decreased with the increasing thermal treatment time at 100°C. The regeneration amount of POD increased first for some time and then started to decrease with the treatment time. Thermal treatment times 1.0 and 1.5 min at 100°C exhibited maximum regeneration of POD for the samples stored at 20 and 37°C, respectively. The sample had acceptable texture and surface colour when they were treated at 100°C for 4 min because the POD in the sample was inactivated to an acceptable level.
Snowmelt hydrology becomes an important component of the soil and water assessment tool (SWAT) when spring flows are dominated by melting snow. The objective of this study was to evaluate the performance of the SWAT model’s snowmelt parameters by simulating nonpoint source pollution in the source area of the Liao River – a cold area in China. The seven snowmelt parameters for the simulation were adjusted. The exports rule of nonpoint source pollution for the spring snowmelt period was analyzed. The result show that SFTMP, SMTMP, SMFMX, SMFMN, and TIMP were sensitive parameters, while SNOCOVMX and SNO50COV were insensitive. In addition, two common statistics (Nash-Sutcliffe coefficient, and coefficient of determination) were used to evaluate the model. This evaluation indicated that the SWAT model had good performance simulating the seasonal nonpoint source pollution with large snowmelt. According to the analysis, rainfall and snowmelt is a main driver of nonpoint source pollution in the cold area, and that summer and spring are high-risk seasons. The study provides a new and suitable method of nonpoint source pollution simulation for the cold areas.
The occurrence of 22 antibiotics, including eight quinolones, nine sulfonamides and five macrolides in water and sediment from Zizhuyuan Lake has been investigated in Beijing, China. Sediment samples were extracted by pressurized liquid extraction (PLE). Aqueous extracts from PLE and water samples were concentrated by solid-phase extraction (SPE) and detected by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS). Antibiotics were detected in concentrations of up to 20 ng/L in water samples and 52.1 μg/kg in sediments. Quinolones were the most prevalent contaminants in water body, accounting for 63.4%, while quinolones and macrolides were prominent in sediments, accounting for 55.1% and 41.7%, respectively. The environmental risk assessment revealed that some antibiotics, in particular Ofloxacin (OFL) and Sulfamethoxazole (SMX), could cause high damage to algae and plants in the aquatic ecosystem.
This study investigated the neuronal protective effect of monosialotetrahexosylganglioside (GM1) on the hypoxia-ischemia white matter damage (WMD) in neonatal rats. Brain hypoxia-ischemia was induced by bilateral carotid artery occlusion in 4-day-old neonatal rats. Bilateral carotid artery occlusion (BCAO) was performed in rats in WMD and GM1 groups, while in sham group; the rat bilateral carotid arteries were merely exposed without occlusion. Immunohistochemical staining was used to determine the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), and P-amyloid precursor protein (P-APP). In addition, suspension test, slope test, and open-field test were carried out on day 26 after BCAO to determine the neurobehavioral function. The percentage of MBP-positive cells was decreased while P-APP-and GFAP- positive cells were increased in WMD group. After treated with GM1, the percentage of MBP-positive cells increased significantly than WMD rats at post-operation 72 h and day 7. GFAP-positive cells and P-APP-positive cells decreased significantly in WMD group at post-operation 72 h, day 7 and 26. The suspension test, slope test, and open-field test showed that neurobehavioral function was improved in ganglioside GM1 group compared with WMD group. Taken together, our findings suggested that ganglioside GM1 treatment reduces hypoxia-ischemia induced impairment of the neurobehavioral function in WMD in neonatal rats.
C-repeat binding factor (CBF) signaling pathway is involved in cold acclimation responsive to low temperature and some other stresses. CBF transcription factor family is the key component of this pathway. In this study, eight CBF-like genes, BrCBF1, BrCBF2, BrCBF3, BrCBF4, BrCBF5, and BrCBF6A/B/C were isolated from non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino, NHCC). The deduced CBF proteins shared high similarity with their Arabidopsis orthologs and localized to the nucleus. Furthermore, quantitative realtime PCR (qPCR) analysis showed that BrCBF1~3 were induced by cold (4 C) but not drought or abscisic acid (ABA), indicating that they are involved in an ABA-independent pathway; however, BrCBF4~6 were regulated by both drought and ABA, suggesting that they were involved in an ABA-dependent pathway. Nevertheless, unlike Arabidopsis, BrCBF4~6 showed response to both cold and ABA, indicates ABA-independent and ABA-dependent parts of CBF pathway in NHCC might not be completely separate, and these genes may act as the connection points in the network. BrCBFs were also accumulated in response to salicylic acid (SA), methyljasmonate (MeJA), and ethylene (ET), indicating that BrCBF genes might participate in the response to biotic stresses. Taken together, eight CBF genes were isolated from NHCC which compose a functional CBF signaling pathway by participating in response to multiple stresses and performing roles from Arabidopsis to some extent.
