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Sucrose is the principal form of photosynthesis products, and long-distance transport of sucrose requires sucrose transporters (SUTs) to perform loading and unloading functions. SUTs play an important role in plant growth, development and reproduction. In this study, five unique sucrose transporter (SbSUT) genes that contain full-length cDNA sequences were cloned from sweet sorghum, and these SbSUT genes were clustered into four different clades: SUT1, SUT3, SUT4 and SUT5. Heterologous expression of SbSUTs in yeast demonstrated that they were functional sucrose transporters. Tissue-specific expression profiles showed that sorghum SUT genes had different tissue-specific expression patterns, suggesting that sorghum SUT genes may play an important role in plant growth and developmental processes. After defoliation, expression patterns of SbSUT1, SbSUT2 and SbSUT4 were different in leaf sheaths, leaves and roots. Taken together, the results indicate that the above mentioned five unique sucrose transporter genes may play important roles in performing sucrose loading and unloading functions and that they exhibit different expression in response to leaf blade removal.
The degraded ecosystem in the Loess Plateau region of China is very vulnerable, which has a great impact on regional sustainable development. Taking the Loess Plateau of Yan’an, Shaanxi province, China, as the study area, this study quantitatively evaluated conditions of the ecological environment. Based on natural factors, environmental factors, and socio-economic factors of Yan’an in 1997, 2004, and 2011, the dynamic changes in ecological vulnerability are analyzed with the application software for geographic information systems (GIS). The analytic hierarchy process (AHP) model was used and it contains variables for land use, soil erosion, topography, climate, vegetation, and socio-economic data. The evaluation results are divided into fi ve categories: potential, slight, light, medium, and heavy. The results show that the ecological vulnerability in the study is light level, and the ecological vulnerability index of the southern four counties is lower than that of the northern four counties. Ecological environment quality gradually improved in most areas in 1997~2011.
For more efficient utilization of agricultural organic waste composts, this research formulates the demand of agricultural organic waste composts and the supply of compost converted from agricultural organic waste in the region, and models the demand-supply balance of agricultural organic waste compost in some regions. Objects of study include 6 urban districts, 5 county-level cities, and 21 counties in northern Henan Province, China. The crops’ latent demand for compost and the latent supply of compost converted from agricultural organic wastes (straw and livestock manure) in each region is computed for an analysis of demand-supply balance. According to the findings, there is a large deviation in both the ratio of the supply of livestock manure composts to that of straw composts and the demand-supply ratio of composts in the studied regions. To be specific, the overall demand-supply ratio in the studied regions is about 1.21, showing that demand is greater than supply. Theoretically, all agricultural organic wastes can be used for agricultural production in these regions. In addition, actual demand ratio (actual demand/ latent demand) is found in the utilization of composts. Supposing the actual demand ratio is 50%, research again analyzes the demand-supply balance, revealing that all livestock manure composts can be utilized, but that straw compost is surplus in large amounts in the studied regions.
Background: Early life adversity (ELA) is not uncommon in major depressive disorder (MDD) patients. Childhood trauma has been reported more frequently in adult MDD patients relative to healthy controls. Recent researches have demonstrated that ELA could result in changes in brain morphology which might be an aetiological factor of MDD development. Materials and methods: We recruited 40 young adult patients suffered from MDD and made computed tomography scan. Subjects were divided in two groups: MDD patients with ELA experience (E+D) vs. MDD patients without ELA experience (E–D) according to Chinese version-Childhood Trauma Questionnaire (CTQ). 17-item Hamilton Depression (HAMD) Scale and Neuropsychiatric Inventory (NPI) were also examined. Student’s t-test was used to compare the HAMD scores, NPI scores, CTQ subcomponents scores, third ventricular (TV) width and volume of patients from E+D and E–D groups. Results: Findings demonstrated that ELA might result in TV enlargement; furthermore, there was a correlation between physical neglect and TV volume. Conclusions: These findings supported the hypothesis that ELA could induce changes of structure around the TV, which might undermine the aetiology of MDD. (Folia Morphol 2018; 77, 3: 428–433)
The genus Malaxa Melichar, 1914 (Hemiptera: Fulgoroidea: Delphacidae: Tropidocephalini) feeding exclusively on bamboo (Bambusoideae), containing 4 species: M. delicata Ding et Yang, 1986 (Guizhou: Guiyang; Yunnan: Mongban; Zhejing: Hangzhou; Fujian: Huangkeng), M. hunanensis Chen sp. nov. (Hunan: Zhangjiajie), M. fusca Yang et Yang, 1986 (Taiwan: Nantou) and M. semifusca Yang et Yang, 1986 (Taiwan: Nantou; Hualian; Guizhou: Daozhen) from China is here reviewed. The male of M. fusca Yang et Yang, collected from Nantou County of Taiwan, is reported and described for the first time. The main morphological characters and male genitalia of 4 species are described and illustrated. A key to species in the genus from China is provided.
