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Heavy metals pollution of surface soils is a major global issue. To assess the ecological risk caused by heavy metals, 280 samples were collected in Chiping, Shandong Province, China. Eight different heavy metals in surface soils (Cu, Pb, Cd, Cr, Hg, Ni, Zn, and As) were analyzed in this study. The author got the realization of the eight kinds of heavy metals respectively based on sequential simulation methods. Next, the ecological risk of the region of heavy metals was assessed using the Hakanson potential danger index. The result showed that Hg was the main heavy metal problem in ChiPing. The potential ecological risk caused by Hg amounts to 200 on the Hakanson potential danger index, which was classified as ‘high.’ Cd has a potential ecological risk of nearly 72, which was classified as ‘medium.’ The comprehensive potential ecological risk caused by all eight different heavy metals was 278.34. Therefore, the heavy metal pollution of the study area was associated with a ‘medium’ potential ecological risk. Finally, the result was analyzed based on the land use map. We found that the highest integrated potential ecological risk area was located in plough land.
The findings of preserved soft body parts including pedicle in the linguloid brachiopods are extremely rare in the fossil record of which the early Cambrian Chengjiang (southern China) and Burgess Shale (British Columbia) faunas are the most important. However, these characteristic Cambrian soft−bodied faunas largely disappeared from the fossil re− cord well before the end of the Cambrian. Here we describe the first record of the pedicle in a linguloid brachiopod from the post−Cambrian strata, preserved with remarkable fidel− ity. Contrary to the Chengjiang and Burgess Shale−type fau− nas which are commonly preserved as essentially two−di− mensional aluminosilicate or degraded organic carbon films or pyritized compressed fossils, the specimens now recov− ered from the Ordovician of China show a three−dimen− sional, pyritized pedicle with preserved external morphol− ogy, in detail. The presence of streamlined shell shape and burrowing shell sculpture in our specimens supports an as− sumption for infaunal mode of life of the genus. Since all linguloid brachiopods of the early Cambrian are inter− preted as epifaunal or semi−infaunal, it seems that the here described Ordovician linguloid is the oldest representative of fully infaunal brachiopods. Apparently, the long vermi− form and flexible linguloid pedicle has appeared as a func− tionally optimized construction.
The shallow water assemblage of chondrichthyan microremains, teeth, tooth plates and scales, from the middle Tournaisian (Mississippian) of the vicinity of Muhua village, Guizhou province, southern China, is thus far the richest and most diverse association of this age collected from a single locality and horizon, and represents a chondrichthyan community very restricted in time and space. It was recovered from a small bioclastic limestone lens, MH−1, occurring among basinal marls near the base of the Muhua Formation, and dated as to the Siphonodella crenulata conodont Zone. The majority of the fauna presented here consists of teeth with euselachian−type bases and crushing crowns belonging to bottom−dwelling durophagous chondrichthyans, most probably feeding on shelly invertebrates such as the abundant brachiopods. We assigned most of these teeth to Euselachii (six species, among them Cassisodus margaritae gen. et sp. nov.), Petalodontiformes (two species), Holocephali (five species), and Euchondrocephali incertae sedis (Cristatodens sigmoidalis gen. et sp. nov.). We also identified primitive polycuspid, clutching teeth representing Phoebodontiformes (Thrinacodus bicuspidatus sp. nov.), Symmoriiformes, and Ctenacanthiformes. The scales are typical growing, compound forms of the protacrodont, ctenacanth, and hybodont types. Two problematic denticulated plates were found, one of which resembles mandibular or palatal plates of Sibyrhynchus (Iniopterygii). Several of the identified chondrichthyan taxa have hitherto been known only from Laurussia, especially from the British Isles and central USA. In particular we found the first record of Chondrenchelyssp. and Diclitodus denshumani outside their type locality. Th. bicuspidatus sp. nov., also known from Nevada, Iran, and NW Australia, appears to be a cosmopolitan, middle Tournaisian index fossil.
