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Wild raspberry (Rubus idaeus L.) is abundantly grown and widely distributed in temperate regions of lower Himalayan range which represents rich biological diversity for raspberry. Thirty accessions were explored from three different locations of Azad Jammu and Kashmir (Pakistan), several traits were visualized at sampling locations and some characteristics were evaluated and quantified after harvest of leaf and fruit samples. The accessions differed greatly in growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, fruit shape and color. Concerning quantitative characteristics, significant differences were found in plant height, leaf length and width, number of braches per plant, duration of flowering, fruit setting percentage, days to maturity, fruit length, width and weight among the samples collected from different locations. Ripe fruits were evaluated for their chemical composition i.e. moisture, carbohydrates, protein, fat, fiber, ash, TSS, acidity, pH and mineral (K, Ca, Na, Fe, Cu, Zn, Mn, Pb, and Cr) contents. The location also had significant effect on chemical composition of ripe fruits.
Contamination levels of antibiotics, namely ofl oxacin (OFL), ciprofl oxacin (CIP), levofl oxacin (LEV), oxytetracycline (OTC), and doxycycline (DOX), were quantifi ed in wastewater, soil, plants, and underground water of surrounding areas of the pharmaceutical industry in Lahore. HPLC with a DAD detector, C-18 column, and solid-phase cartridges were used to analyze antibiotic residues. In wastewater CIP was 3.0-5.25 mg/L, LEV was 0-6.20 mg/L, OFL was 2.45-4.12 mg/L, OTC was 0-9.40 mg/L, and DOX was 1.58-6.75 mg/L. From wastewater, antibiotics accumulate in environmental segments and impact human health. From wastewater, antibiotics accumulate in soil and plants and percolate to groundwater. The contamination level was 1,000 times higher in wastewater samples than other samples, as it is the primary source of antibiotics entering the environment. After wastewater, soil was the most contaminated environmental segment and groundwater was least contaminated by these residues, but water is very important as it is source of accumulation of antibiotics for human and animal bodies as well as to plants.
Sweet potato (Ipomoea batatas (L.) Lam) is an important crop due to its cultivation as staple food for millions of small farmers as well as for poor people in Latin America, Asia, Africa and in many other parts of the world. This tuberous crop is susceptible to drought stress especially during the period of crop establishment as well as vine development and tuber initiation. Yield of the crop vary widely among farmers due to improper planting systems. This current study was aimed to investigate the influence of various irrigation intervals and planting systems on vegetative growth, storage root yield and quality of sweet potato (Ipomoea batatas (L.) Lam) cv. ‘White star’ under field conditions. Three irrigation intervals (7, 14 and 21 days for summer crop, and 14, 28 and 42 days for winter crop) and two planting systems (bed planting and ridge planting) were adopted in this study. Vine length, number of branches and average leaf area significantly reduced as the irrigation interval was increased. Ridge planting produced longer vines with greater leaf area in winter crop as compared to bed planting. Yield parameters (storage root length, storage root diameter, number and fresh weight of marketable roots per plant) were directly linked with vegetative growth especially in summer crop. Under water stress conditions, as vegetative growth decreased storage root yield was also reduced. However, yield attributes were not affected by the planting systems. Vitamin C content decreased with water stress, whereas total soluble solids (TSS) and leaf proline content significantly increased with water stress in summer crop. Ridge planting also resulted in increased leaf proline content in summer crop. It is concluded that for attaining good vegetative growth and storage root yield, sweet potato should be irrigated at an interval of 7 days during summer and 14 days during winter crop and planted on ridges.
In the present work, genetic diversity in nutritional composition of sixteen plum genotypes growing at four different locations of Tehsil Rawalakot, District Poonch of Azad Jammu and Kashmir (Pakistan) were studied. Various parameters like moisture, dry matter, ash and total soluble solids contents, acidity, pH, vitamin C and sugar content, shelf-life and sensory/organoleptic evaluation, anthocyanins, phenolics and antioxidant activity were evaluated and variation in these characteristics has been discussed. The results suggested that the genotypes differed in their nutritional composition of fruits, anthocyanin and phenolic contents and antioxidant activity of fruit. The results of the present study regarding the nutritional status of existing plum germplasm will contribute and increase our knowledge about the genus Prunus and broaden the gene pool available for future plant breeding programs.
A field study was conducted to evaluate the effect of different plant growth regulators (PGRs) applied through seed priming and foliar spray on growth and yield of three okra cultivars grown under calcareous soils. The cultivars of Punjab Selection and Sabzpari produced significantly higher number of branches and leaves per plant, pod length and diameter and pod yield as compared to cv. Green ferry, while, cv. Punjab selection produced significantly greater number of flowers and pods per plant as compared to other two cultivars. Seed germination (%), plant height, and fruit set (%) were not affected by the cultivars. Among the PGR treatments, seed primed with GA3 resulted in significantly higher germination percentage and greater plant height at flowering. Seed priming and foliar spray with NAA and GA3 were effective in increasing the final plant height, number of branches, number of leaves, number of flowers and number of pods per plant and fruit set (%), fresh weight per pod and pod yield. However, pod diameter, pod moisture content and dry weight per pod were not influenced by the PGR treatments applied. These results suggested that the PGRs have great potential to improve seed germination, enhance growth and increase yield of okra cultivars under calcareous soils.