Applying nitrogen (N) to increase alfalfa (Medicago sativa L.) yield is a general practice in China that has caused serious weed infestation problems. Our objective was to determine the effect of N fertilizer and seeding rate on yields of alfalfa and weeds. The effects of four N fertilizer gradients (0, 50, 100, and 150 kg N hm⁻²) and five alfalfa seeding rates (0, 5, 7.5, 10, and 15 kg hm⁻²) were examined on dry matter of alfalfa and weeds using a three-year in situ controlled trial. The results showed: • There were no significant differences among N fertilizer (N) for dry matter of alfalfa (DMA) during three years. Dry matter of grassy weeds (DMGW) and broadleaf weeds (DMBW) were significantly increased with the N fertilizer gradient in the seeding year. • DMA was significantly increased with the seeding rates gradient in both 2012 and 2013. DMGW and DMBW were significantly decreased with the seeding rates gradient in all three years. • Interactions between N fertilizer and seeding rates on DMA and DMBW in 2012 and DMGW and DMT in 2013 are significant (p<0.05). Although the study demonstrated that increasing seeding rate can suppress weed invasions, N fertilizer facilitated them.
The Xilin Gol area is located in central Inner Mongolia, China. It had been a fertile prairie in northern China, but desertification and sand storms have increased in past decades. This study shows the spatial and temporal distribution of sand storms in the Xilin Gol area. The results of the analysis show: (i) Factors such as strong winds (usually beyond seven levels), rain and temperature have an impact on sand storms. The correlation coefficient between sand storm days and strong wind days is 0.89. (ii) There are more sand storm days in the west than in the east. (iii) In the last 30 years, the frequency of sand storms have been consistently decreasing.
Dynamic properties are vital for the working reliability of aft stern tube bearings. However, the determination of such properties currently involves several simplifications and assumptions. To obtain its dynamic characteristics accurately, the aft stern tube bearing was divided into several bearing segments. The oil film reaction force was considered in the calculation of shaft alignment, and the journal deflection and actual oil film thickness were obtained accordingly. Subsequently, the perturbed Reynolds equation was solved using the finite difference method when the dynamic characteristics of journal bearings with finite width were evaluated. Then, a calculation program was developed and verified by comparing with the results of other studies. Finally, the dynamic characteristics were calculated under different revolutions. The results showed that the stiffness at the vertical direction of the aft stern tube bearing was several times that of the horizontal direction and varied with the revolutions of the shafting system. These findings can provide the foundation for the precise calculation of the journal trajectory under dynamic conditions, as well as for the evaluation of the oil film thickness. Moreover, the results led to favorable conditions for the accurate calculation of the shafting whirling vibration
Human activity has greatly increased the amount of biologically available nitrogen entering the natural environment. Addition of N can affect growth of and competitive interactions between native and invasive plants, thus increasing or decreasing the risk of invasion by alien species. Bidens frondosa is an invasive weed native to North America that recently has begun to spread in China. The influence of soil nutrient content on B. frondosa invasion has not yet been reported. In a common garden experiment, we compared the growth and competitive effects between B. frondosa and its co-occurring native congeners — B. tripartita and B. biternata — under three N levels (0 g m⁻², 1 g m⁻², 5 g m⁻²) to assess whether increased levels of N modifies risk of B. frondosa invasion. Our results showed that while N additions increased both the growth and competitive advantage of B. frondosa as compared with that of the native congener species, results are particularly pronounced under high N levels. While growth responses to N addition varied little among invasive populations under high N levels, the competitive effect of B. frondosa did vary among populations and was significantly greater than that of the congeners. Anthropogenic N additions are likely to increase risks of B. frondosa invasions. Thus, management efforts should focus on the reduction of N input to ecosystems to mitigate invasions by B. frondosa.