Evidence of brachiopod shell infestation by tube dwelling parasitic–commensal organisms is very rare in the fossil record. The oldest record of this kind of biotic interaction is known as Eodiorygma acrotretophilia from the Early Cambrian phosphatic acrotretoid Linnarsonia. The youngest evidence of parasitic infestation was documented in the Early Cretaceous rhynchonellide Peregrinella multicarinata. Two other records of vermiform tubes inside brachiopod shells come from the Devonian. These are Diorygma atrypophilia, infesting Givetian atrypide shells, and Burrinjuckia spiriferidophilia, found in some Emsian spiriferides. Here we describe the fifth record of this kind of infestation for which a name Haplorygma dorsalis ichnogen. et ichnosp. nov. is proposed. The tubular infestation structure was revealed in two silicified dorsal valves of spirolophous brachiopods found in the Mississippian Muhua Formation of the Southern China. The affinity of the tube−dwelling organism is rather enigmatic, but its annelid relationship and kleptoparasitic nature seems highly probable. In addition, the phoronid affinity of Diorygma is here questioned.
Helicosporidia are gut parasites of invertebrates. These achlorophyllous, non-photosynthetic green algae are the first reported to infect insects. Helicosporidia are members of the green algal class Trebouxiophyceae and are further related to the photosynthetic and non-photosynthetic genera Auxenochlorella and Prototheca, respectively, the latter of which can also turn to parasitism under opportunistic conditions. Molecular analyses suggest that Helicosporidia diverged from other photosynthetic trebouxiophytes less than 200 million years ago and that its adaptation to parasitism is therefore recent. In this minireview, we summarize the current knowledge of helicosporidian genomics. Unlike many well-known parasitic lineages, the Helicosporidium sp. organelle and nuclear genomes have lost surprisingly little in terms of coding content aside from photosynthesis-related genes. While the small size of its nuclear genome compared to other sequenced trebouxiophycean representatives suggests that Helicosporidium is going through a streamlining process, this scenario cannot be ascertained at this stage. Genome expansions and contractions have occurred independently multiple times in the green algae, and the small size of the Helicosporidium genome may reflect a lack of expansion from a lean ancestor state rather than a tendency towards reduction.
The second part of the monograph of the silicified brachiopod fauna from the Muhua Formation concludes with the descriptions of 36 species belonging to 32 genera and 22 families. Eighteen species are reported in open nomenclature. Two new rhynchonellide species are described: Coledium bruntoni sp. nov. and Pleuropugnoides calcaris sp. nov. The described brachiopod fauna is dominated by spiriferides (16 species), rhynchonellides (9 species), and athyridides (7 species), while spiriferinides and terebratulides are represented by 1 and 3 species, respectively. The brachiopod fauna from the Muhua Formation is characterised by remarkably high species diversity. Together with those species described in the first part of the monograph the fauna includes 69 species. The study of the brachiopod faunal dynamics during the late Famennian–late Tournaisian in southern China reveals that after a decline in the generic diversity at the Devonian–Carboniferous boundary (D–C boundary event), the Early Tournaisian brachiopod fauna shows slight impoverishment. In the middle Tournaisian the brachiopod fauna from South China shows an explosive increase in diversity on generic level which is well exemplified by the material from Muhua. The brachiopod fauna from the Muhua Formation represents a fully recovered high diversity fauna consisting of forms representing a wide spectrum of attachment strategies as well as highly specialised forms (e.g., micromorphs) adapted to special kinds of ecological niches. Numerous evidence of the biotic interaction between brachiopods and other co−occurring fauna have been revealed in the material from Muhua. These are drill holes of predatory origin, borings made on dead shells as post−mortem infestation, shell damages and malformations, and parasitic infestations.
This paper describes 37 species (4 new) belonging to 27 genera (1 new), 14 families, and 6 orders (Lingulida, Craniida, Strophomenida, Productida, Orthotetida, and Orthida) of silicified brachiopods from the middle Tournaisian (Mississippian, lower Carboniferous) of the vicinity of Gedongguan and Muhua villages (southern China). All specimens come from acid etching of detrital and oolitic limestone lenses scattered within grey to black laminated basinal micrite and marl of the Muhua Formation. The formation, which attains about 1–10 meters in thickness, is dated to the Siphonodella crenulata Zone. More than 10,000 silicified brachiopod specimens belonging to about 70 species were recovered from over 900 kg of the sampled limestone lenses, the most diverse brachiopod fauna of that age. The characteristic feature of the studied material is the prevailing disarticulation and fragmentation of skeletal parts due to their down slope transportation into a deeper water environment. Within Lingulida, one linguloid and one discinoid species are described. Craniida are represented by five species including Nematocrania pilea sp. nov. Strophomenida are represented by fragmentarily preserved specimens belonging to one species. The most diverse are Productida, which are represented by 7 chonetidine and 12 productidine species; new are Subglobosochonetes gedongguanensis sp. nov. and Globosochonetes gracilis sp. nov. New data on morphology of larval stage of Argentiproductus margaritaceus and its mode of attachment are presented. Orthotetida is represented by 5 species including Lamellispina spinosa gen. et sp. nov. Orthida is represented by 3, mostly cosmopolitan species. This study of a middle Tournaisian brachiopod fauna from Muhua, together with published data on the Mississippian brachiopods from other regions of South China, allow to study the Devonian–Carboniferous biotic crisis and post−crisis recovery.
