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1

Protective effects of hydrogen on oxidative stress and liver function during CO2 pneumoperitoneum in dogs

100%
Zhang J., Zheng X., Jiang L., Tao Z., Liu T.
Medycyna Weterynaryjna
|
2020
|
tom 76
|
nr 10
The purpose of this study was to investigate the protective effects of hydrogen reducing ischemia-reperfusion injury during CO2 pneumoperitoneum on oxidative stress and liver function. Eighteen healthy Beagle dogs were divided into three groups. Dogs in the control group were subjected only to anesthesia for 90 min. The pneumoperitoneum group was subjected to the pressure of CO2 pneumoperitoneum with 12 mmHg intraabdominal pressure for 90 min. The hydrogen group was subjected to the pressure of CO2 pneumoperitoneum with 12 mmHg intra-abdominal pressure for 90 min after a subcutaneous injection of hydrogen gas (0.2 mL/kg) for 10 min. Blood samples were collected before the induction of pneumoperitoneum, as well as 2 h and 6 h after deflation, to evaluate oxidative stress and liver function in serum. Liver tissue samples were taken 6 h after deflation for histopathological examination. In comparison with group P, a milder histopathological change was found in group H2, and the levels of hepatic function and anti-oxidation in group H2 were higher. Hydrogen gas reduced liver ischemia-reperfusion injury due to CO2 pneumoperitoneum by reducing oxidative stress and improving liver function. Hydrogen gas therapy can be considered as a way to reduce liver ischemiareperfusion injury in laparoscopic surgery.
2

The Wnt7b/β-catenin signaling pathway is involved in the protective action of calcitonin gene-related peptide on hyperoxia-induced lung injury in premature rats

100%
Wang S., Dang H., Xu F., Deng J., Zheng X.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Background: Calcitonin gene-related peptide (CGRP) can protect against hyperoxia-induced lung injury, making the upregulation of CGRP a potential therapeutic approach for this type of injury. However, the effects of CGRP on the Wnt7b/β-catenin signaling pathway are unclear. In this study, we investigated the roles of CGRP and the Wnt7b/β-catenin signaling pathway in hyperoxia-induced lung injury. Methods: Premature Sprague Dawley (SD) rats were exposed to 21, 40, 60 and 95% oxygen for 3, 7 and 14 days. The animals’ body weights, survival rates and endogenous CGRP levels were measured. Lung samples were harvested for histological analyses and measurements of malondialdehyde (MDA) concentration and total antioxidant capacity (TAOC). We also assessed the MDA concentration and TAOC in the lung tissues after administration of 200 nmol/kg CGRP8–37 (a CGRP antagonist). Finally, alveolar epithelial type II (AEC II) cells were isolated from premature rats, exposed to 21 or 95% oxygen for 3, 7 and 14 days, and treated with 10− 8 mol/l exogenous CGRP. The protein expressions of Wnt7b and β-catenin were assessed using western blotting, and TCF and c-myc mRNA expressions were assessed using qPCR. Results: Rats exposed to 60 and 95% oxygen had significantly lower body weights and survival rates than the 21 and 40% groups, and the decrease was time dependent. Endogenous CGRP was elevated in the lung tissues of premature rats exposed to 95% oxygen. CGRP8–37 induced apparent inflammation in the lung tissue and alveolar structural remodeling. In addition, the expression levels of Wnt7b and β-catenin were markedly increased after exposure for 3 days. They peaked at 7 days, then declined at 14 days. The levels of TCF/c-myc in AEC II cells increased significantly after CGRP treatment when compared with cells that had only undergone hyperoxia. Conclusions: CGRP protected against hyperoxia-induced lung injury in premature rats. This process involves the Wnt7b/β-catenin signaling pathway.
3

Quantitative research on the relationship between salinity and crack length of soda saline-alkali soil

100%
Ren J., Li X., Zhao K., Zheng X., Jiang T.
Polish Journal of Environmental Studies
|
2019
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tom 28
|
nr 2
The Songnen Plain in China has a typical type of soda saline-alkali soil that frequently shrinks and cracks under natural conditions, especially during water evaporation. This study aims to study the effects of salt contents on the cracking process of soda saline-alkali soils and to make a quantitative analysis between crack length and some primary salt parameters of soda saline-alkali soils. To achieve the objectives, 57 soil samples with different salinity levels were selected and a laboratory-controlled desiccation cracking test was then conducted. Next, correlation analysis was carried out between extracted crack lengths and salt parameters of all the specimens. The results showed that there are clear linear dependencies between crack length and salinity, Na⁺, Cl⁻, and electrical conductivity (EC), and that CO₃²⁻ is also quite linear with crack length. The fitting results conducted from the verification experiment showed that Na⁺ content, Cl⁻ content, EC, and total salinity showed high prediction performance (R²>0.91 and RPD>2.5); that the CO₃²⁻ content had quite reasonable accuracy (R²= 0.78 and RPD = 1.82) but with less stability and poor reliability; that the prediction accuracy for pH, however, was very poor, with R² and RPD only 0.42 and 1.06, respectively.
4

