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1

Genetic variability and phylogenetic analysis of Lagovirus europaeus strains GI.1 (RHDV) and GI.2 (RHDV2) based on the RNA-dependent RNA polymerase (RdRp) coding gene

100%
Hukowska-Szematowicz B.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 1
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Changes in innate and adaptive immune response to Lagovirus europaeus GI.1 (Rabbit Haemorrhagic Disease Virus -RHDV) infection

100%
Hukowska-Szematowicz B.
Acta Biologica
|
2020
|
tom 27
3

Wybrane dane z zakresu biologii wirusa pomoru królików RHDV

63%
Hukowska-Szematowicz B., Deptula W.
Advances in Agricultural Sciences
|
2008
|
tom 12
4

Interferencja RNA - sposób na wyciszanie genów

63%
Hukowska-Szematowicz B., Deptula W.
Advances in Agricultural Sciences
|
2008
|
tom 12
5

Phylogenetic analysis of French strains of the RHD virus on the basis of a fragment of gene encoding C-terminal part of structural capsid VP60 protein

63%
Hukowska-Szematowicz B., Deptula W.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
6

Teoretyczne i praktyczne aspekty zjawiska interferencji RNA

63%
Hukowska-Szematowicz B., Deptula W.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 10
1191-1195
The discovery of the RNA interference phenomenon proved to be a significant breakthrough in determining the mechanism in which the flow of genetic information is controlled. Apart from dsRNA and siRNA, also microRNA molecules are involved in the process. Current data prove that the phenomenon of RNA interference will open new perspectives in the therapy of numerous diseases, including metabolic, cancer, neurodegenerative and viral ones.
7

Biologiczna rola mikroRNA [miRNA]- nowe dane

63%
Hukowska-Szematowicz B., Deptula W.
Postępy Biologii Komórki
|
2010
|
tom 37
|
nr 3
585-597
8

Non-specific cellular immunity in rabbits experimentally infected with four Czech strains of the rabbit haemorrhagic disease virus with different pathogenicity

63%
Hukowska-Szematowicz B., Deptula W.
Polish Journal of Environmental Studies
|
2012
|
tom 21
|
nr 4
The rabbit haemorrhagic disease (RHD) virus was first described in 1984 in China, where it caused a rabbit plague characterized by an acute course. At present, the disease has spread to rabbits on all continents, and is characterized by morality reaching 100%. Research on the immune response in rabbits after infection by RHD virus strains has so far only been performed by the Deptuła team. In turn, it must be stated that similar research worldwide has been performed in the Chinese centre, yet referring exclusively to rabbits after immunization with inactivated RHD virus. Such research indicates that shortly after immunization, the immunity is coordinated by macrophages and lymphocytes T and B, while farther on the protection against the infection is conditioned by humoral immunity. Deptula's team has investigated 22 strains of RHDV in the aspect of non-specific cellular and humoral immunity, as well as specific cellular and humoral immunity including 3 French strains (FR-1, Fr-2, 9905RHDVa), 10 Polish strains (K-l, Kr-1, KGM, SGM, MAŁ, BLA, PD, GSK, Ż, ŻD), 4 German strains (Hagenow, Frankfurt, Triptis, Hartmannsdorf), 3 Italian strains (BS89-reference strain, Vt97, PV97), 1 English strain (Rainham), and 1 Spanish strain (Asturias). The strains were analyzed in the aspect of such parameters as capacity of adherence and absorption of PMN cells, PMN cell cidal property measured with spontaneous, stimulated, and spectrophotometric NBT test, stimulation index and PMN metabolic activity coefficient; and MPO activity, as well as concentration and activity of LZM. Also, the number was marked of lymphocytes T CD5+, Th with receptor CD4+, Tc/Ts with receptor CD8+, and the number of lymphocytes with receptor CD25+, as well as the percentage of lymphocytes B (IgM). The research indicates the presence of immunogroups within the RHD virus. Assessment of pathogenicity of the RHD virus is actually performed based on the mortality rate in rabbits infected with the virus, which is dictated by the fact that the virus has so far not been obtained in vitro. Niedźwiedzka et al. divided the 10 analyzed strains into strains with high pathogenicity with mortality of 90-100%, up to 36/48 hour of the study (BS89, Hagenow, Rainham, Frankfart, Asturias, Triptis, Hartmannsdorf, Pv97, 9905RHDVa), and strains with lower pathogenicity with mortality of 30% up to 36/48h (Vt97). In turn, Tokarz-Deptuła divided the 10 analyzed strains of the RHDV (including 8 Polish and 2 French) into strains with mortality of 80-100% (Fr-2, ŻD, GSK, SGM, Fr-1, Kr-1, MAL), strains with mortality of 60-65% (KGM, BLA), and strains with mortality below 60% (PD). The aim of our study was to record changes to parameters of non-specific cellular immunity (capacity of adherence and absorption of PMN cells, cidal capacity of PMN cells measured with spontaneous NBT test, stimulated and spectrophotometric, and stimulation index and metabolic activity ratio of PMN cells) in rabbits experimentally infected with 4 haemagglutinogenic Czech strains of the RHD virus: CAMPV-351 (reference strain), CAMP-561, CAMPV-562, and CAMPV-558, with different pathogenicity; which strains have not yet been analyzed in this respect. The assessment of pathogenicity of the analyzed strains of the RHDV was performed on the basis of mortality rate among rabbits infected with these strains. On the basis of the number and duration of changes to analyzed parameters of non-specific cellular immunity, the 4 analyzed Czech strains are determined to differentiate in the aspect of immunogenicity into three groups. The first group is formed by the most immunogenic reference strain CAMPV-351, the second - by two medium-immunogenic strains - CAMPV-561 and CAMPV-558, whereas the third one - by the least immunogenic strain CAMPV- 562. The results obtained in the area of pathogenicity are not reflected in the division of the analyzed Czech strains according to their immunogenicity.
9
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MicroRNAs as new immunity regulators in viral and bacterial infections