Six silicified left valves including one nearly complete were obtained from acid residues of two samples collected from the Early Carboniferous Muhua Formation in Guizhou Province, China. The Chinese specimens have reticulate ornament and a large asymmetrical aviculopectinid−type resilifer and are much like Girtypecten from Permian rocks in the United States. However, the Chinese material shows only unicostate radial ornament, whereas Girtypecten is multicostate. Thus, a new taxon Girtypecten (Sinopecten) newelli subgen. et sp. nov. is proposed. The conodont assemblage occurring with G. (Sinopecten) newelli dates the new scallop as Tournaisian (the conodont Lower Siphonodella crenulata Zone); this is the oldest known occurrence of Girtypecten.
This experiment was designed to determine the relationship between the ultrastructure of the embryo cells and the changes in antioxidant enzymatic activities and lipid peroxidation in oat (Avena sativa L.) seeds with different moisture contents (4, 10 and 16 %) that were aged for 0, 8, 16, 24, 32 and 40 days in 45 C. The results showed that the declining in the germination percentage and integrity of cell ultrastructure in oat aged seeds were presented during the aged process, and these changes would be enhanced by the higher moisture content. There were consequent changes for biochemical reactions and lipid peroxidation exhibited. For oat seeds with 4 and 10 % moisture content, SOD and CAT were much more sensitive than APX at the early stages of imbibition after mild ageing, the activities of SOD, CAT, APX and MDHAR at 4 h of imbibition decreased significantly (P\0.05) after being aged from 32 to 40 days. On the contrary, MDA and H2O2 contents both did not increase further. Upon imbibition the activities of DHAR and GR increased after mild ageing, declined after further ageing and maintained a stable level after ageing from 24 to 40 days at those moisture levels. Both activities were higher after ageing at 4 % moisture content than at 10 %. The decline in integrity of ultrastructural cells was related with accumulation of H2O2 during seed ageing, and favoured by the decrease of SOD, CAT, APX and MDHAR activities after imbibition. The activities of antioxidant enzymes and contents of MDA and H2O2 in oat seeds with 16 % moisture content all gradually decreased after ageing from 8 to 40 days, also the ultrastructure of embryo cells was severely damaged. Its ultrastructure was destroyed much more quickly in the seeds with higher moisture content. The level of moisture content could accelerate the seed deterioration, and mitochondrial damages were probably the main reason for oat seed ageing. However, the activities of antioxidant enzymes were the key factor to repair the damage from lipid peroxidation and to maintain the integrity of cell ultrastructure for oat aged seeds during imbibition.
Soil erosion in the Pisha sandstone area of the Loess Plateau in China has become a severe environment issue that has raised concerns globally. The projects of ecological restoration in this area and their impact on soil erosion have been analyzed using the unmanned aerial vehicle remote sensing system (UAVRSS) and the revised universal soil loss equation (RUSLE) in the Two-Tiger Valley Basin in 2013 and 2015. Our findings show that: 1) The volume of soil erosion and average soil erosion modulus decreased from 126.24 t year-1 and 6465.295 t km-2 year−1 to 114.7 t year-1 and 6333.19 t km-2 year−1 between 2013 and 2015, respectively. 2) Spatial-temporal variations of soil erosion are extremely significant. All erosion grades recorded different degrees of decline across the study period, except for the level of severe erosion. 3) There is a significant positive correlation between slope degree and soil erosion. When the slope degree was <5°, the soil erosion modulus was 51.355 t km-2 year−1, accounting for only 0.87 % of total erosion in this area. When the slope degree was >35°, the soil erosion modulus attained 2574.413 t km-2 year−1, and the erosion amount accounted for 43.52% of total erosion. Although anti-erosion and the promotion of plant growth measures have achieved noticeable ecological benefits, the present situation of preventing and controlling soil and water loss is still severe.
