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This model optimizes port hinterland intermodal refrigerated container flows , considering both cost and quality degradation, which is distinctive from the previous literature content in a way that it quantifies the influence of carbon dioxide (CO2) emission in different setting temperature on intermodal network planning. The primary contribution of this paper is that the model is beneficial not only to shippers and customers for the novel service design, but also offer , for policy-makers of the government, insights to develop inland transport infrastructures in consideration of intermodal transportation. The majority of models of multimodal system have been established with an objective of cost minimization for normal commodities. As the food quality is possible to be influenced by varying duration time required for the storage and transportation, and transportation accompanied with refrigeration producing more CO2 emission, this paper aims to address cost minimization and quality degradation minimization within the constraint of CO2 footprint. To achieve this aim, we put the quality degradation model in a mixed-integer linear programming model used for intermodal network planning for cold chain. The example of Dalian Port and Yingkou Port offer insight into trade-offs between transportation temperature and transport mode considering CO2 footprint. Furthermore, the model can offer a useful reference for other regions with the demand for different imported food, which requires an uninterrupted cold chain during the transportation and storage
Algae can increase pH and dissolved oxygen via photosynthesis and affect the wastewater treatment performance of high-rate algae ponds (HRAPs). Nine laboratory-scale HRAPs divided into three groups were constructed to treat synthetic wastewater via six-month experiments. Higher algae concentrations in the influent of HRAPs effectively promoted the wastewater treatment performance. Seasonal variation had a significant impact on algal growth. Summer tests exhibited a higher reduction of pollutants than autumn tests. The high-influent algae concentration group in summer (HRAP-A) largely reduced the TN by 52.7±3.0%, TP by 90.3±1.0%, and dissolved chemical oxygen demand (DCOD) by 99.0±1.0%. It outperformed the low influent algae concentration group in summer (HRAP-B) and was significantly higher than the high influent algae concentration group in autumn (HRAP-C). The appropriate operation and design of HRAPs contributed to efficient wastewater treatments.
Actin is a globular multi-functional protein that forms microfilaments, and participates in many important cellular processes. Previous study found that Haemonchus contortus actin could be recognized by the serum of goats infected with the homology parasite. This indicated that H. contortus actin could be a potential candidate for vaccine. In this study, DNA vaccine encoding H. contortus actin was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Actin group were vaccinated with the DNA vaccine on day 0 and 14, and challenged with 5000 infective H. contortus third stage larval (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results showed that DNA vaccine were transcribed at local injection sites and expressed in vivo post immunizations respectively. For goats in Actin vaccinated group, higher levels of serum IgG, serum IgA and mucosal IgA were produced, the percentages of CD4+ T lymphocytes, CD8+ T lymphocytes and B lymphocytes and the concentrations of TGF-β were increased significantly (P<0.05). Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Actin group were reduced by 34.4% and 33.1%, respectively. This study suggest that recombinant H. contortus Actin DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.
The potential of two Cd-hyperaccumulators, Solarium nigrum L. (SN) and Beta vulgaris L. var. cicla L. (BV), as phytoremediation plants to remove Cadmium (Cd) from contaminated water was evaluated in hydroponics. The results showed that Cd exposure induced chlorosis and inhibited biomass growth in SN. However, these symptoms did not appear in BV. In BV, the Cd concentrations increased from 14.2 to 314.7 mg kg⁻¹ in the shoots and 241.1 to 4547.9 mg kg⁻¹ in the roots when the Cd concentration in the nutrient solution increased from 0.5 to 50 µМ. The corresponding increase in SN was from 7.4 to 100.6 mg kg⁻¹ in the shoots and 30.2 to 2010.7 mg kg⁻¹ in the roots. Generally, ВV showed a higher tolerance and accumulation of Cd, while SN was more efficient for Cd removal due to its higher biomass.
Populus euphratica Oliv., a species of the model woody plant genus Populus, is well known for its tolerance to salinity stress, the underlying mechanism of which is a research hotspot. Transient expression of fluorescent fusion proteins is commonly used for rapid assessment of gene functions and interactions, and thus would be useful to study the genes involved in salt tolerance in this species. Our transient gene expression protocol for P. euphratica included a simple protoplast preparation and transformation procedure from suspension cultured cells. The highest protoplast yield (8 9 107 g-1 fresh weight) with high viability (above 90 %) was obtained using an optimized enzyme mix of 4 % (w/v) cellulase R10, 0.5 % (w/v) pectinase, and 0.2 % (w/v) hemicellulase. Factors affecting protoplast transformation efficiency were also optimized: 20 lg plasmid DNA versus 105 protoplasts, and a transformation time of 20 min using PEG, which resulted in a transformation efficiency greater than 50 %. A pair of known markers was simultaneously and correctly expressed in the same P. euphratica protoplasts by co-transformation. The isolation and transformation protocol took 5 h, and results could be obtained within 24 h. This protoplast transient expression system is suitable for studying gene expression, protein localization, and protein–protein interactions in woody plants. In addition, it would be particularly useful for studying the signaling pathway involved in the salt tolerance of P. euphratica in a homologous system, which may not even be possible using protoplasts prepared from other species.
