Wyniki wyszukiwania

1

Sterowanie morfogeneza roslin w kulturach in vitro

100%

Burza W.

Kosmos

|

1995

|

tom 44

|

nr 3-4

691-701

2

Kultura in vitro i biotechnologia - spelnione nadzieje?

63%

Malepszy S., Burza W.

Biotechnologia

|

2010

|

nr 2

10-17

3

Uzyskiwanie roślin transgenicznych wolnych od genów selekcyjnych

51%

Zuzga S., Burza W., Malepszy S.

Biotechnologia

|

2003

|

nr 4

4

Transformacja u ogorka Cucumis sativus L.

51%

Szwacka M., Burza W., Palucha A., Malepszy S.

Biotechnologia

|

1997

|

nr 4

20-26

5

Shoot meristem differentiation in shoot primordia culture (SPC) obtained from root primordia culture (RPC) of Solanum lycopersicoides Dun.

51%

Tylicki A., Burza W., Kuras M., Malepszy S.

Acta Physiologiae Plantarum

|

2008

|

tom 30

|

nr 1

We present a unique example of conversion of the morphogenesis type (from rhisogenesis to shoot organogenesis) in in vitro cultures of Solanum lycopersicoides. Liquid shoot primordia cultures (SPC) were obtained from root primordia culture (RPC) on two kinds of MS-based media with BA. The first SMS₈, contained a full set of organic compounds; the second, ²SMS₈, was devoid of organic compounds except sucrose and edamine. Two weeks after passage of RPC onto both the media, disintegration of root primordia and cell aggregates began. After 8 weeks of cultivation on SMS₈ and ²SMS₈ media, SPC aggregates developed from meristematic cells located near the vascular tissue of disintegrating RPC aggregates. Initiation of shoot meristems started from meristematic cells centers which were localised under the surface of the newly formed SPC aggregates. The change in the type of morphogenesis occurred faster on medium SMS₈, but the SPC on medium ²SMS₈ was characterized by more frequent formation of shoots and plants (45% of aggregates, in the case of SPC on medium SMS₈, and 90% in the case of SPC on medium ²SMS₈). This fact was correlated with the structural organization of the meristematic centers. Our results indicate that an RPC system has high morphogenetic potential due to the continual presence of meristematic cells. The change in the type of morphogenesis was followed by a rebuilding of the aggregate structure on the basis of the meristematic cells already existing in RPC, which gave rise to SPC aggregates from which shoot meristems developed.

6

Kierowanie morfogeneza w kulturach zawiesinowych Solanum lycopersicoides Dun. III. Kultura primordiow pedowych

51%

Burza W., Dziadczyk E., Tylicki A., Malepszy S.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

441-442

7

Cytohistological analysis of somatic embryogenesis in cucumber [Cucumis sativus L.].II.Natural fluorescence and direct somatic embryogenesis from protoplasts

51%

Burza W., Wozniak B., Tarkowska J. A., Malepszy S.

Acta Societatis Botanicorum Poloniae

|

1994

|

tom 63

|

nr 3-4

265-268

8

Regeneracja roslin z protoplastow Solanum lycopersicoides Dun.

51%

Dziadczyk E., Burza W., Malepszy S.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

449-452

9

Direct regeneration from leaf explants of cucumber [Cucumis sativus L.] haploid

51%

Faris N. M., Burza W., Malepszy S., Niemirowicz-Szczytt K.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

249-252

10

Mikrowstrzeliwanie nie zmniejsza zdolnosci primordiow pedowych ogorka do regeneracji

51%

Czarnak M., Burza W., Rakoczy-Trojanowska M.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

513-516

11

Nowa metoda klonalnego namnazania ogorka [Cucumis sativus L.]

51%

Burza W., Kondo K., Ladyzynski M.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

157-160

12

Cytohistological analysis of somatic embryogenesis in cucumber [Cucumis sativus L.]. I. Comparison of cell suspension containing and lacking natural fluorescence with in vivo developing embryos

51%

Tarkowska J. A., Brzostecka D., Burza W., Malepszy S.

Acta Societatis Botanicorum Poloniae

|

1994

|

tom 63

|

nr 3-4

259-263

13

Regeneracja roslin z ustalonej kultury zawiesinowej pomidora [Lycopersicon esculentum Mill.]

51%

Burza W., Dziadczyk E., Malepszy S.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1997

|

tom 50

447-448

14

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Characterization of a cucumber [Cucumis sativus L.] somaclonal variant with paternal inheritance

51%

Malepszy S., Burza W., Smiech M.