The possible role of BAX and BI-1 genes in chilling-induced cell death in cucumber fruit

100%
Zhao Y., Chen J., Tao X., Zheng X., Mao L.
Acta Physiologiae Plantarum
|
2014
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tom 36
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nr 06
The aim of the present study was to investigate the possible role of BAX and BI-1 genes in chilling injury of cucumber fruit. BAX and BI-1 gene expressions were assayed under 2 ± 1°C. Meanwhile, cell death, cellular integrity, specific chromatin fragmentation and nucleus morphology in cucumber (Cucumis sativus L. cv. Zhexiu1) fruits were determined. Results indicated that BAX and BI-1 genes were activated by low temperature and the expression level of the BAX was much higher than BI-1. At the same time, electrolyte leakage and cell death were increased coupled with nuclear envelope disassembly and DNA fragmentation during the occurrence of chilling injury. In addition, characteristic features of programmed cell death were induced as well as the initiation of chilling injury. The interaction of BAX and BI-1 might predetermine the cell life or death in response to cold stimulus.
5
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The cross-sectional survey on COVID-19 vaccine hesitancy and it predictors among Chinese parents of 3-17 years aged children in Shenzhen City

100%
Li T., Qiu X., Gong X., Zhan R., Zheng X.
Annals of Agricultural and Environmental Medicine
|
2022
|
tom 29
|
nr 1
6
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Mental health status of frontline clinical nurses fighting the Omicron variant of COVID-19 - a cross-sectional survey in China

100%
Li T., Qiu X., Gong X., Zhan R., Zheng X.
Annals of Agricultural and Environmental Medicine
|
2022
|
tom 29
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nr 3
7
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Content available

CO2 concentration affects in vitro pig embryo developmental capacity

86%
Zhang L., Lin Z., Bi Y., Zheng X., Xiao H., Hua Z.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 3
8

Genetic structure among four populations of paddlefish, Polyodon spathula (Actinopterygii: Acipenseriformes: Polyodontidae), based on disomic microsatellite markers

86%
Zheng X., Schneider K., Lowe J. D., Gomelsky B., Mims S. D., Bu S.
Acta Ichthyologica et Piscatoria
|
2014
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tom 44
|
nr 3
Background. The paddlefish, Polyodon spathula (Walbaum, 1792), is an important species for commercial and recreational fisheries throughout the central United States. Populations have declined in many areas due to river modification, loss of spawning habitat, pollution, and over-exploitation. Assessing genetic diversity of a species is an important consideration for developing conservation plans. The goal of this research was to perform a broad range survey of paddlefish diversity by evaluating populations from geographically distant major rivers of the United States of America. Materials and Methods. Paddlefish samples were collected from four sites including the Alabama River, Red River, Yellowstone/Missouri River, and Ohio River. Eight microsatellite loci (PspD102, PspD111, PspB105, PspD9, PspD8, PspC6, PspH26, and PspC10) that displayed disomic inheritance patterns were used for the amplification of alleles. Results. Average allelic richness of four sites ranged from 7.50 ± 1.36 to 5.46 ± 0.91. Average expected heterozygosity ranged from 0.717 ± 0.085 to 0.591 ± 0.093, the average observed heterozygosity assumed the values from 0.711 ± 0.115 to 0.585 ± 0.087. A moderate level of between population diversity was observed with an overall Fst value of 0.0702. Hardy–Weinberg equilibrium revealed that seven loci in the four populations were in equilibrium. The four populations were clustered to two categories by cluster analysis (UPGMA) based on Fst and δμ2 distance. Conclusion. Four studied paddlefish populations exhibited relatively low levels of genetic diversity and close relative relations, but still had some differentiation among the populations. The genetic distance and Fst revealed that the Ohio River, Red River and Yellowstone/Missouri River populations belong to the same branch, while the Alabama River population from another branch.
9

Nonlinear PTO effect on performance of vertical axisymmetric wave energy converter using semi-analytical method

