Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 18

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The paper was aimed at synthetic presentation of results of own studies into the occurrence and function of Aeromonas hydrophila bacteria in aquatic environments with different degrees of contamination. This species belongs to the class of gamma proteobacteria, the order of Aeromonadales and the family of Aeromonadaceae. Results of own long-term studies confirm the thesis on the ubiquity of A. hydrophila bacteria, whose occurrence reflects the trophy and contamination degree of aquatic environments, especially. Despite the relatively low numbers of these microorganisms, in environmental studies their percentage in the total bacteria count reached up to 30% in water samples and even up to 41.5% in fish samples. Taking all this into account, it seems justified to consider A. hydrophila bacteria as a potential indicator in the anthropogenic contamination of waters.
The roots of winter wheat plants, cv. Mikon, grown in 45-year monoculture, were analysed in the study. Twenty-two bacterial isolates obtained from the rhizosphere, rhizoplane, and endorhizosphere that were capable of growth at 8°C and at 28°C, were selected for further analysis. The isolated psychrotrophs accounted for 25% of all bacteria present in the wheat rhizosphere and capable of growth at 8°C. Psychrotrophic bacteria were analysed at a temperature of 10°C and 28°C to determine their ability to inhibit the growth of pathogenic fungi, solubilise mineral phosphates, and to determine their ability to degrade chitin and cellulose. Similarity between the isolates was determined by Enterobacterial Repetitive Intergenic Consensus – Polymerase Chain Reaction (ERIC-PCR) and Random Amplification of Polymorphic DNA – Polymerase Chain Reaction (RAPD-PCR). The majority of isolated psychrotrophs inhibited the growth of pathogenic fungi and solubilised mineral phosphates at both incubation temperatures. Psychrotrophic bacteria exerted a two-fold stronger inhibitory effect on mycelial growth at 10°C than at 28°C. The growth of Fusarium culmorum and F. oxysporum was inhibited to the highest extent at 10°C and at 28°C, respectively. Phosphate solubilisation rates were higher at 28°C, particularly in the rhizosphere. Regardless of temperature, the bacteria exhibited low chitin-degrading potential, and none of the isolates was capable of degrading cellulose. A high similarity between the selected psychrotrophs was revealed by ERIC-PCR and RAPD- -PCR analyses. Based on RAPD-PCR, the analysed population was divided into a group of isolates obtained from the rhizosphere, and two groups comprising representatives of both the rhizoplane and the endorhizosphere. Due to their ability to grow over a wide temperature range and increase phosphorus availability to plants, and their antagonism against pathogens, psychrotrophic bacteria can be used to improve the growth and yield of cereal crops.
The aim of this study was to define the influence of microbiological activity on the safety (microflora composition, biogenic amine amount) and colour of “kumpia wieprzowa” during the 3-month ripening period. The study included the amount of aerobic bacteria, yeast, lactobacilli rods, coagulase- negative cocci, pH and colour parameters as well as the content of nitrates (V) and (III), biogenic amines and amino acids. The lactobacilli and cocci constituted the predominant microflora of the ready-to-eat product (4.9-5.2 and 5.2-5.4 log cfu/g, respectively), although further mesophilic bacteria identification revealed the presence of numerous aerobic, aerotolerant and anaerobic species, mostly gram-positive, spore- and non-spore-forming. The absence of 2-phenylethylamine and putrescine and the low level of tryptamine (2.5 mg/kg) at the beginning of the ripening as well as the increase of tyramine and spermine amounts from 11.5 and 2.7 to 21.9 and 4.0 mg/kg, respectively during the treatment, denoted the good quality of raw meat used and dynamic growth of the desired acidifying and denitrifying microorganisms. The development of the coagulase-negative cocci population corresponded with the a* and C* colour parameters and the nitrate (III) content increase, the final result of which was 26.9, 27.5 as well as 19.4 mg/kg. The content of nitrates (V) and (III) was optimal to obtain a non-cured, safe and suitably coloured, long-term ripened meat product.
