Wyniki wyszukiwania

1

Genetic variation of the porcine NR5A1 is associated with meat color

100%

Gorres A., Ponsuksili S., Wimmers K., Murani E.

Journal of Applied Genetics

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2016

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tom 57

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nr 1

2

Pre- and postnatal differential gene expression with relevance for meat and carcass traits in pigs - a review

100%

Wimmers K., Murani E., Ponsuksili S.

Animal Science Papers and Reports

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2010

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tom 28

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nr 2

Pre- and postnatal processes determine the final outcome of breeding of pigs in terms of traits related to carcass and meat quality at slaughter. In particular, the number of myofibers and to a large extent their metabolic and contractile properties, which also influence their size, are determined prenatally during the process of myogenesis. By this, postnatal muscle growth and parameters of meat quality are modulated. The metabolic balance, biochemical and biophysical preslaughter properties of muscle prior to slaughter determine the process of maturation of muscle to meat.Thus, differential regulation of the abundance of transcripts of biological networks in prenatal and postnatal muscle affect biochemical processes of meat maturation. In general, because the traits of interest are typically not expressed at prenatal stages, no direct relationship between phenotype and gene expression pattern can be established. However, trait-related differential expression within any prenatal developmental stage can be assessed based on known estimated breeding values, known QTL-genotypes and/or based on breed differences. Expression profiles of muscle at slaughter can directly be linked to meat quality. A suitable experimental design of “matched samples” is the discordant sib pair design. Here it is exemplarily discussed that differentially expressed transcript profiles of M. longissimus dorsi at prenatal and postnatal stages offer an insight into the biological processes in the live muscle that affect the process of meat maturation and finally meat quality.

3

Genetic aspects of feed efficiency and reduction of environmental footprint in broilers: a review

100%

Sell-Kubiak E., Wimmers K., Reyer H., Szwaczkowski T.

Journal of Applied Genetics

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2017

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tom 58

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nr 4

4

Genetic variants of major genes contributing to phosphate and calcium homeostasis and their association with serum parameters in pigs

76%

Just F., Reyer H., Murani E., Ponsuksili S., Oster M., Wimmers K.

Journal of Applied Genetics

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2018

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tom 59

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nr 3

5

Gene expression profiling by cDNA-AFLP and identification of differentially expressed transcripts of bovine pituitary gland in growing bulls of dairy breeds

52%

Pareek C. S., Pierzchala M., Michno J., Szymanska S., Smoczynski R., Jasik N., Golebiewski M., Tyburski J., Urbanski P., Goluch D., Oprzadek J., Zwierzchowski L., Siriluck P., Wimmers K.

Animal Science Papers and Reports

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2012

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tom 30

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nr 2

The study utilized age-dependent breed-specific gene expression profiling by cDNA-AFLP technique in identification of differentially expressed (DE) candidate genes for growth and development trait. Results revealed 15579 transcript derived fragments (TDFs) expressed in bovine pituitary gland tissue using 96 unique PCR reactions with primer combinations (PC) of TaqI-MseI for Polish Holstein (PH) and Polish Red (PR) dairy breeds. Gene expression profiling by cDNA-AFLP identified 1451,2877 and 4084 identically displayed (iDD), differentially displayed (DD) and single displayed (sDD) TDFs, respectively. In all transcript profiles, frequencies of DD TDFs were higher than that of iDD TDFs. A total of 60 DD TDFs bands were excised for PH (n=40) and PR (n=20) . Direct sequencing results revealed identification of 24 and 8 DE-TDFs sequences in Polish HF and PR. Based on the significance of BLAST and sequence alignment score, analysis 12 and 4 DE-TDFs sequences of PH and PR were excluded. Overall, 12 Polish HF and 4 PR breed specific DE-TDFs sequence expressed in bovine pituitary gland tissue were identified. The identified DE-TDFs sequences were represented in all bovine chromosomes, except BTA3, 4, 6, 7, 8, 9, 11, 12, 17, 18, 21, 22, 23, 25 and 26. TDF annotation results identified eight sequences that have BLAST hits to known annotated bovine genes and eight sequences to unannotated contig regions in latest gene ensemble database Btau 4,0. Two breed-specific target genes i.e., bovine glycophorin C (PH) and bovine arachidonate 5-lipoxygenase (Alox5) isoform 1 (PR) were validated by qRT-PCR. Within breed the age-dependent qRT-PCR analysis revealed that expression levels were differed significantly high (P<0.0001) with nine folds higher expression in young bulls at the age of 6 month (glycophorin C) and 9 month (Alox5 isoform 1). Between breed the qRT-PCR analyses revealed that the expression levels were highly significant for glycophorin C gene in PR and Alox5 isoform 1 gene in both breeds. It was concluded that gene expression profiling by cDNA-AFLP is a reliable technique for identification of trait-associated DE candidate genes, and helpful in elucidating and understanding molecular basis of postnatal growth and development of cattle.

6

Genome-wide identification of allele-specific expression in response to Streptococcus suis 2 infection in two differentially susceptible pig breeds

52%

Wu H., Gaur U., Mekchay S., Peng X., Li L., Sun H., Song Z., Dong B., Li M., Wimmers K., Ponsuksili S., Li K., Mei S., Liu G.

Journal of Applied Genetics

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2015

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tom 56

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nr 4

Ograniczanie wyników

Genetic variation of the porcine NR5A1 is associated with meat color

Pre- and postnatal differential gene expression with relevance for meat and carcass traits in pigs - a review

Genetic aspects of feed efficiency and reduction of environmental footprint in broilers: a review

Genetic variants of major genes contributing to phosphate and calcium homeostasis and their association with serum parameters in pigs

Gene expression profiling by cDNA-AFLP and identification of differentially expressed transcripts of bovine pituitary gland in growing bulls of dairy breeds

Genome-wide identification of allele-specific expression in response to Streptococcus suis 2 infection in two differentially susceptible pig breeds