63%
Szumna M., Hukowska-Szematowicz B.
Acta Biologica
|
2020
|
tom 27
10

Dynamics of peripheral blood lymphocytes in rabbits experimentally infected with two Czech strains of RHD virus

63%
Hukowska-Szematowicz B., Deptula W.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 1
Changes in the number of T and B lymphocytes and their subpopulations (Th, Tc/Ts, cells with CD25+ receptor) in peripheral blood of rabbits experimentally infected with one of two Czech strains (CAMPV-351 and CAMPV-561) of RHD virus were studied. The lymphocytes were identified using a cytometer and monoclonal antibodies directed against CD5+ (at T lymphocytes), CD4+ (at Th lymphocytes), CD8+ (at Tc/Ts lymphocytes), CD25, or IgM receptor at B lymphocytes. In addition, disease symptoms were recorded in the infected rabbits and their mortality was monitored. Analysis of the results showed that the examined strains induced a distinct immune response of the lymphocytes and that the response was more pronounced following the infection with the CAMPV-561 strain. The recorded changes were expressed more frequently by an increased than decreased percentages of the examined lymphocytes. The mortality was variable and reached 30% in animals infected with the CAMPV-351 strain and 75% in animals infected with the CAMPV-561 strain.
11

Genetic variability of czech and German RHD virus strains

51%
Hukowska-Szematowicz B., Pawlikowska M., Deptula W.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 3
237-245
RHD (rabbit haemorrhagic disease) virus (RHDV) is the aetiological factor of the haemorrhagic disease of rabbits and is currently present on all continents. RHDV is a small, envelope-free virus containing genetic material in the form of a 7437-nucleotide long RNA strand. Studies indicate that genetic variability of RDHV strains originating from various parts of the world is approximately 14%, regardless the time and place of isolation. The aim of this study was to evaluate the genetic variability of 6 RHD virus strains from the Czech Republic (CAMPV-561, CAMPV-562, CAMPV-558) and Germany (Frankfurt, Wika, Rossi) based on analysis of fragment of a gene coding a nonstructural p30 protein. The largest variability of nucleotide sequences within the studied fragment was found for the Rossi strain and CAMPV-562 (13.5%) and CAMPV-558 (13.5%), Wika and Frankfurt (12.1%), and CAMPV-561 and Wika (11.2%). Among the Czech strains the largest genetic distance was noted for strains CAMPV-558 and Iowa (0.130/0.140), and in the case of the German strains, for Frankfurt and Iowa (0.123/0.132). A homology tree constructed based on a fragment of ap30 protein-coding gene divided the 14 analysed strains into IV groups of 88% homology. Phylogenetic relationships also divided the tested strains into 4 genetic groups (G1-G4). The larger genetic distance exists between the Czech and German strains and the American ones, and the smallest between them and the European strains.
12

