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The projections from reticular formation (RF) to the pyramis (Pr; related to innervation of axial and proximal forelimb muscles) and uvula (Uv; interconnected with vestibular system) were studied using two fluorescent retrograde tracers. Bilaterally labeled RF neurons, parent for the reticulo-cerebellar projection, were found in the magno- (Lcmc) and parvocellular (Lcpc) pars of caudal lateral nucleus (n=7797), oral lateral nucleus (Lo; n=3893), and in smaller number in the lateral reticular nucleus (LR; n=1326) and gigantocellular nucleus (RGc; n=1319). The projection is seven times greater to Pr than to Uv. Connections from Lcmc to Pr originate from entire nucleus, except for the dorsolateral region, and to Uv arise from two separate neuronal populations, the ventromedial and dorsolateral, at rostral levels. While entire Lcpc supplies Pr, its rostral dorsolateral region connects Uv. The ventral LR region projects to Pr, whereas two groups of neurons in the caudal part project ipsilaterally to Uv. In Lo, neurons supplying Pr are present ventrolaterally, but these connecting Uv cluster in the dorsolateral and dorsomedial regions. Central core of the caudal RGc sends fibers exclusively to Pr. The RF projections differ regarding regions of origin and laterality, probably due to different function of Pr and Uv.
The locus coeruleus (LC) located in the pontine tegmentum, is the major noradrenergic nucleus of the brain. It gives rise to fibers innervating extensive areas within the neuraxis, among other the cerebral cortex, basal forebrain, limbic system, thalamus, brainstem autonomic nuclei, spinal cord. Throughout these projections, LC as a crucial wakefulness-promoting nucleus, is involved in neuronal circuits controlling a number of physiological functions, e.g., regulation of arousal and autonomic activity. A few data concerning the LC-cerebellar projections indicate, that LC sends same fibers to the cerebellar vermis, and small collateral projection to both the cerebral cortex and cerebellum exist as well. The aim of present study was to identify LC neurons projecting to the caudal vermal lobule, i.e. the pyramis. Following fluorescent tracer injection into the pyramis, retrogradely labelled neurons, as parent for the LC – pyramis projection, were found in defined regions of LC. The projection is bilateral with ipsilateral predominance (82% ipsiversus 18% contralateral). The LC noradrenergic connections may modulate response of the pyramis neurons and exert influence on activity of the postural muscles of upper trunk and the proximal forelimb muscles.
Pontine nuclei (PN) are large center transferring cortical afferents to the cerebellum. The study addresses to distribution of PN neurons in the pontocerebellar link. Two different neuroplasmatic and nuclear fl uorescent tracers were injected in separate experiments into: (1) paramedian lobule (PML) and pyramis, and (2) pyramis and uvula. Following both combination of injections, retrogradely labeled neurons were found bilaterally in all PN apart from the ventral nucleus. These neurons, parent for the pontocerebellar projection, clustered in two groups. First larger group occupied the dorsolateral nucleus and the dorsolateral region of lateral and peduncular nuclei. Second smaller group involved the dorsal region of paramedian nucleus and the dorsomedial region of peduncular nucleus. Projection to the uvula arises from nearly entire rostrocaudal extent of PN, and that to the pyramis and PML originates from medial and caudal extent of PN. In spite of substantial overlap of projection area in PN, no neurons were found to project by way of axonal collaterals. The strongest PN projections arise from the dorsolateral nucleus and these from the paramedian, lateral and peduncular nuclei are about two, three and fi ve times weaker, respectively. PML and the uvula receive the most numerous projections (mean 4 744 and 4 283 neurons) whereas projection to the pyramis (mean 1 172 neurons) is apparently smaller. The differences in the projection seem to be cause of different function of these lobules.
Rat medial gastrocnemius is composed of the proximal and distal compartments. To diversify these subvolumes, glycogen depletion technique based on a stimulation protocol one of the two primary nerve branches to the muscle was applied. The area of compartments, number and diameter of muscle fibers in the two distinct subvolumes on five muscle levels (10, 25, 40, 75 and 90% of muscle length) were determined. It was shown that the two smallest, opposite serial sections: close to the knee (10% of muscle length) and close to the Achilles tendon (90% of muscle length) were occupied by only one compartment, i.e. proximal and distal, respectively. In the largest section (40% of muscle length), the proximal compartment constituted 27–38% of the muscle area. Maximal number of muscle fibers in the proximal compartment was 4536–6698, while in the distal one 4773–6241. The mean muscle fibers diameter in the proximal and the distal subvolumes ranged: 36.9–54.3 µm and 46.5–63.8 µm, respectively. Additionally, in electrophysiological experiments the forces evoked by common or separate stimulation of L4 and L5 ventral roots in whole muscle and in one of compartments were measured. The ratio of forces evoked at L5/L4 ventral roots stimulation amounted to 2.18 in the proximal compartment, whereas 64.67 in the distal compartment.
