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1

The participation of plant cell organelles in compatible and incompatible potato virus Y-tobacco and -potato plant interaction

100%
Otulak K., Garbaczewska G.
Acta Physiologiae Plantarum
|
2014
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tom 36
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nr 01
Potyviruses replicate and express their genomes in the cytoplasm in closely related membranous structures such as the endoplasmic reticulum or in the vicinity of the ER. The present research demonstrates the participation of plant cell organelles based on ultrastructural examination of compatible and incompatible interactions in tobacco- and potato-potato virus Y (PVY) necrotic strains. In two interaction types, PVYN Wi and PVYNTN particles were documented inside cell nuclei. Virus cytoplasmic inclusions and particles were associated with nuclear envelope pore complexes. Moreover, the PVY capsid protein was immunolocalised in the cell nucleus and nucleolus. Our results for the first time show PVY particles and capsid proteins inside the mitochondrion in compatible interactions, whereas in hypersensitive responses these interactions were identified inside chloroplasts. The PVY particles attached to mitochondria caused association groups of these organelles. The ultrastructural analysis clearly demonstrated both the dynamics of the endoplasmatic reticulum in two types of PVY interactions and connections between PVY cytoplasmic inclusions and particles with its membranous structures. Moreover, we demonstrated strongly localised immunodetection of the PVY capsid protein on the surface and in the vicinity of ER in cases of hypersensitive response as well as in compatible interaction.
2

Cell-to-cell movement of three genera (+) ss RNA plant viruses

100%
Otulak K., Garbaczewska G.
Acta Physiologiae Plantarum
|
2011
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tom 33
|
nr 2
The current investigations of three genera plant virus cell-to-cell movement were presented. Viruses reveal different local transport strategies, but all of them are the results of virus factors–host components interactions. The Tobacco mosaic virus (TMV) does not require capsid protein for translocation through plasmodesmata but 30 K movement protein participates in this process. It was found direct or indirect TMV movement proteins host partners in Tobamovirus movement like: pectin methylesterase, movement protein binding 2C, chaperones or cytoskeleton components and endoplasmatic reticulum membranes. The Potex- and Potyvirus cell-to-cell movement is closely related to replication network. The PVX capsid protein and triple gene block protein system are responsible for efficient local transport. Potyviruses move through the plasmodesmata by involving viral encoded proteins but not specific movement proteins. While the Potyvirus is the biggest known plant virus genus, host components participating in or regulating directly its plasmodesmata- movement are still not clear.
3

Ultrastructural events during hypersensitive response of potato cv. Rywal infected with necrotic strains of potato virus Y

100%
Otulak K., Garbaczewska G.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 4
Potato plants cv. Rywal with hypersensitivity gene Ny-1 infected with PVYN or PVYNTN reacted in local necroses 3 days after infection. Potato virus Y (PVY) particles were found in epidermis, mesophyll, phloem and xylem cells in inoculated leaves. Noncapsidated virus particles (without capsid protein) were observed already 10 h after infection by using electron microscopy in situ. Capsid protein on one terminus of noncapsidated virus particles was located 5 days after inoculation with the use of immunogold labeling method. Whereas cytoplasmic inclusions were observed for the first time 24 days after infection during hypersensitive response. Ultrastructural studies showed that ER may take part in PVY RNA replication and capsidation of Potyvirus particles. Observed cytopathological changes and virus particles indicate that cell nucleus and mitochondrion might participate in PVY life cycle. During hypersensitive response PVY particles were found in plasmodesmata as well as in phloem and xylem.
4
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Tobacco rattle virus (TRV) PSG influence on Capsicum annuum and Nicotiana tabacum generative organs

100%
Otulak K., Garbaczewska G.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
5

The ultrastructural comparison of compatible and incompatible potato - and tabacco - PVY NTN interaction

100%
Otulak K., Garbaczewska G.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
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tom 51
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nr 2
6
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Necrosis in Solanum tuberosum stems infected with potato virus Y by grafting

81%
felczak P., Garbaczewska G., Otulak K.
Acta Biologica Cracoviensia. Series Botanica
|
2010
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tom 52
|
nr 1
61-66
This work examined the distribution of necrosis on stems of two cultivars of potato (Ania, Glada) with different levels of resistance to PVY infection. Potato virus Y particles and/or cytoplasmic (CI) and amorphous inclusions (AI) were identified in insert and offshoot potato cells of susceptible cv. Glada. Cytoplasmic inclusions were not observed in insert and offshoot stems of resistant cv. Ania, although there were numerous deformations, degeneration and tissue necrosis. It was found that (1) necrotic reactions were the form of plant cell response for both the PVY-resistant and susceptible cultivars, (2) development of necrosis in vascular tissue did not prevent the pathogen from spreading outside the necrotic region in the less resistant cultivar (Glada), and (3) extreme resistance to PVY in potato plants, determined by the Rysto gene, was manifested in the absence of virus particles and cytoplasmic inclusions in infected plant cells.
7

Ultrastructural studies of plasmodesmatal and vascular translocation of tobacco rattle virus (TRV) in tobacco and potato

81%
Garbaczewska G., Otulak K., Chouda M., Chrzanowska M.
Acta Physiologiae Plantarum
|
2012
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tom 34
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nr 3
The studies focus on an ultrastructural analysis of the phenomenon of intercellular and systemic (vascular) transport of tobacco rattle virus (TRV) in tissues of the infected plants. TRV is a dangerous pathogen of cultivated and ornamental plants due to its wide range of plant hosts and continuous transmission by vectors—ectoparasitic nematodes. Two weeks after infection with the PSG strain of TRV, tobacco plants of the Samsun variety and potato plants of the Glada variety responded with spot surface necroses on inoculated leaf blades. Four weeks after the infection a typical systemic response was observed on tobacco and potato leaves, necroses on stems and lesions referred to as corky ringspot. Ultrastructural analysis revealed the presence of two types of TRV virions: capsidated and non-capsidated forms in tobacco and potato tissues. In the protoplast area, viral particles either occurred in a dispersed form or they formed organised inclusions of virions. We demonstrated for the first time the presence of non-capsidated-type TRV in the vicinity of and inside plasmodesmata. Capsidated particles of TRV were observed in intercellular spaces of the tissues of aboveground and underground organs. Expanded apoplast area was noted at the cell wall, with numerous dispersed noncapsidated-type TRV particles. These phenomena suggest active intercellular transport. Our ultrastructure studies showed for the first time that xylem can be a possible route of TRV systemic transport. We demonstrated that both capsidated and non-capsidated virions, of varied length, participate in long-distance transport. TRV virions were more often documented in xylem (tracheary elements and parenchyma) than in phloem. Non-capsidated TRV particles were observed inside tracheary elements in a dispersed form and in regular arrangements in potato and tobacco xylem. The presence of TRV virions inside the bordered pits was demonstrated in aboveground organs and in the root of the tested plants. We documented that both forms of TRV virions can be transported systemically via tracheary elements of xylem.
8
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Content available

Phylogenetic analysis of PDV movement protein compared to Bromoviridae members as justification of possible intercellular movement

81%
Koziel E., Otulak K., Garbaczewska G.
Acta Biologica Cracoviensia. Series Botanica
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2015
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tom 57
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nr 2
9

The pathogenesis of Tobacco (Nicotiana tabacum) cv. samsun infected with Tobacco rattle virus (TRV)

81%
Garbaczewska G., Otulak K., Chouda M.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr Suppl.1
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