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1

The role of nitrilotriacetic acid as a modyfying factor of phytoplankton growth

100%
Kosakowska A.
Oceanological Studies
|
1998
|
tom 27
|
nr 1
2

The effect of amino and polycarboxylic acids on the toxicity of cobalt to phytoplankton cells

100%
Kosakowska A.
Oceanological Studies
|
1996
|
tom 25
|
nr 4
3

Cyanobacterial and microalgal bioactive compounds – the role of secondary metabolites in allelopathic interactions

63%
Zak A., Kosakowska A.
Oceanological and Hydrobiological Studies
|
2016
|
tom 45
|
nr 1
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Purification and characterisation of ferritin from the Baltic blue mussel Mytilus trossulus

63%
Potrykus J., Kosakowska A.
Oceanologia
|
2009
|
tom 51
|
nr 4
Baltic blue mussels Mytilus trossulus were collected from the Gulf of Gdańsk (southern Baltic Sea) in order to isolate ferritin from its soft tissues, as well as to purify and characterise this protein. Proteins were isolated from the inner organs of M. trossulus (hepatopancreas, gills and soft tissue residue) by thermal denaturation (70◦C) and acidification (pH 4.5) of the homogenates, followed by ammonium sulphate ((NH4)2SO4) fractionation. The ferritin was then separated by ultracentrifugation (100 000×g, 120 min.). The protein content in the purified homogenates was determined by the Lowry method using bovine serum albumin (BSA) and horse spleen ferritin (HSF) as standards. PAGE-SDS and Western blotting analysis permitted identification of ferritin in the purified preparations. Additionally, the purified homogenates and mussel soft tissue were analysed for their heavy metal contents (especially cadmium and iron) in a Video 11 E atomic absorption spectrophotometer, following wet digestion of the samples (HNO3/HClO4). The electrophoregrams showed that the inner organs of M. trossulus contained ferritin, which, like plant ferritin, is characterised by the presence of subunits in the electrophoregram in the 26.6–28.0 kDa range. The highest ferritin content was recorded in the hepatopancreas, followed by the gills and the soft tissue residue. With regard to the sampling stations, the highest content of ferritin was noted in the animals sampled off Sopot (station D3), and in those collected by a diver off Jastarnia (W1) and Gdynia (W4). Ferritin isolated from the inner organs of mussels collected from these stations also contained the largest quantities of heavy metals (Cd and Fe). Ferritin isolated from the inner organs of mussels collected by a diver from wrecks – sites where the concentrations of iron and other trace metals in the sea water are high – contained higher quantities of heavy metals (Cd and Fe) than the ferritin isolated from the inner organs of mussels collected with the drag. This confirms that ferritin is a protein able to store and transport not only iron, but also, though to a lesser extent, some other heavy metals, including cadmium.
5

Effects of siderophores and amino acids on the growth and photosynthesis of populations of Chlorella vulgaris Beijerinck and Anabaena variabilis Kutzing

63%
Surosz W., Kosakowska A.
Oceanologia
|
1996
|
tom 38
|
nr 4
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Is iron a limiting factor of Nodularia spumigena blooms?

63%
Paczuska L., Kosakowska A.
Oceanologia
|
2003
|
tom 45
|
nr 4
It is well known that a deficiency of iron, a trace element essential to every living organism, limits the growth of algae and cyanobacteria. Nodularia spumigena Mertens is a blue-green algae species inhabiting the Baltic region that often forms toxic blooms. The aim of the study was to assess the growth of the toxic cyanobacteria with respect to iron bioavailability. The measured growth parameters were the numbers of cells (optical density), chlorophyll a and pheopigment a concentrations. The iron concentrations used ranged from 10−7 to 10−4 mol dm−3. Under iron stress conditions (< 5 × 10−7 mol dm−3), growth inhibition, gradual pigment decay and cell mortality were observed. However, enriching the medium with complexing factors like citric acid and EDTA significantly stimulated the growth rate and chlorophyll a production. The citric acid – EDTA – Fe (5 × 10−7 mol dm−3) complex was demonstrably effective in stimulating the rate of cell division. Starting with 10−6 mol dm−3, the higher the iron(III) concentration used in the media, the more intensive the growth of the cyanobacteria populations. This was most rapid in the presence of high iron concentrations (10−4 mol dm−3), regardless of the presence of complexing agents. It appears that the growth of toxic cyanobacteria N. spumigena, and thus also its ability to form blooms, may well depend on iron availability in the environment.
7

Changes in phytoplankton pigments composition during 2000 in different Southern Baltic Regions

63%
Ston J., Kosakowska A.
Oceanological Studies
|
2002
|
tom 31
|
nr 1-2
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of iron limitation on cells of the diatom Cyclotella meneghiniana Kützing

63%
Lewandowska J., Kosakowska A.
Oceanologia
|
2004
|
tom 46
|
nr 2
The response of the Baltic diatom Cyclotella meneghiniana to iron deficiency was examined. The following growth parameters were measured: cell number, chlorophyll a and protein content. The results demonstrate the ability of this diatom to grow well with minimal iron availability; however, the rate of growth fell markedly at the lowest iron(III) concentration. The results of spectrophotometric chlorophyll a measurements and protein assays using the Lowry and Bradford methods indicated a significant decrease in their quantities. Iron may therefore be an important regulatory factor controlling the growth of diatom C. meneghiniana in an aquatic ecosystem.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Qualitative and quantitative analysis of Baltic phytoplankton pigments