Phylogenetic analysis of selected strains of rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60

51%
Pawlikowska M., Hukowska-Szematowicz B., Deptula W.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 2
129-133
The purpose of the study was the phylogenetic analysis of 16 European strains of the rabbit haemorrhagic disease (RHD) virus identified in 1989-2004, on the basis of N-terminal fragment of the gene encoding structural protein VP60. The obtained sequences of the strains were compared to the sequences of 30 strains of the virus received from the GenBank gene database. As a result of phylogenetic analysis of all the strains, they were divided into six genogroups, which were principally formed on the basis of the time of their identification, and the place of isolation of the RHD virus, which confirms the present hypothesis of genogroups creation among this virus. Furthermore, in our studies, a clear and visible difference was obtained between the classic strains in genogroups 1-5, and the strains referred to as antigen variants - genogroup 6.
13

Comparative analysis of the gene encoding RNA-dependent RNA polymerase in RHD virus strains originating from various continents

51%
Hukowska-Szematowicz B., Lejkowska R., Deptula W.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
14

Comparative analysis of the gene encoding VP60 capsid protein in various strains of the RHD (rabbit haemorrhagic disease) virus

51%
Hukowska-Szematowicz B., Manelska A., Tokarz-Deptula B., Deptula W.
Advances in Agricultural Sciences
|
2011
|
tom 14
|
nr 1-2
15

Analysis of genetic variability and phylogenetic analysis of selected Czech and French strains of rabbit haemorrhagic disease virus (RHDV)

51%
Hukowska-Szematowicz B., Tokarz-Deptula B., Deptula W.
Journal of Applied Genetics
|
2013
|
tom 54
|
nr 2
The objective of this study was to analyse the genetic variability and phylogenetic analysis of six strains of rabbit haemorrhagic disease virus (RHDV), including four Czech strains (CAMPV-351, CAMPV-561, CAMPV- 562, CAMPV-558) and two French strains (Fr-1, Fr-2), on the basis of a fragment of the VP60 capsid structural protein-coding gene N-terminal region. The results of our own studies were compared to 26 RHDV strains obtained from GenBank. The analysis of the genetic variability of six RHDV strains indicated that the CAMPV-561 strain is the most genetically variable. Less variable were the Fr-1 and Fr-2 strains, while the least variable was CAMPV-351. In turn, the genetic distance among the six analysed strains and 26 strains obtained from GenBank was the greatest for CAMPV-351 and Whn/China [11.3 % according to the observed divergence (OD) method and 12.2 % according to the maximum likelihood (ML) method], while it was the lowest for CAMPV-351 and FRG (0.8 % in both the OD and ML methods). In turn, the scale of the genetic distances among the six analysed strains and five RHDVa strains (99-05, NY-01, Whn/China, Triptis, Iowa2000) ranged from 9.3–10.3 % in the OD method to 10.3–13.7 % in the ML method. The image of phylogenetic dependencies generated for the strains analysed and those obtained from GenBank revealed their distribution to be in five genetic groups (G1–G5), whereas the analysed strains were included in genetic groups 2 and 3.
16

Wskazniki odpornosci u krolikow eksperymentalnie zakazonych szczepami wirusa RHD o odmiennych wlasciwosciach hemaglutynacyjnych