Adjuvant chemotherapy has become the frequently adopted standard therapeutic approach for non-small cell lung cancer (NSCLC). However, the development of multidrug resistance (MDR) is a major obstacle contributing to the failure of chemotherapy. This study aimed to identify genes associated with MDR development that predict tumor response to chemotherapy in NSCLC. In the present study, a multidrug-resistant NSCLC cell sub-line, A549/MDR, was established from the A549/DDP cell line and characterized. The resistance index (RI) of this subline was calculated according to the IC50 of A549/MDR relative to the parental A549/DDP cells. The gene expression profiles of A549/DDP and A549/MDR were obtained using an oligonucleotide microarray (Agilent SureHyb microarray chip). The microarray results were validated by qRT-PCR and selected genes were analyzed by in vitro loss-of-function experiments. Gene expression profiling identified 921 differentially expressed genes (DEGs) according to the selection criteria, in which 541 genes were upregulated and 380 genes were downregulated in A549/MDR compared with A549/DDP cells. We found that these DEGs are involved in diverse biological processes, including ribonucleoprotein complex, drug metabolism, the Hippo signaling pathway and transcriptional misregulation. NOLC1, as one of the identified DEGs, was confirmed to be overexpressed in A549/MDR cells and its knockdown significantly enhanced the drug sensitivity of A549/MDR cells in response to multidrug treatment. Furthermore, knockdown of NOLC1 downregulated the expression levels of drug resistance-associated molecules (LRP and MDR1) in A549/MDR cells. These findings provide a new and comprehensive expression profile of MDR in NSCLC cells. Identification and validation of NOLC1 might be a promising therapeutic strategy for the management of MDR of NSCLC patients.
Green high performance fiber reinforced cementitious composites (GHPFRCC) are a new class of sustainable cementitious composites, employing a high volume of fly ash to replace cement. In addition to increasing the sustainability of the construction environment, GHPFRCC exhibits a high tensile ductility and multiple cracking behaviors in the strainhardening state. These materials can effectively improve the structural energy dissipation capacity and structural durability. In this study, the optimum mixture ratio of GHPFRCC is presented established using an orthogonal experiment for a specific engineering application. The described GHPFRCC sustains the mechanical performance of concrete and is employed as the outer cladding to strengthen concrete columns. The finite element analysis of the material was based on the software ABAQUS and pseudo static experiments were conducted to exhibit retrofitting of GHPFRCC applied in the rehabilitation of seismic-damaged concrete columns. The computed and experimental results showed that GHPFRCC, while incorporating high volume fly ash, can retain significant multiple cracking behaviors. The energy dissipation capacity of the GHPFRCC reinforced concrete (RC) column is better than the comparable unreinforced column
Tetrabromobisphenol-A (TBBPA) and its analogue compounds (bisphenol-A (BPA) and 2,6-dibromophenol (2, 6-DBP)) sorption on CTMAB-clays was investigated in this paper through bath experiments. Montmorillonite and kaolinite modified by CTAMB, as the adsorbent, were firstly characterized by XRD, FTIR and SEManalysis. The surface areas was determined using N2 (77K) sorption-desorption analysis. Kinetic studies showed that sorption reached equilibrium in 5 hours and followed the pseudosecond order kinetic model. The intra-particle diffusion model for sorption was also investigated and compared to identify the sorption mechanism. The sorption isotherms, well fitted by Freundlich model, were changed from being non-linear to being linear with intercalating CTMAB into clays, which indicated that CTMAB increased the partition interaction. A combination of partition and specific sorption might be contributed to TBBPA and its composition products sorption mechanism. The Freundlich coefficients (kF) and distribution coefficients (kd) for both two adsorbents were primarily increased by increasing amount of adsorbed CTMAB, while the organic carbon normalized sorption coefficients (koc) did not follow this trend. Meanwhile, 100%CEC-CTMAB-Montmorillonite showed an advantage of removal of the selected compounds than 100%CEC-CTAMB-Kaolinite. The sorption capacity of BPA was bigger than that of 2,6-DBP in spite of their similar hydrophobicity, which indicated some molecule properties might influence the sorption on CTMAB-clays. Moreover, the negative ΔG0 and ΔH0 indicated the spontaneous and exothermal process. The TBBPA sorption on CTMAB-clays was considerably enhanced in the acid condition.
The effects of 6-benzyladenine (6-BA) on plant growth, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant systems of eggplant (Solanum melongena L.) under salt stress were investigated. Eggplant seedlings were exposed to 90 mM NaCl with four levels of 6-BA (5, 10, 20 and 50 µM) for 10 days. 6-BA at lower concentrations increased chlorophyll concentration, the net photosynthetic rate (PN), stomatal conductance (gs), and transpiration rate (E), intercellular CO₂ concentration (Ci) and water use efficiency (WUE), as well as the quantum efficiency of PSII photochemistry (UPSII), photochemical quenching (qp), and decreased non-photochemical quenching (NPQ), while higher concentrations reduced the effects or even exacerbated the occurrence of photosynthetic capacity. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) increased significantly during salt treatments, and induced the increase of the activities of these enzymes at certain concentrations of 6-BA. 6-BA also reduced significantly malonaldehyde (MDA) contents and O₂⁻ production. It was concluded that 6-BA could alleviate the detrimental effects of salt stress on plant growth by increasing photosynthetic efficiency and enhancing antioxidant enzyme systems in leaves at a proper concentration and of the varying 6-BA concentrations used, the most effective concentration for promoting growth was 10 µM under saline conditions.
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