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C-reactive protein (CRP) has two structurally distinct isoforms, the CRP pentamer and the CRP monomer. A role for the CRP monomer in atherosclerosis is emerging, but the underlying mechanisms are only beginning to be understood. Monocytes are an important contributor to atherosclerosis, and foam cell formation is the hallmark of atherogenesis. However, whether the CRP monomer can directly interact with the monocytes and modulate their responses remains unknown. Furthermore, although FcγRIII (CD16) has been identified as the receptor for the CRP monomer on neutrophils, its role in mediating the CRP monomer’s biological effects in other cell types has been questioned. In this study, we investigated the interaction of the CRP monomer with the monocytes using the U937 monocytic cell line. The CRP monomer specifically binds to U937 cells. This binding is unique in that it is independent of FcγRs and insensitive to protease digestion of the cell surface proteins. Further assays revealed that the CRP monomer directly incorporates into the plasma membrane. Interestingly, the presence of the CRP monomer efficiently retards oxidized low-density lipoprotein-induced foam cell formation of PMA-differentiated U937 macrophages and peripheral blood monocytic cell-derived macrophages. These findings provide additional evidence for the notion that the CRP monomer is an active CRP isoform that plays a role in atherogenesis via the direct modulation of the behavior of the monocytes.
n view of the characteristics of low speed mobile ship network, strong network dynamics and large signal interference, this paper proposes a low-rate mobile sensor network routing algorithm. In mobile wireless sensor networks, network topology changes frequently is a new challenge for resource constrained sensor node. Constantly updated network topology, sensor nodes need to exchange data frequently in order to maintain fresh route, resulting in sensor nodes consume a lot of energy. To solve this problem, to reduce control overhead is considered to be an effctive strategy. In this paper, we present a vector network-based dynamic routes (VNDR) scheme aims to minimize the control cost of the sensor nodes. We propose data exchange based on binary-coded vector address to reduce the control cost, moreover, we present a set of communication rules that govern the routes search procedure thereby requiring little cost of packet analysis to determine the next hop of node. VNDR performance was compared with well known scheme such as SPIN and DD. Simulation results show that, our VNDR scheme reduced the network control overhead and improved network lifetime
In order to analyze the influential factors of soft foundation settlement in a marine reclamation land project, the consolidation settlement and pore pressure dissipation of the entire area are numerically simulated using Soft-SoilCreep Model, in which the PLAXIS finite element software for professional geotechnical engineering is applied and empirical data of Japanese Kansai’s airport project are used. Moreover, the figures of settlement and pore pressure results in the different basic period are drawn, and the corresponding analysis conclusions are ob-tained based on the comparison among the results from the computational parameters of depth. In addition,, the influence rules of various parameters on settlement results is concluded through running the parameter sensitivity analysis in Soft-Soil-Creep Model, and the experience and conclusions can be for reference in the design and con-struction of similar large-scale marine reclamation land project. Also the empirical value method of the creep index has not been applied widely. Further research needs to be done
To give full play to the circulation function of the port container logistics park, it is urgent to study the development intensity of the land in the port container logistics park and to guide the scientific development of the port logistics park with reasonable development intensity control index. The current situation of land development intensity control index of container logistics park at home and abroad is analysed, the PENT (politics, economy, society and technology) analysis method is used to analyse the factors influencing the land development intensity control index of container logistics park, and the index system structure of influencing factors is constructed. Finally, index value is obtained quantitatively with the proposed calculation method of the land development intensity index of the port container logistics park. Its practicability is verified in case analysis
The effects of six emergent macrophytes (Typha orientalis, Acorus calamus, Oenanthe javanica, Scirpus validus, Sagittaria sagittifolia, and Pontederia cordata) on the growth of two strain Microcystis aeruginosa were studied under co-culture conditions. And the sensitivities of unicellular and colonial Microcystis strains to six emergent macrophytes were compared using an exudation experiment. Based on laboratory experiments, T. orientalis, A. calamus, O. javanica, S. validus, S. sagittifolia, and P. cordata had strong inhibitory effects on growth of unicellular M. aeruginosa, while only A. calamus and P. cordata show obvious growth inhibition on colonial M. aeruginosa. When the biomass density was 20 g FW·L⁻¹, the growth inhibition rate of unicellular M. aeruginosa can exceed 90% for all of the six emergent macrophytes. When macrophytes coexisted with the colonial M. aeruginosa, only A. calamus, P. cordata, and S. sagittifolia showed the growth inhibition of algae. Maximal inhibition of Chl a growth was 75% (p<0.05) for A. calamus, 69% (p<0.05) for P. cordata, and 40% for S. sagittifolia at 45 g FW·L⁻¹ on day 15. The results of the exudation experiment indicated that there were no significant differences between control and treatment of Chl a concentrations of colonial M. aeruginosa for all of the six macrophyte exudations on days 6 and 12. While after 6 d incubation in 100% and 50% macrophyte exudations (40 g FW·L⁻¹), the cell densities of unicellular M. aeruginosa in control were obviously higher than all those in treatment (p < 0.05). The maximal algal growth inhibition (89.62%) of unicellular M. aeruginosa was achieved in 100% exudation of A. calamus on day 6 (p < 0.05). So according to the results of exudation experiments, the unicellular M. aeruginosa was more sensitive than the colonial strain to six emergent macrophytes. And this different sensitivity between Microcystis species probably correlated positively with colony size.