The objective of this study was to test whether biochar amendment could offset the effects of nitrogen (N) deposition on soil microbial biomass carbon (MBC) and enzyme activity. We applied N (low and high rates) and biochar (low and high rates) individually, and in combination (all permutations) to the soil of a Torreya grandis cv. “Merrillii” orchard as a 13-month field experiment during 2015-2016 in Zhejiang Province, China. MBC significantly increased in the low N treatment but decreased in the high N treatment (P<0.05). MBC significantly decreased in both biochar-only treatments, and this effect became stronger as biochar amendment rates increased. Biochar amendment amplified the positive effects of low N treatment on MBC and mitigated the negative effects of high N treatment. Catalase, cellulase, and urease activities significantly increased with N addition. Cellulase, nitrite reductase, and urease activities significantly increased in the biochar treatments. The positive effects of low N addition on catalase were amplified in the low biochar amendment treatment, but the positive effects on cellulase decreased significantly. The effects of biochar amendment on MBC and enzyme activities of soil that receives atmospheric N deposition were regulated by the rates of both biochar amendment and N deposition, and varied with enzyme type.
Algae growth and photosynthesis can be inhibited in high-rate algae ponds (HRAP) in cold climates. This study evaluated the feasibility and importance of polyester fiber strips (PFS) that can be applied to HRAP as growing sites for algae survival, thus enhancing HRAP performance. The results indicated that 42.0±2.0% TN, 86.0±1.0% TP and 99.0±1.0% DCOD were reduced in HRAP with high PFS amounts, which outperformed HRAP with low PFS amounts and was significantly higher than control. The positive role of PFS on algae biomass production could effectively remove organics and nutrient from wastewater.
The objective of this study was to determine and compare the effect of five different pretreatments including sulfite treatment (ST), osmotic dehydration (OD), steam blanching (SB), s team blanching plus osmotic dehydration (SB+OD), and ultrasound treatment (UT), on the microwave vacuum drying (MVD) kinetics, the physicochemical properties, and sensory quality of the apple slices. The results showed that the p retreatments prior to MVD could reduce the drying time of apple slices by 25–45% as compared to the non-pretreated apple slices, and the drying time in the SB+OD apple slices sample was shortest. Whether pretreated or not, MVD process was controlled by diffusion and characterized by a two-stage falling-rate drying. As calculated according to the Fick’s law of diffusion, the moisture diffusivities (Deff) ranged from 1.64×10¯⁸ to 3.46×10¯⁸ m2 /s. Different pretreatment methods had a signifi cant infl uence on the physicochemical properties and sensory qualities of the dried products (p<0.05). The SB+OD-pretreated apple slices showed the lowest shrinkage (59.5%) and the highest total sugar content (77.90 g/100 g dry matter). Besides, the OD-pretreated apple slices showed the highest density (0.953 g/cm3 ) and the lowest titratable acidity (1.67 g/100 g dry matter). In addition, the ST-pretreated samples showed the highest titratable acidity (3.21 g/100 g dry matter) and vitamin C content (12.74 mg/100 g dry matter), while the sample pretreated by SB showed the highest total phenolics content (18.37 mg/g dry matter). Non-sulfi te-pretreated samples were superior to the sulfi te treatment or the control in flavor but inferior to the sulfi te treatment in color. Results of preference sensory evaluation showed that the dried apple slices were preferred by the panelists in the following order: SB+OD>SB>UT>OD>ST>the control.
Diurnal changes in the structure of the ciliate community in surface waters were studied in the aquaculture area of Dapeng’ao cove, China. Two periods of heavy rainfall occurred during the study period, intensifying water column stratification and influencing the water’s properties. A total of 21 ciliate taxa from 15 genera were identified; the dominant species was Mesodinium rubrum. The maximum abundance of M. rubrum reached 3.92×104 indiv. dm−3, contributing 95.1% (mean value) to the total ciliate abundance. Diurnal changes in M. rubrum abundance were highly variable, the driving force probably being irradiance and food availability. The results suggest that M. rubrum may form blooms in aquaculture areas when there is a suitable physical regime with enriched nutrients, which is potentially harmful to the fish-farming industry.
It is known that the dominance of graminoid species is promoted by N addition; however, there has been relatively little effort to examine the pattern induced by natural N fertility. Since nutrie nt use e fficiency (NUE) is an important trait determining plant competitive ability, we expected that the species guild with higher NUE (lower nutrient content) may be more competitive on infertile soils. We explo red t he relationships between relative forbs biomass share , soil N and productivity by the linear regressi on analysis on a natural alpine meadow in northeast of Qinghai- Tibetan Plateau (3600 m a.s.l.). To test the variety of leaf N:P stoichiometry, paired t test and general linear model multivariate (GLM) analysis were also used. We f ound that the leaf N:P ratios of the whole community were below 13 in studied sites, which may be consistent with the N limitation on the veget ation. Graminoids re tained lower concentrations of leaf N and P than forbs in community on the Nlimited grassland. Consistent with our prediction, we found that the biomass fraction of graminoids declined with soil N content and aboveground production on the grassland. Different from the pattern along fertility gradients induced by N fertilization, our results showed that gr aminoids with lower internal nutrient content w ere able to resist low levels of nutrient availability on the natural alpine grassland when compared to forbs.
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