Different physiological behavior of a wide range of varieties and species belonging to the Citrus genus was analyzed when subjected to salt stress with the aim to seek new sources of tolerance that might be specie-specific. Our goal was to use physiological results obtained along a salt stress in order to clarify if it would be possible to associate them with the known citrus genetic diversity. For that purpose, we have selected 20 different genotypes representing the major species on the basis of the genetic diversity of Citrus genus complemented with one intergeneric hybrid Carrizo citrange (C. sinensis 9 P. trifoliata). A moderate salt stress of 75 mM of NaCl was applied for 12 weeks. For control plants, the main parameters contributing for more than 25 %to the diversity on the two axes of principal component analysis (PCA) were chlorophyll content, photosynthesis and Fv/Fm under light. However, the dispersal of species and varieties on the PCA did not show any particular structure. Under salt stress condition, four parameters (leaf chloride content, leaf chlorophyll content, photosynthesis and stomatal conductance) contributed more specifically to the dispersion on PCA representation with more than 15 % of contribution for each parameter. Large differences were observed within citrus genus: mandarin and pummelo presented good tolerance to salt stress while citron was very sensitive. Furthermore, all secondary genotypes that presented good tolerance to salt tolerance shared mandarin or pummelo as female parent.
Locally available bentonite clay has been modified by magnesium and used to eliminate acid blue 129 from aqueous solutions. The adsorption was studied under different experimental conditions such as dye concentrations, temperature, and shaking time. The adsorption of the dye increased with time and followed the pseudo-first-order kinetic with rate constant “k” 0.126 min-1 at 283 K. Thermodynamic parameters such as ΔH°, ΔS°, and ΔG° were calculated from the slope and intercept of the linear plots of ln K against1/T. Analysis of adsorption results obtained at temperatures of 283, 293, 303, and 313 K showed that the adsorption pattern on bentonite seems to follow Langmuir and Freundlih. The increase in temperature reduces adsorption capacity by magnesium-modified bentonite due to the enhancement of the desorption step in the mechanism. The activation energy of the adsorption process was found to be 3.55 kJ mol-1. The Mg-bentonite showed better adsorption than Ba and Al-bentonite. Our study reveals that abundantly available local clay may be used to eliminate dyes from aqueous solutions.
Drought is a vice to world crop production, exponentially enhanced by global climate change. Rice, a basic food crop for a major chunk of world populace, is largely affected by environmental challenges such as drought, salinity and heavy metal. This study brings to limelight differential drought tolerance capacity of rice varieties indigenous to North East India, a hot bed of indica rice diversity. Initial screening of rice varieties were performed through physiological dose-dependent studies under PEG (0%, 10%, and 20% which is equal to osmotic potential values of 0.001, 0.54 and 1.09 MPa, respectively), induced drought stress for three time intervals of 1, 3 and 5 days. Hierarchical clustering of the parameters on which the cultivars were analysed revealed Tampha and KMJ 1-12-3 to be relatively more tolerant whereas Chandan and Ketaki Joha as the sensitive ones. Biochemical studies for hydrogen peroxide (H₂O₂), proline, lipid peroxidation (MDA), lipoxygenase (LOX), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) were then performed on two cultivars (Tampha and Chandan) to elucidate the differential tolerance to drought, focusing on anti-oxidative response mechanism. The biochemical fingerprint showed significantly lower accumulation of H₂O₂ and MDA; higher accumulation of proline; and higher activity of SOD, CAT, DHAR, MDHAR and GR in the tolerant variety Tampha when compared to Chandan. In addition, alteration of chlorophyll fluorescence due to stress was also monitored to ascertain the variation in photosynthetic efficiency between the tolerant and sensitive cultivars. Tampha showed better photosynthetic activity in comparison to Chandan as quantified by measuring chlorophyll fluorescence. This manuscript thus throws new light into the drought stress response of the varieties from North East India with global implications.
Contaminated wastewater discharged from tanneries in Pakistan creates a lot of pollution problems. This study presents the treatment of wastewater samples collected from inlet and outlet sections of Kasur Tannery Waste Management Agency (KTWMA) in Kasur, Pakistan. The procedure utilizes hydrogen peroxide (H₂O₂) in the presence of ultraviolet (UV) radiation for the successful degradation of pollutants present in tannery water. The treatment of polluted water samples with UV/H₂O₂ for the duration of either 45 minutes or 90 minutes has greatly affected the water quality parameters such as biochemical oxygen demand (BOD), chemical oxygen demand (COD), chlorides, chromium, pH, total dissolved solids (TDS) and total suspended solids (TSS). It was concluded that the contaminants were degraded and changed to a great extent as compared to their original concentrations. This process can be considered to be a clean process as it is an environmentally friendly process and does not produce pollutants and solid wastes like other conventional processes.
The current investigation was undertaken to assess the toxic potential of synthetic insecticides (K.Othrin and bio-max) and botanical extracts (Mentha royleana L. and Artemisia absinthium L.) against Tribolium castaneum in the laboratory. Different concentrations of insecticides and botanical extracts were used following complete randomized block design. The results indicated that the toxic effects were directly proportional to concentrations of insecticide and botanical extracts. Higher concentrations had more resilient toxicity than lower concentrations. Among the tested insecticides, Biomax (Chlorpyrifos) showed >90% and K-Othrin (Deltamethrin) <80% mean toxic effect at 2% concentrations. In the botanical extract, Mentha royleana toxicity at 5% concentration is > 90% as compared to Artemisia absinthium against T. Castaneum. The results could be helpful in designing an effective management plan for the control of T. castaneum.
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