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C-repeat binding factor gene family identified in non-heading Chinese cabbage is functional in abiotic and biotic stress response but different from that in Arabidopsis

100%

Wang Z., Wang F., Tang J., Huang Z., Xiong A., Hou X.

Acta Physiologiae Plantarum

2014

tom 36

nr 12

C-repeat binding factor (CBF) signaling pathway is involved in cold acclimation responsive to low temperature and some other stresses. CBF transcription factor family is the key component of this pathway. In this study, eight CBF-like genes, BrCBF1, BrCBF2, BrCBF3, BrCBF4, BrCBF5, and BrCBF6A/B/C were isolated from non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino, NHCC). The deduced CBF proteins shared high similarity with their Arabidopsis orthologs and localized to the nucleus. Furthermore, quantitative realtime PCR (qPCR) analysis showed that BrCBF1~3 were induced by cold (4 C) but not drought or abscisic acid (ABA), indicating that they are involved in an ABA-independent pathway; however, BrCBF4~6 were regulated by both drought and ABA, suggesting that they were involved in an ABA-dependent pathway. Nevertheless, unlike Arabidopsis, BrCBF4~6 showed response to both cold and ABA, indicates ABA-independent and ABA-dependent parts of CBF pathway in NHCC might not be completely separate, and these genes may act as the connection points in the network. BrCBFs were also accumulated in response to salicylic acid (SA), methyljasmonate (MeJA), and ethylene (ET), indicating that BrCBF genes might participate in the response to biotic stresses. Taken together, eight CBF genes were isolated from NHCC which compose a functional CBF signaling pathway by participating in response to multiple stresses and performing roles from Arabidopsis to some extent.

Cloning and characterization of the nitrate transporter gene BraNRT2.1 in non-heading Chinese cabbage

100%

Liu T., Dai W., Sun F., Yang X., Xiong A., Hou X.

Acta Physiologiae Plantarum

2014

tom 36

nr 04

To improve the efficiency of nitrogen use and to reduce the accumulation of nitrates in vegetables, an improved understanding of the mechanisms that regulate nitrate uptake and signaling is essential. Nitrogen use is regulated largely by the nitrate transporter genes, but few studies have examined the nitrate transporter genes in nonheading Chinese cabbage (Brassica rapa ssp. Chinensis Makino), one of the most important leafy vegetables in East Asia. In this study, the nitrate transporter gene BraNRT2.1 was isolated from non-heading Chinese cabbage. The cDNA for this gene contains an open reading frame of 1593 base pairs and encodes a predicted protein of 530 amino acid residues. Analysis of the BraNRT2.1 showed that BraNRT2.1 was expressed mainly in the roots and that the transcription of the gene was induced following exposure to 250 μM and 25 mM nitrate. In addition, GUS staining revealed that the BraNRT2.1 promoter directed expression to the roots. The BraNRT2.1-YFP fusion protein was observed to be localized to the plasma membrane. Finally, we observed that BraNRT2.1 could restore nitrate uptake in the presence of 200 μM nitrate in Arabidopsis thaliana plants lacking AtNRT2.1 function. Together, these results demonstrate that BraNRT2.1 encodes a high-affinity nitrate transporter that participates in nitrate uptake. These findings provide a foundation for future studies and plant breeding to improve the efficiency of nitrogen use and to reduce the accumulation of nitrates in vegetables.

Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function

84%

Li J., Xiong T., Xiao R., Xiong A., Chen J., Altaf E., Zheng Y., Zhu G., He Y., Tan J.

Archivum Immunologiae et Therapiae Experimentalis

2013

tom 61

nr 3

Ograniczanie wyników

2 Acta Physiologiae Plantarum

1 Archivum Immunologiae et Therapiae Experimentalis

3 Xiong A.

2 Hou X.

1 Altaf E.

1 Chen J.

1 Dai W.

1 He Y.

1 Huang Z.

1 Li J.

1 Liu T.

1 Sun F.

1 Tan J.

1 Tang J.

1 Wang F.

1 Wang Z.

1 Xiao R.

1 Xiong T.

1 Yang X.

1 Zheng Y.

1 Zhu G.

2 2014

1 2013

Wyniki wyszukiwania

C-repeat binding factor gene family identified in non-heading Chinese cabbage is functional in abiotic and biotic stress response but different from that in Arabidopsis

Cloning and characterization of the nitrate transporter gene BraNRT2.1 in non-heading Chinese cabbage

Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function