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W pracy badano adhezję i powstawanie biofilmu in vitro szczepów Pseudomonas aeruginosa do powierzchni cewników medycznych, wykonanych z silikonowanego lateksu, poliuretanu i polichlorku winylu. Zastosowano czułą i łatwą w wykonaniu metodę redukcji substratu TTC do nierozpuszczalnego czerwonego formazanu przeprowadzonej przez żywe bakterie.
The genetic features of each isolate were determined by enterobacterial repetitive intergenic consensus (ERIC) primer sequences used in PCR and by searching for six virulence genes (alg D, las B, tox A, plc H,plc N, exo S). 49 (79%) of the isolates were distributed in three ERIC PCR subgroups and showed 62% of similarity. The remaining 13 strains generated unique patterns. The first subgroup was primarily composed of isolates from faeces, these strains indicated over 70% relationship with the next subgroup, and primarily contained strains isolated from wounds and bronchial washings and the last subgroup contained strains isolated from wounds and urine. The unique strains were isolated mainly from urine. Statistical analysis indicated that variations in distribution of virulence genes in P. aeruginosa isolates with respect to strain origin and genomic subgroups were not significant. In the group of 49 strains, 100% gave a positive reaction to alg D, las B and plc H genes, 91.8% to tox A and plc N genes and 83.7% to exo S gene. Among the strains that generated unique (ERIC-PCR) patterns, 69.2% gave a positive reaction to alg D gene, 84.6% to las B gene, 76.9% to tox A, plc N and plc H genes, and 46.15% to exo S gene.
Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR analysis is a more discriminatory method than PCR ribotyping analysis and traditional serotyping scheme. We suggest that maximum discrimination can be achievied by a combination of these methods.
Evaluation of adherence of Y. enterocolitica strains isolated from humans and pigs to cheek epithelium cells obtained from humans and pigs was the aim of present study. 51 strains of Yersinia enterocolitica including 34 isolated from the faeces of people who showed typical symptoms of intestinal yersiniosis and 17 isolated from pigs were used in the study. The all Y. enterocolitica strains which were used in the investigation showed the ability of adherence to the cells of cheek epithelium from humans. The cells of Y. enterocolitica strains which had yadA gene more numerously adhered to the cells of cheek epithelium from humans than the cells of strains which did not have yadA gene.
W pracy badano hamujący wpływ dekstranu na adhezję szczepów Pseudomonas aeruginosa do komórek nabłonka policzka in vitro. Dla stwierdzenia, czy hamujące działanie dekstranu było swoiste, czy też nie, dokonano również oceny innych neutralnych polisacharydów, tj. mannanu i glikogenu.
The effect of water extracts from common buckwheat (Fagopyrum esculentum Moench) tissues on seed germination and seedling growth of winter wheat and lettuce was examined. Two kinds of extracts were evaluated: from the aboveground parts and roots of common buckwheat (cv. Hruszowska). The effect of extracts was dependent on their kind, the time of action and the species of studied plants. Lettuce seeds were more sensitive to treating with extracts from buckwheat tissues than wheat seeds. Extract from the aboveground parts of buckwheat more clearly inhibited germination of wheat seeds than extract from roots. In lettuce the reverse phenomenon occurred: two times less seeds germinated in the presence of extract from aboveground parts of buckwheat than under the influence of extract from roots. Extracts from buckwheat tissues also inhibited the growth of roots of the studied species and the coleoptiles of wheat and hypocotyls of lettuce. Extract obtained from the aboveground parts of common buckwheat showed stronger inhibiting effect.
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