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1

S-nitrosoglutathione modulates CXCR4 and ICOS expression

100%
Yamamoto Y., Pahwa R., Pahwa S.
Cellular and Molecular Biology Letters
|
2006
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tom 11
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nr 1
The expression of CXCR4, a membrane protein which is involved in the entry of HIV-1, is down-modulated from the cell surface by Phorbol 12-myristate 13-acetate (PMA) and the Ca+ ionophore, Ionomycin. Inducible co-stimulator (ICOS), which contributes to lymphocyte proliferation, is up-regulated by PMA/Ionomycin. We examined the influence of S-nitrosoglutathione (SNG), an inhibitor of Vacuolar H+-ATPase (V-ATPase), on the expression of CXCR4 and ICOS in PMA/Ionomycin-treated peripheral mononuclear cells (PBMC), and of CXCR4 alone in lymphoid cell lines. In this report, we show that SNG interferes with both effects of PMA/Ionomycin, namely CXCR4 down-regulation and ICOS up-regulation. These studies imply opposing roles of V-ATPase in the regulation of CXCR4 and ICOS. The influence of SNG in modulating the susceptibility of T cells to HIV-1 and on their immune responses needs further investigation.
2

Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy

76%
Yamanushi T. T., Boyett M. R., Yamamoto Y., Ohsaki H., Hirakawa E., Dobrzynski H.
Folia Morphologica
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2015
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tom 74
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nr 2
In this study, a fixation protocol using a 10% neutral buffered formalin (FA) solution and another protocol using a methanol (MeOH) solution were compared for detection of ion channels, Kᵥ 1.5, Kᵥ 4.2, Caᵥ 1.2, Kᵢᵣ6.2, Naᵥ 1.5 and Naᵥ 1.1 in rat myocytes by immunolabelling. Kᵥ 1.5 and Kᵥ 4.2 at intercalated discs and Caᵥ 1.2 at transverse tubules were not detected by FA but were detected by MeOH. Kᵢᵣ6.2 at transverse tubules and Naᵥ 1.5 at sarcolemma were detected by FA but not by MeOH. It is suggested that both FA and MeOH fixation protocols should be used for the detection of cardiac ion channels by immunolabelling. (Folia Morphol 2015; 74, 2: 258–261)
3

Al-induced secretion of organic acid, gene expression and root elongation in soybean roots

64%
Wu D., Zhao M., Shen S., Fu Y., Sasaki T., Yamamoto Y., Wei W., Shen H.
Acta Physiologiae Plantarum
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2013
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tom 35
|
nr 01
Al-activated organic acid transporter genes (ALMT and MATE) and plasma membrane H?-ATPase gene (PHA) are known to contribute to the regulation of organic acid secretion in several crops. However, it remains unclear how these genes interact to modulate organic acid exudation in the same plant species. In this study, Al-induced expression of genes (GmALMT1, GmMATE1 and GmPHA1), secretion of organic acid and root elongation were characterized in soybean roots. Results indicated that treatment with 50 lM Al activated the expression of GmALMT1, GmMATE1 and GmPHA1, and the exudation of citrate and malate significantly in apical 5 mm region of soybean seedlings, but inhibited root elongation by 57.8 %. The highest malate exudation rate and the maximal expression of GmALMT1 and GmPHA1 were observed after 2 h of 50 lM Al treatment, while the corresponding values for citrate exudation rate and GmMATE1 expression occurred at 8 h. The exudation of malate and citrate contributed to but could not recover Al-triggered root elongation. A root-split experiment indicated that Al-activated gene expression, organic acid secretion and root growth inhibition required the direct contact of Al3?. The removal of shoots in soybean seedlings decreased Al-activated gene expression by 26.1–40.5 %, and secretion of organic acid by 14.7–40.2 %. Furthermore, shoot excision aggravated Al-inhibited root elongation, indicating the existence of other Al tolerance mechanism except the exudation of organic acid. These results suggested that Al-activated expression of GmPHA1-, GmMATE1- and GmALMT1-mediated exudation of malate and citrate, and shoots played an important role in Al toxicity resistance in soybean roots.
4
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CD49d and CD49e induce cell adhesion-mediated drug resistance through the nuclear factor-kappaB pathway in Burkitt lymphoma

64%
Takeda, Tsubaki M., Genno S., Matsuda T., Yamamoto Y., Ueda E., Imano M., Satou T., Nishida S.
Journal of Physiology and Pharmacology
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2020
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tom 71
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nr 4
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