The aim of the study was to determine the effect of body condition score (BCS) of cows during the last weeks of pregnancy and in the first months of lactation on the incidence of postpartum complications. A total of 528 cows were included in the study. No significant effect was found of BCS of cows ap on the incidence of dystocia, corpus luteum pseudograviditatis, ovarian cysts, repeat breeders, infertility and the service period (SP). In turn, BCS before calving had an effect on the incidence of the retention of the placenta, puerperal metritis and clinical endometritis (MET), ovarian afunction (OA), the first service conception rate (FCR) and pregnancy rate (PR). The pregnancy rate was 2.67 in the group of lean cows and this was higher (p ≤ 0.05) than in the other groups of cows. The highest FCR of 46.3% was recorded in fat cows before calving (BCS > 4.0). It was higher than in cows with BCS of 3.0 and 2.5 ap (p ≤ 0.01 and p ≤ 0.05, respectively). The lowest FCR of 11.1% was recorded in the group with BCS of 2.0. The mean BCS of cows after calving was 2.52 and its loss amounted to 0.74 points, i.e. 22%. Body condition loss did not have an effect on the frequency of ovarian afunction, ovarian cysts, the percentage of repeat breeders and the length of service period. The frequency of MET in the HBCL group was 29.5% and it differed significantly (p ≤ 0.05) from the frequency of this complication in MBCL and LBCL groups. In the HBCL group OA was significantly more frequent (at p ≤ 0.05) than in LBCL cows (8.7 vs. 2.1%), similarly as it was for corpus luteum pseudograviditatis (14.7 vs. 4.1%), while higher values were recorded both for FCR (41.4 vs. 37.3 in group II at p ≤ 0.05 and 32.6 in group III at p ≤ 0.01) and pregnancy rate (2.24 vs. 2.05 at p ≤ 0.05 in group III and 2.06 at p ≤ 0.05 in group II). In the analyzed population a total of 24.2% cows were culled. The higher the antepartum BCS of cows and the higher its postpartum loss, the higher the risk of culling due to infertility and the total culling risk, as well as the risk of death.
The study aimed at demonstration of sexual differences in morphology and innervation of the medial gastrocnemius muscle (MG) in male and female Wistar rats. The experimental project involved three stages: (1) the functional isolation and analysis of contractile properties of as many motor units (MUs) as possible, (2) recording the contraction force of the whole muscle evoked by stimulation of the sciatic nerve and, (3) the morphological examinations of MG. The experiments revealed that MG of males contained 46% of fast fatigable (FF) MUs, 40% of fast resistant (FR) MUs and 14% of slow (S) MUs, whereas in females 40% of FF, 37% of FR and 23% of S type MUs were found. The force of MUs in male muscle was higher, and the twitch time parameters were longer in males. Moreover, the muscle mass and force in males was signifi cantly higher. The relation of the muscle force to the mean value of MUs force revealed that male MG contained approximately 10% more MUs than in females. The male muscles which had approximately 1.5 times bigger mass than in females were composed of over 11 800 muscle fi bers, whereas in females 8 000 fi bers. These results strongly suggested that the average number of muscle fi bers in individual MUs of males was higher about 47% comparing to females. In addition, the mean diameter of muscle fi bers was 29% bigger in males. Finally, the cross-section area of muscle fi bers was also 15% larger in males.
INTRODUCTION: There are considerable differences in a number and density of muscle spindles in various skeletal muscles. Considerable sex differences in muscle mass and diameter of extrafusal muscle fibres suggest that muscle spindle density in muscles and morphometric properties of spindles: the diameter, the number and diameters of intrafusal muscle fibres are also different in males and females. Similar number of γ‑motoneurons in male and female rats suggest similar number of muscle spindles but their lower density in males. AIM(S): The aim of the study was to check sex differences in the number, the density and morphometric properties of muscle spindles in rat medial gastrocnemius muscle. METHOD(S): Medial gastrocnemius muscles were excised from two male and two female three-month old Wistar rats. Muscles were stored in 4% formalin solution and then cut into 10 and 20 µm slices stained with methylene blue and magenta. The light microscopy (Nikon microscope with camera and NIS Elements program) was used to calculate a number of muscle spindles in the muscle. The morphometric properties of spindles were measured on the equatorial regions. RESULTS: The number of muscle spindles is similar in male (13), in female (13–14) muscles. However, the density of spindles was different: 86–92 in males and 51–57 mg of the muscle mass per one spindle in females. There were also slight differences in a number of intrafusal muscle fibres in one spindle (in males: 4.3; range 3–8 and in females: 4.5; range 2–7). The diameters of intrafusal fibres on the equatorial regions amounted to: 6.7±2.1 µm in males and 6.6±2.6 µm in females. However, it was noticed that diameters of the male muscle spindles are larger than in the female ones: 21.4±7.3 µm and 20.2±5.7 µm, respectively. CONCLUSIONS: The main sex difference concerns density of muscle spindles which is lower in males than in females.
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