Ground liquefaction and deformation is one of the important causes that damage engineering structures. Chinese current code for seismic design of breakwater is based on the single-level seismic design method as well as code for port and water-way engineering. However, this code can not exactly reflect the seismic performance of breakwater structures which experience different seismic intensities. In this paper, the author used a finite difference software, namely, FLAC3D, to analyze the state and compute seismic responses of breakwater structure. The breakwater foundation’s pore pressure ratio and displacement due to different earthquake have been studied. And the result show that: Smaller earthquakes have little influence on serviceability of the foundation, and severe earthquakes can liquefy some parts of the foundation; In the latter case , obvious changes of pores and foundation displaces can be found. Particularly, when seismic peak acceleration reachs 0.2g, Liquefaction appears in the foundation and mainly concentrated in the upper right side of the structure. In addition, the survey of ultra-hole pressure and displacement values of sand layers of the breakwater, manifests when the ultra pore pressure near 1.0, displacement and overturning structure is relatively large, resulting in varying degrees of damage to the structure. This paper’s research can provide theoretical and designable reference for similar engineering structures
Medicago sativa L. cv. Longzhong is a nutritious forage plant in dryland regions of the Loess Plateau with strong drought tolerance and broad adaptability. To understand the adaptation mechanism of alfalfa (M. sativa L. cv. Longzhong) to drought stress, growth, and physiological parameters including levels of chlorophyll content, osmotic adjustment, reactive oxygen species (ROS), and antioxidant enzymes and antioxidants were measured under simulated levels of drought (− 0.40, − 0.80, − 1.20, − 1.60, and − 2.00 MPa). The changes in M. sativa L. cv. Longzhong were compared with those of plants of M. sativa L. cv. Longdong control (Variety I) suited to moderate rainfall areas and M. sativa L. cv. Gannong No. 3 (Variety II) suited to irrigated areas. The results showed that root–shoot ratio, the chlorophyll (a + b) and osmolytes contents, the degree of lipid peroxidation and ROS production, and the levels of antioxidative enzymes and antioxidants increased significantly with increasing drought stress, whereas plant height, aboveground biomass, chlorophyll a/b ratio, leaf water potential (Ψ₁), and relative water content (RWC) decreased in response to drought. The Longzhong variety responded early to beginning drought stress (between 0 and − 0.4 MPa) compared with the controls. Under drought stress (between − 0.4 and − 2.0 MPa), the Longzhong variety had significantly higher belowground biomass, root–shoot ratio, Ψ₁, RWC, catalase (CAT) activity and reduced glutathione content than those of Varieties I and II, but hydrogen peroxide and hydroxyl free radical (OH·) contents were significantly lower. Step regression analysis showed that OH·, CAT, malondialdehyde, superoxide anion-free radical (O₂˙⁻ ), and superoxide dismutase of Longzhong had the most marked response to drought stress. In conclusion, the stronger drought tolerance of the Longzhong variety might be due to its higher water-holding capacity, root–shoot ratio, and ability to coordinate enzymatic and non-enzymatic antioxidant systems, which coordinate the peroxidation and oxidative systems.