An obligately thermophilic strain ZY-10 was isolated from the crude oil in a high-temperature oilfield, which was capable of degrading heavy crude oil. Phenotypic and phylogenetic analysis demonstrated that the isolate should be grouped in the genus Geobacillus, which shared the highest similarity (99%) of the 16S rDNA sequence to Geobacillus stearothermophilus. However, the major cellular fatty acid iso-15:0 (28.55%), iso-16:0 (24.93%), iso-17:0 (23.53%) and the characteristics including indole production, tolerance to NaN₃ and carbohydrate fermentation showed some difference from the recognized species in the genus Geobacillus. The isolate could use tridecane, hexadecane, octacosane and hexatridecane as sole carbon source for cell growth, and the digesting rate of long-chain alkane was lower than that of short-chain alkane. When the isolate was cultured in the heavy crude oil supplement with inorganic salts and trace yeast extract, the concentration of short-chain alkane was significantly increased and the content of long-chain alkane was decreased, suggesting that the larger hydrocarbon components in crude oil were degraded into shorter-chain alkane. Strain ZY-10 would be useful for improving the mobility of crude oil and upgrading heavy crude oil in situ.
Liverworts are known to be a rich source of terpenoids and phenolic compounds whose biochemical and molecular biosynthesis properties are not well understood. To evaluate the biosynthetic processes of such compounds found in a liverwort Plagiochasma appendiculatum, a total of 5,024 clones were sequenced from a normalized cDNA library from leaves of P. appendiculatum. This produced 4,384 high-quality ESTs with a mean length of 550 bp. Cluster analysis indicated the presence of 704 contigs and 2,720 singletons, generating 3,424 unique sequences. A total of 1,180 sequences were functionally classified using gene ontologies (GO). Based on the homology to sequences present in GenBank, our EST collection was found to contain orthologs for known prenyl transferases and for genes involved in the 2-C-methyl-Derythritol 4-phosphate (MEP) and the mevalonic acid (MVA) pathways, both of which are involved in terpenoid biosynthesis. Informatic analysis revealed that all of the genes involved in terpenoid biosynthesis showed extensive homology with tracheophyte genes. Phylogenetic analysis indicated that the topology of the phylogenetic trees constructed using genes MEP, DXR and HMGS was in good agreement with the traditional taxonomic classification, but the one constructed using gene FPS was not. Real-time PCR showed that the expression level of these genes was enhanced after the MeJA treatment, as in vascular plants.
Stomatal behavior in response to drought has been the focus of intensive research, but less attention has been paid to stomatal density. In this study, 5-week-old maize seedlings were exposed to different soil water contents. Stomatal density and size as well as leaf gas exchange were investigated after 2-, 4- and 6-week of treatment, which corresponded to the jointing, trumpeting, and filling stages of maize development. Results showed that new stomata were generated continually during leaf growth. Reduced soil water content significantly stimulated stomatal generation, resulting in a significant increase in stomatal density but a decrease in stomatal size and aperture. Independent of soil water conditions, stomatal density and length in the trumpeting and filling stages were greater than in the jointing stage. Irrespective of growth stage, severe water deficit significantly reduced stomatal conductance (Gs), decreasing the leaf transpiration rate (Tr) and net photosynthetic rate (Pn). Stomatal density was significantly negatively correlated with both Pn and Tr but more strongly with Tr, so the leaf instantaneous water use efficiency (WUEi) correlated positively with stomatal density. In conclusion, drought led to a significant increase in stomatal density and a reduction in stomatal size and aperture, resulting in decreased Pn and Tr. Because the negative correlation of stomatal density to Tr was stronger than that to Pn, leaf WUEi tended to increase.
A newly discovered silicified brachiopod interval from the Upper Member of the Guanling Formation (Late Anisian, Middle Triassic) in Guizhou Province (South China) is described for the first time. The most remarkable feature of this brachiopod assemblage, besides the very good preservation, is the very low taxonomic evenness and diversity. This impoverished, low diversity/high density assemblage is represented by more than 700 recovered specimens belonging to three species within two spiriferinid genera (Pseudospiriferina multicostata, P. pinguis, and Punctospirella fragilis). It is characterized by the overwhelming abundance of an endemic spiriferinid species, P. multicostata, which contributes to more than 90% of the community. Silicified valves of P. multicostata and Punctospirella fragilis allow detailed descriptions of the internal morphology based on direct observation. Brachiopod paleoecology, assessed by considering host−rock lithology, shell disarticulation, and shell size suggests that this endemic brachiopod fauna represents a favourable niche for development of dense brachiopod−dominated communities, i.e., high energy, hard substrate, nutrient rich environment.
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