86%
Liu M., Liu H., Zheng X., Chen H., Wang L., Zhang L.
Polish Maritime Research
|
2017
|
tom 24
|
nr Special Issue S3
The wave energy, as a clean and non-pollution renewable energy sources, has become a hot research topic at home and abroad and is likely to become a new industry in the future. In this article, to effectively extract and maximize the energy from ocean waves, a vertical axisymmetric wave energy converter (WEC) was presented according to investigating of the advantages and disadvantages of the current WEC. The linear and quadratic equations in frequency-domain for the reactive controlled single-point converter property under regular waves condition are proposed for an efficient power take-off (PTO). A method of damping coefficients, theoretical added mass and exciting force are calculated with the analytical method which is in use of the series expansion of eigen functions. The loads of optimal reactive and resistive, the amplitudes of corresponding oscillation, and the width ratios of energy capture are determined approximately and discussed in numerical results
10
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Content available

A new adaptation for in vitro co-culture of single porcine parthenogenetic embryos using feeder cells

72%
Zhang L., Lin Z., Hua Z., Zheng X., Xiao H., Hua W., Ren H., Zhu Z., Molenaar A., Bi Y.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 4
11
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Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig

72%
Hua Z., Xu G., Liu X., Bi Y., Xiao H., Hua W., Li L., Zhang L., Ren H., Zheng X.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 1
Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
12

Genome-wide identification of Ziziphus jujuba TCP transcription factors and their expression in response to infection with jujube witches’ broom phytoplasma

72%
Chen P., Li J., Ye X., Tan B., Zheng X., Cheng J., Wang W., Wang H., Gu L., Feng J.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 06
Phytoplasmas are plant pathogenic bacteria that inhabit the phloem sieve elements in infected plants and lack a cell wall. Infection of the woody perennial Ziziphus jujuba with phytoplasma causes jujube witches’ broom (JWB), a disease that causes heavy crop losses. The TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) are plant-specific regulators of plant morphogenesis, development, and defense after phytoplasma infection. While the TCP gene family has been studied in many plants, there is no report on TCPs in jujube. In this study, 21 ZjTCP TFs were identified and bioinformatically analyzed based on the genome of the Dongzao cultivar. Another 2 ZjTCPs were found in the genome of the Junzao cultivar. Physicochemical properties of the ZjTCP proteins were quite varied, indicating possible versatility of functions. ZjTCP proteins ranged from 172 to 590 amino acids (aa), had isoelectric points (pIs) between 5.53 (ZjTCP1) and 9.81 (ZjTCP11), and molecular weights ranging from 19,279.98 (ZjTCP11) to 61,784.17 kDa (ZjTCP19). Interaction network mapping predicted several hubs, such as ZjTCP6, ZjTCP7, ZjTCP8, ZjTCP15, ZjTCP16, and ZjTCP19, among which ZjTCP6 and ZjTCP16 were predicted to function in plant defense and morphogenesis. Spatiotemporal expression analysis of the ZjTCPs showed that ten of the ZjTCP genes were detected after infection with ‘Candidatus Phytoplasma ziziphi’. ZjTCP6, ZjTCP7, ZjTCP16, and ZjTCP19 were up-regulated after phytoplasma infection. ZjTCP16 showed the most significant increase in transcript levels, after the emergence of disease symptoms. ZjTCP12, ZjTCP15, and ZjTCP18 were down-regulated after phytoplasma infection. We concluded that ZjTCP6 and ZjTCP16 were most likely regulatory factors with roles during the plant response to jujube witches’ broom.
13

Screening of SSR markers associated with scale cover pattern and mapped to a genetic linkage map of common carp (Cyprinus carpio L.)

72%
Xiao T., Lu C., Xu Y., Li C., Zheng X., Cao D., Cheng L., Mahboob S., Sun X.
Journal of Applied Genetics
|
2015
|
tom 56
|
nr 2
14

Detection of frequently used pesticides in apple orchard soil in China by high resolution mass spectrometry

72%
Shi R., Li Y., Chen M., Zheng X., Liu X., Zhao Y., Liu A., Jia J., Xu M., Zhao Z.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 2
15

Detection of Dirofilaria immitis antigen and antibodies against Anaplasma phagocytophilum, Borrelia burgdorferi and Ehrlichia canis in dogs from ten provinces of China

59%
Wang J., Kelly P., Zhang J., Shi Z., Song C., Zheng X., Zhang Y., Hao Y., Dong H., El-Mahallawy H. S., Xiong W., Wang H., Li J., Zhang X., Wang C.
Acta Parasitologica
|
2018
|
tom 63
|
nr 2
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