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Relatively little is known about the direct influence of acid rain (AR) on pro-and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria were not studied before. Aboveground parts of plants treated with different pH variants of AR and prooxidative changes (lipid peroxidation) as well as antioxidative enzyme activities (ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of prooxidative alterations was closely connected with the cell com partment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxides formation was observed in the mitochondrial fraction. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defense of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.
Relatively little is known about the direct influence of acid rain (AR) on pro- and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria have not been studied before. Aboveground parts of plants were treated with different pH variants of AR and oxidative damages (lipid peroxidation) as well as antioxidative enzyme activities (superoxide dismutase, SOD; ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of oxidative damages was closely connected with the cell compartment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxide formation was observed in the mitochondrial fraction. In both cell compartments SOD activity did not change significantly. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defence of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.
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