Journal of Applied Genetics

|

1996

|

tom 37

|

nr 1

Regeneration of cucumber plants from leaf explants resulted in a new species phenotype designated mosaic (msc). It is characterized by two types of spots on the leaves (zucchini-like and chlorophyllous) and has many altered morphological and physiological properties including slower growth, smaller organs, poorly germinating or empty seeds and a smaller number of flowers per node. In msc plants the shape of the first leaf is always altered, and in about 76% of the flowers the crown is reduced and distorted to a varying degree. Chloroplasts of the zucchini-like sectors are filled with large starch grains, and some of the embryos die at various stages of development. The msc phenotype is transmitted uniparentally only by the male plant and no segregation is observed in the F2 and subsequent generations. Possible mechanisms responsible for the msc phenotype and its transmission are discussed.

15

Agrobacterium tumefaciens - mediated cucumber transformation with thaumatin II cDNA

51%

Szwacka M., Morawski M., Burza W.

Journal of Applied Genetics

|

1996

|

tom 37A

16

Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation

51%

Burza W., Wochniak P., Wroblewski T., Malepszy S.

Journal of Applied Genetics

|

1995

|

tom 36

|

nr 1

The paper presents a new way of obtaining viable and very homogeneous cucumber protoplasts. Protoplasts from cells formed in the shoot tip meristem culture were isolated from suspension. Plasmid pBI121 was introduced using impulse electric field. Effectiveness of transformation process was determined by the transient expression of ß-glucuronidase (GUS) gene, controlled by promotor 35S. The activity of ß-glucuronidase enzyme as a product of GUS reporter gene was estimated by fluorimetric method (JEFFERSON 1987). Parameters of electroporation process were optimized. The transient expression of GUS gene was measured 24 h after electroporation. The highest effectiveness of transformation process was achieved using three electric impulses at the initial voltage of 250-350 V at 10-sec. intervals as a result of discharging a 140 µF capacitor and 50-70 µg × cm⁻³ plasmid DNA in the presence of 50 µg × cm⁻³ carrier DNA. The system presented is an effective method of exogenic DNA transfer, which is indicated by a high transient expression of the reporter gene. In comparison to Agrobacterium tumefaciens and A. rhizogenes, this alternative method of gene transfer can be used for obtaining transgenic cucumber plants.

17

Plant recovery from bud micro-explants of squash [Cucurbita maxima Duch.]

51%

Ladyzynski M., Burza W., Malepszy S.

Vegetable Crops Research Bulletin

|

2002

|

tom 56

18

Optimisation of particle bombardment parameters for shoot primordia explants of Cucumis sativus L.

51%

Czarnak M., Burza W., Filipecki M., Rakoczy-Trojanowska M.

Journal of Applied Genetics

|

1996

|

tom 37A

19

The role of the tubulin cytoskeleton of Solanum lycopersicoides Dun. protoplasts in the course of diploid plant regeneration

51%

Tylicki A., Burza W., Malepszy S., Kuras M.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

20

Cucumber embryogenetic haploid suspension cultures

51%

Klos M., Kondo K., Niemirowicz-Szczytt K., Burza W.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

Ograniczanie wyników

Sterowanie morfogeneza roslin w kulturach in vitro

Kultura in vitro i biotechnologia - spelnione nadzieje?

Uzyskiwanie roślin transgenicznych wolnych od genów selekcyjnych

Transformacja u ogorka Cucumis sativus L.

Shoot meristem differentiation in shoot primordia culture (SPC) obtained from root primordia culture (RPC) of Solanum lycopersicoides Dun.

Kierowanie morfogeneza w kulturach zawiesinowych Solanum lycopersicoides Dun. III. Kultura primordiow pedowych

Cytohistological analysis of somatic embryogenesis in cucumber [Cucumis sativus L.].II.Natural fluorescence and direct somatic embryogenesis from protoplasts

Regeneracja roslin z protoplastow Solanum lycopersicoides Dun.

Direct regeneration from leaf explants of cucumber [Cucumis sativus L.] haploid

Mikrowstrzeliwanie nie zmniejsza zdolnosci primordiow pedowych ogorka do regeneracji

Nowa metoda klonalnego namnazania ogorka [Cucumis sativus L.]

Cytohistological analysis of somatic embryogenesis in cucumber [Cucumis sativus L.]. I. Comparison of cell suspension containing and lacking natural fluorescence with in vivo developing embryos

Regeneracja roslin z ustalonej kultury zawiesinowej pomidora [Lycopersicon esculentum Mill.]

Characterization of a cucumber [Cucumis sativus L.] somaclonal variant with paternal inheritance

Agrobacterium tumefaciens - mediated cucumber transformation with thaumatin II cDNA

Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation

Plant recovery from bud micro-explants of squash [Cucurbita maxima Duch.]

Optimisation of particle bombardment parameters for shoot primordia explants of Cucumis sativus L.

The role of the tubulin cytoskeleton of Solanum lycopersicoides Dun. protoplasts in the course of diploid plant regeneration

Cucumber embryogenetic haploid suspension cultures