51%
Tokarz-Deptula B., Niedzwiedzka P., Hukowska-Szematowicz B., Deptula W.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 10
1251-1254
The aim of the study was to monitor alterations in selected indices of innate immunity (the phagocytic index and percent of phagocytes) and acquired immunity (numbers of lymphocytes T and their Th and Tc/Ts subpopulations) in 60 rabbits experimentally infected with haemagglutinogenic Czech strain CAMP V-351 and non-haemagglutinating Polish BLA. The haemagglutinogenic Czech strain CAMP V-351 is active only in select indices of innate immunity, whereas non-haemagglutinating Polish strain BLA is reactive in the parameters of acquired immunity. Therefore, the obtained results permit the conclusion that the examined strains are not only serologically different but they also induce distinct patterns of immune responses, which provides evidence of immunotypes among this virus.
17

Genetic variation and phylogenetic analysis of rabbit haemorrhagic disease virus (RHDV) strains

51%
Hukowska-Szematowicz B., Tokarz-Deptula B., Deptula W.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 4
 Rabbit haemorrhagic disease virus (RHDV) belongs to the family Caliciviridae and is the etiological agent of the haemorrhagic disease, also known as rabbit plague. Its genome is a linear single-stranded (ss) RNA of 7437 nucleotides and the capsid is built from a single structural protein VP60. In connection with the discovery of new RHDV strains, there is a constant need to investigate the genetic variation of this virus and perform phylogenetic analyses which may show the evolutionary relationships among the RHDV strains. Studies on the divergence of RHDV have shown that it is genetically quite stable, although recent observations indicate that some new RHDV strains, significantly different from the original RHDV subtype and the new RHDVa subtype, are appearing. These latest findings suggest that a new group of RHDV strains has evolved. The present review summarizes the current knowledge on the genetic variation and the latest achievements in phylogenetic analyses of RHDV strains isolated in various countries.
18
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Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle

45%
Tokarz-Deptula D., Hukowska-Szematowicz B., Niedzwidzka-Rystwej P., Trzeciak-Ryczek A., Deptula W.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
The objective of the study was to determine values of apoptosis for peripheral blood lymphocytes and granulocytes, including dependency on the season of the year, in Polish mixed-breed rabbits and in mixed-breed rabbits with the addition of blood of meat-breed rabbits. The study was carried out in four seasons of the year (spring, summer, autumn, winter) involving 120 Polish mixed-breed rabbits – group I, and 120 Polish mixed-breed rabbits with addition of meat-breed rabbit blood – group II. Assessment of apoptosis of lymphocytes and granulocytes was performed using as FACScan cytometer by Becton Dickinson with FACSDiva software (USA), using as ApoFluor®Green Caspase reagent kit (MP Biomedicals, USA) to detect the activity of the total caspase pool 1, 3, 4, 5, 6, 7, 8, 9 in granulocytes and lymphocytes of rabbit peripheral blood. The results for apoptosis of lymphocytes and granulocytes in peripheral blood in the animals investigated (group I and II) were subjected to statistical analysis with the t’Student test at p=0.05. It was noted that, in rabbits from group I, the values for apoptosis of lymphocytes were the highest in winter and autumn (36.02% and 31.24%, respectively), and the lowest in spring and summer (26.73% and 22.72%, respectively), whereas in the case of granulocytes the highest values were in summer and spring (14.69% and 12.95%, respectively), and the lowest in winter and autumn (8.16% and 8.57%, respectively). In mixed-breed rabbits with the addition of meat-breed blood (group II), the values for apoptosis of lympocytes were the highest in spring (29.13%), and the lowest in summer (25.43%); whereas in the case of granulocytes the highest values were in summer and spring (14.0% and 11.15%, respectively), and the lowest in autumn and winter (7.46% and 7.64%, respectively).
19

Próba opracowania norm w zakresie wskaźników biało- i czerwonokrwinkowych u krajowych królików mieszańców

45%
Dzialo J., Tokarz-Deptula B., Hukowska-Szematowicz B., Niedzwiedzka-Rystwej P., Deptula W.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2011
|
tom 48
20
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Content available

Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV

45%
Niedzwiedzka-Rystwej P., Hukowska-Szematowicz B., Dzialo J., Tokarz-Deptula B., Deptula W.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 1
The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits – RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains’ features.
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