In an artificial island construction project based on the large-scale marine reclamation land, the soil settlement is a key to affect the late safe operation of the whole field. To analyze the factors of the soil settlement in a marine reclamation project, the SEM method in the soil micro-structural analysis method is used to test and study six soil samples such as the representative silt, mucky silty clay, silty clay and clay in the area. The structural characteristics that affect the soil settlement are obtained by observing the SEM charts at different depths. By combining numerical calculation method of Terzaghi’s one-dimensional and Biot’s two-dimensional consolidation theory, the one-dimensional and two-dimensional creep models are established and the numerical calculation results of two consolidation theories are compared in order to predict the maximum settlement of the soils 100 years after completion. The analysis results indicate that the micro-structural characteristics are the essential factor to affect the settlement in this area. Based on numerical analysis of one-dimensional and two-dimensional settlement, the settlement law and trend obtained by two numerical analysis method is similar. The analysis of this paper can provide reference and guidance to the project related to the marine reclamation land
Atrial natriuretic peptide (ANP) regulates blood pressure mainly through the occupation of the guanylyl cyclase-coupled receptor NPR-A, which requires ATP interaction for maximal activation. This study investigates the effect of extracellular Ca2+ on ATP-mediated regulation of NPR-A-coupled guanylyl cyclase activity in glomerular membranes from Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR). ATP induced a significant increase in basal and ANP1-28-stimulated guanylyl cyclase activity that was greater in SHR than in WKY. Extracellular Ca2+ inhibited ATP-stimulated guanylyl cyclase activity in a concentration-dependent manner, but did not modify basal and ANP1-28-stimulated guanylyl cyclase activity. In the presence of ATP, NPR-A showed higher affinity for ANP1-28 and lower Bmax. Ca2+ did not modify NPR-A-ANP1-28 binding properties. The different effects of extracellular Ca2+ on ANP1-28- or ATP-mediated guanylyl cyclase activation suggest that these events are differentially regulated. Addition of extracellular Ca2+ induced similar effects in hypertensive and normotensive rats, suggesting that it is not responsible for the elevated cGMP production observed in SHR.
Temporal and spatial changes of calcium distribution in rice central cells were studied ultracytochemically by potassium antimonate precipitation. Before anthesis the calcium level was quite low. Calcium increased remarkably just after pollination and then decreased again gradually. Calcium precipitates were localized in the polar nuclei, including the nucleolus and nucleoplasm, and the cytoplasm surrounding polar nuclei. In the large central vacuole the calcium amount was negligible, though pollination-independent calcium elevation occurred in the chalazal region of the vacuole. During fertilization, abundant calcium was found in the fusing sperm and polar nuclei. Fertilization-induced calcium accumulation in the micropylar region of the central vacuole appeared after fertilization and during early mitosis of free endosperm nuclei. No obvious change of calcium level was observed in emasculated but unpollinated central cells, and in this case the level was much lower than in pollinated central cells. Calcium was also localized in the antipodal cells, embryo sac wall and nucellus, and no change was observed in these places before and after pollination.
The loss of biodiversity caused by wetland degradation is a hot issue in ecology. It is known that hydrological degradation is the primary reason resulting in wetland degradation, but little is known about the relationship between plant species richness and environmental factors in different wetland types along a hydrological gradient. According to the gradient from high to low water level, wetland remnants in the Sanjiang Plain of northeast China were classified into three wetland types, which were permanently inundated marshes (PIM), seasonally inundated marshes (SIM) and wet meadows (WM) respectively. In this paper, we aimed to identify the determinants of plant species richness in the three wetland types and discern the transition of the determinants along a hydrological gradient. Plant species richness as well as area, habitat heterogeneity and resource availability was investigated in 51 wetland remnants, which were composed of 6 PIM, 25 SIM and 20 WM. Averagely, the area of wetland remnants occupied by PIM, SIM and WM was 0.35 ± 0.17 ha, 2.81 ± 2.88 ha and 1.34 ± 1.18 ha respectively. Aggregating the species in each wetland type, there were 67, 244 and 170 species recorded in PIM, SIM and WM. The determinants of species richness varied in different wetland types: standing water depth in PIM, area and water heterogeneity in SIM, and soil fertility and area in WM. With the decreasing water level, the influence of hydrological condition on species richness in the three wetland types declined while the impact of area and soil fertility gradually increased. Thus, hydrological condition was probably responsible for the transition of the determinants of species richness in different wetland types. Moreover, the habitat specialists of wetland would be lost when PIM or SIM degraded to WM. In order to conserve and restore plant diversity, specific measures should be taken including preventing area loss for all wetland remnants, managing the hydrological process for PIM and SIM, and regulating soil nutrient for WM.
Background. 6-Gingerol is one of the most pharmacologically active and abundant components in ginger, which has a wide array of biochemical and pharmacologic activities. In recent years, the application of microwave-assisted extraction (MAE) for obtaining bioactive compounds from plant materials has shown tremendous research interest and potential. In this study, an efficient microwave-assisted extraction (MAE) techniąue was developed to extract 6-gingerol from ginger. The extraction efficiency of MAE was also compared with conventional extraction techniques. Material and methods. Fresh gingers (Zingiber officinale Rosc.) were harvested at commercial maturity (originally from Shandong, laiwu, China). In single-factor experiments for the recovery of 6-gingerol, proper ranges of ratio of liquid to solid, ethanol proportion, microwave power, extraction time were determined. Based on the values obtained in single-factor experiments, a Box-Behnken design (BBD) was applied to determine the best combination of extraction variables on the yield of 6-gingerol. Results. The optimum extraction conditions were as follows: microwave power 528 W, ratio of liquid to solid 26 mL g1, extraction time 31 s and ethanol proportion 78%. Furthermore, more 6-gingerol and total polyphenols contents were extracted by MAE than conventional methods including Maceration (MAC), Stirring Extraction (SE), Heat reflux extraction (HRE), Ultrasound-assisted extraction (UAE), as well as the antioxidant capacity. Conclusion. Microwave-assisted extraction showed obvious advantages in terms of high extraction efficiency and antioxidant activity of extract within shortest extraction time. Scanning electron microscopy (SEM) images of ginger powder materials after different extractions were obtained to provide visual evidence of the disruption effect. To our best knowledge, this is the first report about usage of MAE of 6-gingerol extraction from ginger, which could be referenced for the extraction of other active compounds from herbal plants.
