Wyniki wyszukiwania

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In vitro and in vivo gene transfer into neural cells using HSV1 and polyethylene imine (PEI)

100%

Tabbaa S., Goulah C., Tran R., Torres G., Horowitz J. M., Bloom D. C., Stachowiak M. K.

Acta Neurobiologiae Experimentalis

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1999

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tom 59

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nr 3

2

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

100%

Tabbaa S., Corso T. D., Jenkins L., Tran R. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

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2005

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tom 64

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nr 4

273-281

This study shows that an ICP4– replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the β-galactosidase gene can be used as a very effective vector for delivering the β-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed β-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.

3

Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods

68%

Corso T. D., Torres G., Goulah C., Roy I., Gambino A. S., Nayda J., Buckley T., Stachowiak E. K., Bergey E. J., Pudavar H., Dutta P., Bloom D. C., Bowers W. J., Stachowiak M. K.

Folia Morphologica

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2005

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tom 64

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nr 3

130-144

CNS gene transfer could provide new approaches to the modelling of neurodegenerative diseases and devising potential therapies. One such disorder is Parkinson’s disease (PD), in which dysfunction of several different metabolic processes has been implicated. Here we review the literature on gene transfer systems based on herpes simplex virus type 1 (HSV-1) and non-viral polyethyleneimine (PEI) and calcium phosphate nanoparticle methods. We also assess the usefulness of various CNS gene delivery methods and present some of our own data to exemplify such usefulness. Our data result from vectors stereotaxically introduced to the substantia nigra (SN) of adult rats and evaluated 1 week and/or 1 month post injection using histochemical methods to assess recombinant β-galactosidase enzyme activity. Gene transfer using PEI or calcium phosphate-mediated transfections was observed for both methods and PEI was comparable to that of HSV-1 amplicon. Our data show that the amplicon delivery was markedly increased when packaged with a helper virus and was similar to the expression profile achieved with a full-size replication-defective HSV-1 recombinant (8117/43). We also examine whether PEI or HSV-1 amplicon-mediated gene transfer could facilitate assessment of the biological effects induced by a dominant negative FGF receptor-1 mutant to model the reduced FGF signalling thought to occur in Parkinson’s disease.

4

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

68%

Tabbaa S., Goulah C., Tran R. K., Lis A., Korody R., Stachowski B., Horowitz J. M., Torres G., Stachowiak E. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

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2000

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tom 59

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nr 4

Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors have been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS.

Ograniczanie wyników

3 Folia Morphologica

1 Acta Neurobiologiae Experimentalis

4 Bloom D. C.

4 Stachowiak M. K.

3 Goulah C.

3 Tabbaa S.

3 Torres G.

2 Corso T. D.

2 Horowitz J. M.

2 Stachowiak E. K.

2 Tran R. K.

1 Bergey E. J.

1 Bowers W. J.

1 Buckley T.

1 Dutta P.

1 Gambino A. S.

1 Jenkins L.

1 Korody R.

1 Lis A.

1 Nayda J.

1 Pudavar H.

1 Roy I.

1 Stachowski B.

1 Tran R.

2 2005

1 2000

1 1999

In vitro and in vivo gene transfer into neural cells using HSV1 and polyethylene imine (PEI)

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors