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The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
The objective of this study was to determine the stimulating effect of the brewers' yeast Saccharomyces cerevisiae dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in lambs. The study involved 32 lambs aged 30 ±3 d, divided into two equal groups: control and experimental. Animals in the experimental group were fed a C-J concentrate mixed with a prebiotic, the extract of dried brewers' yeast containing 10%-15% MOS and 25%-30% ß-l,3/l,6-D-glucan in the amount of 3 g/kg of the concentrate. At the beginning of the experiment (day 0) and on the 15th, 30th, and 60th d of the study, blood was sampled from the jugular vein to determine γ-globulin levels, lysozyme and ceruloplasmin activity, proliferative response of blood lymphocytes (MTT) after stimulation with LPS or ConA, metabolic activity (RBA), and potential killing activity (PKA) of phagocytes. As regards humoral immunity parameters, significantly higher γ-globulin levels and higher lysozyme and ceruloplasmin activity were noted in the blood serum of experimental lambs supplemented with the yeast extract, in comparison with control lambs not fed the supplement. No statistically significant differences in serum total protein were found between the control and experimental groups. The analysis of cellular immunity indicators revealed significantly higher levels of RBA and PKA, and higher MTT rates after stimulation with LPS or ConA in the experimental group, in comparison with the control group.
The objective of this study was to determine the effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs. The experimental material comprised 32 lambs aged 30 ± 3 days, divided into two equal groups: control and experimental. Experimental group animals were fed a diet supplemented with the Biolex-MB40 (Saccharomyces cerevisiae) in the amount of 3 g/kg of the concentrate. At the beginning of the experiment (day 0) and on experimental days 15, 30 and 60, blood was sampled from the jugular vein to determine and compare the phagocytic activity (PHAGOTEST) and oxidative metabolism (BURSTTEST) of peripheral blood granulocytes and monocytes by flow cytometry. Based on the results of an analysis of granulocyte and monocyte phagocytic activity, statistically higher levels of phagocytic activity were observed in the group of lambs administered Biolex-MB40 than in the control animals, expressed in terms of the percentage of phagocytic cells as well as mean fluorescence intensity. Biolex-MB40 also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism as well as mean fluorescence intensity.
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