Phytoplasmas are associated with diseases of several hundred plant species. Jujube witches’ broom disease (JWB) is a destructive phytoplasma disease in Chinese jujube (Ziziphus jujuba Mill.). Ziziphus jujuba Mill. ‘J5’, an excellent strain with extremely high resistance to JWB, was selected by us. In our previous study, a GST (EC 2.5.1.18) fragment was sequenced from suppression subtractive hybridization library of ‘J5’ under JWB phytoplasma stress. Based on this result, a GST gene (ZjGSTU1, HM345954) was first isolated from jujube by homology cloning and RACE. ZjGSTU1 contains a complete open reading frame of 702 bp encoding a protein of 233 amino acid residues. Sequence alignment showed that ZjGSTU1 shared a typical conserved structure and high identity with tau GSTs from other plant species. Relative RT-PCR demonstrated that the expression of ZjGSTU1 mRNA in jujube leaves and branches could be triggered by JWB phytoplasma. Moreover, the expression of ZjGSTU1 mRNA in resistant strain ‘J5’ increased sooner and higher than that in sensitive strain ‘J9’ under JWB phytoplasma stress (‘J5’ and ‘J9’ are two strains from the same cultivar). Western blotting analyses showed that the expressions of ZjGSTU1 in ‘J5’ and ‘J9’ were dramatically up-regulated under JWB phytoplasma stress and its expression in ‘J5’ was also higher than that in ‘J9’ at protein level. Collectively, this paper highlights that ZjGSTU1 gene is responsive to phytoplasma infection. The possible roles of this gene were discussed in terms of regulatory process in resistance to phytoplasma infection.
The ground-active arthropod diversity response to size of shrub plantations in desertified grassland ecosystems is largely unknown. In the study ground-active arthropods were collected by pitfall trapping beneath shrub canopy of very low, low, medium and high size, with adjacent mobile sandy land as a control. It was found that arthropod dominant taxa from mobile sandy land were significantly distinctive from those from plantations of different shrub size. A considerably lower Sørensen index (i.e., 0.25–0.48) was found between the arthropod communities from mobile sandy land and the canopy of either shrub size, than between those under low and medium/high shrub size (i.e., 0.62 to 0.69). The arthropod total abundance was significantly greater under the shrub canopy of very low size in comparison to that of low and medium shrub size and mobile sandy land, with the intermediate values under shrub canopy of high shrub size. Taxon richness and diversity of arthropod communities were distinctly lower under the shrub canopy of low size in comparison to very low, medium and high shrub size. The shrub size was found to have different effects on the density and richness distribution of arthropod trophic groups (i.e., predators, phytophagous, saprophagous, and omnivorous). It was concluded that shrub plantations could facilitate ground-active arthropod diversity recovery when they were afforested in mobile sandy land. There was a contrasting effect of shrub size on ground-active arthropod diversity recovery versus arthropod abundance when grazing was excluded.
Using a CIRAS-2 portable photosynthesis system, the photosynthesis, transpiration, and light responses of Aralia elata were studied under different soil moisture values with the aim of understanding the adaptability of this species to different light and soil water conditions. The results showed that the net photosynthetic rate (Pn), transpiration rate (Tr), and water use efficiency (WUE) of A. elata varied in response to different levels of photon flux density (PFD). When PFD was between 800 and 1,800 μmol m⁻² s⁻¹, Pn changed little in response to the increasing light intensity, whereas Tr decreased gradually and WUE increased significantly. Light saturation point (LSP) and light compensation point (LCP) were approximately 800 and 30 μmol m⁻² s⁻¹, respectively. The soil water content had little effect on LSP and LCP, but significantly affected photosynthetic quantum efficiency (Φ) and dark respiratory rate (Rd). By defining the Pn and WUE as the indexes of plant productivity and soil water efficiency, respectively, the soil moisture availability and productivity of A. elata were graded and evaluated, and six types of soil water grading were established. Thus, at RWC values between 44.5% and 79.2% in which both Pn and WUE were maintained at a high level, the results can provide theoretical support for highly productive and efficienct water management in A. elata.
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