Wyniki wyszukiwania

1

The identification and characterization of a testis-specific cDNA during spermatogenesis

100%

Chen Y., Hu J., Song P., Gong W.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 1

Using bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RT-PCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse tests, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.

2

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Loop-mediated isothermal amplification as a good tool to study changing Leptosphaeria populations in oilseed rape plants and air samples

76%

Jedryczka M., Burzynski A., Brachaczek A., Langwinski W., Song P., Kaczmarek J.

Acta Agrobotanica

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2013

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tom 66

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nr 4

LAMP is an innovative, simple, rapid, specific and cost- -effective nucleic acid amplification method. Due to the use of a special enzyme – GspSSD polymerase, the reaction takes a short time and can be performed at isothermal conditions. The sensitivity and specificity of LAMP technique is significantly higher, than standard PCR techniques, as two or three specific primer pairs are used. The technique is regarded as a useful tool for the detection and identification of plant pathogens. In this work, LAMP was used to study the composition of the population of fungi of the genus Leptosphaeria, causing a damaging disease of oilseed rape, called blackleg or stem canker. The detection concerned DNA present in fungal spores contained in air samples obtained using Hirst-type volumetric trap, in Pomerania (north Poland) in 2010. The results achieved using the LAMP technique were similar to these obtained with previously used, highly specific method of Real-time PCR. Conducting LAMP reaction was much easier and less time-and cost-consuming, due to a simplified method of DNA isolation of pathogens from plant tissues. Then, the LAMP technique was used to assess the composition of the population of Leptosphaeria spp. in plants of oilseed rape collected from the field in the Opole region (south-western part of Poland) in 2013. In contrast to studies conducted in 2002–2003, the analysis of leaf symptoms showed a higher proportion of L. maculans compared to L. biglobosa, what reflects changes in the composition of pathogen population of fungi causing blackleg on oilseed rape in this part of Poland.

3

Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus

76%

Song P., Chen X., Wu B., Gao L., Zhi H., Cui X.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 06

4

Structural predication and antigenic analysis of Toxoplasma gondii ROP20

52%

Zhou J., Wang W., Song P., Wang L., Han Y., Guo J., Hao Z., Zhu X., Zhou Q., Du X., Lu G., He S., Luo Y.

Acta Parasitologica

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2018

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tom 63

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nr 2

Ograniczanie wyników

1 Acta Agrobotanica

1 Acta Parasitologica

1 Acta Physiologiae Plantarum

1 Cellular and Molecular Biology Letters

4 Song P.

1 Brachaczek A.

1 Burzynski A.

1 Chen X.

1 Chen Y.

1 Cui X.

1 Du X.

1 Gao L.

1 Gong W.

1 Guo J.

1 Han Y.

1 Hao Z.

1 He S.

1 Hu J.

1 Jedryczka M.

1 Kaczmarek J.

1 Langwinski W.

1 Lu G.

1 Luo Y.

1 Wang L.

1 Wang W.

1 Wu B.

1 Zhi H.

1 Zhou J.

1 Zhou Q.

1 Zhu X.

1 2018

1 2016

1 2013

1 2006

The identification and characterization of a testis-specific cDNA during spermatogenesis

Loop-mediated isothermal amplification as a good tool to study changing Leptosphaeria populations in oilseed rape plants and air samples

Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus

Structural predication and antigenic analysis of Toxoplasma gondii ROP20