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Effect of glucose and deoxyglucose on cytoplasmic concentration of free Ca2 in Ehrlich ascites tumour: studies on single cells

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Czyz A., Teplova V. V., Sabala P., Czarny M., Evtodienko Y. V., Wojtczak L.

Acta Biochimica Polonica

1993

tom 40

nr 4

Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells loaded with fura-2 was measured in single cells applying a video imaging system. In resting cells [Ca2+li amounted to 60 - 340 nM and was increased after addition of 10 mM D-glucose or D-2-deoxyglucose by 80 - 200 nM. This increase occurred within 30 - 60 s following addition of the sugars and lasted for several minutes. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose and, vice versa, thapsigargin added after the sugars mobilized less Ca2+ than when added before. A possible relation of the [Ca2+]i rise evoked by glucose and deoxyglucose to the Crabtree effect is discussed.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Czarny M.

1 Czyz A.

1 Evtodienko Y. V.

1 Sabala P.

1 Teplova V. V.

1 Wojtczak L.

1 1993

Wyniki wyszukiwania

Effect of glucose and deoxyglucose on cytoplasmic concentration of free Ca2 in Ehrlich ascites tumour: studies on single cells