63%
Ston J., Kosakowska A.
Oceanologia
|
2000
|
tom 42
|
nr 4
The paper presents the results of research into the pigment composition of seawater samples taken from various depths in the Gulf of Gdańsk in April and September 1999. Pigments were separated by RP–HPLC, and identification was confirmed by co-injection with reference standards and on-line diode array spectra. The following groups of pigments were identified: chlorophylls: a, b, c1 + c2; photosynthetic carotenoids (PSC) – peridinin, fucoxanthin, α-carotene; photoprotecting carotenoids (PPC) – diadinoxanthin, alloxanthin, zeaxanthin, lutein, neoxanthin, violaxanthin and β-carotene. Quantitative and qualitative diversity in pigment characteristics were observed in both seasons, though lutein and α-carotene were not identified in the September samples. The respective April and September concentrations of the functional groups of pigments were: total chlorophylls content 0.47–104.25 and 0.57–13.66 μg dm−3; PSC 0.07–21.23 and 0.02–1.56 μg dm−3; PPC 0.05–14.08 and 0.04–1.79 μg dm−3. Within the PSC group, peridinin and fucoxanthin were dominant in the April samples, but only fucoxanthin in the September ones. Among the PPCs, diadinoxanthin and alloxanthin were dominant in April, zeaxanthin in September. Photosynthetic and photoprotecting carotenoids display a linear correlation with chlorophyll a content in all the samples.
10

The marine diatom Cyclotella meneghiniana Kutzing as a producer of siderophore-like substances

63%
Cabaj A., Kosakowska A.
Oceanological and Hydrobiological Studies
|
2005
|
tom 34
|
nr 1
11

Qualitative and quantitative changes of phytoplankton pigments under iron stress

51%
Lewandowska J., Kosakowska A., Ston J.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 3 suppl.
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The influence of biotic factors on phytoplankton pigment composition and resources in Baltic ecosystems: new analytical results

51%
Ston-Egiert J., Lotocka M., Ostrowska M., Kosakowska A.
Oceanologia
|
2010
|
tom 52
|
nr 1
101-125
13

Ocena aktywności przeciwnowotworowej ekstraktów polisacharydowych wyizolowanych z roślin z Morza Bałtyckiego, na podstawie wyników uzyskanych podczas prowadzenia terapii przeciwnowotworowej u myszy BALB/c zaszczepionych rakiem płuc Madisonem 109

51%
Paradziej-Lukowicz J., Kosakowska A., Falkowski L.
Prace i Materiały Zootechniczne
|
2008
|
tom 66
14

Comparison of two spectrophotometric methods of chlorophyll “a” determination in the sea water samples

51%
Bekasova O. D., Konovalov B. V., Kosakowska A., Kaczmarek S.
Oceanologia
|
1990
|
tom 28
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Skutki zmiany inwestora w przedsiębiorstwie branży tytoniowej w opinii pracowników

51%
Gebska M., Grontkowska A., Kosakowska A.
Roczniki Naukowe Ekonomii Rolnictwa i Rozwoju Obszarów Wiejskich
|
2016
|
tom 103
|
nr 2
Celem opracowania jest przedstawienie opinii dotyczących procesu prywatyzacji wyrażonych przez respondentów – pracowników przedsiębiorstwa, w którym nastąpiła zmiana inwestora zagranicznego. Z badań przeprowadzonych wśród 100 pracowników przedsiębiorstwa funkcjonującego w branży tytoniowej metodą sondażową w 2015 roku z użyciem kwestionariusza ankiety wynika, że respondenci korzystnie oceniali oddziaływanie kapitału zagranicznego na polską gospodarkę, sytuację gospodarczą miasta Radom oraz na przedsiębiorstwo ich zatrudniające. Pracownicy po zmianie inwestora najbardziej obawiali się zwolnienia z pracy, likwidacji przedsiębiorstwa i pogorszenia warunków płacowych.
16

Evaluation of primary production of phytoplankton based on chlorophyll delayed fluorescence in sea water

51%
Zvalinsky V. I., Bekasova O. D., Kaczmarek S., Kosakowska A.
Oceanologia
|
1990
|
tom 28
17

The influence of gibberellic acid on the iron uptake by the Baltic phytoplankton

51%
Malewicz B., Kentzer T., Kosakowska A., Gedziorowska D.
Oceanologia
|
1979
|
tom 11
18

Light-absorbing capacity of phytoplankton in the Gulf of Gdansk in May, 1987

51%
Konovalov B. V., Belyayeva G. A., Bekasova O. D., Kosakowska A.
Oceanologia
|
1990
|
tom 28
19

Response of phytoplankton cells to iron stres

51%
Kosakowska A., Lewandowska K., Lewandowska J.
Acta Physiologiae Plantarum
|
1999
|
tom 21
|
nr 3 suppl.
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Heterotrophic bacteria from brackish water of the Southern Baltic Sea: biochemical and molecular identification and characterisation

51%
Cabaj A., Palinska K., Kosakowska A., Kurlenda J.
Oceanologia
|
2006
|
tom 48
|
nr 4
Six bacterial strains isolated from the surface water of the southern Baltic Sea were described on the basis of their morphological, physiological and biochemical features, and were classified on the basis of 16S rDNA sequence analysis. Comparative analyses of the 16S rDNA sequences of five of the six bacterial strains examined displayed a ≥98% similarity to the sequences available in the NCBI GenBank. The 16S rDNA sequence of strain 2 shared only a 96% similarity with other published sequences, which suggests that this is a new, hitherto unknown species. The isolated heterotrophic bacteria belong to the families Bacillaceae (strain 1), Flexibacteriaceae (strain 2), Sphingomonadaceae (strains 3, 5), Micrococcaceae (strain 4) and Aurantimonadaceae (strain 6). This is the first study in which the polyphasic approach has been applied to the identification of heterotrophic bacteria from the brackish waters of the Gulf of Gdańsk and Gdańsk Deep.
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