Urban plants have been proven to mitigate ambient particulate matter (PM), which can benefit urban planners in their attempts to control urban air pollution. In this study, PM depositions on the leaves of 8 tree species were quantitatively analysed in 7 functional areas of the city of Nanjing, China, over the course of one year. The results demonstrated that leaf PM included different particle size fractions (PM₁₀ and PM₂.₅), and differed among seasons and species. The highest amounts of total PM, PM₁₀, and PM₂.₅ were found in the industrial area, and the mean values were 80.24 μg/cm², 52.14 μg/cm², and 15.51 μg/cm², respectively, and the highest accumulation of total PM (60.65 μg/cm²), PM₁₀ (37.29 μg/cm²), and PM₂.₅ (11.23 μg/cm²) occurred in winter. Significant differences were found between the tree species tested. Cedrus deodara exhibited high amounts of the total PM, PM₁₀, and PM₂.₅ accumulations. This study examined the mass and quantity distribution of PM among tree species, and identified the particles combined with a scanning electron microscope (SEM). In terms of particle mass, 48% of the identified particles had a diameter of 10 μm, and only 18.3% of them had a diameter of 2.5 μm. In terms of particle number, the results indicated that 73% of them had a diameter of 2.5 μm, and only 5.5% of them had a diameter of 10 μm. To test the relationship between leaf traits and PM₂.₅ accumulation, results showed that stomata size, density, and hair were significantly related to the PM₂.₅ capture quantity. As far as we know, this is the first paper to present the mass and quantity distribution of the PM of different tree species in Nanjing. The results not only give comprehensive insights into the dust-retaining capability of tree species but also offer a selection of species for urban green areas where the goal is to mitigate urban airborne PM.
The plumule and coleoptile influence the emergence and vigor of the seedling and demonstrate distinct heterosis during maize (Zea mays L.) development. Comparative two-dimensional analysis was performed on plumules and coleoptiles from 3.5 days-cultivated seedlings of five widely used hybrids in China and their corresponding parental lines to dissect the genetic mechanism of heterosis. Compared to their parental lines, 64 % (2,127/ 3,310) of the differentially expressed proteins were nonadditively accumulated in the five hybrids. Up-regulated above the high parent, a similar expression pattern in all five tested hybrids, was the major expression pattern accounting for 68 % (1,446/2,127) nonadditive proteins. Forty-two nonadditive protein spots with significant differences between the hybrids and their parental lines were analyzed by mass spectra. Homology searches separated them into six groups with two abundant functional classes of cell detoxification (33 %) and metabolism (26 %), implying their importance for heterotic manifestation of the different hybrids at 3.5 days-cultivated seedling stage. The differential accumulation and expression of herbicide safener protein 1 in cell detoxification implied that exploration of the molecular mechanism of heterosis should be studied in a systematic network. Four potential functional polymorphisms were identified in SBP1 gene providing possible interpretation for the differential expression of SBP1 at transcriptional and translational levels.
The miR396 and growth-regulating factor (GRF) regulatory network is evolutionarily conserved among plant species. The orthologous GRF genes in rice were involved in regulating grain development, and targeted by miR396. However, there are still no more information about the involvement of miR396 and GRF genes in maize grain. In this article, RNA sequencing was performed on the developing maize kernels to analyze the dynamic profiles of miR396 and GRF genes and explore their possible regulatory roles during maize effective grain filling. Our results showed that GRF genes were also the conserved targets of miR396 in maize, according to computational prediction and validated by degradome sequencing. MiR396 expressed high and gradually declined with advancing maize grain filling, significant negatively correlated with its target GRF genes, which mostly increased continuously. Our test also provided a testimony of miR396-GRF network in different regulations between the development of maize embryo and endosperm by real-time quantitative PCR. And further analysis of expression pattern suggested that miR319, miR166, and RDR might interact with miR396-GRF network during grain development in maize. This study mainly provided a valuable foundation for future comprehensive analysis of miR396-GRF network, and further research work is needed to confirm the regulatory roles of miR396 and GRF genes during grain filling in maize.
The distribution of and prediction models for heavy metals in wheat were evaluated in Tianjin, China. The results indicated that there were high heavy metal concentrations (Zn, Cd, Cu, Pb, Cr, and Ni) in the wheat and that the Cd concentration (0.062mg·kg-1) in the seeds actually exceeded the national safety standard. The accumulation factors for the wheat’s seeds were of a decreasing order: Zn>Cd>Cu>Pb>Cr>Ni. The Cd concentration in the wheat’s seeds corresponded closely with the Cd concentration in the soil (r = 0.74). The predicting model was appropriate for predicting the concentration of Cd and Zn in wheat, and the r was 0.735 and 0.625, respectively.
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