The obligatory human pathogen, Mycobacterium tuberculosis, is the most important etiological factor of tuberculosis. Unfortunately, there is little information about genetic diversity of this pathogen. The main aim of this research was the estimation of genetic diversity of M. tuberculosis on the basis of various categories of DNA markers. The genome of 32 strains were scanned by DNA markers such RAPD, IS6110 and catalase-peroxidase katG gene. All 162 identified loci were polymorphic. The genetic diversity coefficient (HT) of M. tuberculosis was 0.32 for RAPD and 0.27 for IS6110. There were 14 alleles in katG gene. All strains were characterised by the individual molecular pattern. Genetic similarity varied from 0.13 to 0.94 (RAPD markers) and from 0 to 1 for (IS6110). M. tuberculosis strains did not represent a clonal structure, single source of transmission and epidemiological relationships as well. The applied DNA markers proved to be highly efficient for analysis of genetic structure of M. tuberculosis.
The proposed research outlines a serious common concern of Salmonella resistance to antimicrobials following prolonged exposure to the disinfectants (biocides). These phenotypes of bacteria could potentially result in hard to treat infections. Typical for avian sources, biocide sensitive S. enterica subsp. enterica serovars: Typhimurium, Enteritidis, Virchow and Zanzibar and their isogenic biocide-tolerant variants were studied in order to investigate bacteriostatic effect of two commercially available biocide formulations: potassium peroxymonosulfate (P) and dodecylamine based structure (triamine, D). We found that cultivating of the bacteria in the medium supplemented with a blend containing P did not influence their antibiotic susceptibility pattern. In contrast, tolerance of bacteria to D compound resulted in resistance to co-trimoxazole, cefotaxime and ciprofloxacin of which two cefotaxime and ciprofloxacin are used commonly for the treatment of invasive Salmonella infections in humans. The dependency between OMP patterns and the level of Salmonella survival in media containing the biocides was observed merely in serovar Typhimurium. In conclusion, these results suggest that Salmonella strains challenged by prolonged treatment with the disinfectants become resistant to antibiotics, however it depends on Salmonella serovar and the chemical used. This paper also highlights the loop-mediated isothermal amplification (LAMP) as a technique that offers great benefits to microbiological detecting of Salmonella species in the samples.
In 2004-05 counts of the bacteria proteolytic, ammonifying, AOB and NOB, NO₃-N to NO₂-N reducing, denitrifying, and atmospheric nitrogen fixing (Azotobacter sp. and Clostridium pasteurianum) were analyzed along with temperature, pH, dissolved oxygen saturation, ammonia, nitrite and nitrate nitrogen in waters of the River Drwęca. The counts of the above groups of bacteria ranged within a few orders of magnitude depending on the physiological group and sampling site. The smallest were the counts of autotrophic nitrifying and atmospheric nitrogen fixing bacteria Azotobacter sp. (10² cfu·1 cm⁻³) and Clostridium pasteurianum (10² MPN·100 cm⁻³). The most numerous were ammonifying bacteria (10³ – 10⁶ MPN·100 cm⁻³). The quantitative occurrence of the bacteria in question varied in relation to the physical and chemical parameters of the water, which was evidenced by Spearman’s statistical analysis.
Water in the Drwęca River has been monitored in terms of differences in counts of bacteria which are active in transformations of nitrogen compounds depend on various man-made activity. In 2000–2001 samples of waters were assayed for counts of proteolytic, ammonifying, AOB, NOB, N-NO₃ to N-NO₂ reducing bacteria, denitrifying bacteria as well as anaerobic (Clostridium pasteurianum) and aerobic (Azotobacter sp.) atmospheric nitrogen binding bacteria, which differed within several orders (0–10⁵ cfu 1 cm⁻³ or MPN 100 cm⁻³) depending on the analysed physiological group of bacteria, sampling site or date. Statistically significant differences in counts of particular groups of microorganisms in the analysed water samples collected from the Drwęca River proved between the sampling dates only confirm that the river may be seasonally polluted by domestic sewage from towns and villages located in the river catchment or be associated with the cyclic nature of fisheries and/or agricultural production.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.